標(biāo)題: Titlebook: Leptospira spp.; Methods and Protocol Nobuo Koizumi,Mathieu Picardeau Book 2020 Springer Science+Business Media, LLC, part of Springer Natu [打印本頁] 作者: deep-sleep 時間: 2025-3-21 16:26
書目名稱Leptospira spp.影響因子(影響力)
書目名稱Leptospira spp.影響因子(影響力)學(xué)科排名
書目名稱Leptospira spp.網(wǎng)絡(luò)公開度
書目名稱Leptospira spp.網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Leptospira spp.被引頻次
書目名稱Leptospira spp.被引頻次學(xué)科排名
書目名稱Leptospira spp.年度引用
書目名稱Leptospira spp.年度引用學(xué)科排名
書目名稱Leptospira spp.讀者反饋
書目名稱Leptospira spp.讀者反饋學(xué)科排名
作者: 棲息地 時間: 2025-3-21 20:29 作者: languid 時間: 2025-3-22 04:04
Célia Fontana,Jean Reyeshow important limitations, mainly in the assessment of the effects on the global industrial risk due to the contemporary presence of different risk sources in a narrow area. Therefore, the application of QARA techniques to land use planning in the framework of “Seveso-II” Directive requires the furt作者: Ejaculate 時間: 2025-3-22 08:01 作者: Directed 時間: 2025-3-22 09:25
Olivier Schiettekatte,Pascale Bourhy months, and prices will fall, and this will have a negative impact on farmers and the agricultural sector. Policy makers and managerial staff from all over the globe must pay attention and make the most suitable steps so that they do not transform the current health crisis into a completely regrett作者: 密碼 時間: 2025-3-22 15:29 作者: penance 時間: 2025-3-22 18:54 作者: Grievance 時間: 2025-3-22 22:45 作者: 闖入 時間: 2025-3-23 01:22 作者: 支柱 時間: 2025-3-23 08:12 作者: jumble 時間: 2025-3-23 14:18
In Vivo Imaging of Bioluminescent Leptospires,This useful and accessible technology facilitates the study of the kinetics of blood and tissue dissemination of live leptospires, and the pharmacological impact of treatments and host directed therapeutics.作者: Factorable 時間: 2025-3-23 19:02 作者: Feckless 時間: 2025-3-23 23:37 作者: conjunctivitis 時間: 2025-3-24 03:56
Nobuo Koizumi,Mathieu PicardeauIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: precede 時間: 2025-3-24 06:30
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/585006.jpg作者: 止痛藥 時間: 2025-3-24 13:05 作者: overbearing 時間: 2025-3-24 15:13 作者: 預(yù)防注射 時間: 2025-3-24 21:04
Isolation and Culture of , from Clinical and Environmental Samples, leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.作者: 去世 時間: 2025-3-25 03:02
Isolation, Purification, and Characterization of Leptophages,he genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.作者: 詢問 時間: 2025-3-25 04:29 作者: 正面 時間: 2025-3-25 09:18 作者: 口訣 時間: 2025-3-25 15:33
Cell Monolayer Translocation Assay,mmune response, disseminate, and establish infection. Cell monolayer translocation assay allows for the quantification of . strain’s competence to cross cell barriers while measuring the integrity of the polarized eukaryotic cell monolayer during this process.作者: Fibroid 時間: 2025-3-25 19:52
Cell Enumeration of , by Flow Cytometry,croscope, but this method remains time-consuming and lacks reproducibility. New alternative techniques are then of great interest. Here we describe the protocol for counting leptospires by flow cytometry. This method is rapid, reproducible, sensitive, and hence suitable to become a new standard to enumerate . spp.作者: 圓桶 時間: 2025-3-25 22:15 作者: Biofeedback 時間: 2025-3-26 03:45 作者: 借喻 時間: 2025-3-26 06:42 作者: grenade 時間: 2025-3-26 08:42
The Single-Step Method of RNA Purification Applied to ,erful ability of guanidinium thiocyanate to inactivate RNases and on the different solubilities of RNA and DNA in acidic phenol. This method allows one to reproducibly obtain total RNAs with high yield and integrity, as determined by capillary electrophoresis, suitable for the RNA sequencing technology.作者: 嚴(yán)厲譴責(zé) 時間: 2025-3-26 15:40 作者: 無政府主義者 時間: 2025-3-26 19:01 作者: Substance 時間: 2025-3-26 21:26
In Situ Structural Analysis of , spp. by Electron Cryotomography,ation, data collection, tilt series alignment, and tomographic reconstruction for visualization of intact periplasmic flagella in . spp. This protocol is capable of determining protein structures at resolutions high enough to visualize their individual domains and secondary structures in their cellular environment.作者: 有花 時間: 2025-3-27 04:26 作者: 信條 時間: 2025-3-27 08:38 作者: 褲子 時間: 2025-3-27 10:38 作者: 不能根除 時間: 2025-3-27 14:58 作者: Odyssey 時間: 2025-3-27 21:26
Core Genome Multi-locus Sequence Typing Analyses of , spp. Using the Bacterial Isolate Genome Sequeies, clades, clonal groups, and sequence types. We show several examples how the cgMLST . database, which is a publicly available web-based database, can be used for the analyses of WGS data of . isolates. This effort was undertaken in order to facilitate international collaborations and support the global surveillance of leptospirosis.作者: hermetic 時間: 2025-3-27 22:58
