作者: 飾帶 時(shí)間: 2025-3-21 22:10 作者: 過(guò)時(shí) 時(shí)間: 2025-3-22 01:28 作者: hurricane 時(shí)間: 2025-3-22 05:54
Axelle Grelard,Anthony Couvreux,Cécile Loudet,Erick J. Dufourc作者: 無(wú)政府主義者 時(shí)間: 2025-3-22 12:44 作者: Seminar 時(shí)間: 2025-3-22 13:43 作者: enumaerate 時(shí)間: 2025-3-22 20:17
Rosanna Cazzolli,Ping Huang,Shuzhi Teng,William E Hughes作者: 消音器 時(shí)間: 2025-3-22 21:57
Book 2009tips on troubleshooting and avoiding known pitfalls...Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology..作者: 打擊 時(shí)間: 2025-3-23 01:36
Inositol Lipid-Dependent Functions in ,: Analysis of Phosphatidylinositol Phosphatess because the cell wall must be broken in order to facilitate solvent access and maximize lipid yield. Once lipids have been extracted, fatty acids are removed and the resulting water-soluble glycerophosphoinositol phosphates are analysed by anion-exchange HPLC. This chapter describes how to extract作者: 征稅 時(shí)間: 2025-3-23 06:32 作者: Asymptomatic 時(shí)間: 2025-3-23 12:52
Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea lid-state nuclear magnetic resonance (NMR) spectroscopy. Colorimetric assays determine the amounts of inorganic phosphates from phospholipids and cholesterol/cholesteryl esters present in membrane-containing fractions. Filipin staining of natural membranes allows the localization and relative quanti作者: Temporal-Lobe 時(shí)間: 2025-3-23 16:44
Solution and Solid-State NMR of Lipidsr solid-state NMR provides information on the nature of the membrane phase (lamellar, hexagonal, isotropic, etc.), its dynamics (fluid or gel, or liquid ordered with cholesterol), and the molecular structure of embedded lipids when using the magic angle spinning apparatus. Typical examples of relati作者: 和藹 時(shí)間: 2025-3-23 19:25
Spatial Localization of PtdIns,, in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLan therefore show how membrane physical properties can affect enzyme binding to phosphatidylinositol 4,5-bisphosphate and thus further the understanding of important membrane processes such as endocytosis.作者: 擴(kuò)音器 時(shí)間: 2025-3-23 23:15
Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes production, via sphingomyelinase action or addition of external ceramide, induces the transbilayer lipid motion of the lipids within the cellular membrane. This chapter outlines various commonly used assays for measuring lipid flip-flop induced by ceramide in cell and model membranes.作者: Acupressure 時(shí)間: 2025-3-24 02:47 作者: 貧窮地活 時(shí)間: 2025-3-24 07:58 作者: figment 時(shí)間: 2025-3-24 11:00
Xavier Mulet,Erika Rosivatz,Ka Kei Ho,Béatrice L.L.E. Gauthé,Oscar Ces,Richard H. Templer,Rudiger Woniche, and successful firms may price above marginal cost. The resulting profit flows are channelled to the firms‘ shareholders. The introduction of monopolistic competition into growth theory is valuable: real world economies may be portrayed rather by such an imperfect competition framework than b作者: 尖叫 時(shí)間: 2025-3-24 17:38
F.-Xabier Contreras,Ana-Victoria Villar,Alicia Alonso,Félix M. Go?i successful firms may price above marginal cost. The resulting profit flows are channelled to the firms’ shareholders. The introduction of monopolistic competition into growth theory is valuable: real world economies may be portrayed rather by such an imperfect competition framework than by a perfec作者: 不可侵犯 時(shí)間: 2025-3-24 20:30
