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標(biāo)題: Titlebook: Lysosomes; Methods and Protocol Karin ?llinger,Hanna Appelqvist Book 2017 Springer Science+Business Media LLC 2017 apoptosis.cell repair.de [打印本頁(yè)]

作者: rupture    時(shí)間: 2025-3-21 19:59
書(shū)目名稱Lysosomes影響因子(影響力)




書(shū)目名稱Lysosomes影響因子(影響力)學(xué)科排名




書(shū)目名稱Lysosomes網(wǎng)絡(luò)公開(kāi)度




書(shū)目名稱Lysosomes網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書(shū)目名稱Lysosomes被引頻次




書(shū)目名稱Lysosomes被引頻次學(xué)科排名




書(shū)目名稱Lysosomes年度引用




書(shū)目名稱Lysosomes年度引用學(xué)科排名




書(shū)目名稱Lysosomes讀者反饋




書(shū)目名稱Lysosomes讀者反饋學(xué)科排名





作者: 消極詞匯    時(shí)間: 2025-3-21 23:22
Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes,d cells using highly specific monoclonal antibodies and fluorescence microscopy. The main advantage of this method is its sensitivity, and the fact that it provides spatial information on lysosomal exocytosis at the single cell level.
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作者: EXPEL    時(shí)間: 2025-3-22 05:45

作者: 鋼盔    時(shí)間: 2025-3-22 09:18
Analysis of ,- and ,-Glycosylation of Lysosomal Glycoproteins, specific glycosidases followed by a western blot analysis of a protein of interest provides a way to determine which types of glycans are present in the protein by comparing the gel mobility before and after treatment.
作者: Derogate    時(shí)間: 2025-3-22 13:06

作者: 會(huì)犯錯(cuò)誤    時(shí)間: 2025-3-22 18:29
Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells,c point, thereby the ratio can be used to determine the lysosomal pH. A standard curve is obtained by equilibrating intralysosomal pH with extracellular pH using the ionophore nigericin. The protocol also includes information regarding procedures to induce lysosomal alkalinization and lysosomal membrane permeabilization.
作者: PALL    時(shí)間: 2025-3-22 23:17
Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics,al lipidome by quantitatively detecting hundreds of lipid species of diverse physicochemical properties. We describe here a detailed protocol that couples isolation of superparamagnetic iron dextran-loaded lysosomes from cultured mammalian cell lines with quantitative mass spectrometry-based shotgun lipidomics.
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作者: Apoptosis    時(shí)間: 2025-3-23 11:17
Book 2017tocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Lysosome: Methods and Protocols.?aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle..
作者: 脆弱帶來(lái)    時(shí)間: 2025-3-23 16:00

