作者: 匯總 時(shí)間: 2025-3-22 00:15
Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems,t. However, the exchange and modification of the coding and/or noncoding elements to analyze their effect on protein function or generating the optimal result can be a tedious and time-consuming process using standard molecular biology cloning methods. To streamline the process to generate defined e作者: Calibrate 時(shí)間: 2025-3-22 00:25 作者: 最高點(diǎn) 時(shí)間: 2025-3-22 07:03
,A Mammalian-Based Synthetic Biology Toolbox to Engineer Membrane–Membrane Interfaces,s cellular processes including cell signaling, development, and immunity. Using synthetic biology to engineer or to create novel cellular functions in the intercellular regions has led to an increasing need for a platform that allows generation of functionalized intercellular membrane–membrane inter作者: IOTA 時(shí)間: 2025-3-22 08:46 作者: harangue 時(shí)間: 2025-3-22 15:29 作者: 抗體 時(shí)間: 2025-3-22 21:04 作者: Jogging 時(shí)間: 2025-3-22 22:34 作者: 維持 時(shí)間: 2025-3-23 01:48 作者: 符合規(guī)定 時(shí)間: 2025-3-23 07:13
Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Cncoding thousands of unique protein variants. Since plasmid transfection and lentiviral transduction have characteristics that limit multiplexing with pooled libraries, we developed a mammalian synthetic biology platform that harnesses the Bxb1 bacteriophage DNA recombinase to insert single promoter作者: 膽小懦夫 時(shí)間: 2025-3-23 11:53 作者: 銀版照相 時(shí)間: 2025-3-23 13:51 作者: 書法 時(shí)間: 2025-3-23 18:24 作者: ANTIC 時(shí)間: 2025-3-24 00:49
Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems,robial innate immune system, which emerged from the evolutionary arms race between microbes and phages. This coevolution has also given rise to so-called anti-CRISPR (Acr) proteins that counteract the CRISPR-Cas adaptive immunity. Acrs constitutively block cognate CRISPR-Cas effectors, e.g., by inte作者: 鐵砧 時(shí)間: 2025-3-24 05:21 作者: 真 時(shí)間: 2025-3-24 06:37 作者: 邪惡的你 時(shí)間: 2025-3-24 11:12
Imaging ,-Adenosyl Methionine Dynamics in Living Cells Using an RNA-Based Fluorescent Sensor, Understanding the spatiotemporal dynamics of SAM within living cells is essential for deciphering its roles in maintaining cell homeostasis and in disease development. Here, we describe a protocol based on a recently reported SAM sensor exploiting a fluorogenic RNA and an RNA three-way junction for作者: canonical 時(shí)間: 2025-3-24 18:40
High-Throughput Spectroscopic Analysis of mRNA Capping Level, translation initiation, and stability. Here, we describe a novel biosensor method designed to detect the presence of both cap structures and polyadenylation sites on mRNA molecules. This novel biosensor is sensitive to mRNA degradation and can quantitatively determine capping levels of mRNA molecul作者: 季雨 時(shí)間: 2025-3-24 21:04
In Vitro Generation of Megakaryocytes from Engineered Mouse Embryonic Stem Cells,hat can provide insights into the design principles governing cell fate control. We are interested in reprogramming pluripotent stem cells with synthetic gene circuits to drive mouse embryonic stem cells (mESCs) down the hematopoietic lineage for the production of megakaryocytes, the progenitor cell作者: Paradox 時(shí)間: 2025-3-24 23:54
https://doi.org/10.1007/978-1-0716-3718-0Cloning toolkits; Gene expression control; Synthetic epigenetics; CRISPR-based systems; Bioprocessing ap作者: Instrumental 時(shí)間: 2025-3-25 05:18 作者: 駕駛 時(shí)間: 2025-3-25 11:05 作者: famine 時(shí)間: 2025-3-25 13:21 作者: 集中營(yíng) 時(shí)間: 2025-3-25 17:32
978-1-0716-3720-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 宿醉 時(shí)間: 2025-3-25 21:30 作者: –LOUS 時(shí)間: 2025-3-26 03:10 作者: etiquette 時(shí)間: 2025-3-26 07:44
1064-3745 ation advice from the experts.This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mamma作者: Interlocking 時(shí)間: 2025-3-26 10:43 作者: Allodynia 時(shí)間: 2025-3-26 14:06 作者: Somber 時(shí)間: 2025-3-26 20:35 作者: 做作 時(shí)間: 2025-3-27 01:02
High-Throughput Spectroscopic Analysis of mRNA Capping Level,e sensitivity to increases in capping level of at least 20% and a limit of detection of 2.4?pmol of mRNA. Overall, the biosensor can provide key information about mRNA quality before mammalian cell transfection.作者: 預(yù)定 時(shí)間: 2025-3-27 02:00 作者: OTTER 時(shí)間: 2025-3-27 07:20
RNA Switches Using Cas Proteins,nd the available tools in synthetic biology because of the wide range of Cas protein orthologs that can be used as trigger proteins..Here, we describe the design principle of Cas protein-responsive switches, both plasmid and RNA versions, using . Cas9 (SpCas9) as an example and show an example of its use in mammalian cells, HEK293FT cells.作者: orthodox 時(shí)間: 2025-3-27 12:26 作者: Ovulation 時(shí)間: 2025-3-27 17:13 作者: 到婚嫁年齡 時(shí)間: 2025-3-27 20:01