1064-3745 ation advice from the experts.This volume looks at all aspects of manipulation of .Leptospira spp.. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from b作者: BATE 時間: 2025-3-28 03:08 作者: 發(fā)誓放棄 時間: 2025-3-28 06:28 作者: Mendacious 時間: 2025-3-28 10:31 作者: 學(xué)術(shù)討論會 時間: 2025-3-28 15:08
Célia Fontana,Jean Reyess in the safety requirements of process plants, that have a relevant impact on risk management. Among these are the inclusion of substances likely to be formed in the loss of control of chemical processes in site inventory, the evaluation of domino accident hazard, and the requirement of land-use pl作者: compassion 時間: 2025-3-28 18:49 作者: 混合,攙雜 時間: 2025-3-28 23:48 作者: 欲望 時間: 2025-3-29 07:09 作者: saturated-fat 時間: 2025-3-29 07:32
Christopher J. Pappas,Hui Xu,Md A. Motalebdrastically in recent history. Currently, COVID-19 pandemic (respiratory illness) caused by SARS-CoV-2 virus ravaging fright around the world. The infectious nature of the disease affected the global and local economies and societies. Nations are forced to implement precautionary measure such as res作者: Pituitary-Gland 時間: 2025-3-29 11:23 作者: fallible 時間: 2025-3-29 17:25 作者: 線 時間: 2025-3-29 22:27 作者: 斜坡 時間: 2025-3-30 03:28
Use of MALDI-ToF Mass Spectrometry for Identification of ,lied to identify leptospires at the species level. In the recent years, matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry (MS) has emerged as new tool for the identification of bacterial species in the medical microbiology laboratory. This technology has rapidl作者: cavity 時間: 2025-3-30 07:29
Cell Enumeration of , by Flow Cytometry,motile, and slow-growing bacteria. The current gold standard for cell enumeration is the use of a Petroff-Hausser counting chamber and a dark-field microscope, but this method remains time-consuming and lacks reproducibility. New alternative techniques are then of great interest. Here we describe th作者: 微不足道 時間: 2025-3-30 11:12 作者: Jogging 時間: 2025-3-30 13:01
Purification of LPS from ,to extract and/or purify the leptospiral lipopolysaccharides: the rapid proteinase K method and the classical hot water/phenol extraction. The first method allows to get a quick overview of the LPS O antigen structure, whereas the second method is fitted to study the immunological properties of the 作者: dissent 時間: 2025-3-30 17:57 作者: Infiltrate 時間: 2025-3-30 21:02 作者: 疏忽 時間: 2025-3-31 02:35 作者: 薄膜 時間: 2025-3-31 06:38 作者: 察覺 時間: 2025-3-31 11:26
spp. Toolbox for Chemotaxis Assay,e methods allow to demonstrate chemotaxis defect, identify diverse chemoattractants, or decipher motile behavior quantitatively. These experiments have demonstrated a role of motility and potentially chemotaxis in leptospirosis pathogenesis. We describe extensively the methods and provide the key st作者: Outmoded 時間: 2025-3-31 17:21
In Situ Structural Analysis of , spp. by Electron Cryotomography, are present inside the cell. Electron cryotomography (ECT) is an imaging technique that directly provides three-dimensional (3D) structures of cells and molecular complexes in their cellular environment at nanometer resolution. Here, I present a general protocol of ECT that covers the sample prepar作者: Blanch 時間: 2025-3-31 20:47
Measurement of the Cell-Body Rotation of ,y motors (flagellar motors) and transform the ends of the cell body into spiral or hook shape. The rotations of the periplasmic flagella are thought to gyrate the cell body and rotate the protoplasmic cylinder for propelling the cell; however, the motility mechanism has not been fully elucidated. Si作者: 去世 時間: 2025-3-31 22:45
In Vivo Imaging of Bioluminescent Leptospires, in vivo analysis. The use of pathogens, genetically modified to express with luminescent enzymes, combined to charge-coupled device (CCD) cameras, constitutes a major technological advance for assessing the course of infection in an intact, living host in real time and in a noninvasive way. This te作者: 榨取 時間: 2025-4-1 02:33 作者: 泥瓦匠 時間: 2025-4-1 07:05 作者: 尊敬 時間: 2025-4-1 14:08 作者: 事情 時間: 2025-4-1 16:10
Evaluation of Intracellular Trafficking in Macrophages,e pathogen is formed. This phagosome undergoes a maturation process to acquire an increasingly antimicrobial environment. Leptospiral uptake by macrophages induces the formation of a .-containing phagosome (LCP). The kinetics of lysosomal marker recruitment by the LCP is correlated with virulence. T作者: Constrain 時間: 2025-4-1 19:29 作者: 不能妥協(xié) 時間: 2025-4-2 00:01 作者: filicide 時間: 2025-4-2 03:38
,Einleitung: Die wachsende Bedeutung der Internet-?konomie,In diesem einführenden Kapitel erhalten Sie eine Vorstellung von der Internet-?konomie. Sie erfahren insbesondere,
