標(biāo)題: Titlebook: Lipid Signaling Protocols; Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho [打印本頁(yè)] 作者: Fatuous 時(shí)間: 2025-3-21 16:24
書目名稱Lipid Signaling Protocols影響因子(影響力)
作者: 粘 時(shí)間: 2025-3-21 20:14
,Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative Hilinositol-5-phosphate 4-kinase α (PI5P4Kα) utilizing the .-myo-di16-PtIns(5)P substrate in quantitative high-throughput screening (qHTS) format using the ADP-Glo? technology to couple the production of ADP to a bioluminescent readout.作者: Physiatrist 時(shí)間: 2025-3-22 00:54 作者: olfction 時(shí)間: 2025-3-22 07:47
Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinasly identified actin cytoskeleton regulators—the WASH complex and RhoGEF1, as major novel interactors of PI4KIIα. While this chapter focuses on the PI4KIIα interactome, this protocol can and has been used to generate other membrane interactome networks.作者: AUGUR 時(shí)間: 2025-3-22 11:03 作者: 性上癮 時(shí)間: 2025-3-22 13:34
,High-Throughput Fluorometric Assay for Membrane–Protein Interaction, rapid, sensitive, and accurate determination of lipid specificity and affinity for various lipid binding domains and proteins, and also high-throughput screening of small molecules that modulate membrane binding of proteins.作者: OTTER 時(shí)間: 2025-3-22 18:08
1064-3745 ation advice from the experts.In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism 作者: 猜忌 時(shí)間: 2025-3-22 23:47
Book 2016Latest editions techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. .Lipid Signaling Protocols, Second Edition. guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the t作者: 大火 時(shí)間: 2025-3-23 03:35
Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity,ll as for the identification of their ligands. In this chapter we describe in detail the assays performed to investigate LXR activity in a macrophage-like cell line (RAW 267.4). These can be easily adapted to other nuclear receptors and transcription factors.作者: micturition 時(shí)間: 2025-3-23 08:40
,Using Surface Plasmon Resonance to Quantitatively Assess Lipid–Protein Interactions,ar interactions and the kinetics and affinity of an interaction. This technique has been especially useful in measuring the affinities of lipid-binding proteins to intact liposomes of varying lipid compositions.作者: 祝賀 時(shí)間: 2025-3-23 11:09
Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]Sol to measure ceramide and SM biosynthesis in cultured cells based on [.H]serine incorporation at the first step in the pathway. The method can be used to assay the effect of pharmacological and genetic manipulations on ceramide synthesis and transport to the Golgi apparatus.作者: deactivate 時(shí)間: 2025-3-23 16:41
Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions,y allows measurement of the relative phosphoinositide composition of each membrane fraction or organelle and determination of the microenvironment in which each species is enriched. Here, we describe the steps to separate and quantify total or localized phosphoinositides from cultured cells.作者: Redundant 時(shí)間: 2025-3-23 18:19 作者: HATCH 時(shí)間: 2025-3-23 23:39
Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors,may change the location of the target molecules. In this chapter, we describe how to examine dynamics of raft-associated glycosylphosphatidylinositol (GPI)-anchored receptors and interactions of the receptors with downstream signaling molecules by single-particle tracking or single-molecule imaging techniques.作者: 斜 時(shí)間: 2025-3-24 03:20
https://doi.org/10.1007/978-1-4939-3170-5cell function; ceramides; cholesterol-rich lipid rafts; detergent-resistant membrane; lipid signaling; me作者: laxative 時(shí)間: 2025-3-24 10:24 作者: Albinism 時(shí)間: 2025-3-24 12:43
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/586856.jpg作者: muster 時(shí)間: 2025-3-24 16:52
Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary lar membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.作者: Mets552 時(shí)間: 2025-3-24 22:01 作者: 發(fā)牢騷 時(shí)間: 2025-3-24 23:32 作者: Repatriate 時(shí)間: 2025-3-25 06:16
