作者: 重畫只能放棄 時間: 2025-3-21 23:16
Detection of Replication-Competent Lentiviral Particless have been proposed to detect RCL, all of which report high specificity and sensitivity and all of which can discriminate between replication-defective virus and RCL. There is currently a compelling need for standardization and validation of this method.作者: Vldl379 時間: 2025-3-22 00:56 作者: Nostalgia 時間: 2025-3-22 07:34 作者: Infelicity 時間: 2025-3-22 09:45 作者: 法律 時間: 2025-3-22 16:07 作者: 放逐 時間: 2025-3-22 17:28
Mesenchymal Stem Cellstheir ability of self-renewal and their potential to differentiate into terminal osteocytes, chondrocytes, myocytes, tenocytes, adipocytes, and neural cells in vivo and in vitro (.–.), bone marrow-derived MSCs have attracted considerable attention as potential tools for therapeutic gene transfer.作者: NEXUS 時間: 2025-3-22 23:14
Lentivirus Gene Engineering Protocols978-1-59259-393-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 情節(jié)劇 時間: 2025-3-23 04:57
Maurizio FedericoIncludes supplementary material: 作者: SIT 時間: 2025-3-23 05:42 作者: 異端邪說2 時間: 2025-3-23 13:26 作者: 熱心 時間: 2025-3-23 14:35
ith respect to properties, but does not scale; the latter does scale but cannot guarantee the absent of errors. In the authors’ previous work, a mechanism of statically analysing a model has been used to build an abstraction of the original model, which in turn is used to guide a heuristic search in作者: 諷刺 時間: 2025-3-23 19:18 作者: TAP 時間: 2025-3-24 00:55 作者: ostrish 時間: 2025-3-24 02:27 作者: 牛的細(xì)微差別 時間: 2025-3-24 07:39
Michael P. Marino,Milson J. Luce,Jakob Reiserthmetic and potentially uncountable state spaces. Our approach integrates methods from symbolic computation, probability theory, and static analysis in order to automatically capture sensitivity information about probabilistic loops. Sensitivity information allows us to formally establish how value 作者: RUPT 時間: 2025-3-24 13:18
Joel Gatlin,Miguel Islas-Ohlmayer,J. Victor Garciah complexity of their codebases, which makes bug occurrence disturbingly frequent. Tool competitions have showcased many examples of state-of-the-art solvers disagreeing on the satisfiability of logic formulas, be them solvers for Boolean satisfiability (SAT), satisfiability modulo theories (SMT), o作者: atopic 時間: 2025-3-24 18:03
Yan Cui,Lung-Ji Changquence of actions with incomplete information about the underlying state of the system. As such, methods that can act in the presence of incomplete state information are of special interest to machine learning, planning, and control communities. In the scope of this paper, we consider environments t作者: 五行打油詩 時間: 2025-3-24 21:46 作者: defendant 時間: 2025-3-25 02:50 作者: Pepsin 時間: 2025-3-25 07:22 作者: 事情 時間: 2025-3-25 09:06 作者: cavity 時間: 2025-3-25 14:12
Jay L. Nadeauvironment for graphically constructing invariant-based programs (as statechart-like diagrams) and verifying their correctness (by invoking an automatic theorem prover). It borrows the specification language, logical framework and proof tactics from the PVS system. In this paper, we describe an exten作者: 卜聞 時間: 2025-3-25 16:34
Jane Fleming,Samantha L. Ginn,Ian E. Alexanderible impact on user experience. This paper outlines the application of a process for development of a run-time management system that integrates a range of modelling, validation, verification and generation tools at appropriate stages. We outline the models, process and tools we used to develop a te作者: 巧辦法 時間: 2025-3-25 19:58
Dèsirèe Bonci,Michael V. G. Latronico,Gianluigi Condorellites user-defined taint analyses that are formally specified in a domain-specific language for expressing taint-flow analyses. We demonstrate how dynamic taint analysis can be integrated into ., a dynamic symbolic execution engine for the . virtual machine in . PathFinder. The integration of the two 作者: 善辯 時間: 2025-3-26 02:09 作者: 剛毅 時間: 2025-3-26 07:27 作者: Accrue 時間: 2025-3-26 11:42 作者: 疲勞 時間: 2025-3-26 14:09
From Lentiviruses to Lentivirus Vectorse 20th century (.), lentiviruses were considered as rather mysterious viruses until the isolation of the human immunodeficiency virus type 1 (HIV-1) occurred at the beginning of 1980s. Lentiviruses are enveloped viruses carrying two copies of single-strand positive (i.e., codifying) RNA and are cons作者: MERIT 時間: 2025-3-26 17:46
The Choice of a Suitable Lentivirus Vectorvivo (reviewed in ..–.). Lentiviral vectors are particularly efficient in transducing multipotent stem cells, such as hematopoietic stem cells (HSC), without compromising their self-renewing and organ repopulation capacity upon transplantation in vivo (.–.). This is a crucial advantage over oncoretr作者: 輕信 時間: 2025-3-26 22:39 作者: Scintillations 時間: 2025-3-27 03:29
Small- to Large-Scale Production of Lentivirus Vectorsiginally established for the production of retroviral vectors based on Moloney murine leukemia (MoMLV) virus (.). Simian virus 40 (SV40)-transformed African green monkey kidney (COS-7) cells (.) and human TE671 rhabdomyosarcoma cells (.) have also been used to generate human immunodeficiency virus t作者: MOAN 時間: 2025-3-27 07:38