作者: 一罵死割除    時(shí)間: 2025-3-23 21:07
1064-3745 ation advice from the experts.This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful .Methods in Molecular
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作者: 同音    時(shí)間: 2025-3-24 08:10
978-1-4939-8343-8Springer Science+Business Media LLC 2017
作者: 細(xì)胞    時(shí)間: 2025-3-24 11:04
Lysosomes978-1-4939-6934-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
作者: ferment    時(shí)間: 2025-3-24 18:30
https://doi.org/10.1007/978-1-4939-6934-0apoptosis; cell repair; degradation; homeostasis; plasma membrane
作者: Indolent    時(shí)間: 2025-3-24 20:36
Karin ?llinger,Hanna AppelqvistIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
作者: 騷擾    時(shí)間: 2025-3-25 01:39
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/589251.jpg
作者: 攤位    時(shí)間: 2025-3-25 04:30
Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry,it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.
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Ana Maria Vilamill Giraldo,Karin ?llinger,Vesa Loittoe Microsoft Teams in an effective way to achieve your global team goals..Leading a team is a challenge, but leading a team in the zoom economy can make you stressed out and overworked. Peter Ward gives the reader a communication and organization centered approach for the dynamic, hardworking, succes
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作者: textile    時(shí)間: 2025-3-27 00:17
Takanobu Otomo,Tamotsu Yoshimoriperating System (EOS).Helps you leverage hidden tools of PowManage and lead a team remotely by intertwining leadership principles with the many robust tools of Microsoft Teams.?This book shows you how to utilize Microsoft Teams in an effective way to achieve your global team goals..Leading a team is
作者: Commemorate    時(shí)間: 2025-3-27 04:54
Kristina M. Hamill,Ezequiel Wexselblatt,Wenyong Tong,Jeffrey D. Esko,Yitzhak Tore Microsoft Teams in an effective way to achieve your global team goals..Leading a team is a challenge, but leading a team in the zoom economy can make you stressed out and overworked. Peter Ward gives the reader a communication and organization centered approach for the dynamic, hardworking, succes
作者: CLASP    時(shí)間: 2025-3-27 05:29
Camilla L??v,Anna Erlandssonperating System (EOS).Helps you leverage hidden tools of PowManage and lead a team remotely by intertwining leadership principles with the many robust tools of Microsoft Teams.?This book shows you how to utilize Microsoft Teams in an effective way to achieve your global team goals..Leading a team is
作者: 放氣    時(shí)間: 2025-3-27 09:33
Ida Eriksson,Karin ?llinger,Hanna Appelqvistperating System (EOS).Helps you leverage hidden tools of PowManage and lead a team remotely by intertwining leadership principles with the many robust tools of Microsoft Teams.?This book shows you how to utilize Microsoft Teams in an effective way to achieve your global team goals..Leading a team is
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作者: 偶像    時(shí)間: 2025-3-27 18:18
Norma W. Andrewse Microsoft Teams in an effective way to achieve your global team goals..Leading a team is a challenge, but leading a team in the zoom economy can make you stressed out and overworked. Peter Ward gives the reader a communication and organization centered approach for the dynamic, hardworking, succes
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Yoko Hashimotomatik, die zukünftig verst?rkt in unseren Management-Studieng?ngen – ob für soziale Organisationen oder für Unternehmen – an Hochschulen in Deutschland wichtig wird und von daher auszubauen ist.. Wir wissen, dass insbesondere die sozialen Organisationen die staatliche Unterversorgung von Menschen mi
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Eser Y?ld?r?m Sozmen,Ebru Demirel Sezer vollem Ornat: dunkelgrüner Samttalar mit schwarzseidenem Innenfutter und einem Kragen aus orangeseidenem Velvet, Samtbarrett, Amtskette sowie das Kreuz des Ordens Pour le mérite (Buchardt 1996: 148). Es ist das Bild eines Mannes, eines hoch dekorierten Würdentr?gers aus einer vergangenen Zeit. Was
作者: 表兩個(gè)    時(shí)間: 2025-3-28 14:37
Jyotsna Verma,Sunita Bijarnia-Mahay,Ishwar C. VermaEurope’ (see, inter alia, George 1998; Gowland and Turner 2000; Wallace 1997). It has even been suggested that Britain is an ‘a(chǎn)llergic European’ (Aspinwall 2004). Such views have been shaped by Britain’s unwillingness to participate in the initial steps toward European integration in the 1950s and i
作者: 遵循的規(guī)范    時(shí)間: 2025-3-28 19:55
Colin Fennelly,Ravi K. Amaravadi been subjected to considerable academic scrutiny, the Council Secretariat1 has been all but discounted in the literature (Christiansen 2002; Christiansen and J?rgensen 1998; Dinan 2000; Gray 2002; Maurer 2002a; Moravcsik 1999a; Petite 2000; Corbett 1998). The Council Secretariat is on paper relativ
作者: Insubordinate    時(shí)間: 2025-3-29 01:35
SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS,ncreasing sensitivity and resolution of mass spectrometry in combination with labeling procedures which allow comparative quantitative proteomics can be applied to shed more light on the steadily increasing range of lysosomal functions. In addition, investigation of alterations in lysosomal protein
作者: Hyaluronic-Acid    時(shí)間: 2025-3-29 03:29

