標(biāo)題: Titlebook: Membrane Trafficking; Methods and Protocol Jingshi Shen Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license [打印本頁] 作者: Hemochromatosis 時(shí)間: 2025-3-21 18:26
書目名稱Membrane Trafficking影響因子(影響力)
書目名稱Membrane Trafficking影響因子(影響力)學(xué)科排名
書目名稱Membrane Trafficking網(wǎng)絡(luò)公開度
書目名稱Membrane Trafficking網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Membrane Trafficking被引頻次
書目名稱Membrane Trafficking被引頻次學(xué)科排名
書目名稱Membrane Trafficking年度引用
書目名稱Membrane Trafficking年度引用學(xué)科排名
書目名稱Membrane Trafficking讀者反饋
書目名稱Membrane Trafficking讀者反饋學(xué)科排名
作者: 無可爭辯 時(shí)間: 2025-3-21 20:23 作者: 割讓 時(shí)間: 2025-3-22 01:24 作者: Detonate 時(shí)間: 2025-3-22 04:34
Membrane Trafficking978-1-0716-2209-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: reserve 時(shí)間: 2025-3-22 12:36
Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp TechniqueWhole-cell patch clamping is a standard method to monitor the secretion of synaptic vesicles. In this chapter, we describe the basic steps of whole-cell patch clamping for measuring synaptic exocytosis, aiming to provide reference for researchers who are new to this field.作者: SOBER 時(shí)間: 2025-3-22 14:25
https://doi.org/10.1007/978-1-0716-2209-4Endomembrane systems; Exocytic pathway; Endocytic pathway; Membrane biology; CRISPR genetic screens作者: collagen 時(shí)間: 2025-3-22 21:06
Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook scently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).作者: craven 時(shí)間: 2025-3-23 00:11
In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusionoteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.作者: xanthelasma 時(shí)間: 2025-3-23 05:17
Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)ws for visualization of membrane dynamics at the cell surface with superb spatiotemporal resolution. In this chapter, TIRFM is used to record and analyze exocytosis of single glucose transporter-4 (GLUT4) containing vesicles in 3T3-L1 adipocytes.作者: 賠償 時(shí)間: 2025-3-23 06:22
Julia Dancourt,Grégory Lavieuwith detailed examples and exercises at the end of each chap.Inventories are prevalent everywhere in the commercial world, whether it be in retail stores, manufacturing facilities, government stockpile material, Federal Reserve banks, or even your own household. This textbook examines basic mathemat作者: Offstage 時(shí)間: 2025-3-23 11:47 作者: browbeat 時(shí)間: 2025-3-23 15:44 作者: Homocystinuria 時(shí)間: 2025-3-23 20:56
Syed Mukhtar Ahmed, Federal Reserve banks, or even your own household. This textbook examines basic mathematical techniques used to sufficiently manage inventories by using various computational methods and mathematical models. Such models discussed include: EOQ model and extensions, power-of-two models, single and m作者: 討人喜歡 時(shí)間: 2025-3-23 23:48
Kunrong Mei,Di-Ao Liu,Wei Guo, Federal Reserve banks, or even your own household. This textbook examines basic mathematical techniques used to sufficiently manage inventories by using various computational methods and mathematical models. Such models discussed include: EOQ model and extensions, power-of-two models, single and m作者: Ballerina 時(shí)間: 2025-3-24 06:12
Fei Kang,Herbert Y. Gaisano, Federal Reserve banks, or even your own household. This textbook examines basic mathematical techniques used to sufficiently manage inventories by using various computational methods and mathematical models. Such models discussed include: EOQ model and extensions, power-of-two models, single and m作者: CRAFT 時(shí)間: 2025-3-24 06:54
Jihong Gong,Xiaofei Yang,Cong Mawith detailed examples and exercises at the end of each chap.Inventories are prevalent everywhere in the commercial world, whether it be in retail stores, manufacturing facilities, government stockpile material, Federal Reserve banks, or even your own household. This textbook examines basic mathemat作者: Baffle 時(shí)間: 2025-3-24 12:23
Tong Wang,Frédéric A. Meunier, Federal Reserve banks, or even your own household. This textbook examines basic mathematical techniques used to sufficiently manage inventories by using various computational methods and mathematical models. Such models discussed include: EOQ model and extensions, power-of-two models, single and m作者: Glutinous 時(shí)間: 2025-3-24 15:35
Tamara Basta,Garry P. Morgan,Eileen T. O’Toole,Nalini R. Rao,Jeffrey N. Savas,Michael H. B. Stowell, Federal Reserve banks, or even your own household. This textbook examines basic mathematical techniques used to sufficiently manage inventories by using various computational methods and mathematical models. Such models discussed include: EOQ model and extensions, power-of-two models, single and m作者: 受傷 時(shí)間: 2025-3-24 21:22 作者: 閑逛 時(shí)間: 2025-3-25 00:53