Eun-Young Lee,Pan-Young Jeong,Sun-Young Kim,Yhong-Hee Shim,David J. Chitwood,Young-Ki Paikniche, and successful firms may price above marginal cost. The resulting profit flows are channelled to the firms‘ shareholders. The introduction of monopolistic competition into growth theory is valuable: real world economies may be portrayed rather by such an imperfect competition framework than b作者: COLIC 時(shí)間: 2025-3-25 01:28 作者: 馬籠頭 時(shí)間: 2025-3-25 05:38
Marie Hélène Paclet,Jan K. Davidson-Moncada,Guillermo López-Lluch,Dongmin Shao,Lodewijk V. Dekker anderen Gebietsk?rperschaften sowie von Unternehmungen. In einer gro?en Investmentbank sind diese zwei Bereiche getrennt, weil sehr unterschiedliche F?higkeiten gefragt sind. Besonders bei der Finanzierung von Staaten sind die Produkte gegeben. Es ist von viel gr??erem Interesse, die ben?tigten Gel作者: calumniate 時(shí)間: 2025-3-25 09:19
Lipid Signaling Protocols978-1-60327-115-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Thyroiditis 時(shí)間: 2025-3-25 13:26 作者: Supplement 時(shí)間: 2025-3-25 18:19 作者: Brocas-Area 時(shí)間: 2025-3-25 21:34
https://doi.org/10.1007/978-1-60327-115-8Cell function; Lipid analysis; Lipid dependent signaling; Lipid metabolism; Metabolites; Metabolizing enz作者: rods366 時(shí)間: 2025-3-26 02:03
Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstproaches also represent alternative means for gauging the phosphoinositide-3?kinase/Akt activity. We describe protocols for the fractionation of cytoplasmic and nuclear protein extracts and for studying transcription factor DNA-binding activity . and .作者: 猛然一拉 時(shí)間: 2025-3-26 05:10 作者: 挫敗 時(shí)間: 2025-3-26 11:44 作者: 燈絲 時(shí)間: 2025-3-26 15:33 作者: eczema 時(shí)間: 2025-3-26 18:55 作者: 胰臟 時(shí)間: 2025-3-26 23:15 作者: 高度表 時(shí)間: 2025-3-27 03:49 作者: puzzle 時(shí)間: 2025-3-27 06:39
Eicosanoids: Generation and Detection in Mammalian Cells in general. This review focuses on the available methods for the detection and analysis of eicosanoids and aims to act as a guide for those wishing to approach the analysis of eicosanoids for their own research.作者: 粗魯?shù)娜?nbsp; 時(shí)間: 2025-3-27 10:38
Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranesy labeled streptavidin-monobiotinylated anti-HEL antibody bridge. This allows us to study the interaction of HEL-specific B cells with the tethered antigen by a variety of microscopy techniques. We recently used this system to study the activation of B cells by membrane antigens.作者: Fibrinogen 時(shí)間: 2025-3-27 15:32 作者: 大量 時(shí)間: 2025-3-27 18:44 作者: septicemia 時(shí)間: 2025-3-27 23:24 作者: BALE 時(shí)間: 2025-3-28 02:48 作者: 缺乏 時(shí)間: 2025-3-28 09:51 作者: 抑制 時(shí)間: 2025-3-28 11:28
Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometrular behavior. In humans, inappropriate changes may manifest as diseases such as Alzheimer’s, atherosclerosis, diabetes, obesity, and even cancer. This has led to a great interest in monitoring lipid changes both during normal cellular processes and in disease situations. With the advent of rapid ma作者: Affiliation 時(shí)間: 2025-3-28 17:29
Measurement of Polyphosphoinositides in Cultured Mammalian Cellsponent of eukaryotic cell membranes. Nevertheless, their synthesis is needed for an ever-increasing spectrum of cellular processes, including regulation of the actin cytoskeleton, chemotaxis, membrane trafficking, glucose uptake, and organelle acidification. PPIn metabolism is regulated dynamically 作者: 災(zāi)禍 時(shí)間: 2025-3-28 18:58 作者: Spangle 時(shí)間: 2025-3-29 00:14
