標題: Titlebook: Mass Cytometry; Methods and Protocol Helen M. McGuire,Thomas M. Ashhurst Book 2019 Springer Science+Business Media, LLC, part of Springer N [打印本頁] 作者: 味覺沒有 時間: 2025-3-21 17:19
書目名稱Mass Cytometry影響因子(影響力)
書目名稱Mass Cytometry影響因子(影響力)學科排名
書目名稱Mass Cytometry網(wǎng)絡公開度
書目名稱Mass Cytometry網(wǎng)絡公開度學科排名
書目名稱Mass Cytometry被引頻次
書目名稱Mass Cytometry被引頻次學科排名
書目名稱Mass Cytometry年度引用
書目名稱Mass Cytometry年度引用學科排名
書目名稱Mass Cytometry讀者反饋
書目名稱Mass Cytometry讀者反饋學科排名
作者: 不可接觸 時間: 2025-3-22 00:10 作者: FIN 時間: 2025-3-22 04:18
1064-3745 eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Mass Cytometry: Methods and Protocols. aims to ensure successful results in the further study of this vital field..978-1-4939-9454-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 多嘴多舌 時間: 2025-3-22 07:04 作者: Detonate 時間: 2025-3-22 11:52
Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for ctrophotometry and frequently exceeds 60%. Each custom-conjugated antibody is validated using positive and negative cellular control populations and is titrated for optimal staining at concentrations ranging from 0.1 to 10?μg/mL. The preparation of metal-labeled antibodies can be completed in 4.5?h, and titration requires an additional 3–5?h.作者: Tinea-Capitis 時間: 2025-3-22 13:18 作者: fabricate 時間: 2025-3-22 19:08
Mass Cytometric Cell Cycle Analysisration of 5-Iodo-2′-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), Cyclin B1, and phosphorylated Histone H3 (pHH3). These measurements can be integrated into a gating strategy that enables clear separation of all five phases of the cell cycle.作者: 金桌活畫面 時間: 2025-3-22 22:46 作者: TOXIN 時間: 2025-3-23 05:20 作者: cornucopia 時間: 2025-3-23 09:03
Acquisition, Processing, and Quality Control of Mass Cytometry Data cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.作者: Choreography 時間: 2025-3-23 13:31 作者: 連接 時間: 2025-3-23 16:49
Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometryse, while retaining the possibility to deliver an in-depth characterization of antigen-specific CD8. T cells and associated phenotypes. Here we describe the method for a MHC class I tetramer multiplexing approach together with intracellular antibody staining for a parallel phenotypic cell characterization using mass cytometry in human specimens.作者: Lament 時間: 2025-3-23 20:24
Book 2019y reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Mass Cytometry: Methods and Protocols. aims to ensure successful results in the further study of this vital field..作者: 盡管 時間: 2025-3-23 22:37
1064-3745 ation advice from the experts.This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly succ作者: 翻動 時間: 2025-3-24 06:03
Book 2019 design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readil作者: evaculate 時間: 2025-3-24 07:43
Diana Shinko,Thomas M. Ashhurst,Helen M. McGuire,Kellie A. Charles作者: 夾死提手勢 時間: 2025-3-24 14:36
Automated Cell Processing for Mass Cytometry Experimentsy automated using a liquid handling robotic system and we present measures taken to optimize all steps of the protocol. These advances are applicable to both manual and automated protocols and provide a six-fold higher cell yield as compared to a standard protocol. With this increased yield and impr作者: narcotic 時間: 2025-3-24 16:53
Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometryic system in the bone marrow (BM) is a critical component of both immunity and disease. Because of the complexity of the hematopoietic system in steady state and disease, high-dimensional cytometry technologies are well suited to the exploration of these complex systems. Here we describe a protocol 作者: 閑蕩 時間: 2025-3-24 21:55
Yannick Simoni,Michael Fehlings,Evan W. Newell the nuclei 19F and 15N, subvolume III/35C contains the nucleus 1H, subvolume III/35D contains the nucleus 13C, subvolume III/35E contains the nucleus 17O, and subvolume III/35G contains the nucleus 77Se. More nuclei will be presented later..978-3-540-74189-3Series ISSN 1615-1844 Series E-ISSN 1616-9522 作者: 薄荷醇 時間: 2025-3-25 00:04 作者: 無禮回復 時間: 2025-3-25 04:56
