標(biāo)題: Titlebook: Matrix Metalloproteinase Protocols; Ian M. Clark Book 2010Latest edition Humana Press 2010 Escherichia coli.Microarray.Nucleotide.PCR.Prot [打印本頁] 作者: 矜持 時間: 2025-3-21 17:20
書目名稱Matrix Metalloproteinase Protocols影響因子(影響力)
作者: FLIC 時間: 2025-3-21 23:59 作者: cyanosis 時間: 2025-3-22 03:19 作者: 不朽中國 時間: 2025-3-22 07:40
n einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: MAL 時間: 2025-3-22 11:29
Caroline J. Pennington,Dylan R. Edwardsn einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 哭得清醒了 時間: 2025-3-22 13:09 作者: aplomb 時間: 2025-3-22 17:15 作者: Visual-Field 時間: 2025-3-22 21:43
Yasunori Okadan einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 殺死 時間: 2025-3-23 05:14 作者: 缺陷 時間: 2025-3-23 06:14
n einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 破裂 時間: 2025-3-23 10:08
Vera Kn?uper,Gillian Murphyn einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: Pandemic 時間: 2025-3-23 14:20
Tim E. Cawston,Rachel L. Lakey,Andrew D. Rowann einführenden als auch einer weiterführenden Numerik-Vorlesung im Ingenieurstudium dienen und eignet sich darüber hinaus für die Numerikausbildung im Mathematikstudium..978-3-540-29883-0Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 作繭自縛 時間: 2025-3-23 18:53
1064-3745 ogy.? series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooti978-1-4939-5622-7978-1-60327-299-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 眼界 時間: 2025-3-24 01:49
Alejandro P. Ugalde,Gonzalo R. Ordó?ez,Pedro M. Quirós,Xose S. Puente,Carlos López-Otín作者: mendacity 時間: 2025-3-24 05:33
Sean E. Gill,Sean Y. Kassim,Timothy P. Birkland,William C. Parks作者: Thyroxine 時間: 2025-3-24 08:01 作者: 詳細(xì)目錄 時間: 2025-3-24 10:51
des Problems konvergieren. In den Ellipsoid-Methoden wird durch st?ndig verbesserte Einschlie?ungen mit Ellipsoiden eine L?sung durch ?u?ere Punkte approximiert. In der historischen Reihenfolge der in diesem Abschnitt betrachteten Methoden steht die Ellipsoid-Methode am Anfang. Mit ihrer Hilfe wurde作者: 高度 時間: 2025-3-24 16:51 作者: Neonatal 時間: 2025-3-24 19:41
Richard A. Williamsonen Splines in Abschnitt 8.6 erl?utert. In diesem Kapitel wird die Interpolation mit Splines in einem allgemeineren Rahmen behandelt. Ferner bietet Interpolation bei weitem nicht die einzige Strategie, Daten zu ?fitten“ oder eine Funktion zu approximieren. Insbesondere wenn Daten wie in vielen Anwend作者: Misgiving 時間: 2025-3-25 02:51 作者: photopsia 時間: 2025-3-25 07:02 作者: 一起 時間: 2025-3-25 08:52 作者: Sputum 時間: 2025-3-25 12:08 作者: 凹槽 時間: 2025-3-25 18:06 作者: 散布 時間: 2025-3-25 23:15
Caroline J. Pennington,Dylan R. Edwardslustrierende Beispiele helfen, den konkreten Ablauf von Meth.Dieses Lehrbuch bietet eine Einführung in alle wesentlichen grundlegenden numerischen Methodenbausteine, wobei in einem abschliessenden Kapitel die Kombination dieser Bausteine anhand komplexerer Anwendungsbeispiele illustriert wird. Den e作者: 不能約 時間: 2025-3-26 00:29 作者: AUGUR 時間: 2025-3-26 06:36 作者: 可憎 時間: 2025-3-26 11:21 作者: Lipoprotein(A) 時間: 2025-3-26 13:25 作者: Intrepid 時間: 2025-3-26 18:44 作者: 女上癮 時間: 2025-3-27 00:29 作者: 侵略者 時間: 2025-3-27 02:32 作者: jealousy 時間: 2025-3-27 08:50 作者: Optometrist 時間: 2025-3-27 10:34
Mouse Models of MMP and TIMP Functionis indeed performed by some family members. However, matrix degradation is possibly not the predominant function of these enzymes. Several studies have demonstrated that MMPs also act on a variety of non-matrix extracellular proteins, such as cytokines, chemokines, receptors, junctional proteins, an作者: 調(diào)整 時間: 2025-3-27 14:34
Expression of Recombinant Matrix Metalloproteinases in ,matrix (ECM). The role that the MMPs play in normal and pathological conditions has long been of interest. The mechanisms by which the MMPs cleave the different components of the ECM have been examined extensively. Some of these studies have been made possible, in part, by the ability to express rec作者: 紅腫 時間: 2025-3-27 21:39 作者: AUGUR 時間: 2025-3-27 22:17
Expression and Purification of Membrane-Type MMPsicellular proteolysis. Understanding the activity, regulation, and function of MT-MMPs would provide important insight for ongoing research in many diseases including cancer, fibrosis, and autoimmune diseases. In this chapter, we introduce the methods to express and purify recombinant MT-MMPs using 作者: panorama 時間: 2025-3-28 03:28
