標題: Titlebook: Leukemia Stem Cells; Methods and Protocol César Cobaleda,Isidro Sánchez-García Book 2021 Springer Science+Business Media, LLC, part of Spri [打印本頁] 作者: 怕跛行他有限 時間: 2025-3-21 17:25
書目名稱Leukemia Stem Cells影響因子(影響力)
書目名稱Leukemia Stem Cells影響因子(影響力)學科排名
書目名稱Leukemia Stem Cells網(wǎng)絡公開度
書目名稱Leukemia Stem Cells網(wǎng)絡公開度學科排名
書目名稱Leukemia Stem Cells被引頻次
書目名稱Leukemia Stem Cells被引頻次學科排名
書目名稱Leukemia Stem Cells年度引用
書目名稱Leukemia Stem Cells年度引用學科排名
書目名稱Leukemia Stem Cells讀者反饋
書目名稱Leukemia Stem Cells讀者反饋學科排名
作者: 歌唱隊 時間: 2025-3-21 21:01
Book 2021eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and comprehensive, .Leukemia Stem Cells: Methods and Protocols. serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research..作者: oncologist 時間: 2025-3-22 02:48 作者: 使入迷 時間: 2025-3-22 05:14
Introduction and Classification of Leukemiasess in how we describe the nature of the “target” cells that genetic insults transform to give rise to the hematological malignancies. Here I describe how both longstanding and new information has influenced classifying, for diagnosis, the hematological malignancies.作者: Occupation 時間: 2025-3-22 12:17
PCR Technology to Identify Minimal Residual Diseaseedure which has been developed and standardized (with minor modification in single labs) through the last 20?years of activity of the EuroMRD Consortium, a volunteer activity of expert laboratories that is continuously providing education, standardization, quality control rounds, and guidelines for interpretation of RQ-PCR data.作者: adjacent 時間: 2025-3-22 13:17
Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignanciesregulation. So far, only one standardized academic protocol for this task has been published, developed, and validated within the EuroClonality-NGS working group. In this chapter we describe the materials and methods for amplicon library preparation for sequencing on Illumina MiSeq, and the bioinformatic pipeline for this protocol.作者: 休息 時間: 2025-3-22 20:56
Leukemic Stem Cell Culture in Cytokine-Free Mediumant after culture. This impedes development of new therapies for AML that target LSCs. Here, we present a simple strategy to culture LSCs in cytokine-free medium and to perform flow cytometric analysis of the resulting cell population for the characterization of LSCs maintenance and differentiation.作者: 大暴雨 時間: 2025-3-22 22:05
Book 2021and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the high作者: outer-ear 時間: 2025-3-23 04:57 作者: Inculcate 時間: 2025-3-23 08:38 作者: 反抗者 時間: 2025-3-23 10:19
In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomicse profiling to single mammalian cells. Here, we describe specific techniques that increase the sensitivity of single-cell proteomics by orders of magnitude, enabling the promise of single-cell proteomics to become a reality. We anticipate such techniques can significantly advance the understanding of leukemic stem cells.作者: 事物的方面 時間: 2025-3-23 14:58
In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantationtanding of aged HSC differentiation potential to all five blood lineages. In this chapter, we describe a method to perform in vivo clonal analysis of aged HSCs using single-cell transplantation, incorporating a five-blood lineage tracing system using the Kusabira-Orange (KuO) trancsgenic mouse line.作者: 散布 時間: 2025-3-23 19:14
Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acidumbers of functional HSCs and committed progenitors (HPCs). The expanded HSCs are capable of establishing both short-term and long-term multilineage hematopoietic reconstitution. This highly reproducible and simple protocol can be also applied to expansion of both HSCs and HPCs from different sources including the bone marrow and peripheral blood.作者: browbeat 時間: 2025-3-24 00:53 作者: 吸氣 時間: 2025-3-24 04:40
ng and sharing of lots of information (regarding vehicles, roads and their surroundings) through the Internet and hence, helps in making intelligent decisions. On the other hand, Software Defined Networking (SDN) has the capability of designing a flexible programmable IoV network that can foster inn作者: 混合物 時間: 2025-3-24 08:08 作者: 極大的痛苦 時間: 2025-3-24 10:47 作者: 鐵砧 時間: 2025-3-24 16:24 作者: Diskectomy 時間: 2025-3-24 19:16 作者: Highbrow 時間: 2025-3-24 23:52
component of DSSC has been an improvement to obtain a satisfactory efficiency. DSSC consists of three principal layers, two of them act as load carriers and the other keeps the electrolyte responsible for producing a charge flow to the electrodes of the solar cell. In this work, the intermediate lay作者: 并入 時間: 2025-3-25 05:36 作者: AIL 時間: 2025-3-25 10:25 作者: 脆弱么 時間: 2025-3-25 15:19
Leukemia Stem Cells: Concept and Implicationsarly demonstrated, all the consequences of their existence are far from being realized neither in the clinic nor, very often, in basic and translational cancer research. The existence of CSCs supposes a true change of paradigm in our understanding of cancer, but it will only have a real impact when 作者: Onerous 時間: 2025-3-25 17:57 作者: 相反放置 時間: 2025-3-25 23:52 作者: Obstacle 時間: 2025-3-26 01:12
Mass Cytometry of Hematopoietic Cellsn single cells. Analytes are detected via antibodies tagged with heavy metal and detected by using a time-of-flight mass spectrometer. Over the past decade, mass cytometry has proven to be a valuable method for immunophenotyping hematopoietic cells with remarkable precision in both healthy and malig作者: 物種起源 時間: 2025-3-26 06:28 作者: semble 時間: 2025-3-26 12:06 作者: BLAND 時間: 2025-3-26 13:59 作者: 技術(shù) 時間: 2025-3-26 17:28
