標(biāo)題: Titlebook: Mass Spectrometry-Based Proteomics; Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr [打印本頁] 作者: Asphyxia 時(shí)間: 2025-3-21 19:31
書目名稱Mass Spectrometry-Based Proteomics影響因子(影響力)
作者: 蹣跚 時(shí)間: 2025-3-21 22:56 作者: 大方不好 時(shí)間: 2025-3-22 01:55
Sensitive and High-Throughput Exploration of Protein N-Termini by TMT-TAILS N-Terminomics,vestigation of differential proteolysis, protease substrate discovery, and analysis of N-terminal PTMs. In this chapter, we provide a state-of-the-art protocol, with detailed steps in all parts of the TAILS sample preparation, MS analysis, and post-processing of acquired data.作者: 小臼 時(shí)間: 2025-3-22 07:33 作者: 驕傲 時(shí)間: 2025-3-22 09:54
Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry,pter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information.作者: 橢圓 時(shí)間: 2025-3-22 16:08
Book 2023 surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosph作者: Medley 時(shí)間: 2025-3-22 18:16 作者: CHAR 時(shí)間: 2025-3-22 23:55
Density-Based Fractionation of Cell-Conditioned Medium to Prepare Proteomics Grade Extracellular Vecentrifugation and size-exclusion chromatography to prepare EV from cell-conditioned medium with high specificity and repeatability. This approach enables the recovery of intact purified EV suited for downstream functional assays and biomarker discovery by omics approaches.作者: escalate 時(shí)間: 2025-3-23 04:40 作者: 運(yùn)氣 時(shí)間: 2025-3-23 06:06
Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States, experiments using live cells or crude cell extracts. We provide the tools to perform different TPP formats, i.e., temperature range experiments, TPP-TR; isothermal compound titrations, TPP-CCR; and a combination thereof, 2D-TPP.作者: 暫時(shí)過來 時(shí)間: 2025-3-23 12:25 作者: gene-therapy 時(shí)間: 2025-3-23 15:10
Automated Sample Preparation for Mass Spectrometry-Based Clinical Proteomics,roteolysis. It enables the concurrent processing of 96 clinical samples of any type (fresh-frozen or FFPE tissue, liquid biopsies, or cells) on an automated liquid handling platform, which can be directly interfaced to LC–MS for proteome analysis of clinical specimens with high sensitivity, high reproducibility, and short turn-around times.作者: adroit 時(shí)間: 2025-3-23 20:03
Phosphoproteomics After Guanidinium Thiocyanate Extraction of Tissue Biopsies,phenol-chloroform (AGPC) extraction with RNA-Bee. We describe the necessary steps for biopsy collection, cryoprocessing, and protein extraction. We further share our practice on protein digestion and cleanup of small samples (200?μg protein) and describe settings for automated IMAC-based phosphopeptide enrichment with the AssayMAP Bravo platform.作者: 令人悲傷 時(shí)間: 2025-3-24 01:16 作者: Limerick 時(shí)間: 2025-3-24 03:52 作者: 商談 時(shí)間: 2025-3-24 09:03 作者: inflate 時(shí)間: 2025-3-24 12:28
Jade I. Hawksworth,Lode Denolf,Evy Timmerman,Kris Gevaert作者: concubine 時(shí)間: 2025-3-24 17:34
Esperanza Fernández,Laia Miret-Casals,Annemieke Madder,Kris Gevaert作者: Neuropeptides 時(shí)間: 2025-3-24 22:35
Reuben Samson,Francesco Zangari,Anne-Claude Gingras作者: GREG 時(shí)間: 2025-3-25 01:11
George D. Moschonas,Margaux De Meyer,Delphine De Sutter,Evy Timmerman,Petra Van Damme,Sven Eyckerman作者: Cubicle 時(shí)間: 2025-3-25 04:18 作者: 剝皮 時(shí)間: 2025-3-25 10:45
Konstantinos Kalogeropoulos,Louise Bundgaard,Ulrich auf dem Keller作者: altruism 時(shí)間: 2025-3-25 12:53
Thierry Meinnel,Jean-Baptiste Boyer,Carmela Giglione作者: 躺下殘殺 時(shí)間: 2025-3-25 19:51
Kathirvel Alagesan,Emmanuelle Charpentierugeben ist. Dabei wird auf die Realisierbarkeit oft zun?chst keine Rücksicht genommen, so da? diese Methode in erster Linie für überschl?gige Betrachtungen geeignet ist. Andererseits lassen sich bei geschickter Wahl der vorausgesetzten Eigenschaften ganze Klassen von Systemen in ihrem prinzipiellen 作者: 核心 時(shí)間: 2025-3-25 23:43
