標(biāo)題: Titlebook: KRAS; Methods and Protocol Andrew G. Stephen,Dominic Esposito Book 2024 Leios Biomedical Research, Inc. 2024 GTPase activity.mass spectrome [打印本頁] 作者: Pessimistic 時間: 2025-3-21 18:48
書目名稱KRAS影響因子(影響力)
書目名稱KRAS影響因子(影響力)學(xué)科排名
書目名稱KRAS網(wǎng)絡(luò)公開度
書目名稱KRAS網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱KRAS被引頻次
書目名稱KRAS被引頻次學(xué)科排名
書目名稱KRAS年度引用
書目名稱KRAS年度引用學(xué)科排名
書目名稱KRAS讀者反饋
書目名稱KRAS讀者反饋學(xué)科排名
作者: excursion 時間: 2025-3-21 21:00 作者: 擁護(hù) 時間: 2025-3-22 02:35
Book 2024 novel molecules that bind KRAS in live cells. Chapters focus on protein production and characterization, biochemical assays, cell-based assays, KRAS-membrane interactions, targeting KRAS, and cell models. Written in the highly successful?.Methods in Molecular Biology.?series format, chapters includ作者: 向外 時間: 2025-3-22 05:45
Book 2024d key tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.KRAS: Methods and Protocols .aims to provide methods that will be instrumental in the development of future clinically approved KRAS therapeutics..作者: 革新 時間: 2025-3-22 09:28 作者: 草率女 時間: 2025-3-22 16:05
Studying RAS Interactions in Live Cells with BRET,RAF1 binding to active RAS, exhibits nonspecific interactions. The assay exhibits remarkable signal-to-background ratios and is highly suitable for investigating the interactions of RAS with effectors, as well as for high-throughput screening assays.作者: 離開 時間: 2025-3-22 18:03 作者: 討人喜歡 時間: 2025-3-22 23:33
Considerations Around Structure-Based Drug Discovery for KRAS Using DOCK,for prosecuting a virtual screen: protein structure choice, pocket selection, optimization of the scoring function, modification of sampling spheres and sampling procedures, choosing an appropriate portion of chemical space to dock, and the choice of which top scoring molecules to pick for purchase.作者: Geyser 時間: 2025-3-23 04:40
The Abundance of KRAS and RAS Gene Mutations in Cancer,imate . mutations occur in 11–14% of all human cancers. Although these estimates are lower than many commonly encountered values, these estimates continue to rank . and the ensemble of . oncogenes among the most common genetic drivers of cancer across all forms of malignancy.作者: 哥哥噴涌而出 時間: 2025-3-23 09:33
Measurement of KRAS-GTPase Activity,olysis. Here we describe a biochemical assay that measures the release of free phosphate upon hydrolysis of the GTP nucleotide and allows the measurement of intrinsic or GAP-stimulated GTP hydrolysis by KRAS. This assay can be used to measure GTPase activity under single turnover conditions.作者: 善于 時間: 2025-3-23 10:59
Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 tof molecular interactions but also association and dissociation rates of such interactions. In this chapter, we describe a general method to measure the affinity of a small molecule drug, MRTX849, to GDP bound HRAS, KRAS, and NRAS.作者: 小鹿 時間: 2025-3-23 14:43 作者: perjury 時間: 2025-3-23 21:57 作者: Slit-Lamp 時間: 2025-3-24 01:54 作者: Nausea 時間: 2025-3-24 03:25
KRAS4b:RAF-1 Homogenous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Drug Discovees compared to traditional assay techniques. In this chapter, we describe an HTRF protein-protein interaction (PPI) assay for the KRAS4b G-domain in the GppNHp-bound state and the RAF-1-RBD currently used for drug screens. Application of this assay contributes to the identification of lead compounds targeting the GTP-bound active state of K-RAS.作者: Hiatal-Hernia 時間: 2025-3-24 08:11 作者: Amplify 時間: 2025-3-24 14:33
978-1-0716-3824-8Leios Biomedical Research, Inc. 2024作者: MIME 時間: 2025-3-24 18:33
KRAS978-1-0716-3822-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 模范 時間: 2025-3-24 20:08
Andrew G. Stephen,Dominic EspositoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 有斑點(diǎn) 時間: 2025-3-25 01:23 作者: 提名的名單 時間: 2025-3-25 07:18
https://doi.org/10.1007/978-1-0716-3822-4GTPase activity; mass spectrometry; post-translational modifications; KRAS membrane diffusion; lipid bil作者: 相一致 時間: 2025-3-25 08:38 作者: 制定 時間: 2025-3-25 12:52 作者: 牙齒 時間: 2025-3-25 17:43 作者: 泥沼 時間: 2025-3-25 20:20
Edward C. Stitesipschitz continuous functions on [0,1] such that . For simplicity, we assume the coefficients ..=1, ?.≠.. Without this assumption, our proof is still valid with minor changes, due to the special property of dimension?1. In the following, we will point out this whenever necessary. From the discussion作者: PRISE 時間: 2025-3-26 01:43
Troy Taylor,William Gillettecs have been obtained for the following problems: wave diffraction by a bottom obstacle, evolution of an initial disturbance for one-and two-layer fluids, for gravity - capillary waves and for fluid layer on an elastic base. All the mentioned asymptotics take into account the focusing effects. Their作者: Ceremony 時間: 2025-3-26 04:52 作者: 精密 時間: 2025-3-26 08:37
Albert H. Chan,Dhirendra K. Simanshu?.When the system is open, there are several mechanisms to couple the system with the external forces. Here a class of systems where the interaction with the exterior takes place in correspondence of a free boundary is considered. Both continuous and discrete models sharing the same structure are an作者: 易于出錯 時間: 2025-3-26 15:16