Single-Cell Transcriptomic Analysis of Hematopoietic Cellsrs, scRNA-Seq contributed to the progress of the hematology field, advancing our knowledge of both normal and malignant hematopoiesis. Different scRNA-Seq methods are available, all relying on the conversion of RNA to cDNA, followed by amplification of cDNA in order to obtain a sufficient amount of 作者: harbinger 時間: 2025-3-27 00:42 作者: 切割 時間: 2025-3-27 01:36 作者: 暫時別動 時間: 2025-3-27 06:05
Experimental Competitive Bone Marrow Transplant Assaysrrow cells from a healthy donor to the patient is an efficient solution to restore normal hematopoiesis. Bone marrow transplant in a mouse model is often used to study HSC function and capacity to repopulate an irradiated recipient. This protocol details the different steps of a competitive bone mar作者: 含鐵 時間: 2025-3-27 09:43 作者: 灰心喪氣 時間: 2025-3-27 16:43 作者: Pcos971 時間: 2025-3-27 18:01
Leukemic Stem Cell Culture in Cytokine-Free Medium are believed to be a major cause of relapse in acute myeloid leukemia (AML). Despite the aggressive characteristics of acute leukemia, AML blasts are difficult to culture once removed from the patient, and LSCs, which are a minor fraction of the blast population, are especially difficult to transpl作者: forestry 時間: 2025-3-27 22:43
Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acidtransplantation but lack a matched donor. However, the limited number of HSCs within each UCB unit remains a major challenge for their use in regenerative medicine and HSC transplantation in adults. Efficient expansion of human HSCs in ex vivo cultures initiated with CD34. cells isolated from UCBs c作者: 方舟 時間: 2025-3-28 04:57
Isolation, Culture, and Manipulation of Human Cord Blood ProgenitorsThese characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vi作者: 滋養(yǎng) 時間: 2025-3-28 07:10
César Cobaleda,Isidro Sánchez-GarcíaIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: HAUNT 時間: 2025-3-28 12:41
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/585300.jpg作者: garrulous 時間: 2025-3-28 14:38
978-1-0716-0812-8Springer Science+Business Media, LLC, part of Springer Nature 2021作者: CLIFF 時間: 2025-3-28 19:59
Leukemia Stem Cells978-1-0716-0810-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Blazon 時間: 2025-3-28 23:47
Analysis and Isolation of Mouse Leukemic Stem CellsHSCs) and leukemic stem cells (LSCs) can be highly purified by flow cytometry. Isolated HSCs and LSCs can be functionally identified by transplantation assays and can also be studied at the molecular level. Here we describe the flow cytometry methods for analysis and isolation of mouse HSCs and LSCs.作者: maculated 時間: 2025-3-29 05:16
Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)ukemic cells in the bone marrow. Most commonly, reverse transcriptase PCR is used to detect these translocations on RNA level. This technique has severe drawbacks, including sensitivity to contamination and instability of RNA. Here, we describe the . (GIPFEL) that overcomes these pitfalls.作者: 不在灌木叢中 時間: 2025-3-29 11:03
Isolation, Culture, and Manipulation of Human Cord Blood ProgenitorsThese characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vitro assays in order to study leukemic processes.作者: Ambulatory 時間: 2025-3-29 13:20
https://doi.org/10.1007/978-1-0716-0810-4Cancer stem cells; Hematopoietic stem cells; Tissue culture; LSCs; Human leukemias; Animal models作者: 危機 時間: 2025-3-29 19:34 作者: 刺穿 時間: 2025-3-29 20:40 作者: NEG 時間: 2025-3-30 00:36
Geoffrey Brownle or everyday language, common situations, requiring the use of arithmetic, geometry, or logic, for its solution. In dialogues, a procedure was developed to make decisions, based on answers and a record (knowledge base) of the occurrences of omission, while maintaining the communication process. Th作者: Expressly 時間: 2025-3-30 07:28
Isidro Sánchez-García,César Cobaleda results show that 45% of users remain with the same provider for more than 12?months. It is concluded that the virtual assistant strategy reaches an acceptance rate of 71% and generates the beginning of customer loyalty for after-sales services in fixed Internet companies.作者: 首創(chuàng)精神 時間: 2025-3-30 11:37 作者: outskirts 時間: 2025-3-30 14:47
lm was anatase phase and the particle size was nanometric and submicron respectively, while the characterization of the porous film consisted of the determination of the crystalline structure, morphological characteristics, and absorbance capacity. The results proved that the TiO. films built with M作者: Obstacle 時間: 2025-3-30 17:49
Fang Dong,Haitao Bai,Hideo Emaame measurement parameters, achieving similarity percentages of 78% for pelvic rotation as well as 53 and 79% for pelvic obliquity. Results suggest that is essential to extend the age range of the children, as well as to increase the numbers of participants evaluated to establish a follow-up in the 作者: 群島 時間: 2025-3-30 20:59 作者: 臭了生氣 時間: 2025-3-31 02:25 作者: infinite 時間: 2025-3-31 06:38
An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemmplex to achieve. To overcome these limitations, model-driven approaches can be exploited to investigate different aspects of cell population dynamics. Among the existing approaches, the ProCell framework is able to perform detailed and accurate stochastic simulations of cell proliferation, relying 作者: Grievance 時間: 2025-3-31 12:29 作者: Initiative 時間: 2025-3-31 14:39
Giovanni Cazzaniga,Simona Songia,Andrea Biondi,On Behalf of the EuroMRD Working Group作者: Diaphragm 時間: 2025-3-31 18:44 作者: 同時發(fā)生 時間: 2025-3-31 23:02
Roxann Hétu-Arbour,Sarah Bouali,Krista M. Heinonen
