標(biāo)題: Titlebook: Mesenchymal Stem Cells; Methods and Protocol Massimiliano Gnecchi Book 2016Latest edition Springer Science+Business Media New York 2016 Mes [打印本頁] 作者: MIFF 時間: 2025-3-21 16:52
書目名稱Mesenchymal Stem Cells影響因子(影響力)
作者: 積云 時間: 2025-3-21 23:19 作者: commune 時間: 2025-3-22 04:04 作者: brassy 時間: 2025-3-22 06:19
Isolation, Expansion, and Immortalization of Human Adipose-Derived Mesenchymal Stromal Cells from Bive reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.作者: LINES 時間: 2025-3-22 09:58
Mesenchymal Stromal Cells in Hematopoietic Stem Cell Transplantationafe; moreover, promising preliminary results in terms of efficacy have been reported in some clinical trials. This chapter focuses on recent advances in MSC therapy by reporting on the most important relevant studies in the field of HSCT.作者: 天氣 時間: 2025-3-22 14:33 作者: 一罵死割除 時間: 2025-3-22 18:34 作者: 小歌劇 時間: 2025-3-22 22:46 作者: Kindle 時間: 2025-3-23 02:08 作者: 混亂生活 時間: 2025-3-23 05:52
he theoretical rules given in these books are routinely used by physicists to compute observable quantities. Their predictions can then be compared with experimental data. There is no fundamental disagreement among physicists on how to use the theory for these practical purposes. However, there are 作者: Affirm 時間: 2025-3-23 10:13
Maria Ester Bernardo M.D., Ph.D.,Franco Locatellihe theoretical rules given in these books are routinely used by physicists to compute observable quantities. Their predictions can then be compared with experimental data. There is no fundamental disagreement among physicists on how to use the theory for these practical purposes. However, there are 作者: 四溢 時間: 2025-3-23 15:02 作者: Enliven 時間: 2025-3-23 20:16 作者: 轉(zhuǎn)折點(diǎn) 時間: 2025-3-24 00:45 作者: myalgia 時間: 2025-3-24 04:13
Luigi Balducci,Giulio Alessandriimental measurements in accuracy on a routine basis. Only a decade ago, such a statement would be valid only with severe qualifi- tions as high-level quantum-chemical calculations were feasible only for molecules composed of a few atoms. Improvements in both hardware performance and the level of sop作者: preeclampsia 時間: 2025-3-24 07:57 作者: Haphazard 時間: 2025-3-24 11:14 作者: 人類的發(fā)源 時間: 2025-3-24 16:44
Mesenchymal Stromal Cells in Hematopoietic Stem Cell Transplantation ex vivo for clinical use. Thanks to their secretion of growth factors, immunomodulatory properties and cell-to-cell interactions, MSCs play a key role in the regulation of hematopoiesis and in the modulation of immune responses against allo- and autoantigens. In light of these properties, MSCs have作者: 使習(xí)慣于 時間: 2025-3-24 20:33
Bone Tissue Engineering: Past–Present–Futuretightly regulated sequences of molecular and cellular events which recapitulate tissue formation seen during embryogenesis. Still in some instances, bone regeneration does not occur properly (i.e. critical size lesions) and an appropriate therapeutic intervention is necessary. Successful replacement作者: rheumatology 時間: 2025-3-25 01:50
Mesenchymal Stem Cells for Osteochondral Tissue Engineeringrrow and fat aspirates) and they are able to form a range of mesenchymal tissues, in vitro and in vivo. We focus here on the use of MSCs for engineering of cartilage, bone, and complex osteochondral tissue constructs, using protocols that replicate some aspects of natural mesodermal development. For作者: Ossification 時間: 2025-3-25 05:44
Mesenchymal Stem Cells in Cardiologyinfarction (heart attack) each year in the United States alone with roughly one-third resulting in death. There is therefore a major need for developing new and effective strategies to promote cardiac repair. Intramyocardial transplantation of mesenchymal stem cells (MSCs) has emerged as a leading c作者: OPINE 時間: 2025-3-25 09:16
