作者: 一再煩擾 時(shí)間: 2025-3-21 21:34
Ames C. Register,Sujata Chakraborty,Dustin J. Maly作者: Admonish 時(shí)間: 2025-3-22 02:00 作者: 安慰 時(shí)間: 2025-3-22 06:13 作者: 咆哮 時(shí)間: 2025-3-22 09:10 作者: allergy 時(shí)間: 2025-3-22 13:14
Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quan-based xMAP. platform from Luminex. The system utilizes antibody-bound free-floating magnetic spheres which can easily be removed from suspension via magnetization. There are 100 unique bead sets (moving up to 500 bead sets for the most recent system) identified by the ratio of two dyes coating the 作者: incredulity 時(shí)間: 2025-3-22 17:11
High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticitywing cell fixation while also preserving the fluorescence of a stably expressed fluorescent protein-tagged reporter for CDK2 activity. We provide methodology for data extraction and handling including an R-script that converts the multidimensional data into four simple binary outcomes, on which a hi作者: Anhydrous 時(shí)間: 2025-3-22 21:51 作者: capillaries 時(shí)間: 2025-3-23 01:42 作者: 切割 時(shí)間: 2025-3-23 06:53 作者: 冒失 時(shí)間: 2025-3-23 10:38
1064-3745 iding known pitfalls.?.Authoritative and practical, .Kinase Signaling Networks. aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology..978-1-4939-8400-8978-1-4939-7154-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: antiandrogen 時(shí)間: 2025-3-23 15:53
Anne-Marie Ray,Jennifer E. Klomp,Kerrie B. Collins,Andrei V. Karginovis creating a new set of data management requirements involving XML. Currently, databases and XML play important roles for data management and have become the main means to realize the data management. In this chapter, databases and XML techniques for data management will be introduced.作者: 微枝末節(jié) 時(shí)間: 2025-3-23 18:38
Michael Pargett,Taryn E. Gillies,Carolyn K. Teragawa,Breanne Sparta,John G. Albeckis creating a new set of data management requirements involving XML. Currently, databases and XML play important roles for data management and have become the main means to realize the data management. In this chapter, databases and XML techniques for data management will be introduced.作者: 虛構(gòu)的東西 時(shí)間: 2025-3-23 22:27 作者: 呼吸 時(shí)間: 2025-3-24 06:07 作者: Infuriate 時(shí)間: 2025-3-24 07:27
Martin Golkowski,Dustin J. Maly,Shao-En Onge to call them cooperative games with multiple scenarios or cooperative games under uncertainty. Bergstresser and Yu mainly considered the core defined by the domination structures and referred to a couple of solution concepts which yield a unique solution such as the nucleolus in .-person cooperati作者: 辮子帶來幫助 時(shí)間: 2025-3-24 13:41 作者: jungle 時(shí)間: 2025-3-24 18:31 作者: grudging 時(shí)間: 2025-3-24 21:12 作者: 兵團(tuán) 時(shí)間: 2025-3-25 02:22
Kinase Signaling Networks978-1-4939-7154-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Gnrh670 時(shí)間: 2025-3-25 03:40
https://doi.org/10.1007/978-1-4939-7154-1Protein science; Synthetic biology; Screening approaches; Mass spectrometry; Computational analysis; Chem作者: Carbon-Monoxide 時(shí)間: 2025-3-25 11:04
Aik-Choon Tan,Paul H. HuangIncludes cutting-edge techniques for the study of kinase signaling networks.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts.Includes作者: 颶風(fēng) 時(shí)間: 2025-3-25 12:35 作者: Ergots 時(shí)間: 2025-3-25 19:13
Book 2017ep-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Kinase Signaling Networks. aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology..作者: 殘酷的地方 時(shí)間: 2025-3-25 23:33 作者: 特別容易碎 時(shí)間: 2025-3-26 03:32 作者: 拱墻 時(shí)間: 2025-3-26 06:49 作者: Juvenile 時(shí)間: 2025-3-26 11:38 作者: Hormones 時(shí)間: 2025-3-26 15:28 作者: 錯(cuò)事 時(shí)間: 2025-3-26 20:07
Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensorsneeded to perform such measurements in a cell type of the investigator’s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.作者: Cardioplegia 時(shí)間: 2025-3-26 23:20 作者: 蜿蜒而流 時(shí)間: 2025-3-27 02:50 作者: poliosis 時(shí)間: 2025-3-27 07:35