Refolding of TIMP-2 from , Inclusion Bodies provide the necessary post-translational modification apparatus to produce authentic TIMP but are comparatively slow and more expensive. This chapter describes the production of native TIMP-2 (both full-length and the N-terminal domain) from . by in vitro refolding. The technique allows high-level 作者: 尊嚴(yán) 時間: 2025-3-28 10:02 作者: 無價值 時間: 2025-3-28 13:48 作者: 代理人 時間: 2025-3-28 17:59 作者: SPASM 時間: 2025-3-28 19:47 作者: 混亂生活 時間: 2025-3-29 02:08
Methods for Studying Activation of Matrix Metalloproteinasesyme systems, including the matrix metalloproteinases (MMPs), serine proteinases, and cysteine proteinases. The majority of the soluble MMPs are synthesized as proenzymes which require extracellular activation in order to gain proteolytic activity and the analysis of their activation mechanism is a p作者: 溫室 時間: 2025-3-29 06:29
Assay of Matrix Metalloproteinases Against Matrix Substrateso be measured. The protocols described include the preparation of radiolabeled substrates, methods for the separation of degraded product from undegraded substrate, and methods for the activation of MMPs. The advantages and disadvantages of these methods are discussed in relation to immunoassays tha作者: 迅速飛過 時間: 2025-3-29 08:59
Metalloproteases and the Degradomeree families of metalloproteases: ADAMs (a disintegrin and metalloproteinase), ADAMTSs (ADAMs with thrombospondin domains), and MMPs (matrix metalloproteinases) which have a growing relevance in a number of human pathologies including cancer, arthritis, neurodegenerative disorders, and cardiovascular diseases.作者: 蒼白 時間: 2025-3-29 12:12
Expression of Recombinant ADAMTS in Insect Cellsologies related to increased turnover of the extracellular matrix, particularly in tissues where the concentration of proteoglycans is high, such as cartilage and the central nervous system. We have expressed three of these enzymes, ADAMTS-1, -4 and -5, in insect cells using plasmid-based systems.作者: 谷物 時間: 2025-3-29 17:10
Refolding of TIMP-2 from , Inclusion Bodiesintracellular expression and efficient isolation of the recombinant product without the use of fusion tags or partners. Protein purity after ion exchange and gel filtration chromatography was judged to be greater than 95% with yields of 15?mg/L from LB medium and 10?mg/L from minimal medium.作者: 逗它小傻瓜 時間: 2025-3-29 22:46
Methods for Studying Activation of Matrix Metalloproteinasesrerequisite for understanding MMP-mediated proteolysis..The emphasis of this chapter is the provision of the experimental tools to study MMP activation in vitro and in cellular model systems. Hence, we use the activation of procollagenase-3 (proMMP-13) and progelatinase A (proMMP-2) as examples of the methods used.作者: paroxysm 時間: 2025-3-30 00:47 作者: contrast-medium 時間: 2025-3-30 06:20
Analysis of the Degradome with the CLIP-CHIP? Microarraych parts of the sequence of known or predicted protease and inhibitor mRNAs in both species and printed on a glass-matrix surface, the CLIP-CHIP? microarray can be used to analyze differentially expressed protease and inhibitor gene products and give expression profiles for any analyzed sample.作者: Ingrained 時間: 2025-3-30 11:41
Mouse Models of MMP and TIMP Functiondiverse and, at times, unexpected functions of MMPs largely arose from the use of gene-targeted mice. In this chapter, we discuss the phenotypes of some MMP-deficient and TIMP-null mice and strategies and pitfalls in targeted mutagenesis.作者: pancreas 時間: 2025-3-30 15:35
1064-3745 n, purification, detection, and assay of MMPs and TIMPs.Deta.Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse orga作者: Instinctive 時間: 2025-3-30 18:20 作者: Ovulation 時間: 2025-3-30 23:30
Ian M. ClarkIncludes two comprehensive review chapters and twenty-five detailed method chapters.Provides state-of-the-art methodologies.Covers expression, purification, detection, and assay of MMPs and TIMPs.Deta作者: hair-bulb 時間: 2025-3-31 04:11
Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/627759.jpg作者: 有抱負(fù)者 時間: 2025-3-31 07:08 作者: 圍裙 時間: 2025-3-31 11:28
https://doi.org/10.1007/978-1-60327-299-5Escherichia coli; Microarray; Nucleotide; PCR; Proteomics; Single Nucleotide Polymorphism; enzymes; hybridi作者: 獨白 時間: 2025-3-31 14:40