Mesenchymal Stem Cells in Kidney Repairney disease (CKD) in a substantial percentage of patients besides other organ dysfunctions. To date, the mortality rate per year for AKI exceeds 50 % at least in patients requiring early renal replacement therapy and is higher than the mortality for breast and prostate cancer, heart failure and diab作者: Precursor 時間: 2025-3-25 13:18 作者: Priapism 時間: 2025-3-25 17:53
Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repaire principal mechanism underlying their therapeutic action. However, several studies have now shown that both these mechanisms are very inefficient. The low MSC engraftment rate documented in injured areas also refutes the hypothesis that MSC repair tissue damage by replacing cell loss with newly dif作者: cushion 時間: 2025-3-25 23:04
Protocols for , Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adlications for cell therapy and reparative biotechnologies. MSC have been originally isolated from the bone marrow (BM-MSC), but they have been found also in other tissues such as adipose tissue, cord blood, synovium, skeletal muscle, and lung. MSC are able to differentiate in vitro and in vivo into 作者: Junction 時間: 2025-3-26 01:32
Colony Forming Unit Assaystheir wound healing properties in over 350 clinical trials worldwide. MSCs from various tissues such as adipose, bone, and others are currently being studied in clinical trials in indications for ischemic, inflammatory, autoimmune, and degenerative disorders. As a result, numerous isolation protocol作者: Keratin 時間: 2025-3-26 04:30 作者: CUMB 時間: 2025-3-26 08:50 作者: CANDY 時間: 2025-3-26 13:10 作者: 故意釣到白楊 時間: 2025-3-26 19:47
Isolation, Culture, and Phenotypic Characterization of Mesenchymal Stromal Cells from the Amniotic Mcells to be used in cell therapy approaches due to its putative stem cell potential associated with its early embryonic origin and its immunomodulatory potential associated with its role in fetomaternal tolerance..Within the human amniotic membrane, it is possible to isolate two main cell population作者: sparse 時間: 2025-3-27 00:05 作者: 排他 時間: 2025-3-27 04:31
Isolation, Expansion, and Immortalization of Human Adipose-Derived Mesenchymal Stromal Cells from Bi regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span..Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encounte作者: definition 時間: 2025-3-27 06:51
Optimization of Mesenchymal Stem Cells to Increase Their Therapeutic Potentialsing therapeutic strategy to improve healing of the ischemic heart, repopulate the injured myocardium, and restore cardiac function. However, clinical usefulness is impacted by the quality and quantity of delivered cells, the suboptimal manipulations prior to transplantation, and the general poor vi作者: pessimism 時間: 2025-3-27 11:47
Directed Differentiation of Human-Induced Pluripotent Stem Cells to Mesenchymal Stem Cellshelial cells, smooth muscle cells, bone, cartilage, and lipid cells. This protocol describes in detail how to perform highly efficient, lineage-specific differentiation of human-induced pluripotent stem cells (iPSCs) with an MSCs fate. The approach uses a clinically compliant protocol with chemicall作者: 愛管閑事 時間: 2025-3-27 17:21 作者: Infuriate 時間: 2025-3-27 19:55
Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/631013.jpg作者: Carminative 時間: 2025-3-27 22:53 作者: macabre 時間: 2025-3-28 03:08 作者: 整體 時間: 2025-3-28 06:59
https://doi.org/10.1007/978-1-4939-3584-0Mesenchymal stem cells (MSC); cell therapy; isolation; characterization; MSC secretome; clinical use作者: 特征 時間: 2025-3-28 10:54 作者: 掃興 時間: 2025-3-28 17:51
approach is pragmatic and strictly instrumentalist. This attitude will undoubtedly antagonize some readers, but it has its own logic: quantum phenomena do not occur in a Hilbert space, they occur in a laboratory.978-0-7923-3632-7978-0-306-47120-9Series ISSN 0168-1222 Series E-ISSN 2365-6425 作者: Gullible 時間: 2025-3-28 21:57 作者: aesthetician 時間: 2025-3-29 01:43