Dissecting Kinase Effector Signaling Using the RapRTAP Methodologyplex in living cells. This technology uses a rapamycin-inducible kinase activation coupled to interaction with FKBP12-binding domain (FRB) tagged protein. Here, we demonstrate application of this method by targeting Src to either p130Cas or FAK and discriminating cell mophodynamic changes downstream each of these signaling complexes.作者: UNT 時(shí)間: 2025-3-27 11:20
Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometryan example, we provide an optimized protocol featuring on-bead protein digestion and single nano-flow liquid chromatographic-mass spectrometric (LC-MS) analyses, consequently increasing analytical throughput and sensitivity over gel-based sample preparation methods for rapid profiling of kinase inhibitor targets.作者: 食品室 時(shí)間: 2025-3-27 14:56
Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Linesust two-stage isolation of phosphotyrosine peptides and mass spectrometry-aided identification of phosphosites to characterize basal signaling networks in unstimulated non-small cell lung cancer (NSCLC) cell lines.作者: 悲觀 時(shí)間: 2025-3-27 20:25 作者: Calculus 時(shí)間: 2025-3-27 23:44 作者: GLIDE 時(shí)間: 2025-3-28 04:39
Dissecting Kinase Effector Signaling Using the RapRTAP Methodologyponse. Dissecting downstream signaling pathways of kinases is a key step to understanding physiological and pathological cell process. However, directing kinase activity to specific substrates remains challenging. Here, we present a new tool to selectively activate a kinase in a specific protein com作者: 顯微鏡 時(shí)間: 2025-3-28 09:44 作者: daredevil 時(shí)間: 2025-3-28 13:50
Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors disease phenotypes. When activity data is collected across a panel of kinases, a unique “signaling fingerprint” is generated that allows for discrimination between diseased and normal tissue. Here we describe the use of peptide-based kinase activity sensors to fingerprint the signaling changes asso作者: debunk 時(shí)間: 2025-3-28 18:05
Expression of Recombinant Phosphoproteins for Signal Transduction Studiesf recombinant phosphoproteins by directly incorporating phosphoserine as a nonstandard amino acid. This protocol utilizes an optimized phosphoserine orthogonal translation system and an engineered strain of . containing no genomic amber codons. This approach has been used to generate a variety of ph作者: 暫時(shí)過來 時(shí)間: 2025-3-28 22:14
Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitorsf SFK SH2 and SH3 domains with their intramolecular ligands leads to reduced kinase activity by stabilizing an inactive ATP-binding site conformation. Disruption of these intramolecular interactions stabilizes a more active ATP-binding site conformation and restores SFK activity. Interestingly, this作者: JOG 時(shí)間: 2025-3-28 23:07 作者: notion 時(shí)間: 2025-3-29 05:34
Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometryrapeutics. Our rapid small-molecule target profiling protocol combines affinity enrichment and SILAC for proteomic identification of small molecule-protein interactions. Selective interactions are easily discernable from nonspecific protein binding by quantitative ratios. Using kinase inhibitors as 作者: 大約冬季 時(shí)間: 2025-3-29 11:17
Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quanphorylation, the addition of phosphate groups, most often occurs on serine, threonine, or tyrosine residues due to the action of protein kinases. This structural change causes the protein to become activated (or deactivated) and enables it in turn to initiate the phosphorylation of other proteins in作者: 古老 時(shí)間: 2025-3-29 14:47 作者: 進(jìn)取心 時(shí)間: 2025-3-29 15:43 作者: deforestation 時(shí)間: 2025-3-29 20:34 作者: Palate 時(shí)間: 2025-3-30 03:21
