作者: STENT 時(shí)間: 2025-3-21 20:23 作者: 延期 時(shí)間: 2025-3-22 01:52
1064-3745 n advice from the experts.This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful .Methods in Molecular Biology .series 作者: Fatten 時(shí)間: 2025-3-22 07:20 作者: 收養(yǎng) 時(shí)間: 2025-3-22 12:35
Monitoring Promoter Activity by Flow Cytometryow cytometry and represents a readout for the promoter activities. In this chapter, the protocol for measurement and analyzing of transfected cells expressing the reporter gene EGFP is thoroughly described and fully illustrated.作者: Terrace 時(shí)間: 2025-3-22 15:53 作者: motivate 時(shí)間: 2025-3-22 20:57
Initial Considerations Before Designing a Promoter Construct,or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In this chapter I highlight some of the vector attributes to consider and features that should be thought about.作者: 放大 時(shí)間: 2025-3-23 00:32
Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assayalso to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.作者: FAZE 時(shí)間: 2025-3-23 03:00
Secreted Reporters for Monitoring Multiple Promoter Function applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three commonly used reporters: secreted alkaline phosphatase (SEAP), . luciferase (Gluc), and . luciferase (Vluc).作者: 為現(xiàn)場(chǎng) 時(shí)間: 2025-3-23 07:48 作者: VAN 時(shí)間: 2025-3-23 12:13
The Tetracycline Responsive System therapy. We have utilized pharmacologically regulated gene expression systems to achieve fine control of gene expression levels which facilitate research in basic biology and translates to use in experimental gene therapy studies. In this chapter, we outline the application of a tightly controlled tetracycline responsive gene expression system.作者: 入會(huì) 時(shí)間: 2025-3-23 17:48
Light-Responsive Promotersl control of gene expression in a so far unprecedented manner..In this chapter, we first describe the available light-inducible gene expression systems compatible with mammalian cells and explain their underlying mechanisms. Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells.作者: subordinate 時(shí)間: 2025-3-23 19:55
Promoter Activation with Electromagnetismield response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a step-by-step guide to the assembly of a solenoid suitable for promoter activation.作者: 積習(xí)已深 時(shí)間: 2025-3-23 22:42 作者: NICHE 時(shí)間: 2025-3-24 02:32 作者: 喚起 時(shí)間: 2025-3-24 09:18 作者: 潔凈 時(shí)間: 2025-3-24 14:36
Book 2017nthetic promoters with examples of their application. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and 作者: Acclaim 時(shí)間: 2025-3-24 17:34
978-1-4939-8421-3Springer Science+Business Media LLC 2017作者: 范例 時(shí)間: 2025-3-24 22:09
Mammalian Synthetic Promoters978-1-4939-7223-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Intact 時(shí)間: 2025-3-25 02:22
David GouldIncludes cutting-edge methods protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: escalate 時(shí)間: 2025-3-25 06:10 作者: Condescending 時(shí)間: 2025-3-25 09:08
https://doi.org/10.1007/978-1-4939-7223-4synthetic promoters; gene therapy; pharmacologically regulated expression; promoter sequences; vector co作者: Transfusion 時(shí)間: 2025-3-25 13:38
Ghazal Lashgari,Rami S. Kantar,Bakhos A. Tannous Ph.D.作者: 治愈 時(shí)間: 2025-3-25 16:01
Juliette M. K. M. Delhove,Rajvinder Karda,Kate E. Hawkins,Lorna M. FitzPatrick,Simon N. Waddington,T作者: Neutral-Spine 時(shí)間: 2025-3-25 22:32
Dan Y. Even,Adi Kedmi,Diana Ideses,Tamar Juven-Gershon作者: 昏暗 時(shí)間: 2025-3-26 00:21 作者: 不透明性 時(shí)間: 2025-3-26 08:14 作者: 滑動(dòng) 時(shí)間: 2025-3-26 10:55 作者: 青春期 時(shí)間: 2025-3-26 16:05
Eduardo G. Cafferata,Maria Veronica Lopez,Felipe J. Nu?ez,Maria A. R. Maenza,Osvaldo L. Podhajcer作者: Coeval 時(shí)間: 2025-3-26 19:05 作者: 蛙鳴聲 時(shí)間: 2025-3-26 21:30 作者: entitle 時(shí)間: 2025-3-27 02:57
Bioinformatically Informed Design of Synthetic Mammalian Promotersccur in identified gene modules, and the length of identified .-regulatory regions. Our method allows for the generation of synthetic promoter libraries with increased level of specificity and facilitates the selection of promoters that are highly active only under predefined gene expression profile作者: Filibuster 時(shí)間: 2025-3-27 06:44 作者: 慢慢沖刷 時(shí)間: 2025-3-27 10:50
Initial Considerations Before Designing a Promoter Construct,short-term promoter activity from an episomal vector, or does the promoter eventually need to be permanently active and be integrated into the genome or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In作者: morale 時(shí)間: 2025-3-27 14:16
Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assayter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demon作者: 狂怒 時(shí)間: 2025-3-27 20:58 作者: HUSH 時(shí)間: 2025-3-27 23:45
