標(biāo)題: Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Arnaud Delobel Book 2021 Springer Science+Business Media, LLC, part of Springer Na [打印本頁] 作者: incoherent 時(shí)間: 2025-3-21 16:25
書目名稱Mass Spectrometry of Glycoproteins影響因子(影響力)
書目名稱Mass Spectrometry of Glycoproteins影響因子(影響力)學(xué)科排名
書目名稱Mass Spectrometry of Glycoproteins網(wǎng)絡(luò)公開度
書目名稱Mass Spectrometry of Glycoproteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Mass Spectrometry of Glycoproteins被引頻次
書目名稱Mass Spectrometry of Glycoproteins被引頻次學(xué)科排名
書目名稱Mass Spectrometry of Glycoproteins年度引用
書目名稱Mass Spectrometry of Glycoproteins年度引用學(xué)科排名
書目名稱Mass Spectrometry of Glycoproteins讀者反饋
書目名稱Mass Spectrometry of Glycoproteins讀者反饋學(xué)科排名
作者: nauseate 時(shí)間: 2025-3-21 22:24
Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Massr the reader a practical overview of the available methodologies for the confident analysis of intact glycoproteins with different glycosylation degree by MALDI-TOF MS. The three proposed methods fulfil the requirements of reproducibility and low extent of glycan fragmentation required to successfully analyze intact glycoproteins.作者: 我沒有強(qiáng)迫 時(shí)間: 2025-3-22 01:15 作者: Provenance 時(shí)間: 2025-3-22 06:35
Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS,mide bonded stationary phases packed with sub-2?μm particles has allowed for remarkable separations also at the subunit level. Here, we describe a simple protocol to perform the mAb glycans profiling at subunit level by HILIC/MS.作者: offense 時(shí)間: 2025-3-22 09:23
,Site-Specific O-Glycosylation Analysis by Liquid Chromatography–Mass Spectrometry with Electron-Tranalysis during the development of therapeutic glycoproteins. Here, we describe a method of in-depth site-specific O-glycosylation analysis by liquid chromatography–mass spectrometry using electron-transfer/higher-energy collisional dissociation (EThcD) and database analysis.作者: electrolyte 時(shí)間: 2025-3-22 16:04
Routine Analysis of ,-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection,l-enhancement label, LC-MS data generation, and data analysis. Using this method, up to 24 .-glycan samples can be prepared at one time and analyzed by LC-MS. With the addition of automation platform, up to 96 .-glycan samples can be prepared and analyzed in a high-throughput manner.作者: 浪蕩子 時(shí)間: 2025-3-22 18:59 作者: Visual-Field 時(shí)間: 2025-3-22 22:53 作者: flavonoids 時(shí)間: 2025-3-23 03:16 作者: 比目魚 時(shí)間: 2025-3-23 07:16 作者: Atmosphere 時(shí)間: 2025-3-23 12:47
Use of Exoglycosidases for the Structural Characterization of Glycans,ct labeling of released glycans with procainamide (PCA) or 2-aminobenzamide (2AB), cleanup of labeled glycans and a 3?h enzymatic digestion with exoglycosidases. This protocol is designed for completion within an 8?h time frame to allow for subsequent LC-FLD, LC-MS, or CE analysis overnight.作者: 演繹 時(shí)間: 2025-3-23 17:29
Book 2021sses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact p作者: SNEER 時(shí)間: 2025-3-23 21:41
1064-3745 ation advice from the expertsThis volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in thi作者: Absenteeism 時(shí)間: 2025-3-23 22:15 作者: PTCA635 時(shí)間: 2025-3-24 02:20
Mapping ,-glycosylation Sites Using OpeRATOR and LC-MS,s enables the generation of glycopeptides of suitable size for mapping .-glycosylation sites in detail by bottom-up LC-MS analysis. In this chapter we demonstrate a simple workflow for in-depth analysis of .-glycosylation sites on heavily glycosylated proteins using OpeRATOR digestion and HILIC-MS/MS analysis.作者: Rodent 時(shí)間: 2025-3-24 06:34 作者: BILE 時(shí)間: 2025-3-24 12:32
Profiling, Relative Quantification, and Identification of Sialylated ,-Linked Oligosaccharides by Uarge and the sialylation level is determined based on the fluorescence signal. In addition, in this method, the .-glycans are also separated by type and identified with the hyphenated MS. For both methods, an optimized protocol with fast and high-throughput sample preparation and purification is presented.作者: BROOK 時(shí)間: 2025-3-24 16:48
1064-3745 known pitfalls..Cutting-edge and thorough, .Mass Spectrometry of Glycoproteins: Methods and Protocols. is a valuable resource for scientists interested in learning more about this978-1-0716-1243-9978-1-0716-1241-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: LAST 時(shí)間: 2025-3-24 22:44 作者: 治愈 時(shí)間: 2025-3-25 01:37