Label-Free Phosphoproteomic Approach for Kinase Signaling Analysismed by means of either labeling or label-free mass spectrometry (MS) methods. Because of their simplicity and universality, label-free methodology is gaining acceptance and popularity in molecular biology research. Analytical workflows for label-free quantification of phosphorylation, however, need 作者: 群居男女 時(shí)間: 2025-3-30 05:28
Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometryue insight in contact-initiated receptor tyrosine kinase signaling, transfer of proteomic material between heterotypic cells, and interactions between normal and oncogenic cells. Here we describe current methods for cell-specific labeling of heterotypic cells with isotopic labeled amino acids (e.g.,作者: 有說服力 時(shí)間: 2025-3-30 09:29
Characterization of the Phospho-Adhesome by Mass Spectrometry-Based Proteomics fundamental cell functions. Protein kinases and phosphatases play an integral role in integrin adhesion-mediated signaling. However, until recently, knowledge of the phosphorylation sites regulated downstream of integrin ligation was limited to candidate-based approaches and did not support a syste作者: CUMB 時(shí)間: 2025-3-30 14:55
Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Linesrned by protein tyrosine kinases, including kinases involved in oncogenesis. Phosphotyrosine (pY)-containing peptides are commonly isolated from cellular lysates by means of antibody and/or metal affinity-based enrichment followed by their identification by mass spectrometry. Herein, we describe rob作者: Recess 時(shí)間: 2025-3-30 17:27
Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometryrrant signaling driving cancer progression. However, validating the behavior of specific phosphosites across multiple experimental conditions remains challenging, due to limitations inherent in discovery-based proteomic workflows and the limited availability of high-quality antibodies required for a作者: obnoxious 時(shí)間: 2025-3-30 21:35 作者: 雪白 時(shí)間: 2025-3-31 01:30
Michael Pargett,Taryn E. Gillies,Carolyn K. Teragawa,Breanne Sparta,John G. Albeckto operate large quantities of data by inputting, storing, retrieving, and managing that data. Over the years, various database models, including conceptual data models (e.g., entity-relationship (ER) model, enhanced entity-relationship (EER) model, and UML data model) and logical database models (e作者: macrophage 時(shí)間: 2025-3-31 06:45
Jon R. Beck,Edward N. Harris,Cliff I. Stainsful direction. Further developments are needed to consider other aspects and thus to make the actual solution process more practical in the sense of solving more realistic problems with reduced computation. Two of the most important aspects which need to be considered are the problem of aggregation 作者: 瘙癢 時(shí)間: 2025-3-31 13:05 作者: 檢查 時(shí)間: 2025-3-31 15:08
Martin Golkowski,Dustin J. Maly,Shao-En Ong- person cooperative games under fuzziness, uncertainty or risk. To introduce fuzziness, uncertainty or risk into a cooperative game, we define a mapping which associates a coalition with a fuzzy set or a probability distribution, instead of the characteristic function. A representation of cooperati作者: 可用 時(shí)間: 2025-3-31 18:48
Adam Stewart,Udai Banerji- person cooperative games under fuzziness, uncertainty or risk. To introduce fuzziness, uncertainty or risk into a cooperative game, we define a mapping which associates a coalition with a fuzzy set or a probability distribution, instead of the characteristic function. A representation of cooperati作者: 小平面 時(shí)間: 2025-3-31 22:41
Joseph Robertson,Jonathan D. Humphries,Nikki R. Paul,Stacey Warwood,David Knight,Adam Byron,Martin Jptides. Firstly, 489 informative features are extracted based on 11 interesting compositions. Thereafter, 117 non-redundant and relevant features are selected by using fuzzy rough feature selection (FRFS) with ant colony optimization (ACO) search. Then, instances of the reduced dataset are resampled作者: Amnesty 時(shí)間: 2025-4-1 01:49
9樓作者: 節(jié)約 時(shí)間: 2025-4-1 07:52
10樓作者: FLASK 時(shí)間: 2025-4-1 10:37
10樓作者: EXULT 時(shí)間: 2025-4-1 16:06
10樓作者: insidious 時(shí)間: 2025-4-1 18:49
10樓