Secreted Reporters for Monitoring Multiple Promoter Functioned medium of cultured cells or body fluids such as blood and urine, ex vivo. In this chapter, we will explore established secreted reporters and their applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three co作者: antiquated 時(shí)間: 2025-3-28 03:10
Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivolar biology. Luciferase catalyzes an enzymatic reaction, which in the presence of the substrate luciferin produces photons of light relative to its molar concentration. The luciferase transgene can be genetically inserted at the first intron of a target gene to act as a surrogate for the gene’s endo作者: tenuous 時(shí)間: 2025-3-28 06:59
Monitoring Promoter Activity by Flow Cytometryg reporter gene systems is fundamental for pharmaceutical, biomedical, and molecular biology research. Genetic reporters are used not only for measuring promoter activities but also for understanding the mechanisms controlling gene transcription and in the?identification, and characterization of .-a作者: Blatant 時(shí)間: 2025-3-28 14:28 作者: 束縛 時(shí)間: 2025-3-28 17:24
Bioinformatically Informed Design of Synthetic Mammalian Promotersn. Typically, .-regulatory regions from a few genes are randomly combined to generate a synthetic promoter library, and the sequences with the highest activity are selected for in target cell lines. Here we describe a novel approach that can be employed in the construction of synthetic promoters. Sp作者: Small-Intestine 時(shí)間: 2025-3-28 20:33 作者: 假 時(shí)間: 2025-3-29 02:11
Constructing Strong Cell Type-Specific Promoters Through Informed Designtype-specific promoter regulation is a function of each cell’s unique complement of transcriptional machinery components. Accordingly, to achieve high levels of transcriptional activity within a particular cell type, synthetic promoters must be specifically designed to harness those cells discrete r作者: Temporal-Lobe 時(shí)間: 2025-3-29 06:26
PCR Assembly of Synthetic Promotersealing between complimentary DNA sequences to build random TFBS combinations within the assembled PCR products. A DNA polymerase is then employed to fill in the unpaired nucleotides in the generated sequences and also to amplify the assembled PCR products. We have used this method to generate synthe作者: Urologist 時(shí)間: 2025-3-29 11:17
The Tetracycline Responsive Systemtal in studies examining biological roles of proteins, or continued expression may be unnecessary after therapeutic effects have been achieved in gene therapy. We have utilized pharmacologically regulated gene expression systems to achieve fine control of gene expression levels which facilitate rese作者: 消息靈通 時(shí)間: 2025-3-29 12:17
Light-Responsive Promotersgulated systems. The latest generation of those light-regulated systems that are specifically responsive to different wavelengths allows spatiotemporal control of gene expression in a so far unprecedented manner..In this chapter, we first describe the available light-inducible gene expression system作者: 極大的痛苦 時(shí)間: 2025-3-29 18:45 作者: 膠水 時(shí)間: 2025-3-29 23:00
Promoter Activation with Electromagnetismiation. Promoter activation with extremely low-frequency electromagnetic fields is possible with an appropriate promoter, containing electromagnetic field response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a ste作者: gout109 時(shí)間: 2025-3-30 01:54 作者: angina-pectoris 時(shí)間: 2025-3-30 05:47
Ryohei Ogawa Ph.D.,Go Kagiya,Akihiko Watanabe,Akihiro Morii,Zheng-Guo Cui,Takashi Kondoss the types of changes in the magnetic and superconducting properties which can occur when a solid is subjected to high pressures. The changes observed for the elemental solids across the periodic table are representative for all compounds and alloys. The only elemental solids which exhibit strong 作者: Lumbar-Stenosis 時(shí)間: 2025-3-30 08:28 作者: 潰爛 時(shí)間: 2025-3-30 15:05 作者: 解開 時(shí)間: 2025-3-30 18:38
T. Ziegenfu?a coherent national economic plan although a step in this direction had taken place in the Second Plan (1954–7), which represented a considerable extension of the plan from the few key sectors of the First (Monnet) Plan.作者: eulogize 時(shí)間: 2025-3-30 23:43
Biology of Cellular Transducing Signalsd. Wir wollen hier kurz zun?chst die Eigenschaften der Dipolstrahlung darstellen und erst dann zur Quadrupolstrahlung übergehen, um so die ?hnlichkeiten und charakteristischen Unterschiede zwischen beiden festzustellen.作者: 起皺紋 時(shí)間: 2025-3-31 01:09 作者: GRAZE 時(shí)間: 2025-3-31 07:44
