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標(biāo)題: Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Jennifer J. Kohler,Steven M. Patrie Book 2013 Springer Science+Business Media, LLC [打印本頁]

作者: hearing-aid    時間: 2025-3-21 16:43
書目名稱Mass Spectrometry of Glycoproteins影響因子(影響力)




書目名稱Mass Spectrometry of Glycoproteins影響因子(影響力)學(xué)科排名




書目名稱Mass Spectrometry of Glycoproteins網(wǎng)絡(luò)公開度




書目名稱Mass Spectrometry of Glycoproteins網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Mass Spectrometry of Glycoproteins被引頻次




書目名稱Mass Spectrometry of Glycoproteins被引頻次學(xué)科排名




書目名稱Mass Spectrometry of Glycoproteins年度引用




書目名稱Mass Spectrometry of Glycoproteins年度引用學(xué)科排名




書目名稱Mass Spectrometry of Glycoproteins讀者反饋




書目名稱Mass Spectrometry of Glycoproteins讀者反饋學(xué)科排名





作者: VERT    時間: 2025-3-21 21:20

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Mass Spectrometry of Glycoproteins978-1-62703-146-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
作者: curettage    時間: 2025-3-22 08:07
Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass finity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis by LC-MS/MS.
作者: 羊齒    時間: 2025-3-22 11:22
In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysisf glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.
作者: Adherent    時間: 2025-3-22 16:30
Jennifer J. Kohler,Steven M. PatrieResource for those who work at the interface of glycobiology and mass spectrometry.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/625277.jpg
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Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptidesthe peptide backbone with intact glycans still attached are used to pinpoint the glycosylation sites of glycopeptides containing several Ser/Thr residues. The step-by-step protocols for fragmentation analyses of O- and N-glycopeptides enriched from human cerebrospinal fluid are described.
作者: 接合    時間: 2025-3-23 01:51
Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Cfor pre-fractionation of complex samples at the protein or peptide level. In this chapter we present a straightforward HILIC SPE enrichment technique and then combine C18 RP and HILIC enrichment for analysis of glycopeptides. Finally, we demonstrate HILIC enrichment using trifluoroacetic acid as an
作者: Constrain    時間: 2025-3-23 07:00
Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization-induced dissociation (CID) at low energies are also described. Ample sections are further devoted to on-line CE/ESI MS technique and its application to separation and identification of O-glycopeptides and CS/DS oligosaccharides. The concept and construction principles of two different sheathless C
作者: sphincter    時間: 2025-3-23 10:49
Book 2013 and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..?.Authoritative and practical, .Mass Spectrometry of Glycoproteins: Methods and Protocol .?is? an essential resource for those who work at the interface of glycobiolog
作者: elucidate    時間: 2025-3-23 16:31
Gerhild Zauner,Carolien A. M. Koeleman,André M. Deelder,Manfred Wuhrerlegenden überblick verschaffen und im Anschluss zum Aufbau eines Nachhaltigkeitsmanagements in Ihrer Organisation des Gesundheitswesens beitragen..978-3-658-44393-1978-3-658-44394-8Series ISSN 2197-6708 Series E-ISSN 2197-6716
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Alina D. Zamfir,Corina Flangea,Alina Serb,Ana-Maria Zagrean,Andreas M. Rizzi,Eugen Sisu
作者: 有常識    時間: 2025-3-24 22:08
Introduction to Glycosylation and Mass Spectrometry,
作者: 不怕任性    時間: 2025-3-25 02:43
Tandem Lectin Weak Affinity Chromatography for Glycoprotein Enrichment
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CSC Technology: Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells
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Nano-HPLC-MS of Glycopeptides Obtained After Nonspecific Proteolysisnalyzed by nano-electrospray ionization multistage mass spectrometry for identification of the glycan as well as the peptide moiety. Using this approach, site-specific information on protein glycosylation is obtained.
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Stable Isotope Labeling of N-Glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometr-mediated .O labeling of .-glycosylated peptides, termed “isotope-coded glycosylation site-specific tagging.” Coupled with advanced mass spectrometry-based proteomics technology, this method facilitates the identification of hundreds to thousands of .-glycoproteins, coupled with their sites of glycosylation, from a complex biological mixture.
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Approaches for Site Mapping and Quantification of O-Linked Glycopeptidesccupancy, structure–function relationships, and the contributions of O-linked glycosylation to physiological and pathological processes. In this chapter, we refer to several approaches for the structural characterization and quantification of O-linked glycopeptides, with a focus on .-GlcNAc and .-Mannose modified glycoproteins.
作者: kindred    時間: 2025-3-26 16:35
Book 20130% of eukaryotic proteins are modified by some type of glycan. In .Mass Spectrometry of Glycoproteins: Methods and Protocols, .expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.? These methods and techniques include robust sample preparation te
作者: 有發(fā)明天才    時間: 2025-3-26 20:14
1064-3745 esults.Contains key notes and implementation advice from the.?Glycosylation is the most abundant post-translational modification of proteins.? Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In .Mass Spectrometry of Gl
作者: 仔細(xì)閱讀    時間: 2025-3-26 23:36
Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass finity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis
作者: 載貨清單    時間: 2025-3-27 02:56