Xiangyu Xu,Kris Gevaert,Ive De Smet,Lam Dai Vuugeben ist. Dabei wird auf die Realisierbarkeit oft zun?chst keine Rücksicht genommen, so da? diese Methode in erster Linie für überschl?gige Betrachtungen geeignet ist. Andererseits lassen sich bei geschickter Wahl der vorausgesetzten Eigenschaften ganze Klassen von Systemen in ihrem prinzipiellen 作者: 死亡率 時(shí)間: 2025-3-26 02:47 作者: 啞劇 時(shí)間: 2025-3-26 05:00 作者: 谷類 時(shí)間: 2025-3-26 10:17 作者: PTCA635 時(shí)間: 2025-3-26 16:27
Quentin Roux,Sarah Deville,An Hendrixeitet (“Matched Filter”); anschlie?end erfolgt die geschlossene Berechnung der Bitfehlerwahrscheinlichkeit bei gau?verteiltem Kanalrauschen. Die Ergebnisse werden am Beispiel der PCM-Ubertragung veranschaulicht. Durch lineare Verzerrungen auf dem übertragungswege wird unter praktischen Bedingungen d作者: diathermy 時(shí)間: 2025-3-26 17:10
Roberta Noberini,Tiziana Bonaldi in Abschnitt 4.3 behandelt wird. Sie basiert auf dem Prinzip der linearen Pr?diktion, der in diesem Zusammenhang einige Aufmerksamkeit zu widmen ist. Als Sendestrukturen für die Erzeugung von differentiell pulscodemodulierten Signalen ist zwischen Vorw?rts- und Rückw?rts-Pr?diktion zu unterscheiden作者: podiatrist 時(shí)間: 2025-3-27 00:38 作者: JIBE 時(shí)間: 2025-3-27 03:04 作者: 團(tuán)結(jié) 時(shí)間: 2025-3-27 08:06
tion erl?utert. Zun?chst wird auf einen günstigen Entwurf von Zwischenfrequenzfiltern in Hinblick auf minimale Verzerrungen eingegangen. Dabei werden digitale nichtrekursive Filter zugrunde gelegt, die streng linearphasig entworfen werden k?nnen und somit keinerlei Phasenverzerrungen einbringen. Im 作者: Initial 時(shí)間: 2025-3-27 10:40
Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming,nducible lentiviral toolkit for BioID, we define here protocols for time-resolved PDB in primary cells. Here, we report the optimization and application of lentivirally delivered miniTurbo constructs to a mouse fibroblast model of somatic cell reprogramming, allowing the study of this dynamic proces作者: 睨視 時(shí)間: 2025-3-27 17:24
Virotrap: Trapping Protein Complexes in Virus-Like Particles,and biotin-dependent identification (BioID), Virotrap was shown to complement data obtained with the existing MS-based toolkit. The proven complementarity of these MS-based strategies underlines the importance of using different techniques to enable comprehensive mapping of protein-protein interacti作者: 填料 時(shí)間: 2025-3-27 20:01
Systems-Wide Site-Specific Analysis of Glycoproteins,description of the methods that include procedures for (i) proteolytic digestion using specific proteases, (ii) optional glycopeptide enrichment using hydrophilic interaction liquid chromatography, (iii) nano-LC-MS/MS analysis of glycopeptides, and (iv) data analysis for identification of glycopepti作者: CHASE 時(shí)間: 2025-3-27 22:34 作者: AGONY 時(shí)間: 2025-3-28 03:20
Book 2023sts of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Mass Spectrometry-Based Proteomics. serves as an ideal guide to its subject for both novices in the field o作者: 石墨 時(shí)間: 2025-3-28 07:48 作者: glisten 時(shí)間: 2025-3-28 11:44 作者: impale 時(shí)間: 2025-3-28 18:21
Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming, the mechanistic underpinning of morphological and fate changes associated with these processes. The advent of proximity-dependent biotinylation (PDB) methods has overcome?some of?the limitations of biochemical purification methods, enabling proteomic characterization of most subcellular compartment作者: 混合,攙雜 時(shí)間: 2025-3-28 20:26 作者: 聚集 時(shí)間: 2025-3-29 01:37 作者: PHON 時(shí)間: 2025-3-29 05:52 作者: 相互影響 時(shí)間: 2025-3-29 09:34
