作者: Middle-Ear 時(shí)間: 2025-3-21 21:53
Exploring Protein Intrinsic Disorder with MobiDBrder predictions from protein sequences. It provides an easy-to-use web server to visualize and explore disorder information. This chapter describes the data available in MobiDB, emphasizing how to use and access the intrinsic disorder data. MobiDB is available at URL ..作者: 盡忠 時(shí)間: 2025-3-22 01:50 作者: Statins 時(shí)間: 2025-3-22 05:30 作者: Emg827 時(shí)間: 2025-3-22 11:55 作者: 安慰 時(shí)間: 2025-3-22 12:55
Computational Prediction of Disordered Protein Motifs Using SLiMSuite and online server examples are provided. Detailed notes provide additional advice on different applications of SLiMSuite, including batch running of multiple datasets and conservation masking using alignments of predicted orthologues.作者: JOG 時(shí)間: 2025-3-22 20:46 作者: colostrum 時(shí)間: 2025-3-22 22:09
Expression and Purification of an Intrinsically Disordered Protein hexahistidine sequence (His-tag). This vector allows for the SUMO-AtLEA 3-2 fusion protein to be purified using Ni-affinity chromatography and, through the use of ubiquitin-like-specific protease 1 (Ulp1, a SUMO protease), results in an AtLEA 3-2 with a native N-terminus. We also describe the expression and purification of Ulp1 itself.作者: 斷言 時(shí)間: 2025-3-23 02:12 作者: 舉止粗野的人 時(shí)間: 2025-3-23 07:11
Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopyabelling of the produced protein is defined by the chosen amino acids during reaction setup. Here we describe how to set up production and isotopic labelling of small intrinsically disordered proteins (IDPs) for NMR spectroscopy applications using an .-based CFPS system in batch mode.作者: breadth 時(shí)間: 2025-3-23 10:43 作者: CRAMP 時(shí)間: 2025-3-23 16:09
Determining , of IDPs from SAXS DataFranke et al., J Appl Crystallogr 50:1212–1225, 2017) has been used for the evaluation, and throughout the chapter, different examples will be given to illustrate the discussed phenomena, as well as the pros and cons of using the different approaches.作者: 針葉類(lèi)的樹(shù) 時(shí)間: 2025-3-23 20:39 作者: myalgia 時(shí)間: 2025-3-24 00:10 作者: 刪減 時(shí)間: 2025-3-24 04:14
https://doi.org/10.1007/978-1-0716-0524-0E; coli expression; post-translational modification; NMR; SAXS data; mass spectrometry作者: 凌辱 時(shí)間: 2025-3-24 07:55 作者: LASH 時(shí)間: 2025-3-24 12:48 作者: chronicle 時(shí)間: 2025-3-24 17:51
Birthe B. Kragelund,Karen SkriverIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Perigee 時(shí)間: 2025-3-24 19:17 作者: PRE 時(shí)間: 2025-3-25 00:31
Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tract information. Here, we present a protocol to prepare monomeric samples of isotope-enriched short helical polyQ peptides based on the sequence of the androgen receptor (AR) suitable for NMR characterization and suggest different ways to adapt it for the production and monomerization of other relative作者: fleeting 時(shí)間: 2025-3-25 06:04 作者: minaret 時(shí)間: 2025-3-25 10:22
Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient sed to, with good precision, monitor changes in the ensemble size properties upon functional interactions of the disordered protein, e.g., dimerization, ligand binding, and folding pathways. Here, we present a step-by-step protocol for diffusion measurements using pulsed field gradient nuclear magne作者: 憤慨點(diǎn)吧 時(shí)間: 2025-3-25 14:51
1064-3745 ringer.com..Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..978-1-0716-0526-4978-1-0716-0524-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: faction 時(shí)間: 2025-3-25 19:42 作者: corpus-callosum 時(shí)間: 2025-3-25 22:07
nanderen B?nden. Diese erfassen das Fachwissen für die zunehmend wichtigen Gebiete der elektrischen Antriebstechnik an sich und angrenzender Gebiete mit Komponenten der elektrischen Antriebstechnik, wie z.B. Ne978-3-642-30471-2Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 刺穿 時(shí)間: 2025-3-26 01:43
Janelle Nunez-Castilla,Jessica Siltberg-Liberleslehrt wird. Es wurde gr??te Aufmerksamkeit auf hohe Verst?ndlichkeit gesetzt. Und so ist das Werk gleicherma?en in Universit?ten und Fachhochschulen und als Nachschlagewerk in der Praxis einsetzbar. Den Grundla978-3-642-02990-5Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 刻苦讀書(shū) 時(shí)間: 2025-3-26 07:55
Christopher J. Oldfield,Xiao Fan,Chen Wang,A. Keith Dunker,Lukasz Kurgan作者: 規(guī)范就好 時(shí)間: 2025-3-26 12:15
Richard J. Edwards,Kirsti Paulsen,Carla M. Aguilar Gomez,?sa Pérez-Bercoff作者: 的事物 時(shí)間: 2025-3-26 15:48
Marc Gouw,Jesús Alvarado-Valverde,Jelena ?aly?eva,Francesca Diella,Manjeet Kumar,Sushama Michael,Kim作者: evanescent 時(shí)間: 2025-3-26 18:14 作者: 高腳酒杯 時(shí)間: 2025-3-27 00:23
Alexander Miguel Monzon,András Hatos,Marco Necci,Damiano Piovesan,Silvio C. E. Tosatto zu den benachbarten Gebieten sowie ein Stichwortverzeichnis der behandelten Gebiete in denanderen B?nden. Diese erfassen das Fachwissen für die zunehmend wichtigen Gebiete der elektrischen Antriebstechnik an sich und angrenzender Gebiete mit Komponenten der elektrischen Antriebstechnik, wie z.B. Ne作者: Lumbar-Spine 時(shí)間: 2025-3-27 04:29