Mayukh Chakrabarti,Y. Stanley Tan,Trent E. Baliusc laws of transport are described by PDE‘s.?.When the system is open, there are several mechanisms to couple the system with the external forces. Here a class of systems where the interaction with the exterior takes place in correspondence of a free boundary is considered. Both continuous and discre作者: 休戰(zhàn) 時間: 2025-3-26 20:34 作者: seruting 時間: 2025-3-26 21:31 作者: ATOPY 時間: 2025-3-27 04:20 作者: JIBE 時間: 2025-3-27 06:49
Kari Koprace. For the two-phase Stefan problem, for instance, the order of convergence in .. for piecewise linear approximations of temperature is never better than linear [13,14], even though one might expect quadratic convergence according to the interpolation theory. Theoretical results are even more pessi作者: Calculus 時間: 2025-3-27 10:44
Marcin Dyba,John-Paul Denson,Anna E. Maciagh a Lipschitz boundary Γ, covered by a finite number of disjoint polyhedral subdomains Ω., .=1,...,.; that means Ω.?Ω, Ω.∩Ω.=? for .., . , the bar denotes the closure. The nonlinear heat-transfer equation will have in each subdomain Ω. its own (temperature-dependent) coefficients of heat capacity ..作者: PAD416 時間: 2025-3-27 13:40
Erik K. Larsen,Maria Abreu-Blanco,Dana Rabara,Andrew G. Stephenm boiling and condensation of Newtonian fluids, as well as accelerating film flow of non-Newtonian power-law fluids (FFNF). A novel system of analysis models is provided with a developed velocity component method, instead of traditional Falkner-Skan type transformation, and a system of models for tr作者: BALK 時間: 2025-3-27 18:10
Sangho Yun,Elena Scott,Arthur Laganowsky systematic studies of hydrodynamics and heat and mass transfer in laminar free convection, accelerating film boiling and condensation of Newtonian fluids, as well as accelerating film flow of non-Newtonian power-law fluids (FFNF). A novel system of analysis models is provided with a developed veloc作者: DAUNT 時間: 2025-3-28 01:36 作者: 溫室 時間: 2025-3-28 03:53 作者: 注意到 時間: 2025-3-28 08:44 作者: incredulity 時間: 2025-3-28 13:27
Qingci Zhao,Ichio Shimada,Noritaka Nishida systematic studies of hydrodynamics and heat and mass transfer in laminar free convection, accelerating film boiling and condensation of Newtonian fluids, as well as accelerating film flow of non-Newtonian power-law fluids (FFNF). A novel system of analysis models is provided with a developed veloc作者: triptans 時間: 2025-3-28 15:07 作者: 爆炸 時間: 2025-3-28 20:20 作者: 爆炸 時間: 2025-3-28 23:34
Progress in Targeting KRAS Directly,different types of cancer and the effects of these mutations on the biochemical, structural, and biophysical properties of the RAS proteins themselves, particularly KRAS, on which most attention has been focused. This knowledge base, while still growing, has enabled creative chemical approaches to t作者: Tortuous 時間: 2025-3-29 03:24 作者: 爭論 時間: 2025-3-29 08:07 作者: 過份 時間: 2025-3-29 11:23
Nucleotide Exchange on RAS Proteins Using Hydrolysable and Non-hydrolysable Nucleotides, exchange the nucleotide present in the purified RAS protein with either GDPβS, GppNHp, or GTP depending on the assay requirement. In addition, we also describe the HPLC method used to validate the exchange process and provide information on the efficiency of the nucleotide exchange.作者: 堅(jiān)毅 時間: 2025-3-29 17:59 作者: ascend 時間: 2025-3-29 22:33
Considerations Around Structure-Based Drug Discovery for KRAS Using DOCK,ll molecules to cavities on the surfaces of proteins. Virtual screening for ligand discovery is a useful application of docking software. In this chapter, using the enigmatic KRAS protein as an example system, we endeavor to teach the reader about best practices for performing molecular docking with作者: 嬉耍 時間: 2025-3-30 02:00
Measurement of KRAS-GTPase Activity, The development of biochemical assays for GTPase activity provides an opportunity to quantitatively measure the impact of these mutations on GTP hydrolysis. Here we describe a biochemical assay that measures the release of free phosphate upon hydrolysis of the GTP nucleotide and allows the measurem作者: constellation 時間: 2025-3-30 06:27
Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 ts label free and versatile, not limited to proteins, nucleic acids, and small molecules. SPR is a widely accepted method to measure not only affinity of molecular interactions but also association and dissociation rates of such interactions. In this chapter, we describe a general method to measure t作者: COW 時間: 2025-3-30 11:32 作者: 占卜者 時間: 2025-3-30 12:27
Luminescence-Based Techniques for KRAS Thermal Stability Monitoring,uminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced作者: 攀登 時間: 2025-3-30 20:25 作者: 文件夾 時間: 2025-3-30 22:23 作者: Malaise 時間: 2025-3-31 03:27 作者: Filibuster 時間: 2025-3-31 05:10 作者: 煞費(fèi)苦心 時間: 2025-3-31 10:25 作者: Vulnerable 時間: 2025-3-31 15:12
Analysis of the Guanine Nucleotide-Bound State of KRAS by Ion-Pair Reversed-Phase High-Performance (IP-RP-HPLC)-based method, which enables analyzing GDP and GTP bound to small GTPases immunoprecipitated from cells. The activation status of FLAG-KRAS expressed in HEK293T cells can be investigated with the IP-RP-HPLC method. This method also can be adapted to determine the effects of compounds suc作者: 信徒 時間: 2025-3-31 19:46 作者: 小說 時間: 2025-4-1 01:12 作者: GRAIN 時間: 2025-4-1 02:53 作者: 結(jié)果 時間: 2025-4-1 06:22
