作者: 防止 時(shí)間: 2025-3-21 21:10
Da Wei Huang,Moez Dawood,Calvin A. Johnson,Roland Schmitzd it is hard to think of a historical parallel within which political elites and populations have worked towards a broad common goal so determinedly, albeit usually without direct cooperation. But agreement about specific goals has not been unified, because of an absence of a consensus about the sta作者: 柳樹(shù)；枯黃 時(shí)間: 2025-3-22 01:03 作者: Neuropeptides 時(shí)間: 2025-3-22 06:29
Daniel Hübschmann,Matthias Schlesneres findings from positive psychology and organizational beha.The COVID-19 pandemic has permanently changed lives around the world and no dimension of life and leadership seems to have been spared from its wrath. It has also stirred us into thinking about novel approaches to lead organizations and so作者: BADGE 時(shí)間: 2025-3-22 11:07
Daniel E. Webster,Sandrine Roulland,James D. Phelanhemen deutlich an.. Ein Teil der intensiven Aufbereitung der vielf?ltigen Führungsthemen hat zur wissenschaftlichen Aufl?sung von Führungsmythen (z.B. Great Man-Mythos oder Glaube an die ideale Führungsorganisation) beigetragen und eine gro?e Zahl popul?rer bis wissenschaftlich fundierter Ratschl?ge作者: 母豬 時(shí)間: 2025-3-22 12:53 作者: 擔(dān)心 時(shí)間: 2025-3-22 19:58
Ryan M. Young für den Leadership-Branding-Prozess.Pionierwissen zu einem .Die Kraft einer Marke kommt immer von innen. Deshalb kann eine Marke nicht stark werden ohne Führungskr?fte und Mitarbeiter, die die Marke leben. In markenspezifischer Führung stecken betr?chtliche, aber meist ungenutzte Produktivit?tsrese作者: insolence 時(shí)間: 2025-3-22 23:25
Bj?rn H?upl,Henning Urlaub,Thomas Oellerich für den Leadership-Branding-Prozess.Pionierwissen zu einem .Die Kraft einer Marke kommt immer von innen. Deshalb kann eine Marke nicht stark werden ohne Führungskr?fte und Mitarbeiter, die die Marke leben. In markenspezifischer Führung stecken betr?chtliche, aber meist ungenutzte Produktivit?tsrese作者: Fester 時(shí)間: 2025-3-23 05:21 作者: 確定的事 時(shí)間: 2025-3-23 05:45 作者: TRUST 時(shí)間: 2025-3-24 01:25
Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ-)PCR,lin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in 作者: Heart-Attack 時(shí)間: 2025-3-24 05:16
Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas, has led to the identification of significant repertoire restrictions, culminating in the discovery of subsets of patients expressing highly similar, stereotyped BcR IG. This finding strongly supports selection by common epitopes or classes of structurally similar epitopes in the ontogeny of these t作者: 細(xì)節(jié) 時(shí)間: 2025-3-24 09:40 作者: 事物的方面 時(shí)間: 2025-3-24 12:45 作者: 咽下 時(shí)間: 2025-3-24 16:42
Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input, DNA methylation changes are most effective in promoters and enhancers, the former frequently being CpG-rich and the latter, in contrast, CpG-poor. Many genome-wide methods for DNA methylation analysis interrogate predominantly CpG-rich regions and, hence, spare enhancers and other potentially impor作者: Conducive 時(shí)間: 2025-3-24 21:54
FISH and FICTION in Lymphoma Research,uclei. It is widely used in routine diagnostics to identify cancer-specific aberrations including lymphoma-associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping—a technique called fluorescence immunophenotyping and interphase cytogeneti作者: BLAZE 時(shí)間: 2025-3-25 00:19
NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma,iRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in作者: 滴注 時(shí)間: 2025-3-25 05:54
RNA Sequencing in B-Cell Lymphomas,NA (cDNA) to generate several millions of short sequence reads that are aligned to a reference genome allowing the comprehensive characterization of the transcripts in a cell. RNA-Seq has a wide variety of applications which lead to a pervasive adoption of this method well beyond the genomics commun作者: 悠然 時(shí)間: 2025-3-25 08:20