Engineered Protease-Responsive RNA-Binding Proteins (RBPs) to Expand the Toolbox of Synthetic Circue sophisticated and multilayered regulatory networks, addressing the previous limitation of regulatory orthogonal parts that can be assembled in synthetic devices. In this chapter, we describe the engineering and tests of protease-responsive RNA-binding proteins (L7Ae and MS2-cNOT7) to expand the toolbox of synthetic circuits in mammalian cells.作者: 不愿 時(shí)間: 2025-3-27 22:33 作者: Repatriate 時(shí)間: 2025-3-28 02:58
Detection of MicroRNAs Using Synthetic Toehold Switch in Mammalian Cells,tic toehold switch for detection of exogenously and endogenously expressed miRNAs in CHO, HeLa, HEK 293, and MDA-MB-231 breast cancer cells. Multiplex detection of miR-155 and miR-21 was tested using two toehold switches to evaluate the orthogonality and programmability of this synthetic platform.作者: CAMEO 時(shí)間: 2025-3-28 08:59 作者: CROW 時(shí)間: 2025-3-28 12:16 作者: anarchist 時(shí)間: 2025-3-28 17:34 作者: 火光在搖曳 時(shí)間: 2025-3-28 22:14 作者: 宣誓書 時(shí)間: 2025-3-28 23:06
Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems, modules effecting protein expression with a focus on minimizing the cloning burden in coding regions. The original MoPET system consists of 53 defined DNA modules divided into eight functional main classes and can be flexibly expanded dependent on the need of the experimenter and expression host. H作者: 收養(yǎng) 時(shí)間: 2025-3-29 05:13 作者: endocardium 時(shí)間: 2025-3-29 08:30
Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems,controlling CRISPR effectors in heterologous systems, including mammalian cells..Here, we review the diverse applications of Acrs in mammalian cells and organisms and discuss the underlying engineering strategies. These applications include (i) persistent blockage of CRISPR-Cas function to create wr作者: Arrhythmia 時(shí)間: 2025-3-29 12:55 作者: Etching 時(shí)間: 2025-3-29 15:37 作者: GONG 時(shí)間: 2025-3-29 23:31 作者: biosphere 時(shí)間: 2025-3-30 00:10 作者: Discrete 時(shí)間: 2025-3-30 06:26 作者: Addictive 時(shí)間: 2025-3-30 09:40
1064-3745 voiding known pitfalls..?..Authoritative and cutting-edge,?.Mammalian Synthetic Systems .aims to ensure successful results in the further study of this vital field..978-1-0716-3720-3978-1-0716-3718-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: condone 時(shí)間: 2025-3-30 15:09 作者: 獨(dú)特性 時(shí)間: 2025-3-30 18:26 作者: Urea508 時(shí)間: 2025-3-31 00:08 作者: 侵略者 時(shí)間: 2025-3-31 02:09
Call Attention to Rumors: Deep Attention Based Recurrent Neural Networks for Early Rumor Detectionunking rumors at their stage of diffusion is known as ., which refers to dealing with sequential posts regarding disputed factual claims with certain variations and highly textual duplication over time. Thus, identifying trending rumors demands an efficient yet flexible model that is able to capture作者: Aboveboard 時(shí)間: 2025-3-31 07:35
Fertility and Sexuality: Theoretical and Empirical Contributions from the Biological and Behavior Sciences Edited by Joseph Lee Rodgers, David C. Rowe, & Warren B. Miller Published by Kluwer Academic Press, 2000 The Biodemography of Human Reproduction and Fertility Edited by Joseph Lee Rodgers & Han作者: CREEK 時(shí)間: 2025-3-31 12:08 作者: 小木槌 時(shí)間: 2025-3-31 15:33 作者: BULLY 時(shí)間: 2025-3-31 21:00 作者: malapropism 時(shí)間: 2025-4-1 01:32
Jay M. Ver Hoef,David C. Glenn-Lewin,Marinus J. A. Wergerions of consensus democracy, intraparty consociationalism was found to co-exist with and draw strength from majoritarian institutions. The previous chapter therefore confirmed the hypothesis that, as dominant parties, consociational parties benefit from majoritarian institutions, making representati作者: JOT 時(shí)間: 2025-4-1 05:01
9 Tijd en identiteiten van zijn leerlingen. Deze heeft vermoedelijk ook de hiernavolgende passage braaf genoteerd: ‘Iemand, die met enig succes zijn talenten gebruikt heeft en zodoende naam gemaakt heeft in de wereld, zou al tevreden moeten zijn dat hem vermaardheid beschoren is; maar het is nu eenmaal zo dat men zich 作者: pellagra 時(shí)間: 2025-4-1 09:45 作者: MAIZE 時(shí)間: 2025-4-1 13:44
Springer Tracts in Modern Physics 36978-3-540-37095-6Series ISSN 0081-3869 Series E-ISSN 1615-0430 作者: PLIC 時(shí)間: 2025-4-1 15:19 作者: ethereal 時(shí)間: 2025-4-1 21:18
code is often seen as a blocker when it comes to implementing any kind of testing..? .What You Will Learn.Fit test to legacy code retrospectively.Install and configure popular Swift BDD frameworks.Practice BDD with Xcode.Who This Book Is For.Software practitioners, such as Swift developers and978-1-4842-2688-9978-1-4842-2689-6