rs?nlichen Haftung von Gesch?ftsführern nach dem deutschen GmbHG bzw. dem AktG. Als Direktoren werden nachfolgend die Mitglieder der Gesch?ftsleitung bezeichnet, seien es Gesch?ftsführer, Vorst?nde oder andere, die Gesellschaft nach au?en vertretende, Organe. Die Gründung eines Unternehmens in Thail作者: 我不怕犧牲 時(shí)間: 2025-3-25 09:47
Mindy I. Davis,Atsuo T. Sasaki,Anton Simeonovrs?nlichen Haftung von Gesch?ftsführern nach dem deutschen GmbHG bzw. dem AktG. Als Direktoren werden nachfolgend die Mitglieder der Gesch?ftsleitung bezeichnet, seien es Gesch?ftsführer, Vorst?nde oder andere, die Gesellschaft nach au?en vertretende, Organe. Die Gründung eines Unternehmens in Thail作者: 宏偉 時(shí)間: 2025-3-25 11:41
Xin Ying Lim,Russell Pickford,Anthony S. Donrs?nlichen Haftung von Gesch?ftsführern nach dem deutschen GmbHG bzw. dem AktG. Als Direktoren werden nachfolgend die Mitglieder der Gesch?ftsleitung bezeichnet, seien es Gesch?ftsführer, Vorst?nde oder andere, die Gesellschaft nach au?en vertretende, Organe. Die Gründung eines Unternehmens in Thail作者: 盡管 時(shí)間: 2025-3-25 19:29
Timothy A. Couttas,Anthony S. Donrs?nlichen Haftung von Gesch?ftsführern nach dem deutschen GmbHG bzw. dem AktG. Als Direktoren werden nachfolgend die Mitglieder der Gesch?ftsleitung bezeichnet, seien es Gesch?ftsführer, Vorst?nde oder andere, die Gesellschaft nach au?en vertretende, Organe. Die Gründung eines Unternehmens in Thail作者: collagen 時(shí)間: 2025-3-25 21:11 作者: 責(zé)難 時(shí)間: 2025-3-26 03:03
Joachim Füllekrug Ph.D.,Margarete Poppelreutherrs?nlichen Haftung von Gesch?ftsführern nach dem deutschen GmbHG bzw. dem AktG. Als Direktoren werden nachfolgend die Mitglieder der Gesch?ftsleitung bezeichnet, seien es Gesch?ftsführer, Vorst?nde oder andere, die Gesellschaft nach au?en vertretende, Organe. Die Gründung eines Unternehmens in Thail作者: acrophobia 時(shí)間: 2025-3-26 07:19 作者: 機(jī)械 時(shí)間: 2025-3-26 10:33
Matthew C. Gage,Benoit Pourcet,Inés Pineda-Torran, wirtschaftlichen und rechtlichen Rahmenbedingungen in Tha.Dieses Buch bietet Praktikern einen essenziellen überblick über das aktuelle thail?ndische Wirtschaftsrecht. Thailand geh?rt heute zu den wirtschaftlich attraktivsten Hubs in Südostasien und wirbt als etablierter Wirtschaftsstandort erfolg作者: Project 時(shí)間: 2025-3-26 13:58
Kimberly J. Krager,John G. Kolandvestment process. 25 Basic elements for arithmetical study of selection 29 Thc study of selection from non-numerical elements. 37 2. Programming Investment Activity 49 Classical programming techniques. 49 Handling of an investment programme. 56 Incorporation of costs into investment projects . . . .作者: FECK 時(shí)間: 2025-3-26 18:58
Mitul Sarkar,John G. Kolandvestment process. 25 Basic elements for arithmetical study of selection 29 Thc study of selection from non-numerical elements. 37 2. Programming Investment Activity 49 Classical programming techniques. 49 Handling of an investment programme. 56 Incorporation of costs into investment projects . . . .作者: 輕彈 時(shí)間: 2025-3-26 22:30
Massimo Aureli,Sara Grassi,Sandro Sonnino,Alessandro Prinetti on the investment process. 25 Basic elements for arithmetical study of selection 29 Thc study of selection from non-numerical elements. 37 2. Programming Investment Activity 49 Classical programming techniques. 49 Handling of an investment programme. 56 Incorporation of costs into investment projec作者: Outshine 時(shí)間: 2025-3-27 01:08 作者: Little 時(shí)間: 2025-3-27 06:16
Kathryn Del Vecchio,Robert V. Stahelinchtige über die chinesischen Rahmenbedingungen für erfolgrei.Die VR China geh?rt zu den attraktivsten Investitions- und Absatzm?rkten der Welt. Dennoch birgt die gesch?ftliche Aktivit?t in China für westliche Unternehmen finanzielle und unternehmerische Risiken - ein viel zitiertes Beispiel ist inte作者: Prostatism 時(shí)間: 2025-3-27 11:49