Gregory K. Behbehani the nuclei 19F and 15N, subvolume III/35C contains the nucleus 1H, subvolume III/35D contains the nucleus 13C, subvolume III/35E contains the nucleus 17O, and subvolume III/35G contains the nucleus 77Se. More nuclei will be presented later..978-3-540-74189-3Series ISSN 1615-1844 Series E-ISSN 1616-9522 作者: HEPA-filter 時間: 2025-3-25 08:46 作者: SLAG 時間: 2025-3-25 12:51 作者: ABYSS 時間: 2025-3-25 16:17 作者: 預兆好 時間: 2025-3-25 21:49 作者: Provenance 時間: 2025-3-26 03:29
Sarah Warth,Désirée Kunkelental expression, and possess a wide range of functional and pharmacological properties. The diversity in subunit composition creates NMDA receptor subtypes with distinct physiological roles across neuronal cell types and brain regions, and enables precise tuning of synaptic transmission. Here, we w作者: Synovial-Fluid 時間: 2025-3-26 06:13
Brian H. Lee,Adeeb H. Rahmanologous expression systems. The problem is that co-expression of GluN1 with two different GluN2 subunits generates two distinct diheteromeric receptor subtypes as well as one triheteromeric receptor subtype, thereby confounding studies on a homogenous population of triheteromeric NMDA receptors. Her作者: 我沒有強迫 時間: 2025-3-26 11:49
ed gene expression and protein biochemistry to examine complex formation between GluN1 subunit (encoded by .) and GluN2A subunit (encoded by .), anchorage-independent growth in soft agar and cellular migration. Furthermore, we used shRNA depletion of endogenous . in melanoma cells expressing either 作者: 打折 時間: 2025-3-26 16:43 作者: 競選運動 時間: 2025-3-26 20:34 作者: TEM 時間: 2025-3-26 22:47 作者: hedonic 時間: 2025-3-27 01:30 作者: 讓空氣進入 時間: 2025-3-27 09:13 作者: 連系 時間: 2025-3-27 11:47
comparisons of the experimental signal matrix with a series (or systematically sampled set) of model coordinates corresponding to a dynamics’ course, driven-minimization or torsional grid search. These procedures and developments are illustrated with examples including: solution conformations of pr作者: Coronary-Spasm 時間: 2025-3-27 16:41
Jaromir Mikes,Axel Olin,Tadepally Lakshmikanth,Yang Chen,Petter Brodintructures. An index of molecular formulas and a shift index, which should prove useful in hypothesizing alternate structures for the unknown and in checking out the hypotheses, have also been provided. The data collected represent some 4800 shifts from the spectra of about 1200 compounds that appear in the ca978-1-4684-7298-1978-1-4684-7296-7作者: 出價 時間: 2025-3-27 20:03
Lisa E. Wagar An index of molecular formulas and a shift index, which should prove useful in hypothesizing alternate structures for the unknown and in checking out the hypotheses, have also been provided. The data collected represent some 4800 shifts from the spectra of about 1200 compounds that appear in the ca作者: 出汗 時間: 2025-3-28 01:30 作者: 信條 時間: 2025-3-28 04:39 作者: infringe 時間: 2025-3-28 08:00
Acquisition, Processing, and Quality Control of Mass Cytometry Datal level. Using isotopically conjugated antibodies, mass cytometry overcomes the limitations of spectral overlap associated with flow cytometry and allows for deeper single cell characterization of complex biospecimens using more cellular markers. However, the nature of mass spectrometry-based single作者: 側面左右 時間: 2025-3-28 13:47
Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Sig sensitivity. Here we describe a procedure for evaluating signal interference dynamics in the context of a 25-parameter core immunophenotyping panel. Specifically, a mass-minus-many (MMM) approach was used to assess background signals in “empty” or “blank” channels intended for further customization作者: Contracture 時間: 2025-3-28 18:02
Method for Tagging Antibodies with Metals for Mass Cytometry Experimentsodies (Abs) or other cellular probes within single cell suspensions or laser-ablated tissue sections. While many strategies exist for covalently crosslinking to proteins, the Fluidigm MaxPar. process is currently the most widely used and involves first loading a metal-chelating polymer with an eleme作者: Popcorn 時間: 2025-3-28 19:14
Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for mited number of metal-labeled antibodies are commercially available. Here we present optimized and scalable protocols for conjugation of lanthanide as well as bismuth ions to immunoglobulin (Ig) using a maleimide-functionalized chelating polymer and for characterization of the conjugate. The maleimi作者: 文藝 時間: 2025-3-28 23:49 作者: 感染 時間: 2025-3-29 03:19
Surface Barcoding of Live PBMC for Multiplexed Mass Cytometryset of individual samples can be pooled and further processed and acquired as a large, single sample. For assays that require uncompromised profiling of cell-surface markers on live cells, barcoding by metal-labeled antibodies targeting cell-surface epitopes is the barcoding approach of choice. Here作者: 能量守恒 時間: 2025-3-29 10:36
Automated Cell Processing for Mass Cytometry Experimentsprobes are coupled to elemental tags, each with a unique mass and detectable at single-cell resolution using an ICP-MS type of instrument. Given the sensitivity of the detection system, any free metal ions must be carefully removed through multiple rounds of washing in order to prevent background si作者: monogamy 時間: 2025-3-29 15:26
Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometryh as flow cytometry. Cell stimulation and the harsh conditions required in the later stages of certain sample preparations also contribute to cell loss. Low starting cell numbers are especially susceptible to these effects, potentially limiting the ability to use mass cytometry. Here is presented a 作者: critique 時間: 2025-3-29 17:19 作者: harmony 時間: 2025-3-29 20:30
Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometrytibility complex (MHC) class I tetramers allow for a direct detection of such antigen-specific CD8. T cells. Using mass cytometry together with multiplex MHC class I tetramer staining, we are able to screen more than 1000 different antigen candidates simultaneously across tissues in health and disea作者: 完全 時間: 2025-3-30 01:40
Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometryan organism. Long-lived hematopoietic stem cells give rise to early progenitors with multi-lineage potential that progressively differentiate into lineage-specific progenitors. Following lineage commitment, these progenitors proliferate and expand, before eventually differentiating into their mature作者: radiograph 時間: 2025-3-30 04:10
Mass Cytometric Cell Cycle Analysisevelopmental of multicellular organisms. While measurement of cell cycle state by fluorescent flow cytometry is well established, mass cytometry allows the cell cycle to be measured along with large numbers of other antigens enabling characterization of the complex interactions between the cell cycl作者: DEMN 時間: 2025-3-30 08:50
Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass CytometryC) populations that are heterogeneous both phenotypically and functionally. Previously, we characterized these diverse MPS phenotypes with multi-parametric mass cytometry (CyTOF). In order to expansively characterize monocytes, macrophages, and dendritic cells, a CyTOF panel was designed to measure 作者: 細查 時間: 2025-3-30 13:09 作者: 有限 時間: 2025-3-30 19:37
eptors (iGluRs). Among iGluRs, NMDA receptors (NMDARs) are unique in their ability to pass large, Ca.-rich currents. Importantly, their high Ca. permeability is essential for normal CNS function and is under physiological control. For this reason, the accurate measurement of NMDA receptor Ca. permea作者: 異教徒 時間: 2025-3-31 00:39
A receptor subunits exist (GluN1, GluN2A-D, and GluN3A-B) that assemble into tetrameric receptor subtypes with distinct functional properties and physiological roles. The majority NMDA receptors are composed of two GluN1 and two GluN2 subunits, which can assemble into four diheteromeric receptors su作者: Injunction 時間: 2025-3-31 01:52
Sarah Warth,Désirée Kunkelem (CNS). They are widely distributed at all stages of development and are critically involved in normal brain functions, including neuronal development and synaptic plasticity. NMDA receptors are also implicated in the pathophysiology of numerous neurological and psychiatric disorders, such as isch