Yosra Helali,Shilpee Sharma,Marie Vandeput,Dansala Welba,Pierre Van Antwerpen,Arnaud Marchant,Cédric作者: neutralize 時(shí)間: 2025-3-25 06:35
Elsa Wagner-Rousset,Olivier Colas,Stéphane Chenu,Yannis-Nicolas Fran?ois,Davy Guillarme,Sarah Cianfe作者: Acclaim 時(shí)間: 2025-3-25 08:05
Josiane Saadé,Michael Biacchi,Jérémie Giorgetti,Antony Lechner,Alain Beck,Emmanuelle Leize-Wagner,Ya作者: 意外 時(shí)間: 2025-3-25 13:43
Maria Nordgren,Andreas N?geli,Helén Nyhlén,Jonathan Sj?gren作者: CORD 時(shí)間: 2025-3-25 18:56
Ian Black,Christian Heiss,Russell W. Carlson,Parastoo Azadi作者: obligation 時(shí)間: 2025-3-25 22:20
Dan Bach Kristensen,Trine Meiborg Sloth,Martin ?rgaard,Pernille Foged Jensenwird, entsteht ein neues Optimierungskalkül für Unternehmensleitung und Aktion?re. Um dabei fundierte Entscheidungen treffen zu k?nnen, müssen jedoch Informationen publiziert werden. Die Untersuchung ergründet, inwieweit die aktuelle Nachhaltigkeitsberichterstattungspraxis in Deutschland kompatibel 作者: 莊嚴(yán) 時(shí)間: 2025-3-26 02:13 作者: NATTY 時(shí)間: 2025-3-26 07:54 作者: 聲明 時(shí)間: 2025-3-26 08:56 作者: 驚奇 時(shí)間: 2025-3-26 13:54
Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS,nsitive and reproducible analytical approach..This chapter describes an optimized approach for the preparation and the analysis of Fc .-glycans from total serum or plasma IgG using magnetic beads, RapiFluor MS label?, and LC-MS.作者: 贊成你 時(shí)間: 2025-3-26 17:37
Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophiction chromatography (SAX-ERLIC). Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis. Glycopeptide data generated using the SAX-ERLIC enrichment yields a high numb作者: Colonnade 時(shí)間: 2025-3-27 01:01 作者: 口訣 時(shí)間: 2025-3-27 04:33 作者: 不愿 時(shí)間: 2025-3-27 07:46
Book 2021aterials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Mass Spectrometry of Glycoproteins: Methods and Protocols. is a valuable resource for scientists interested in learning more about this作者: Reservation 時(shí)間: 2025-3-27 11:14 作者: Glutinous 時(shí)間: 2025-3-27 15:04 作者: Reverie 時(shí)間: 2025-3-27 21:05 作者: JAMB 時(shí)間: 2025-3-27 22:29 作者: 吞下 時(shí)間: 2025-3-28 03:26
Sonja Schneider,Edgar Naegele,Sonja Krieger einzuhalten. Teilweise sind sogar die Besucher der Mieter dazu verpflichtet. Die Hausordnung hat einen Appellcharakter. Ihre Verbindlichkeit erh?ht sich, wenn sie Teil des Mietvertrages ist und von beiden Seiten unterzeichnet wird. Im Vergleich zum gewerblichen Mietvertrag, gibt es viele Hürden, di作者: 傻 時(shí)間: 2025-3-28 07:34
Claire I. Butré,Eric Largy,Fabrice Cantais,Arnaud Delobel veranlassen immer mehr Mitarbeiter zum Wegzug aus den St?dten. Das Pendeln zur Arbeit zur Norm. Als Autofahrernation nutzten im Jahr 2022 rund 68?% der Pendler das Auto. Rund die H?lfte der Pendler haben einen Fahrtweg von unter 10 km zur Arbeitsst?tte zu bew?ltigen. Der durchschnittliche CO.-Ausst作者: BLOT 時(shí)間: 2025-3-28 10:28
Glycosylation of Therapeutic Proteins: A Critical Quality Attribute,e host cell line or the culture conditions. N- and O-linked glycans usually play a great role on the stability, safety, and efficacy of the drug. For this reason, glycosylation is considered as a critical quality attribute of therapeutic glycoproteins, and a thorough characterization should be perfo作者: 爆炸 時(shí)間: 2025-3-28 18:28 作者: Aids209 時(shí)間: 2025-3-28 19:54 作者: FLEET 時(shí)間: 2025-3-29 00:21 作者: mortuary 時(shí)間: 2025-3-29 04:41
Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry,tative glycoengineered monoclonal antibodies (mAbs), namely, obinutuzumab (GlycomAb technology, Glycart/Roche), benralizumab (Potelligent Technology, BioWa, Kyowa Kirin) and mAb B (kifunensine) and compared to mAb A, produced in a common CHO cell line. In addition, EndoS or EndoS2 enzyme are used fo作者: 單片眼鏡 時(shí)間: 2025-3-29 09:18 作者: stress-test 時(shí)間: 2025-3-29 12:39 作者: fodlder 時(shí)間: 2025-3-29 16:57
Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophi and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest. In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and compositio作者: 點(diǎn)燃 時(shí)間: 2025-3-29 21:40
In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC SPE and LC-MS/MS,subtle changes in the cellular environment. Therefore, comprehensive and in-depth glycan characterization of therapeutic glycoproteins should be performed to ensure product quality and process consistency, but it is analytically challenging due to glycan microheterogeneity occurring in the glycan bi作者: 是比賽 時(shí)間: 2025-3-30 03:01