Studying Cancer Heterogeneity by Single-Cell RNA Sequencing,e. Genetic and epigenetic changes in combination with external cues and selective forces are the driving factors behind tumor heterogeneity. Understanding this variability within and across patients may partly explain the unpredictable outcomes of cancer treatments. Measuring the variation of gene e作者: 瑪瑙 時(shí)間: 2025-3-25 11:59
Evaluation of Whole Genome Sequencing Data, analysis of WGS data, including alignment, identification of different classes of genomic variants, the identification of driver mutations, and the identification of mutational signatures. We further outline design considerations for WGS studies and provide a variety of quality control measures to 作者: 哺乳動(dòng)物 時(shí)間: 2025-3-25 16:15
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines,the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomi作者: 我怕被刺穿 時(shí)間: 2025-3-25 22:01
Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens,ion of biomarkers associated with response to drugs would greatly improve the efficacy of drug treatment. Platforms that can comprehensively map drug response in high-throughput ex vivo provide a unique tool to identify associated biomarkers and provide hypotheses for mechanisms underlying variable 作者: hedonic 時(shí)間: 2025-3-26 02:58
Proximity Ligation Assay, technique used to examine protein-protein interactions in situ. PLA offers many advantages over traditional biochemical approaches to signaling in that it can detect both transient interactions, which are hard to capture otherwise, as well as protein interactions within large supramolecular signali作者: 樂(lè)意 時(shí)間: 2025-3-26 06:57 作者: 正論 時(shí)間: 2025-3-26 11:09
Ralf KüppersIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 關(guān)心 時(shí)間: 2025-3-26 15:14
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/589223.jpg作者: 變化無(wú)常 時(shí)間: 2025-3-26 20:21
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: GOUGE 時(shí)間: 2025-3-26 21:13 作者: indubitable 時(shí)間: 2025-3-27 03:04 作者: harangue 時(shí)間: 2025-3-27 06:58
Evaluation of Whole Genome Sequencing Data, analysis of WGS data, including alignment, identification of different classes of genomic variants, the identification of driver mutations, and the identification of mutational signatures. We further outline design considerations for WGS studies and provide a variety of quality control measures to detect common quality problems in the data.作者: Epidural-Space 時(shí)間: 2025-3-27 11:53 作者: 奇思怪想 時(shí)間: 2025-3-27 17:27
Ryan M. Young-Sport, Alfred Ritter GmbH & Co. KG.?.?Die Verantwortung der Marke liegt heutzutage bei jedem einzelnen Mitarbeiter. Christina Grubendorfer greift mit Ihrem Buch ein Thema unserer Zeit auf: Ein markenspezifisch978-3-8349-3706-3作者: Fatten 時(shí)間: 2025-3-27 20:57
Bj?rn H?upl,Henning Urlaub,Thomas Oellerich-Sport, Alfred Ritter GmbH & Co. KG.?.?Die Verantwortung der Marke liegt heutzutage bei jedem einzelnen Mitarbeiter. Christina Grubendorfer greift mit Ihrem Buch ein Thema unserer Zeit auf: Ein markenspezifisch978-3-8349-3706-3作者: BOOST 時(shí)間: 2025-3-28 01:28 作者: 甜得發(fā)膩 時(shí)間: 2025-3-28 05:27 作者: 惡意 時(shí)間: 2025-3-28 08:16
Andreas Agathangelidis,Fotis Psomopoulos,Kostas Stamatopoulos作者: Crater 時(shí)間: 2025-3-28 14:00
Christiane Pott,Monika Brüggemann,Matthias Ritgen,Vincent H. J. van der Velden,Jacques J. M. van Don作者: majestic 時(shí)間: 2025-3-28 15:43
Dieter Weichenhan,Charles D. Imbusch,Qi Wang,Benedikt Brors,Christoph Plass作者: SAGE 時(shí)間: 2025-3-28 20:53 作者: Affectation 時(shí)間: 2025-3-29 02:55 作者: 微塵 時(shí)間: 2025-3-29 06:33
Daniel E. Webster,Sandrine Roulland,James D. Phelaneugende Orientierungshilfen. Dies ist gerade bei grundlegenden Fragen kritisch. So bleibt zum Beispiel bis dato unklar, welche Denk- und Handlungsanforderungen an Führungskr?fte am Anfang des 21. Jahrhunderts gestellt werden müssen, um Unternehmen strategisch und operativ durch die turbulenten M?rkt作者: Conclave 時(shí)間: 2025-3-29 08:29 作者: Hemoptysis 時(shí)間: 2025-3-29 12:26 作者: 沒(méi)血色 時(shí)間: 2025-3-29 19:36