Ricarda A. Busse,Andreea Scacioc,Amanda M. Schalk,Roswitha Krick,Michael Thumm,Karin Kühnelchtige über die chinesischen Rahmenbedingungen für erfolgrei.Die VR China geh?rt zu den attraktivsten Investitions- und Absatzm?rkten der Welt. Dennoch birgt die gesch?ftliche Aktivit?t in China für westliche Unternehmen finanzielle und unternehmerische Risiken - ein viel zitiertes Beispiel ist inte作者: Mendicant 時(shí)間: 2025-3-27 13:46
,Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative His. Distinct lipid kinases regulate the seven different phosphorylated forms of phosphatidylinositol (PtdIns). Some lipid kinases utilize long-chain lipid substrates that have limited solubility in aqueous solutions, which can lead to difficulties in developing a robust and miniaturizable biochemical作者: Recessive 時(shí)間: 2025-3-27 19:42 作者: addition 時(shí)間: 2025-3-28 00:36
Fluorescent Assays for Ceramide Synthase Activity,through their biophysical properties and their specific interactions with cell signaling proteins. Literature on the importance of ceramides in physiology and pathological conditions continues to grow, with ceramides having been identified as central effectors in major human pathologies such as diab作者: 血友病 時(shí)間: 2025-3-28 05:41
Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinasral cell signaling pathways. PI4KIIα is the most abundant membrane-associated kinase in mammalian cells and is involved in a variety of essential cellular functions. However, the precise role(s) of PI4KIIα in the cell is not yet completely deciphered. Here we present an experimental protocol that us作者: LUT 時(shí)間: 2025-3-28 08:50 作者: 工作 時(shí)間: 2025-3-28 13:39
Qualitative and Quantitative In Vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specifhoinositides has been shown in a variety of cellular functions such cell proliferation, cell growth, apoptosis, and vesicle trafficking. Phospholipid phosphatases regulate cell signaling by modifying the concentration of phosphoinositides and their dephosphorylated products. To understand the role o作者: fatty-acids 時(shí)間: 2025-3-28 17:41
Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity,y by transcription factors, and the discovery of gene regulatory elements and small-molecule modulators with high levels of precision. This is made possible through detection of bioluminescence produced by luciferase-coding reporters in a wide range of cellular environments. These assays are routine作者: somnambulism 時(shí)間: 2025-3-28 18:53
Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microoteins, including ion channels, receptors, and other signaling molecules, exhibit a profound submicroscopic spatial organization, in some cases clustering in submicron membrane subdomains having a protein and lipid composition distinct from that of the bulk membrane. In the case of membrane-associat作者: Canopy 時(shí)間: 2025-3-28 22:54 作者: FLACK 時(shí)間: 2025-3-29 06:58
Isolation and Analysis of Detergent-Resistant Membrane Fractions,omains highly enriched in glycosphingolipids, sphingomyelin, ceramide, and cholesterol, was formulated by van Meer and Simons in 1988 and came to a turning point when it was suggested that lipid rafts could be isolated thanks to their resistance to solubilization by some detergents, namely Triton X-作者: 正式通知 時(shí)間: 2025-3-29 07:19
Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor,al functions. A specific marker for this important entity of cellular structure is urgently needed. Thus, we propose in this method chapter that the membrane-bound ER chaperone sigma-1 receptor serves as an ideal marker for the MAM. We describe in detail the preparation and purification of the MAM b作者: NEX 時(shí)間: 2025-3-29 14:44
,Using Surface Plasmon Resonance to Quantitatively Assess Lipid–Protein Interactions,ng a ligand onto a senor chip, measuring a baseline resonance angle, and flowing an analyte in bulk solution over the fixed ligand to measure the subsequent change in resonance angle. The mass of analyte bound to fixed ligand is directly proportional to the resonance angle change and the system is s作者: 刀鋒 時(shí)間: 2025-3-29 18:04
