作者: Kernel 時(shí)間: 2025-3-21 22:55
Isabelle VernosIncludes supplementary material: 作者: PHON 時(shí)間: 2025-3-22 02:59 作者: 使聲音降低 時(shí)間: 2025-3-22 07:37
Purification of Kinesin from the Brain,The kinesin superfamily constitutes a structurally diverse group of microtubule-based motor proteins that use the energy of Mg-ATP hydrolysis to transport organelles along microtubules (.). Purification of kinesins is required for both the characterization of their motile properties and studies of their regulation.作者: defuse 時(shí)間: 2025-3-22 10:59
Plasmids for Expression of Chimeric and Truncated Kinesin Proteins,The construction of mutant or chimeric proteins enables researchers to identify regions of a protein that are involved in a particular function. The technique of overlap polymerase chain reaction (PCR) (.) allows one to make a number of mutants in a timely fashion.作者: Chemotherapy 時(shí)間: 2025-3-22 14:11 作者: 遺留之物 時(shí)間: 2025-3-22 19:20 作者: 變態(tài) 時(shí)間: 2025-3-22 23:11 作者: 虛構(gòu)的東西 時(shí)間: 2025-3-23 02:32
The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindlghly dynamic bipolar structure assembles and disassembles periodically and its main structural components are microtubules (MTs) and chromosomes. Microtubule-dependent motors in particular members of the kinesin superfamily play an important role in directing the self-organization of microtubules into a bipolar spindle.作者: Relinquish 時(shí)間: 2025-3-23 08:10
Identification of Kinesin-Associated Proteins,re hypothesized to provide regulation of the vesicle-binding and the ATPase activities of the kinesin heavy chain, effectively regulating kinesin-mediated vesicle motility. Thus, the identification of these kinesin-associated proteins would greatly increase our understanding of how kinesin function is regulated in vivo.作者: 祖先 時(shí)間: 2025-3-23 21:48
Crystallization of Kinesin,y chain can be divided into three separate domains: (.) an N-terminal head, or motor domain, (.) an elongated a-helical stretch forming a coiled coil with a second kinesin heavy-chain polypeptide, (.) a C-terminal globular domain responsible for cargo binding and regulation of the motor’s activity (for reviews, ...–.).作者: 旁觀者 時(shí)間: 2025-3-24 01:38
1064-3745 end-directed cytoplasmic dynein, had been identified. At the time, these two motors seemed almost sufficient to explain directional motility events on polar microtubule tracks in the cell. No- theless, shortly after, the tip of the iceberg began to emerge with the identi- cation of proteins containi作者: cochlea 時(shí)間: 2025-3-24 05:41
Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System,e ATP-binding and the microtubule-binding domains of kinesin heavy chain, were cloned and characterized from murine brain libraries (.–.). They form kinesin superfamily proteins (KIFs). Now, 10 members (KIF1A, KIF1B, KIF2, KIF3A, KIF3B, KIF4, KIF5A, KIF5B, KIF5C, KIFC1, KIFC2, KIFC3) have been identified and characterized (.–.).作者: thalamus 時(shí)間: 2025-3-24 07:51 作者: SNEER 時(shí)間: 2025-3-24 13:20 作者: 粘 時(shí)間: 2025-3-24 18:03 作者: Latency 時(shí)間: 2025-3-24 19:21 作者: 流逝 時(shí)間: 2025-3-24 23:44 作者: 救護(hù)車 時(shí)間: 2025-3-25 03:22
Expression Cloning with Pan Kinesin Antibodies, peptide antibodies generated against two of these short, conserved sequences for the expression cloning of members of the kinesin-related protein family. We will briefly discuss the production of the pan-specific, kinesin peptide antisera and the four conserved regions within the motor domain of th作者: OPINE 時(shí)間: 2025-3-25 07:47
Green Fluorescent Protein as a Tag for Molecular Motor Proteins, methods of fluorescent labeling of proteins. Moreover, GFP fluorescence is long-lasting, and its sensitivity to pH and temperature has enabled workers to use GFP fusion proteins to measure pH in specific cellular compartments (.) and to follow protein localization in temperature-shift experiments (作者: Onerous 時(shí)間: 2025-3-25 12:40 作者: 不愿 時(shí)間: 2025-3-25 18:06 作者: 周年紀(jì)念日 時(shí)間: 2025-3-25 23:50 作者: 使出神 時(shí)間: 2025-3-26 02:45