作者: 種植,培養(yǎng)    時(shí)間: 2025-3-29 11:11
Analysis of ,- and ,-Glycosylation of Lysosomal Glycoproteins,sosomal proteins of interest. The method is based on using deglycosylating enzymes, endoglycosidases, and exoglycosidases. Endoglycosidases catalyze the cleavage of an internal bond in an oligosaccharide, while exoglycosidases remove terminal carbohydrates from glycans. Different types of carbohydra
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作者: 遣返回國(guó)    時(shí)間: 2025-3-29 17:59

作者: 前奏曲    時(shí)間: 2025-3-29 21:38
Quantitative Co-Localization and Pattern Analysis of Endo-Lysosomal Cargo in Subcellular Image Cytons and lipids. Failure in such processes can lead to lysosomal storage disorders in which a particular metabolite accumulates within LE/LYSs. Analysis of endocytic trafficking relies heavily on quantitative fluorescence microscopy, but evaluation of the huge image data sets is challenging and demand
作者: nitroglycerin    時(shí)間: 2025-3-30 00:07
Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tisrations of H.O. in the lysosomes may cause redox imbalance and the loss of the critical functions of the lysosomes. Herein, we describe the preparation of a potent lysosome-targeted two-photon fluorescent probe (Lyso-HP) for the detection of H.O. in the lysosomes in the living cells. This unique flu
作者: Mangle    時(shí)間: 2025-3-30 05:25
Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery,xenophagy) to engulf by isolation membrane, and degrades toxic materials within lysosomes. We recently revealed that a membrane-damaged lysosome itself also becomes a target of autophagy and named this process lysophagy. In this chapter, we describe methods for monitoring lysophagy; detecting lysoso
作者: 繼而發(fā)生    時(shí)間: 2025-3-30 09:55
Delivery of Cargo to Lysosomes Using GNeosomes,alf-lives and minimized degradation. Here, we present a method for improving the selective delivery of liposomes to the lysosomes using a guanidinylated neomycin (GNeo) transporter. The method for synthesizing GNeo-lipids, incorporating them into liposomes, and the enhanced lysosomal delivery of enc
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作者: 連詞    時(shí)間: 2025-3-31 02:05
Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes,exocytosis in mammalian cells, which takes advantage of the presence of an abundant glycoprotein, Lamp1, on the membrane of lysosomes. Lamp1 is a transmembrane protein with a large, heavily glycosylated region that faces the lumen of lysosomes. When lysosomes fuse with the plasma membrane, epitopes
作者: 毀壞    時(shí)間: 2025-3-31 05:12

作者: 反復(fù)拉緊    時(shí)間: 2025-3-31 11:51
Next-Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Humarallel. One key application is the understanding of genetically heterogeneous and complex diseases where 50–100 different genes may converge to control the same pathways. These disorders cannot be studied using traditional approaches, based on gene-by-gene Sanger sequencing. We have set up an NGS pr
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作者: Aspiration    時(shí)間: 2025-3-31 18:29
,Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried on of undigested substrates from different catabolic pathways leads to cellular dysfunction. LSDs generally presents during early childhood and have a devastating impact on the families and on public health. Over the years, approaches for treatment of some LSDs have been developed with different str
作者: entail    時(shí)間: 2025-3-31 21:55
Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi,ochemical/molecular study in the index case is available and a strict defined protocol, specific to each individual disorder is followed. The present chapter describes the protocols for reliable and accurate prenatal enzymatic diagnoses by fluorometric and spectrophotometric methods of lysosomal sto
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Mesut Bilgin,Jesper Nylandsted,Marja J??ttel?,Kenji Maedayou will see how understanding your identity paves the way to achieving emancipation, capability, and autonomy. Ultimately, you will be empowered to lead with clarity. Your team has everything they need to exce978-1-4842-6299-3978-1-4842-6300-6
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Elmira Tokhtaeva,Olga A. Mareninova,Anna S. Gukovskaya,Olga Vaginyou will see how understanding your identity paves the way to achieving emancipation, capability, and autonomy. Ultimately, you will be empowered to lead with clarity. Your team has everything they need to exce978-1-4842-6299-3978-1-4842-6300-6
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