,Analyzing Protein–Phosphoinositide Interactions with Liposome Flotation Assays,ons more than protein–lipid overlay assays, which makes this method less prone to detect false positive interactions. However, liposome lipid composition must be well-considered in order to prevent nonspecific binding of the protein through electrostatic interactions with negatively charged lipids l作者: 有花 時(shí)間: 2025-3-29 22:13 作者: Pantry 時(shí)間: 2025-3-30 03:34 作者: forager 時(shí)間: 2025-3-30 07:21
Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]Ss and transporters. The biosynthesis of sphingolipids in cultured cells is initiated in the endoplasmic reticulum (ER) by the formation of a sphingoid base from serine and palmitoyl-CoA. N-acylation of the sphingoid base produces ceramide, which is transported to the Golgi apparatus where phosphocho作者: Innovative 時(shí)間: 2025-3-30 10:05
Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary lar membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.作者: 分貝 時(shí)間: 2025-3-30 13:22
Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions,nt for vesicle trafficking and organelle function (Di Paolo and De Camilli, Nature 443(7112):651–657, 2006). The response triggered by these lipids is heavily dependent on the microenvironment in which they are found. HPLC analysis of labeled phosphoinositides allows quantification of the levels of 作者: 省略 時(shí)間: 2025-3-30 20:21 作者: ROOF 時(shí)間: 2025-3-30 20:50 作者: TATE 時(shí)間: 2025-3-31 02:37 作者: 控制 時(shí)間: 2025-3-31 05:23
Qualitative and Quantitative In Vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specifoduce the necessary yield and functionality in order to further characterize these enzymes. The outstanding versatility and sensitivity of this assay system are yet unmatched and are therefore currently considered the standard of the field.作者: cylinder 時(shí)間: 2025-3-31 09:36
Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Micro-rich lipid raft structures. However, the intrinsically perturbing nature of fractionation methods makes the interpretation of such characterization subject to question, and indeed the existence and significance of lipid rafts remain controversial. Electron microscopic (EM) imaging of immunogold-lab作者: narcotic 時(shí)間: 2025-3-31 15:38 作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-31 18:21
Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging,ic lipid binding domains (LBDs) fused to fluorescent proteins. We discuss how to design such sensors, including the criteria for selecting the lipid binding domains of interest and to validate them. We also emphasize the care that must be taken during data analysis as well as the main limitations an作者: 裙帶關(guān)系 時(shí)間: 2025-4-1 00:43 作者: 教唆 時(shí)間: 2025-4-1 05:40
Kathryn Del Vecchio,Robert V. Stahelin nach China und ?eignet sich zugleich als Nachschlagewerk für bereits im Land ans?ssige westliche Unternehmen. Die zweite Auflage wurde gründlich aktualisiert und um ein Kapitel zum neuen Markengesetz erweitert, das seit Mai 2014 in Kraft ist..978-3-658-05058-0978-3-658-05059-7作者: Limerick 時(shí)間: 2025-4-1 06:34
Ricarda A. Busse,Andreea Scacioc,Amanda M. Schalk,Roswitha Krick,Michael Thumm,Karin Kühnel nach China und ?eignet sich zugleich als Nachschlagewerk für bereits im Land ans?ssige westliche Unternehmen. Die zweite Auflage wurde gründlich aktualisiert und um ein Kapitel zum neuen Markengesetz erweitert, das seit Mai 2014 in Kraft ist..978-3-658-05058-0978-3-658-05059-7作者: PURG 時(shí)間: 2025-4-1 12:24
Gesellschaft haften. Nach Sec.?1096 des Civil and Commercial Code (CCC) ist die Haftung der Anteilseigner auf die H?he ihres Gesellschaftsanteils beschr?nkt. Unternehmen werden in Thailand vom Board of Directors geführt, welche vom Statutory Meeting der Unternehmensgründer gem?? Sec.?1107 (6) CCC ge作者: abolish 時(shí)間: 2025-4-1 16:05
