標(biāo)題: Titlebook: Listeria monocytogenes; Methods and Protocol Kieran Jordan,Edward M. Fox,Martin Wagner Book 2014 Springer Science+Business Media New York 2 [打印本頁] 作者: 明顯 時(shí)間: 2025-3-21 16:04
書目名稱Listeria monocytogenes影響因子(影響力)
作者: 性冷淡 時(shí)間: 2025-3-21 23:58
Multilocus Sequence Typing (MLST) of ,r methods as it can reconstruct ancestral and evolutionary linkage between . isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.作者: 明智的人 時(shí)間: 2025-3-22 01:10
Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of ,ated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype . strains.作者: Ardent 時(shí)間: 2025-3-22 07:05 作者: fulmination 時(shí)間: 2025-3-22 09:52 作者: 口訣法 時(shí)間: 2025-3-22 13:14
Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Method. For mutagenesis in ., we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding segment of chromosomal DNA: the pKSV7 system and the pORI280/pVE6007 system.作者: LIKEN 時(shí)間: 2025-3-22 17:45
Sampling the Processing Environment for ,to sample, the best time for sampling and the frequency of sampling are also given. Such details help hygienists/microbiologists to be successful in their attempts to isolate strains of ., even if such bacteria are well attached to surfaces or located in niches that are difficult to reach.作者: ANA 時(shí)間: 2025-3-22 21:33 作者: 含鐵 時(shí)間: 2025-3-23 04:15 作者: ROOF 時(shí)間: 2025-3-23 08:17
Extraction and Analysis of Plasmid DNA from ,ient for analytical purposes but may be upscaled for further downstream analysis. Electrophoretic separation of the resultant lysate allows conclusions to be made on the presence, number, copy number, and size of the plasmids in the analyzed bacterial strains.作者: Sarcoma 時(shí)間: 2025-3-23 11:21 作者: 連鎖 時(shí)間: 2025-3-23 15:46
Book 2014thods and Protocols. addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of .Listeria. at a molecular level. The topics covered include sampling in order to isolate .Listeria., methods for their identification and characterization, methods 作者: upstart 時(shí)間: 2025-3-23 19:45
1064-3745 Contains key notes and implementation advice from the expert.Listeria monocytogenes is still a major threat to public health. A new book in the .Methods in Molecular Biology. series, .Listeria monocytogenes: Methods and Protocols. addresses its titular pathogen with protocols and methodologies used 作者: septicemia 時(shí)間: 2025-3-24 01:53
1064-3745 ative, .Listeria monocytogenes: Methods and Protocols. aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research..978-1-4939-5436-0978-1-4939-0703-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 窒息 時(shí)間: 2025-3-24 04:42 作者: 爆米花 時(shí)間: 2025-3-24 09:34
David Rodríguez-Lázaro,Marta Hernándezelbst grob ausgeschiedenen Zementits. Die L?sung greift aber auch den Wüstit merklich an, allerdings ohne seine Struktur zu ver?ndern. In technischen unberuhigten und teil beruhigten St?hlen erfahren bei l?ngerem Glühen zwischen 800 und 1200°C der Wüstit und die daneben auftretenden Silikate starke Ver?nderun978-3-663-06354-4978-3-663-07267-6作者: 水汽 時(shí)間: 2025-3-24 14:44 作者: inchoate 時(shí)間: 2025-3-24 17:58
Lisa Gorskihend dem Punkt m in diesem Punkt als reines . -Eisen kristallisieren, sofern man die Schmelze sehr langsam abkühlt und sich das Gleichgewicht stets ausbilden kann. Bei der au?erordentlich geringen Beweglichkeit des Phosphors im . -Eisen ist aber eine solche Gleichgewichtslage nur sehr schwer einzust作者: 大喘氣 時(shí)間: 2025-3-24 22:39
Marion Dalmasso,Kieran Jordanhend dem Punkt m in diesem Punkt als reines . -Eisen kristallisieren, sofern man die Schmelze sehr langsam abkühlt und sich das Gleichgewicht stets ausbilden kann. Bei der au?erordentlich geringen Beweglichkeit des Phosphors im . -Eisen ist aber eine solche Gleichgewichtslage nur sehr schwer einzust作者: 即席 時(shí)間: 2025-3-25 02:10
Beatrix Stessl,Irene Rückerl,Martin Wagnerisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: 長矛 時(shí)間: 2025-3-25 06:19
Mazin Matloob,Mansel Griffithsisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: Indict 時(shí)間: 2025-3-25 11:33
Corinne Amarisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: capsaicin 時(shí)間: 2025-3-25 13:04 作者: 傷心 時(shí)間: 2025-3-25 16:56
Michel Hébraudisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: nutrition 時(shí)間: 2025-3-25 22:55
Marcel R. Eugster,Martin J. Loessnerisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: Recess 時(shí)間: 2025-3-26 00:33 作者: 不知疲倦 時(shí)間: 2025-3-26 04:29
Swetha Reddy,Mark L. Lawrenceisomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: 詞匯表 時(shí)間: 2025-3-26 11:21 作者: curriculum 時(shí)間: 2025-3-26 16:15
isomonodromic deformations of linear differential equations is also developed...Aimed at graduate students and researchers, the book assumes some familiarity with basic complex algebraic geometry..978-1-84800-053-7978-1-84800-054-4Series ISSN 0172-5939 Series E-ISSN 2191-6675 作者: 排出 時(shí)間: 2025-3-26 18:29
Aidan Casey,Olivia McAuliffeJ. Bourgain‘s proof of the dimension conjecture, L. Carleson‘s biorthogonal system in H1, T. Figiel‘s integral representation, W.B. Johnson‘s factorization of operators, B. Maurey‘s isomorphism, and P. Jones‘ proof of the uniform approximation property. An entire chapter is devoted to the study of c作者: finite 時(shí)間: 2025-3-26 21:37