Expression Cloning of Antibodies from Single Human B Cells,the reverse transcription-polymerase chain reaction (RT-PCR)-based amplification of the expressed immunoglobulin (Ig) transcripts (., ., and . and their subsequent cloning into expression vectors for the in vitro production of recombinant monoclonal antibodies. The strategy may be used to obtain inf作者: 彩色的蠟筆 時(shí)間: 2025-3-29 23:32
Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas,d IG are mostly “disease-specific,” implicating distinct immunopathogenetic processes. Interestingly, mounting evidence suggests that a molecular subclassification of lymphomas based on BcR IG stereotypy is biologically and clinically relevant. Indeed, particularly in CLL, patients assigned to the s作者: FLOAT 時(shí)間: 2025-3-30 01:30 作者: Interregnum 時(shí)間: 2025-3-30 04:08
Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input,mentation generates DNA fragments flanked by uniform sequencing adapters. In a subsequent step, the non-covalently bound adapter oligonucleotide needs to be replaced by a novel oligonucleotide to provide the proper adapter sequence for the reverse strand in paired-end sequencing. The presented proto作者: Valves 時(shí)間: 2025-3-30 08:18
Studying Cancer Heterogeneity by Single-Cell RNA Sequencing, which shows great capability to generate transcriptome data for cancer cells. mcSCRB-seq is not only characterized by high sensitivity due to molecular crowding and the use of unique molecular identifiers (UMIs) but also features an easy workflow and a low per-cell cost and does not require special作者: defendant 時(shí)間: 2025-3-30 12:50 作者: 開(kāi)始從未 時(shí)間: 2025-3-30 16:33 作者: 加花粗鄙人 時(shí)間: 2025-3-31 00:00 作者: 伸展 時(shí)間: 2025-3-31 01:14
Joost Kluiver,Fubiao Niu,Ye Yuan,Klaas Kok,Anke van den Berg,Agnieszka Dzikiewicz-Krawczykundlage, aktuelle Aufgaben und Probleme der organisationalen Praxis zu bew?ltigen. Durch die ver?nderten Arbeitsbedingungen der Arbeit?4.0 ergeben sich zahlreiche Herausforderungen, mit denen Führungskr?fte in Unternehmen konfrontiert sind. Die Grenzen zw978-3-662-65721-8978-3-662-65722-5Series ISSN 2662-7612 Series E-ISSN 2662-7620 作者: 大約冬季 時(shí)間: 2025-3-31 06:12 作者: 小歌劇 時(shí)間: 2025-3-31 10:39 作者: emulsify 時(shí)間: 2025-3-31 17:03
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines,c DNA, and prepare a custom library for next-generation sequencing. These protocols were tailored for loss-of-function CRISPR screens in human lymphoma cell lines but are highly amenable for other experimental purposes.作者: 僵硬 時(shí)間: 2025-3-31 20:02 作者: antidote 時(shí)間: 2025-4-1 01:43
Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas,mal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma and nodular lymphocyte predominant Hodgkin lymphoma.作者: Engulf 時(shí)間: 2025-4-1 05:36 作者: NIL 時(shí)間: 2025-4-1 07:54 作者: CRACK 時(shí)間: 2025-4-1 10:32 作者: Debility 時(shí)間: 2025-4-1 15:24
1064-3745 ation advice from the expertsThis second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene k作者: IDEAS 時(shí)間: 2025-4-1 22:08 作者: 采納 時(shí)間: 2025-4-1 22:41 作者: 純樸 時(shí)間: 2025-4-2 03:54
PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clotocol covers the pre-analytical phase of DNA isolation (from formalin-fixed paraffin-embedded and fresh tissues, body fluids, peripheral blood, and bone marrow), the analytical phase of PCR GeneScan and heteroduplex analysis, and the post-analytical interpretation of the obtained profiles, following established guidelines.作者: ectropion 時(shí)間: 2025-4-2 07:07
Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ-)PCR,tion through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the . light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.作者: monochromatic 時(shí)間: 2025-4-2 12:30
y them. Non-verbal communication is all the ways we add or change meanings of the words actually spoken. In this chapter, we discuss some of the ways we are socialized in the use of non-verbal communication. It should be used to enhance your spoken message to improve clarity and increase credibility