Sensitive and High-Throughput Exploration of Protein N-Termini by TMT-TAILS N-Terminomics,the characterization of physiological or proteolytically processed protein N-termini, as well as other N-terminal posttranslational modifications (PTMs). TAILS is a well-established, high-throughput, negative enrichment workflow that enables system-wide exploration of N-terminomes independent of sam作者: 不給啤 時(shí)間: 2025-3-29 12:08
The Global Acetylation Profiling Pipeline for Quick Assessment of Protein N-Acetyltransferase Speciable sensitivity and robustness of the GAP pipeline relies on the very low frequency of known N-terminal acetylated proteins in ., including their degree of N-terminal acetylation. Using the SILProNAQ mass spectrometry strategy on bacterial protein extracts, GAP permits easy acquisition of both qual作者: impale 時(shí)間: 2025-3-29 16:57 作者: neutralize 時(shí)間: 2025-3-29 21:50 作者: 農(nóng)學(xué) 時(shí)間: 2025-3-30 00:19 作者: OASIS 時(shí)間: 2025-3-30 04:59
Proteomics-Based Analysis and Diagnosis of Formalin-Fixed Paraffin-Embedded Amyloidosis Samples,f amyloidosis often relies on immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) patient samples; however, dependency on antibodies for protein staining is one of the major pitfalls of this approach, especially for the detection of rare amyloidosis types. In recent years, mass spectrome作者: carotenoids 時(shí)間: 2025-3-30 10:16
A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isto-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC–MS/MS-based proteomics. As an 作者: monochromatic 時(shí)間: 2025-3-30 12:34 作者: 抓住他投降 時(shí)間: 2025-3-30 16:38
Mass Spectrometry-Based Analysis of Histone Posttranslational Modifications from Laser Microdissectout the implication of epigenetics in a multitude of physiological and pathological processes. Mass spectrometry (MS) has emerged as the most accurate and versatile tool to detect and quantify histone PTMs and has also been applied to clinical specimens, thanks to protocols developed during the past作者: 迅速成長 時(shí)間: 2025-3-30 22:23 作者: grandiose 時(shí)間: 2025-3-31 01:34
Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry,a protein structure. It has been widely recognized as an indispensable tool and already approved by several regulatory agencies as a structural technique for the validation of protein biopharmaceuticals, including antibody-based drugs. Antibodies are of a key importance in life and medical sciences 作者: chalice 時(shí)間: 2025-3-31 07:59 作者: 藐視 時(shí)間: 2025-3-31 13:04 作者: Vital-Signs 時(shí)間: 2025-3-31 16:46 作者: 閑逛 時(shí)間: 2025-3-31 21:27
Delphi Van Haver,Amélie Dendooven,Francis Impensm Fernsprechbereich benutzten Tr?gerfrequenztechnik als ein Beispiel für ein hierarchisches Konzept zur Mehrfachausnutzung von übertragungskan?len. Aufgrund der verh?ltnism??ig geringen qualitativen Ansprüche an die Sprachübertragung wird unter dem Gesichtspunkt einer optimalen Bandbreite-Effizienz 作者: 嘮叨 時(shí)間: 2025-4-1 00:41
Leyla A. Erozenci,Irene V. Bijnsdorp,Sander R. Piersma,Connie R. Jimenezd der Fall unvollst?ndig ged?mpfter benachbarter Sendesignale untersucht, was z.B. bei der Rundfunkübertragung infolge sehr dicht angeordneter Sendefrequenzen ein gro?es Problem darstellt. Die Auswirkungen auf das demodulierte Signal werden für Amplituden- und Frequenzmodulation analysiert. Additive作者: 陪審團(tuán)每個(gè)人 時(shí)間: 2025-4-1 05:38
Quentin Roux,Sarah Deville,An Hendrixen Impulsformungsfiltern unter dem Aspekt der Intersymbol-Interferenz. Diese überlegungen führen auf die erste Nyquist-Bedingung. Die zweite Nyquist-Bedingung berücksichtigt die Beeintr?chtigung einer korrekten Datenentscheidung bei nichtidealer Symbolabtastung am Empf?nger; sie wird in Abschnitt 5.作者: 語言學(xué) 時(shí)間: 2025-4-1 06:23