Yingke Xu,Luhong Jin,Derek Toomre to International Trade, the author draws from his practical and teaching experience to give a comprehensive introduction to the key areas of law that apply in international business...For the benefit of readers unfamiliar with the English legal system and the many associated branches of English civ作者: compose 時(shí)間: 2025-3-25 06:27
to International Trade, the author draws from his practical and teaching experience to give a comprehensive introduction to the key areas of law that apply in international business...For the benefit of readers unfamiliar with the English legal system and the many associated branches of English civ作者: 不在灌木叢中 時(shí)間: 2025-3-25 07:56 作者: 雄辯 時(shí)間: 2025-3-25 15:24
Shifeng Wang,Chun Wan,Galen T. Squiers,Jingshi Shen to International Trade, the author draws from his practical and teaching experience to give a comprehensive introduction to the key areas of law that apply in international business...For the benefit of readers unfamiliar with the English legal system and the many associated branches of English civ作者: Adulterate 時(shí)間: 2025-3-25 16:25 作者: A精確的 時(shí)間: 2025-3-25 21:12 作者: 發(fā)生 時(shí)間: 2025-3-26 02:47
Priyanka Sharma,Sameena Parveen,Sunando Datta to International Trade, the author draws from his practical and teaching experience to give a comprehensive introduction to the key areas of law that apply in international business...For the benefit of readers unfamiliar with the English legal system and the many associated branches of English civ作者: Binge-Drinking 時(shí)間: 2025-3-26 04:55 作者: 檢查 時(shí)間: 2025-3-26 10:37 作者: 交響樂 時(shí)間: 2025-3-26 13:29
Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook scently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).作者: 最初 時(shí)間: 2025-3-26 17:48
Spatial and Temporal Control of Protein Secretion with Lightmains an important problem in cell biology. One powerful approach for elucidating the trafficking route of a specific protein is to sequester it following synthesis in the endoplasmic reticulum and trigger its release with an externally applied cue. Combined with fluorescent probes, this approach ca作者: MEEK 時(shí)間: 2025-3-27 00:42
Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editingosed proteins. Biochemical and cell biological studies of vesicle trafficking often require the introduction of epitope tags or fluorescent protein markers for protein purification and tracking in cells. Previously, such tagging experiments in mammalian cells mainly used overexpression systems, whic作者: BULLY 時(shí)間: 2025-3-27 01:10
Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting vesicles to the plasma membrane. In the assay, we use a plasmid that encodes a fusion protein of the mitochondria protein Tom20 and Sec3 N-terminally tagged with the florescence protein mCherry, and coexpress the plasmid in yeast cells with CIT1-GFP, a marker protein of mitochondria. We then detect作者: Amplify 時(shí)間: 2025-3-27 05:57 作者: GLARE 時(shí)間: 2025-3-27 13:16
Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse–Chase Labeling in Culafficking. Further, hippocampal neurons exhibit “en passant” presynapses that may confound the analysis of long-range retrograde axonal transport. To solve these issues, we and others have developed microfluid-based methods to specifically follow the fates of the retrograde axonal cargoes following 作者: 新鮮 時(shí)間: 2025-3-27 14:06
Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapseynaptic vesicles, Nissl bodies, and early endosomes. Here, we describe methods for the preparation of select murine brain regions for high-pressure freezing, freeze substitution, and EM tomographic analysis of synaptic structures. The method uses fresh brain slices prepared using a vibratome and bio作者: 財(cái)產(chǎn) 時(shí)間: 2025-3-27 19:06
In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusionoteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion eve作者: 染色體 時(shí)間: 2025-3-28 01:57
Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)ws for visualization of membrane dynamics at the cell surface with superb spatiotemporal resolution. In this chapter, TIRFM is used to record and analyze exocytosis of single glucose transporter-4 (GLUT4) containing vesicles in 3T3-L1 adipocytes.作者: 污穢 時(shí)間: 2025-3-28 02:21
Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platncoded tags with tomography enables the specific targeting and detection of identified proteins inside cells. Here, we describe a method for attaching metal-binding gold nanoparticles to proteins genetically tagged with hexa-histidine sequences. We apply this strategy to visualize the position of in作者: Hemiplegia 時(shí)間: 2025-3-28 08:55 作者: 險(xiǎn)代理人 時(shí)間: 2025-3-28 11:15 作者: Clumsy 時(shí)間: 2025-3-28 17:06 作者: mutineer 時(shí)間: 2025-3-28 21:02
Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approachesis exclusively required to transport these cargoes through various intracellular routes for their delivery to the site of action. Among these, recycling of cargoes to the plasma membrane is a crucial pathway for the efficient functioning of the cell. Hence, endosomal cargo recycling assays are cruci作者: 調(diào)色板 時(shí)間: 2025-3-29 01:00
Book 2022tro. biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pat作者: Adenoma 時(shí)間: 2025-3-29 06:33 作者: intuition 時(shí)間: 2025-3-29 09:08
Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editingthe advent of CRISPR technologies, epitope tags and fluorescent proteins can now be introduced into endogenous proteins in almost any cell type that are proliferating in culture. This chapter describes approaches for inserting tags at the endogenous loci of genes, with the vesicle tethering protein complex, exocyst, as an example.作者: 旁觀者 時(shí)間: 2025-3-29 11:45
Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluoresocking and fusion dynamics and can also map its regulators with high spatiotemporal resolution. Here, we describe the basic principles and practical implementation of a fast dual-color TIRF microscope, detailing a how-to guide on imaging and analysis of insulin granule dynamics in human β-cells.作者: 遭受 時(shí)間: 2025-3-29 18:23
Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platund that this combined method can label and localize proteins with isotropic high precision to generate quantitative maps of protein positions in and around trafficking organelles at the inner plasma membrane of mammalian cells.作者: Intellectual 時(shí)間: 2025-3-29 20:44 作者: Obloquy 時(shí)間: 2025-3-30 02:12
Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting recruits the other exocyst subunits and secretory vesicles to a target membrane, suggesting that it plays a pivotal role in vesicle tethering. This approach is likely appropriate for studying other tethering complexes at their specific stages of trafficking and may also be used in other eukaryotic cells such as the cultured mammalian cells.作者: 金桌活畫面 時(shí)間: 2025-3-30 07:20 作者: DRAFT 時(shí)間: 2025-3-30 11:37
Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargohe Golgi. When traffic resumes upon temperature block release, kinetics of transport can be assessed by confocal microscopy through colocalization of the cargo with cis- and trans-Golgi markers. This method allows for testing various modes of intra-Golgi transports and can be adapted to investigate other steps of the secretory pathway.作者: 遠(yuǎn)地點(diǎn) 時(shí)間: 2025-3-30 15:31 作者: 有助于 時(shí)間: 2025-3-30 19:19
1064-3745 expertsThis detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to .in vitro. biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, whi作者: ANT 時(shí)間: 2025-3-30 22:16
Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention Systemctive Hook (RUSH) system, a highly specific and sensitive tracking system with a high spatiotemporal resolution. Taking GLUT4 and GLUT1 as examples of unconventionally and conventionally secreted cargo respectively, we describe the steps to clone the cargoes in the RUSH vector and follow and quantif作者: BORE 時(shí)間: 2025-3-31 02:12
Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse–Chase Labeling in Culailable microfluidic device to enable the labeling and tracking of retrograde axonal carriers, including (1) how to culture and transfect rat hippocampal neurons in the microfluidic device; (2) how to perform pulse–chase to label specific populations of retrograde axonal carriers; and (3) how to con作者: Debrief 時(shí)間: 2025-3-31 05:04 作者: Accommodation 時(shí)間: 2025-3-31 12:21 作者: INERT 時(shí)間: 2025-3-31 13:37
Book 2022nown pitfalls.?.Authoritative and practical, .Membrane Trafficking: Methods and Protocols. provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs..作者: 減去 時(shí)間: 2025-3-31 19:18 作者: 注射器 時(shí)間: 2025-4-1 00:32 作者: Basilar-Artery 時(shí)間: 2025-4-1 04:55
Kunrong Mei,Di-Ao Liu,Wei Guoer approaches the book can be inconsequential. It contains both deterministic and stochastic models along with algorithms that can be employed to find solutions to a variety of inventory control problems...Key topics include:..* Economic order quantity (EOQ) model..* Power-of-two policies..* Dynamic作者: 開始發(fā)作 時(shí)間: 2025-4-1 09:07
Fei Kang,Herbert Y. Gaisanoer approaches the book can be inconsequential. It contains both deterministic and stochastic models along with algorithms that can be employed to find solutions to a variety of inventory control problems...Key topics include:..* Economic order quantity (EOQ) model..* Power-of-two policies..* Dynamic