Methods for the Determination of the Mass of Nuclear PtdIns4,, PtdIns5,, and PtdIns(4,5),,undance, they form a dynamic signaling system that is regulated in response to a variety of extra- and intracellular cues [.]. Protein domains including PH, FYVE, ENTH, PHOX, PHD fingers, and lysine-/arginine-rich patches can bind to specific phosphoinositide isomers, which, in turn, can induce chan作者: 可行 時(shí)間: 2025-3-29 06:16 作者: deceive 時(shí)間: 2025-3-29 08:56
Solution and Solid-State NMR of Lipidsathways, as they are recognition sites at the membrane surface. Their head-group or chain structure appears to be crucial for such a signaling role. Other lipids such as cholesterol and sphingomyelin are key molecules in maintaining membrane integrity and are the building blocks of membrane domains,作者: Tractable 時(shí)間: 2025-3-29 11:38 作者: 我的巨大 時(shí)間: 2025-3-29 16:00 作者: 阻擋 時(shí)間: 2025-3-29 20:19 作者: 背帶 時(shí)間: 2025-3-30 01:44
Effects of Sterols on the Development and Aging of ,es extensive enzymatic modification in . to form certain sterols of unknown function. Since sterol metabolism in . differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in ., a sterol-feedin作者: SOBER 時(shí)間: 2025-3-30 07:20
Proteomic Analysis of the Sterol-Mediated Signaling Pathway in , into cholesterol for cellular function. The inhibition of sterol conversion to cholesterol in . by various sterol biosynthesis inhibitors (SBIs) is known to cause serious defects in the development of these worms. To determine the biochemical consequences of these physiological abnormalities, one c作者: modest 時(shí)間: 2025-3-30 10:53 作者: conjunctivitis 時(shí)間: 2025-3-30 13:58
Measurement of PTEN Activity , by Imaging Phosphorylated Aktephosphorylating 3-phosphorylated phosphoinositides. Therefore, PtdIns(3,4,5)P.-dependent activation and phosphorylation of the survival kinase Akt can be used as readout for cellular PTEN activity. Here we have outlined a detailed procedure employing a phosphoserine-specific anti-Akt antibody to ex作者: 贊成你 時(shí)間: 2025-3-30 17:40 作者: Emmenagogue 時(shí)間: 2025-3-30 23:20 作者: Triglyceride 時(shí)間: 2025-3-31 02:12 作者: 知識(shí) 時(shí)間: 2025-3-31 06:35 作者: 串通 時(shí)間: 2025-3-31 10:18
Sebastian J. Fleire,Facundo D. Batistang from growth models with perfect competition, the new framework featured forward- looking entrepreneurs. Firms maximize profits intertemporarily, i. e. their in- vestment leads to instantaneous sunk costs and offers flows of future profits. Firms finance this investment by launching shares. The ca作者: Cuisine 時(shí)間: 2025-3-31 15:14
F.-Xabier Contreras,Ana-Victoria Villar,Alicia Alonso,Félix M. Go?iowth models with perfect competition, the new framework featured forward-looking entrepreneurs. Firms maximize profits intertemporarily, i.e. their investment leads to instantaneous sunk costs and offers flows of future profits. Firms finance this investment by launching shares. The capital market i作者: 深淵 時(shí)間: 2025-3-31 20:39 作者: 拱墻 時(shí)間: 2025-4-1 01:10
Byung-Kwon Choi,Yun-Kyung Shin,Eun-Young Lee,Pan-Young Jeong,Yhong-Hee Shim,David J. Chitwood,Young owth models with perfect competition, the new framework featured forward-looking entrepreneurs. Firms maximize profits intertemporarily, i.e. their investment leads to instantaneous sunk costs and offers flows of future profits. Firms finance this investment by launching shares. The capital market i作者: 殘酷的地方 時(shí)間: 2025-4-1 04:53 作者: Pudendal-Nerve 時(shí)間: 2025-4-1 09:48 作者: interior 時(shí)間: 2025-4-1 11:49