Rodolfo Quarto,Paolo Giannoniapproach is pragmatic and strictly instrumentalist. This attitude will undoubtedly antagonize some readers, but it has its own logic: quantum phenomena do not occur in a Hilbert space, they occur in a laboratory.978-0-7923-3632-7978-0-306-47120-9Series ISSN 0168-1222 Series E-ISSN 2365-6425 作者: Confirm 時間: 2025-3-29 03:55 作者: NADIR 時間: 2025-3-29 09:54
Ian A. White Ph.D.,Cristina Sanina M.D.,Wayne Balkan Ph.D.,Joshua M. Hare M.D.approach is pragmatic and strictly instrumentalist. This attitude will undoubtedly antagonize some readers, but it has its own logic: quantum phenomena do not occur in a Hilbert space, they occur in a laboratory.978-0-7923-3632-7978-0-306-47120-9Series ISSN 0168-1222 Series E-ISSN 2365-6425 作者: 正面 時間: 2025-3-29 12:35 作者: 能夠支付 時間: 2025-3-29 17:59 作者: 極小量 時間: 2025-3-29 19:58
Qizhou Lian M.D., Ph.D.,Yuelin Zhang M.D., Ph.D.,Xiaoting Liang M.D.,Fei Gao M.D.,Hung-Fat Tse M.D.,f electron correlation used in conjunction with extrapolative schemes to semiempirical methods, these approaches have well-known advantages and shortcomings that determine their usefulness in studies of particular types of chemical species. The growing number of published computational schemes and their varia978-1-4020-0424-7978-0-306-47632-7作者: 眨眼 時間: 2025-3-30 03:35 作者: consolidate 時間: 2025-3-30 04:21
Mesenchymal Stem Cells in Cardiologyferentiation into cardiomyocytes, endothelial cells, and vascular smooth muscle cells, although the relative contribution of trilineage differentiation and paracrine effectors on cardiac repair remains the subject of active investigation.作者: DEI 時間: 2025-3-30 08:25 作者: resistant 時間: 2025-3-30 13:51 作者: 多產(chǎn)子 時間: 2025-3-30 19:23
Protocols for , Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and AdFor all these reasons, the scientific appeal of MSC is constantly on the rise..The identity of MSC is currently based on three main criteria: plastic-adherence capacity, defined epitope profile, and capacity to differentiate in vitro into osteocytes, chondrocytes, and adipocytes. Here, we describe s作者: Proclaim 時間: 2025-3-31 00:45
Colony Forming Unit Assayslonies when plated at low densities. The efficiency with which they form colonies still remains an important assay for the quality of cell preparations. To assess the quality of cell preparations, two different colony forming unit (CFU) assays are also provided.作者: 哎呦 時間: 2025-3-31 00:56
Methods and Strategies for Lineage Tracing of Mesenchymal Progenitor Cellsampered by a lack of suitable Cre lines in which to effectively genetically mark and follow the fate and activity of these cells in development, growth, homeostasis and following injury or in disease. The emergence of several new genetic lines is enabling us to now address critical questions regardi作者: DUST 時間: 2025-3-31 06:04
Isolation of Mouse Bone Marrow Mesenchymal Stem Cellsthese cells in atmospheric oxygen selects for immortalized clones that lack a functional p53 protein. Herein, we describe a protocol for the isolation of primary MSCs from mouse bone marrow that couples immunodepletion with culture in a low-oxygen environment and affords high purity and yield while 作者: Angioplasty 時間: 2025-3-31 10:21 作者: Abominate 時間: 2025-3-31 13:22 作者: Scintillations 時間: 2025-3-31 17:40 作者: 懦夫 時間: 2025-3-31 23:39 作者: 不持續(xù)就爆 時間: 2025-4-1 02:12
1064-3745 rotocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls...Authoritative and cutting-edge, .Mesenchymal Stem Cells: Methods and Protocols, Second Edition., aims to ensure suc978-1-4939-8085-7978-1-4939-3584-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Dappled 時間: 2025-4-1 06:07 作者: 過去分詞 時間: 2025-4-1 13:20
Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.,Patrizia Danieli Ph.D.,Giuseppe Malpasso Ph.D.,Maria Chia作者: 過時 時間: 2025-4-1 15:36
Maria Chiara Ciuffreda Ph.D.,Giuseppe Malpasso Ph.D.,Paola Musarò M.Sc.,Valentina Turco M.Sc.,Massim作者: 制造 時間: 2025-4-1 20:46