1064-3745 fferent positions in the molecule. Their motility prop- ties are also variable in directionality, velocity, and such other characteristics as bundling activity and processivity. Finally, and mo978-1-61737-178-3978-1-59259-069-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Stricture 時(shí)間: 2025-3-26 07:39 作者: Mortar 時(shí)間: 2025-3-26 10:35
Robert L. Morris,Heather M. Brown,Brent D. Wright,David J. Sharp,William Sullivan,Jonathan M. Schole and black holes, galactic dynamics and dark-matter halos, magnetism of galaxies, interstellar gases in galaxies, and starbursts..A unique feature of this book is its focus on how to analyze and interpret radio978-981-10-3444-2978-981-10-3445-9Series ISSN 0075-8450 Series E-ISSN 1616-6361 作者: 背景 時(shí)間: 2025-3-26 15:06 作者: GRIEF 時(shí)間: 2025-3-26 18:12 作者: 地名表 時(shí)間: 2025-3-26 23:24
erstand their origin. The investigation of both detailed and global properties of the bulge now provide us with the opportunity to characterize the bulge as observed in models, and to place the mixed component bulge scenario in the general context of external galaxies. When writing this review, we c作者: 喃喃而言 時(shí)間: 2025-3-27 03:42
Sergei A. Kuznetsov,Vladimir I. Gelfandstence of an intriguing X-shaped structure in the bulge, and perhaps the metallicity gradients. We review the major theoretical models and techniques to understand the Milky Way bulge. Despite the progresses in recent theoretical attempts, a complete bulge formation model that explains the full kine作者: 一再煩擾 時(shí)間: 2025-3-27 06:35 作者: Encapsulate 時(shí)間: 2025-3-27 10:30
Laura M. Ginkel,Linda Wordemann), or stellar populations (old), are similar to those observed in the Milky Way. Cool central discs are often embedded in the B/P/barlens bulges. Barred galaxies contain also dynamically hot classical bulges, but it is not yet clear to what extent they are really dynamically distinct structure comp作者: 溫順 時(shí)間: 2025-3-27 16:33 作者: Affirm 時(shí)間: 2025-3-27 19:47 作者: HIKE 時(shí)間: 2025-3-28 01:19
Nobutaka Hirokawa,Yasuko Nodar spiral perturbations in stellar disks are described. The method is based on the analysis of periodic orbits and compares the response surface density with the imposed to estimate the degree of self-consistency. A model of the spiral NGC 5247 is used to illustrate the technique. Different examples 作者: annexation 時(shí)間: 2025-3-28 02:55 作者: parsimony 時(shí)間: 2025-3-28 09:29
Erik Nielsen,Fedor Severin,Anthony A. Hyman,Marino Zerialldwin, D. S. Heeschen, F. J. Kerr, J. Lequeux, S. W. McCuskey, P. G. Mezger, B. Y. Mills, Yu. N. Parijskij, B. J. Robinson, H. van der Laan, and H. F. Weaver. The Local Committee, consisting ofB. J. Robinson, N. G. Seddon, and P. J. Kelly, looked after the arrangements in very fine style. The Sympos作者: ASSAY 時(shí)間: 2025-3-28 11:38 作者: 聲音刺耳 時(shí)間: 2025-3-28 18:01
Vladimir I. Gelfand,Nathalie Le Bot,M. Carolina Tuma,Isabelle Vernos medium, which contains both thermal electrons and magnetic fields, can be decomposed into two oppositely circularly polarized components having different phase velocities. On emergence from the medium the waves can be recombined, but with relative phases which may be different from those which they作者: humectant 時(shí)間: 2025-3-28 22:27 作者: 好色 時(shí)間: 2025-3-28 23:55 作者: Bronchial-Tubes 時(shí)間: 2025-3-29 07:03
Manfred Thorm?hlen,Jens Müller,Eckhard Mandelkowstressed, rather than those expected from more homogeneous components such as silicates, graphite, or amorphous carbon since these have been discussed in considerable detail elsewhere (e.g. Kr?tschmer and Huffman, 1979, Knacke and Kr?tschmer, 1980, Day, 1979, Borghesi et al., 1983 and references the作者: Recess 時(shí)間: 2025-3-29 07:15
RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superf including over 30 in the mouse alone (.). The common feature of the kinesin-like proteins is a highly conserved 350-amino-acid “motor domain” (30–40% sequence identity across species) that includes even more highly conserved ATP- and microtubule-binding sites (.).作者: Angiogenesis 時(shí)間: 2025-3-29 12:48