ktrischen Energietechnik, der Automatisierungstechnik oder verwandter Studienrichtungen gelehrt wird. Es wurde gr??te Aufmerksamkeit auf hohe Verst?ndlichkeit gesetzt. Und so ist das Werk gleicherma?en in Universit?ten und Fachhochschulen und als Nachschlagewerk in der Praxis einsetzbar. Den Grundla作者: laxative 時(shí)間: 2025-3-27 08:14
Karamjeet K. Singh,Steffen P. Graether werden, da? oberhalb der Grenzkreisfrequenz der Führungsfrequenzgang mit mindestens 20 . abf?llt, um St?rsignale zu d?mpfen. Wenn somit das Führungsverhalten festgelegt ist, dann kann aufgrund der Kenntnis der Strecke und des gewünschten Frequenzverhaltens der Regler entwickelt werden [7, 9, 12, 50作者: BUOY 時(shí)間: 2025-3-27 11:21
Jakob T. Nielsen,Frans A. A. Mulderl und in einem weiten Bereich ?ndert (Lichtbogenofen in der Einschmelzphase, in diesem Fall wird der frisch eingefüllte Schrott eingeschmolzen). Das führt zu Lichtb?gen, die jeweils zur n?chsten Schrottspitze springen; dadurch bedingt ?ndert sich die L?nge des Lichtbogens und somit die Leistungschar作者: 共同時(shí)代 時(shí)間: 2025-3-27 15:28
Book 2020s into these fascinating and dynamic molecules..Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com..Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..作者: cylinder 時(shí)間: 2025-3-27 19:41
Disorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteinssites for posttranslational modifications and protein–protein interactions into arrays of short linear motifs that can be reorganized by RNA splicing. The evolution of disordered regions looks different from their ordered counterparts. In some cases, selection is focused on maintaining protein bindi作者: 傲慢人 時(shí)間: 2025-3-28 01:25 作者: negotiable 時(shí)間: 2025-3-28 05:04
Computational Prediction of Disordered Protein Motifs Using SLiMSuites. Here, we detail instructions for the computational prediction of SLiMs in disordered protein regions, using the main tools of the SLiMSuite package: (1) SLiMProb identifies and calculates enrichment of predefined motifs in a set of proteins; (2) SLiMFinder predicts SLiMs de novo in a set of prote作者: heterogeneous 時(shí)間: 2025-3-28 08:34
How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resourcese disordered regions are rich in short linear motifs (SLiMs) which mediate protein–protein interactions. Although these motifs are short and often partially conserved, they are involved in many important aspects of protein function, including cleavage, targeting, degradation, docking, phosphorylati作者: OVERT 時(shí)間: 2025-3-28 14:06 作者: custody 時(shí)間: 2025-3-28 17:57
Exploring Protein Intrinsic Disorder with MobiDBically disordered. MobiDB is the main repository of protein disorder and mobility annotations, combining different data sources to provide an exhaustive overview of intrinsic disorder. MobiDB includes curated annotations from other databases, indirect disorder evidence from structural data, and diso作者: 懦夫 時(shí)間: 2025-3-28 22:12 作者: Intellectual 時(shí)間: 2025-3-29 01:12 作者: Schlemms-Canal 時(shí)間: 2025-3-29 05:50
Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricksconsiderable effort has been put into the structural characterization of their conformational ensembles, to understand the link between the transient, short- and long-range organizations of IDPs and their functions. Such biophysical studies require substantial amounts of pure protein, representing a作者: 浪費(fèi)物質(zhì) 時(shí)間: 2025-3-29 09:34
Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tractome low-complexity regions (LCRs) and short linear motifs (SLiMs). In particular, polyglutamine (polyQ) tracts are LCRs of variable length highly enriched in glutamine residues. They are common in transcription factors, and their length can have an impact on transcriptional activity. In nine protein作者: Juvenile 時(shí)間: 2025-3-29 12:10
Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopyse of CFPS has almost from the onset been favorably linked to the production of isotopically labelled proteins for NMR spectroscopy as the resulting labelling of the produced protein is defined by the chosen amino acids during reaction setup. Here we describe how to set up production and isotopic la作者: 1分開(kāi) 時(shí)間: 2025-3-29 16:36 作者: FLOAT 時(shí)間: 2025-3-29 22:26 作者: insipid 時(shí)間: 2025-3-30 01:47 作者: BARB 時(shí)間: 2025-3-30 04:41
Quantitative Protein Disorder Assessment Using NMR Chemical Shifts and pluripotency of intrinsically disordered proteins (IDPs). The first step toward a better understanding of IDPs is a quantitative and position-specific experimental characterization, and nuclear magnetic resonance (NMR) spectroscopy has emerged as the method of first choice. Here, we describe ho作者: 接觸 時(shí)間: 2025-3-30 08:31 作者: Asymptomatic 時(shí)間: 2025-3-30 13:25
Alexander Miguel Monzon,András Hatos,Marco Necci,Damiano Piovesan,Silvio C. E. Tosattonordnungen mit Gleichstrommaschinen sowie Drehfeldmaschinen mit Asynchron- und Synchronmaschinen. Sie werden anhand der Systemgleichungen, der Signalflusspl?ne sowie der Stellglied- und Regelungsvarianten diskutiert.? Am Ende des Buches finden sich zahlreiche übungsaufgaben mit L?sungen. .Das Lehrbu