作者: 拒絕    時間: 2025-3-27 05:36
In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysisf glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples
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Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionizatio electrospray ionization (ESI) mass spectrometry (MS), CE enables fast, sensitive, and efficient separations for the accurate identification of a large variety of glycoform mixture types. In this chapter several reliable off- and on-line CE-based methods for the analysis of glycoforms with ESI MS/MS
作者: originality    時間: 2025-3-28 00:48
Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomicsycoconjugates. IM-MS provides separations in two dimensions; one on the basis of molecular surface area or structure, and the other on molecular mass which creates the ability to differentiate biomolecular classes and isobaric species. When applied to the characterization of glycoproteins, IM-MS sep
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作者: 符合你規(guī)定    時間: 2025-3-28 08:39
Stable Isotope Labeling of N-Glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometrl glycosylation is also associated with various pathologies, including cancer, and inflammatory and degenerative diseases, technology for comprehensive analysis of glycoproteins, or glycoproteomics, is important not only for biological studies but also for biomedical and clinical research, including
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Gerhild Zauner,Carolien A. M. Koeleman,André M. Deelder,Manfred WuhrerNachhaltigkeitsberichterstattung.Mit praxisnahen Beispiele uDas Thema Nachhaltigkeitsmanagement gewinnt auch im Gesundheitssektor stark an Bedeutung. Nicht zuletzt, da auf Bundes- und europ?ischer Ebene neue rechtliche Anforderungen gestellt werden, die auch für viele Organisationen des Gesundheitsw
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re’s engagement and use of the past.Includes essays from lea.This book takes an interdisciplinary approach to understanding the different ways in which the past remains present in Western popular culture in the twenty-first century. It combines theoretical analyses with case study-based chapters foc
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Zurich Around 1750: Economic and Cultural Boom and Revolutionary Activities,eadfast in the face of the seductions of a capitalized society. This conviction is the key expression of the educational turn—that is, the educationalization of social problems. As a result of these developments and conditions, Zurich experienced an outbreak of youth unrest in which the young Pestalozzi would be socialized after 1760.
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Annemarijke van Luijtelaar,Joyce G. R. Bomers,Jurgen J. Füttererienoptionen den Standard dar. Unter den numerischen Ans?tzen gilt das Binomialmodell als wichtiger Ansatz. Beide Theorien sollen im vorliegenden Kapitel als Standardmodelle der Optionsbewertung detailliert ausgeführt werden. Zur Vereinfachung wird dabei ausschlie?lich der Fall der Optionen auf Aktie
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Reyna Favisin Molecular Biology? series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and 978-1-4939-5700-2978-1-60761-744-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
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The Gaussian Approximation Potential978-3-642-14067-9Series ISSN 2190-5053 Series E-ISSN 2190-5061
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