Kathrin Rychli,Caitriona M. Guinane,Karen Daly,Colin Hill,Paul D. CotterJ. Bourgain‘s proof of the dimension conjecture, L. Carleson‘s biorthogonal system in H1, T. Figiel‘s integral representation, W.B. Johnson‘s factorization of operators, B. Maurey‘s isomorphism, and P. Jones‘ proof of the uniform approximation property. An entire chapter is devoted to the study of c作者: 過份艷麗 時(shí)間: 2025-3-27 03:41
Book 2014tory protocols and tips on troubleshooting and avoiding known pitfalls..Vital and authoritative, .Listeria monocytogenes: Methods and Protocols. aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research..作者: Inveterate 時(shí)間: 2025-3-27 07:32
Ribotyping and Automated Ribotyping of ,n probe, electrophoretic gel, and membrane have been developed to deliver consistent performance. Data capture is accomplished via a CCD camera and the gel patterns obtained stored in a digitized format, making it easier to compare results among laboratories and to exchange data electronically.作者: Ischemic-Stroke 時(shí)間: 2025-3-27 12:41 作者: Badger 時(shí)間: 2025-3-27 16:44
Sampling the Processing Environment for ,contact surfaces, and liquids such as drain effluents are addressed. Sponge stick swabs are considered advantageous for surface sampling and tips regarding their application are given. Liquids are collected using sterile dippers and the procedure for their correct use is described. Advice on places 作者: Indicative 時(shí)間: 2025-3-27 18:59 作者: Mirage 時(shí)間: 2025-3-28 00:51 作者: Melatonin 時(shí)間: 2025-3-28 04:12
Pulsed-Field Gel Electrophoresis (PFGE) Analysis of ,ded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes. As DNA fragments are too large to be separated by traditional electrophoresis in an agarose gel, changes in the direction of the electrical current are periodically applied in orde作者: inchoate 時(shí)間: 2025-3-28 07:10
Multilocus Sequence Typing (MLST) of ,ory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available. MLST has advantages over other methods as it can reconstruct ancestral and evolutionary linkage between . isolates. MLST detects all genetic variations within the amplified housek作者: 同位素 時(shí)間: 2025-3-28 10:42 作者: 群居男女 時(shí)間: 2025-3-28 15:39 作者: Frenetic 時(shí)間: 2025-3-28 22:41
High-Throughput Characterization of , Using the OmniLog Phenotypic Microarrayphenotype responses, increase the output of such investigations and allow a more complete overview of the bacterial phenotype, and how this may relate its genotype. This chapter describes the application of OmniLog Phenotype Microarray analysis, a high-throughput assay for the phenotypic characteriz作者: Condescending 時(shí)間: 2025-3-29 01:46
Analysis of , Subproteomesar physiology. Like for other living organisms, using the tools of proteomics has undoubtedly improved knowledge about the foodborne pathogen .. Among the different technologies and approaches permanently evolving in the field of proteomics, the 2-DE is an analytical separation method of choice to r作者: DUCE 時(shí)間: 2025-3-29 03:22 作者: BINGE 時(shí)間: 2025-3-29 08:09 作者: Euthyroid 時(shí)間: 2025-3-29 13:24
Virulence Characterization of , and causes listeriosis more frequently in immunocompromised individuals. Virulence mechanisms in . are highly regulated and tightly controlled. A number of virulence factors that play important roles in pathogenesis of listeriosis have been identified and characterized. This review highlights the p作者: Aqueous-Humor 時(shí)間: 2025-3-29 17:08 作者: linear 時(shí)間: 2025-3-29 21:58
Extraction and Analysis of Plasmid DNA from ,esting and alkaline lysis of the bacteria and precipitation of chromosomal DNA and protein, followed by purification of the plasmid DNA. Here, we describe the mini-preparation of plasmid DNA by a rapid small-scale method, adapted for .. The quality of plasmid DNA isolated using this method is suffic作者: Irritate 時(shí)間: 2025-3-30 00:08
Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Methoddepth protocols for the creation of nonpolar unlabeled gene deletions in . (..) . that are facilitated by the splicing overlap extension PCR technique. For mutagenesis in ., we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the作者: 固定某物 時(shí)間: 2025-3-30 07:53
Rui Magalh?es,Cristina Mena,Vania Ferreira,Gon?alo Almeida,Joana Silva,Paula Teixeira Bei anodischer Aufl?sung wird er passiviert. Er kann in neutralen und schwachal- kalischen Elektrolyten praktisch vollst?ndig aus St?hlen freigelegt werden. Um Nebenreaktionen zu vermeiden, ist es zweckm??ig, die Elektrolysen unter poten- tiostatischen Bedingungen durchzuführen. Die Abtrennung des 作者: 得罪 時(shí)間: 2025-3-30 12:06
David Rodríguez-Lázaro,Marta Hernándezngreifbar. Bei anodischer Aufl?sung wird er passiviert. Er kann in neutralen und schwachal- kalischen Elektrolyten praktisch vollst?ndig aus St?hlen freigelegt werden. Um Nebenreaktionen zu vermeiden, ist es zweckm??ig, die Elektrolysen unter poten- tiostatischen Bedingungen durchzuführen. Die Abtre作者: installment 時(shí)間: 2025-3-30 12:22 作者: 的染料 時(shí)間: 2025-3-30 17:38 作者: HAUNT 時(shí)間: 2025-3-30 21:49