Expression Cloning with Pan Kinesin Antibodies,orm force-generating movements along filamentous microtubules. Members of this family of microtubule motors are vital for numerous cellular activities, such as organelle transport and chromosome segregation. Kinesin-related proteins are not only linked by function, but each family member shares a si作者: 新奇 時(shí)間: 2025-3-29 18:46 作者: arterioles 時(shí)間: 2025-3-29 22:33 作者: capsaicin 時(shí)間: 2025-3-30 03:57
An Improved Microscope for Bead and Surface-Based Motility Assays,ncoated surfaces or the motility of kinesin-coated beads on immobilized single microtubules. A Zeiss Axiovert was modified by replacing the standard Zeiss condenser and mechanical stage with an improved arrangement that provides much better mechanical stability and very high resolution differential 作者: 性學(xué)院 時(shí)間: 2025-3-30 04:32 作者: 松果 時(shí)間: 2025-3-30 09:07 作者: IRK 時(shí)間: 2025-3-30 14:31
Green Fluorescent Protein as a Tag for Molecular Motor Proteins,r in living cells when fused to another protein (.), GFP has come into wide use. GFP offers advantages over other reporters in that it fluoresces in live cells and its fluorescence does not require the addition of any substrates, cofactors, or accessory proteins. This means that GFP-tagged proteins 作者: Dri727 時(shí)間: 2025-3-30 20:00 作者: 拖網(wǎng) 時(shí)間: 2025-3-30 21:07 作者: 依法逮捕 時(shí)間: 2025-3-31 04:19 作者: 能得到 時(shí)間: 2025-3-31 07:29
The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindlghly dynamic bipolar structure assembles and disassembles periodically and its main structural components are microtubules (MTs) and chromosomes. Microtubule-dependent motors in particular members of the kinesin superfamily play an important role in directing the self-organization of microtubules in作者: Flatus 時(shí)間: 2025-3-31 13:11 作者: 協(xié)議 時(shí)間: 2025-3-31 14:52 作者: Reclaim 時(shí)間: 2025-3-31 20:31
Assaying Spatial Organization of Microtubules by Kinesin Motors,state, which makes them able to simultaneously bind two microtubules. Here, we describe an in vitro assay for the direct observation of the organization of microtubules by these motors. With this assay, patterns of microtubules are formed that reflect the morphogenetic abilities of the motors. The a作者: 不可接觸 時(shí)間: 2025-4-1 01:02
Crystallization of Kinesin,ventional kinesin consists of two identical heavy chains carrying the motor activity, and two light chains. Based on its amino acid sequence, the heavy chain can be divided into three separate domains: (.) an N-terminal head, or motor domain, (.) an elongated a-helical stretch forming a coiled coil 作者: Friction 時(shí)間: 2025-4-1 03:42 作者: HAIL 時(shí)間: 2025-4-1 07:50
Sergei A. Kuznetsov,Vladimir I. Gelfand witnessed a gradual paradigm shift in the formation and evolution of the Galactic bulge. Bulges were commonly believed to form in the dynamical violence of galaxy mergers. However, it has become increasingly clear that the main body of the Milky Way bulge is not a classical bulge made by previous m作者: G-spot 時(shí)間: 2025-4-1 10:55 作者: Jargon 時(shí)間: 2025-4-1 14:51
Laura M. Ginkel,Linda Wordeman edge-on galaxies have these characteristics, which fraction is similar to the observed bar fraction in Hubble types earlier than Scd. B/P bulges are generally detected in the edge-on view, but it has been recently demonstrated that barlenses, which are lens-like structures embedded in bars, are the作者: Inflated 時(shí)間: 2025-4-1 18:39
Maryanne F. Stock,David D. Hackney witnessed a gradual paradigm shift in the formation and evolution of the Galactic bulge. Bulges were commonly believed to form in the dynamical violence of galaxy mergers. However, it has become increasingly clear that the main body of the Milky Way bulge is not a classical bulge made by previous m作者: rheumatology 時(shí)間: 2025-4-1 22:59 作者: 情感脆弱 時(shí)間: 2025-4-2 05:42
Nobutaka Hirokawa,Yasuko Nodals of spiral galaxies and the comparison with observations yield important information on the basic state of such systems which otherwise cannot be obtained. Normal, grand design spirals are emphasized because they show many of the basic dynamic effects more clearly. The morphology of spiral galaxie作者: syncope 時(shí)間: 2025-4-2 09:57
