作者: NEEDY 時(shí)間: 2025-3-21 20:18
Intracellular Lipid Transport978-1-4939-9136-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Seizure 時(shí)間: 2025-3-22 00:24 作者: nitric-oxide 時(shí)間: 2025-3-22 04:34 作者: CESS 時(shí)間: 2025-3-22 10:58 作者: 嗎啡 時(shí)間: 2025-3-22 14:10
Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Proe yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.作者: 悠然 時(shí)間: 2025-3-22 20:19 作者: linear 時(shí)間: 2025-3-23 00:46
1064-3745 experts.This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or?at?membrane contact sites,作者: 減少 時(shí)間: 2025-3-23 03:07
Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell,l sphingosine to reveal its subcellular localization via crosslinking, fixation, and specific staining by click reaction with a fluorophore. Additionally, these probes allow investigations into lipid metabolism as revealed by thin-layer chromatography.作者: 同步左右 時(shí)間: 2025-3-23 08:23
Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transporte to assess not only the ER-to-mitochondria but also mitochondria-to-ER transports at the same time. We believe that this assay system can accelerate the research on inter-organelle phospholipid trafficking.作者: Deadpan 時(shí)間: 2025-3-23 16:12 作者: BROOK 時(shí)間: 2025-3-23 18:56
Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time,opy using the specific PS reporter C2.-GFP. We describe the construction of the required . yeast strain and the preparation of lyso-PS. We present an example of the use of this assay to follow the activity of the yeast PS transport proteins Osh6 and Osh7.作者: 獨(dú)白 時(shí)間: 2025-3-24 00:30 作者: 謙虛的人 時(shí)間: 2025-3-24 04:56 作者: 大喘氣 時(shí)間: 2025-3-24 06:53 作者: STELL 時(shí)間: 2025-3-24 10:41 作者: 議程 時(shí)間: 2025-3-24 17:11 作者: 微生物 時(shí)間: 2025-3-24 22:06
Monitoring and Modulating Intracellular Cholesterol Trafficking Using ALOD4, a Cholesterol-Binding this question. ALOD4 traps cholesterol at the PM, leading to depletion of ER cholesterol without altering total cell cholesterol. The effects of ALOD4 are reversible. This tool has been used to show that the ER is able to continuously sample cholesterol from PM, providing ER with information about levels of PM cholesterol.作者: 斜谷 時(shí)間: 2025-3-24 23:27 作者: Diluge 時(shí)間: 2025-3-25 06:59 作者: 大火 時(shí)間: 2025-3-25 10:36 作者: 閹割 時(shí)間: 2025-3-25 14:24
Yasushi Tamura,Rieko Kojima,Toshiya Endo geological, biol- ical, and technological processes and phenomena. All of these elements have been studied exhaustively, and voluminous material is available on their properties. Included in this material are numerous accounts of their electrochemical properties, ranging from reviews to extensive m作者: FORGO 時(shí)間: 2025-3-25 17:02 作者: 放縱 時(shí)間: 2025-3-25 22:57 作者: WAG 時(shí)間: 2025-3-26 03:34 作者: notification 時(shí)間: 2025-3-26 08:00
Neha Chauhan,Julian A. Jentsch,Anant K. Menonlvement in geological, biol- ical, and technological processes and phenomena. All of these elements have been studied exhaustively, and voluminous material is available on their properties. Included in this material are numerous accounts of their electrochemical properties, ranging from reviews to e作者: 最高峰 時(shí)間: 2025-3-26 08:57 作者: Override 時(shí)間: 2025-3-26 13:57
Shreya Endapally,Rodney E. Infante,Arun Radhakrishnan geological, biol- ical, and technological processes and phenomena. All of these elements have been studied exhaustively, and voluminous material is available on their properties. Included in this material are numerous accounts of their electrochemical properties, ranging from reviews to extensive m作者: 不如樂(lè)死去 時(shí)間: 2025-3-26 18:59
Yibin Lin,Lei Zheng,Mikhail Bogdanov geological, biol- ical, and technological processes and phenomena. All of these elements have been studied exhaustively, and voluminous material is available on their properties. Included in this material are numerous accounts of their electrochemical properties, ranging from reviews to extensive m作者: 逗留 時(shí)間: 2025-3-26 22:31 作者: IDEAS 時(shí)間: 2025-3-27 04:48
Xin Bian,Pietro De Camilli geological, biol- ical, and technological processes and phenomena. All of these elements have been studied exhaustively, and voluminous material is available on their properties. Included in this material are numerous accounts of their electrochemical properties, ranging from reviews to extensive m作者: vitreous-humor 時(shí)間: 2025-3-27 06:39 作者: GRAIN 時(shí)間: 2025-3-27 11:23 作者: 易受刺激 時(shí)間: 2025-3-27 15:02 作者: interference 時(shí)間: 2025-3-27 18:13 作者: Admonish 時(shí)間: 2025-3-28 01:45 作者: Pigeon 時(shí)間: 2025-3-28 03:17 作者: 爭(zhēng)吵加 時(shí)間: 2025-3-28 08:07 作者: 疏忽 時(shí)間: 2025-3-28 13:30
Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time,pecies in cells. We present here a microfluidics-based protocol to follow the transport of phosphatidylserine (PS) in yeast cells from the site of its synthesis, the endoplasmic reticulum (ER), to downstream compartments, primarily the plasma membrane under our conditions. This assay takes advantage作者: Little 時(shí)間: 2025-3-28 17:39 作者: Commemorate 時(shí)間: 2025-3-28 19:52 作者: 猛烈責(zé)罵 時(shí)間: 2025-3-29 01:35 作者: 節(jié)省 時(shí)間: 2025-3-29 05:28
Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell,f living cells, since fluorescent modifications drastically change lipid properties. Here, we describe the use of bifunctional as well as trifunctional sphingosine to reveal its subcellular localization via crosslinking, fixation, and specific staining by click reaction with a fluorophore. Additiona作者: 緯線 時(shí)間: 2025-3-29 08:23
Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Proe yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.作者: Kidney-Failure 時(shí)間: 2025-3-29 13:38
Measurement of Intracellular Sterol Transport in Yeast,sfer it to another across the cytoplasm. Here we describe a suite of complementary assays to measure intracellular sterol transport in the model eukaryote ., as well as to quantify protein-mediated sterol transport between populations of vesicles in vitro. The in vivo assays can be adapted to study 作者: 過(guò)度 時(shí)間: 2025-3-29 17:57 作者: STING 時(shí)間: 2025-3-29 23:44 作者: set598 時(shí)間: 2025-3-30 00:38 作者: 五行打油詩(shī) 時(shí)間: 2025-3-30 04:35 作者: Amendment 時(shí)間: 2025-3-30 11:13 作者: 輕信 時(shí)間: 2025-3-30 12:41
Determining the Lipid-Binding Specificity of SMP Domains: An ERMES Subunit as a Case Study,. The yeast Endoplasmic Reticulum–Mitochondrion Encounter Structure (ERMES) complex is composed of five subunits that tether the ER and mitochondria. Three ERMES subunits (i.e., Mdm12, Mmm1, and Mdm34) contain the synaptotagmin-like mitochondrial lipid-binding protein (SMP) domain. The SMP domain be作者: Herpetologist 時(shí)間: 2025-3-30 17:00
In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Membertracking of the lipid. Typically, direct tracking involves lipid labeling via attachment of fluorophores or introduction of radioactivity. Members of the GlycoLipid Transfer Protein (GLTP) superfamily are used to illustrate two broadly applicable methods for direct lipid tracking. One method relies 作者: 好忠告人 時(shí)間: 2025-3-30 21:00 作者: 異常 時(shí)間: 2025-3-31 03:42
In Vitro Strategy to Measure Sterol/Phosphatidylinositol-4-Phosphate Exchange Between Membranes,ily of lipid transfer proteins (LTPs), conserved among eukaryotes, are not all mere sterol transporters or sensors. Indeed, some of them are able to exchange sterol for phosphatidylinositol-4-phosphate (PI4P) or phosphatidylserine (PS) for PI4P between membranes and thereby to use PI4P metabolism to作者: 巫婆 時(shí)間: 2025-3-31 06:16 作者: phytochemicals 時(shí)間: 2025-3-31 09:22
Monitoring Non-vesicular Transport of Phosphatidylserine and Phosphatidylinositol 4-Phosphate in Inbly tethered to the ER and also dynamically interacts with PM PI4P/PI(4,5)P. via its pleckstrin homology (PH) domain. We show that this method is able to detect PI4P and PS changes in the PM upon acute recruitment of an ORP5 construct to the PM. This method is convenient and robust, utilizing popula作者: projectile 時(shí)間: 2025-3-31 14:53
Intracellular and Plasma Membrane Cholesterol Labeling and Quantification Using Filipin and GFP-D4,. Second, to optically distinguish and quantify intracellular cholesterol accumulation, we have adapted the classical filipin cholesterol staining protocol. Indeed, we observed that treatment of living cells with methyl-β-cyclodextrin, a chemical known to extract cholesterol from the plasma membrane作者: Capture 時(shí)間: 2025-3-31 21:25 作者: asthma 時(shí)間: 2025-4-1 00:02
Determining the Lipid-Binding Specificity of SMP Domains: An ERMES Subunit as a Case Study,using lipid displacement and liposome flotation in vitro assays with liposomes as model biological membranes. These methods can be applied to the study and characterization of novel lipid-binding and lipid-transfer proteins.作者: 服從 時(shí)間: 2025-4-1 04:47
In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Member nanodiscs), (2) involving other lipid types by other lipid transfer proteins, (3) with protein preparations that are either crudely or highly purified, and (4) that is spontaneous and occurs in the absence of protein.作者: 得意人 時(shí)間: 2025-4-1 09:43
In Vitro Strategy to Measure Sterol/Phosphatidylinositol-4-Phosphate Exchange Between Membranes,milies have such an exchange activity. Besides, this protocol is amenable to test whether molecules can act as Orphilins, which have been found to inhibit the sterol/PI4P exchange activity of certain ORPs. Last, our strategy to measure in real-time PI4P transport using a known lipid-binding domain c作者: flammable 時(shí)間: 2025-4-1 12:32 作者: sphincter 時(shí)間: 2025-4-1 14:50 作者: 結(jié)果 時(shí)間: 2025-4-1 21:06
Chi-Lun Chang,Jen Liou active site structure discussions. In terms of catalyst performance, while fuel cell activity for heat-treated non-PGM catalysts has been dramatically improved over the past two decades, further improvements are still required to compete with state-of-the-art Pt catalysts. Though indispensable, the作者: Freeze 時(shí)間: 2025-4-2 02:16 作者: PET-scan 時(shí)間: 2025-4-2 05:19
Yasushi Tamura,Rieko Kojima,Toshiya Endosolution at the fundamental level is surprising and somewhat troubling. It is troubling in the sense that the non-photoelectrochemistry of silicon seems “to have fallen through the cracks,” with the result that some of the electrochemical properties of this element are not as well known as might be