Site-Specific ,-glycosylation Analysis of Recombinant Proteins by LC/MS,context of recombinant glycoproteins, glycosylation is a critical attribute as glycans could dramatically alter protein functions and properties including activity, half-life, in vivo localization, stability, and, last but not least, immunogenicity. Liquid chromatography combined to mass spectrometr作者: 音樂學(xué)者 時(shí)間: 2025-3-30 05:50
Mapping ,-glycosylation Sites Using OpeRATOR and LC-MS,allenging. OpeRATOR is an .-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This enables the generation of glycopeptides of suitable size for mapping .-glycosylation sites in detail by bottom-up LC-MS analysis. In this chapter we作者: lacrimal-gland 時(shí)間: 2025-3-30 09:50 作者: 歡呼 時(shí)間: 2025-3-30 15:41
Profiling of ,-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS After Derivatization wi and type of glycosylation is a characterization parameter for the analysis of antibodies and other therapeutic proteins. The method described here is a fast and high-throughput method for identification and semiquantification of .-glycans by HILIC-FLR-ESI-MS. Sample preparation has been optimized a作者: 豐富 時(shí)間: 2025-3-30 17:09
Evaluating ,-Glycosylation of a Therapeutic Monoclonal Antibody Using UHPLC-FLR-MS with ,Fluor-MS Lveral years. The more recent .Fluor-MS? labeling technique, however, offers enhanced mass spectrometric detection of released .-glycans, improving the sensitivity and detection limits of the method. The optimized multidimensional detection offers increased confidence in glycan identification which c作者: 滲透 時(shí)間: 2025-3-31 00:38 作者: Regurgitation 時(shí)間: 2025-3-31 03:21 作者: 江湖騙子 時(shí)間: 2025-3-31 07:35
Profiling, Relative Quantification, and Identification of Sialylated ,-Linked Oligosaccharides by U the immunogenicity properties and structural properties of the proteins. Profiling of the .-glycans and their charge state is a requisite for complete protein characterization. Two analytical methods developed on released .-glycans are described in this chapter, allowing for the determination of th作者: Thrombolysis 時(shí)間: 2025-3-31 12:40
Linkage Analysis of Oligosaccharides and Polysaccharides: A Tutorial,rs is vast, ranging from small oligosaccharides of defined composition decorating proteins, to large, complex heteropolymers comprising integral cell wall components of plants, fungi and bacteria. An important step in the elucidation of unknown carbohydrate structures in a sample is the assessment o作者: catagen 時(shí)間: 2025-3-31 13:57 作者: 河流 時(shí)間: 2025-3-31 20:07
Arnaud DelobelIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Optimum 時(shí)間: 2025-3-31 21:46 作者: 慢跑鞋 時(shí)間: 2025-4-1 03:50 作者: CUMB 時(shí)間: 2025-4-1 05:59 作者: 氣候 時(shí)間: 2025-4-1 12:09 作者: 入會(huì) 時(shí)間: 2025-4-1 14:37
The Educationalization of Social Problems Around 1800,h century. Today, it continues unabated and finds expression in the framework of the World Bank, the United Nations, UNESCO, and the OECD. It is based on the premise that the central problems of the present and planning for the future are educational concerns.作者: 無目標(biāo) 時(shí)間: 2025-4-1 19:33
https://doi.org/10.1007/978-3-662-68362-0deeper cavities of the NARSs as compared to the corresponding resorcinarenes are suitable for a variety of neutral guests and anions. Quite interestingly, the four spatially-fixed halides in NARXs are also suitable halogen-bond acceptors leading to multiple assemblies such as deep cavity cavitands, 作者: keloid 時(shí)間: 2025-4-2 01:11
Gordon Guoienoptionen den Standard dar. Unter den numerischen Ans?tzen gilt das Binomialmodell als wichtiger Ansatz. Beide Theorien sollen im vorliegenden Kapitel als Standardmodelle der Optionsbewertung detailliert ausgeführt werden. Zur Vereinfachung wird dabei ausschlie?lich der Fall der Optionen auf Aktie作者: 使?jié)M足 時(shí)間: 2025-4-2 04:45 作者: Modify 時(shí)間: 2025-4-2 07:26 作者: MAZE 時(shí)間: 2025-4-2 14:20
Ann Marie BissessarExamines the dynamics of post-colonial societies and their diverse population.Imposes a new area of discussion on ethnicity and conflict.Provides comparative analysis among Trinidad and Tobago, Guyana作者: 可耕種 時(shí)間: 2025-4-2 18:53
https://doi.org/10.1007/978-1-349-04920-2Asia; political science; politics; system作者: Accomplish 時(shí)間: 2025-4-2 23:30
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