Detection and Titration of Lentivirus Vector Preparationstential gene transfer applications for any viral vector of interest. This accuracy is especially critical if anticipated clinical applications will require scale-up of vector production or if multiple different vector stocks will be required for serial transductions of target cell populations over t作者: 砍伐 時間: 2025-3-27 11:07 作者: famine 時間: 2025-3-27 17:13
Detection of Replication-Competent Lentiviral Particles but have also raised safety concerns because of potential creation of replication-competent lentivirus (RCL) by uncontrolled recombination (.–.). In order to be safe for clinical use, vector preparations must be formally and extensively tested to show they are absolutely free of RCL. Various method作者: 未成熟 時間: 2025-3-27 18:28 作者: 重力 時間: 2025-3-27 23:31 作者: cochlea 時間: 2025-3-28 04:48 作者: 無節(jié)奏 時間: 2025-3-28 07:00 作者: 粘連 時間: 2025-3-28 12:55 作者: 疲勞 時間: 2025-3-28 15:14 作者: Flounder 時間: 2025-3-28 22:10
Cardiomyocytes in cardiomyocytes. They can be useful for gene reporter studies, but are of little value when a gene needs to be introduced into a large number of cardiac cells. Therefore, viral vectors are used to achieve this goal. Type 5 adenoviruses work well in cardiomyocytes, but they are hard to produce, an作者: Fabric 時間: 2025-3-28 23:46
Airway Epitheliais transmembrane conductance regulator gene (CFTR) that lead to abnormal secretions, recurrent infection and inflammation, bronchiectasis, and premature death. Because airways disease is the major cause of morbidity and mortality in cystic fibrosis, gene therapy efforts have focused on luminal deliv作者: 漫不經(jīng)心 時間: 2025-3-29 04:17
Corneal Cellsnsduction of the cornea is useful for studying the efficacy of gene products on inhibition of corneal neovascularization (.), amelioration of corneal inflammatory disease, or promotion of corneal wound healing in animal models. Ex vivo transduction can also be used for transfer of genes into corneal作者: 致命 時間: 2025-3-29 10:43
Retinal Tissueof inherited retinal degenerations (.). Retinal degenerative disorders are highly heterogeneous, both genetically and phenotypically (.). Different mutations within a single gene (such as rhodopsin) can not only result in different modes of inheritance (autosomal dominant as well as recessive) of th作者: 漫步 時間: 2025-3-29 11:42 作者: legislate 時間: 2025-3-29 17:28 作者: Lyme-disease 時間: 2025-3-29 22:46
The Choice of a Suitable Lentivirus Vector of HIV structural and regulatory sequences in both the transfer and the packaging constructs and have virtually abolished the safety concerns originally raised by the idea of transducing human cells with a derivative of HIV (reviewed in ..). These vectors are now a very promising alternative to tra作者: 確認(rèn) 時間: 2025-3-30 03:49
Small- to Large-Scale Production of Lentivirus Vectorsent cotransfection of COS-7 cells with this .-deleted vector and a gp160 expression vector resulted in packaging of the defective HIV-gpt genome into infectious virions. Upon infection of susceptible cells, the . drug resistance gene was transmitted and expressed, allowing transduced cells to be sel作者: Badger 時間: 2025-3-30 06:36
Detection and Titration of Lentivirus Vector Preparations be conducted based on the number of vector particles added per target cell and will therefore be standardized yielding more reproducible results. The numerical relationship between vector particles utilized per single target cell is designated as the multiplicity of infection (MOI). Establishing an作者: 鐵塔等 時間: 2025-3-30 09:56 作者: CLAP 時間: 2025-3-30 15:21
Lymphocytes/CD8 ratios and T-cell receptor (TCR) repertoire composition of T lymphocytes and induce changes of their cytokine secretion profile, thus precluding preservation of the pool of na?ve lymphocytes and their functional integrity (.,.).作者: 配偶 時間: 2025-3-30 19:22
Monocyte/Macrophages and Dendritic Cellsstate and antigen capture properties. Hence, DCs either induce the appropriate immune response to pathogens or prevent autoimmune reactivity, and could thus be useful for manipulating the immune system by cellular immunotherapy.作者: 極微小 時間: 2025-3-30 21:51
Hippocampal Neuronsve overexpression. We have found lentivirus impractical for acute slice recordings, as the preparations do not live long enough to demonstrate visible expression of reporter genes (e.g., green fluorescent protein [GFP]); for these cultures, AAV or SFV is preferable. In organotypic and dissociated cu作者: 傲慢物 時間: 2025-3-31 02:48 作者: 善變 時間: 2025-3-31 05:46 作者: Insubordinate 時間: 2025-3-31 09:31
Airway Epithelian and production have improved titers, and the development of human and animal lentiviruses may help overcome the requirement for cell proliferation. These developments have raised hopes for retroviral approaches for treatment of CF lung disease.作者: penance 時間: 2025-3-31 14:48 作者: EVADE 時間: 2025-3-31 20:51
Book 20031st editionthat so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical 作者: ectropion 時間: 2025-4-1 00:49 作者: Frenetic 時間: 2025-4-1 05:11 作者: 草本植物 時間: 2025-4-1 09:51
Maurizio Federicoes (more concretely, (un)reachability properties) into account to determine which changes may cause regression bugs where. Our extensive evaluation confirms that for all considered conditional model checkers our proposed difference detector improves the effectiveness and efficiency of difference ver
