作者: 出處 時(shí)間: 2025-3-21 21:51 作者: 沒(méi)有希望 時(shí)間: 2025-3-22 01:57
Paloma Ordó?ez-MoránIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 睨視 時(shí)間: 2025-3-22 05:29
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/473066.jpg作者: adhesive 時(shí)間: 2025-3-22 12:32
https://doi.org/10.1007/978-1-0716-0747-3Stem cell differentiation; Cell renewal; Intestinal tumor-initiating cells; Intestinal crypts; Gastroint作者: LAP 時(shí)間: 2025-3-22 16:25 作者: 毀壞 時(shí)間: 2025-3-22 17:31 作者: Small-Intestine 時(shí)間: 2025-3-23 01:05
Book 2020dying intestinal stem cells properties. Beginning with a section on .in vitro. techniques to study different aspects of the intestinal stem cell?functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the i作者: Mnemonics 時(shí)間: 2025-3-23 04:45 作者: compose 時(shí)間: 2025-3-23 08:29 作者: Tartar 時(shí)間: 2025-3-23 10:46 作者: HILAR 時(shí)間: 2025-3-23 15:54
Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategyelial organoids generated from patient-derived tissues, from induced pluripotent stem cell (iPSC) derived intestinal organoids, and from freshly isolated patient tissue intestinal crypts. These methods will facilitate experimental analysis of human LGR5-expressing cells in development, wound healing, and cancer.作者: 地名詞典 時(shí)間: 2025-3-23 20:36 作者: Cardiac-Output 時(shí)間: 2025-3-24 01:44 作者: 發(fā)生 時(shí)間: 2025-3-24 04:54 作者: 裙帶關(guān)系 時(shí)間: 2025-3-24 06:48
Autophagy Detection in Intestinal Stem Cellsng autophagosomes in GFP-LC3 transgenic mice and quantifying the p62 protein levels. We also describe methods for detecting mitophagy in these cells, by analyzing the mitochondrial transmembrane potential and reactive oxygen species (ROS) level by MitoTracker and CellROX solution, respectively.作者: 我沒(méi)有命令 時(shí)間: 2025-3-24 12:46 作者: 善辯 時(shí)間: 2025-3-24 18:39 作者: 魯莽 時(shí)間: 2025-3-24 19:36 作者: COST 時(shí)間: 2025-3-25 01:03 作者: Mitigate 時(shí)間: 2025-3-25 07:02
Analysis of Aged Dysfunctional Intestinal Stem Cellss that depend on stem cell in the adult. Intestine is such an organ, and upon aging intestinal regeneration is impaired due to decline of intestinal stem cell function. To determine the aging status of intestine and intestinal stem cells, histological analyses; analyses of the level of proliferation作者: murmur 時(shí)間: 2025-3-25 09:20 作者: 令人悲傷 時(shí)間: 2025-3-25 13:35 作者: AVID 時(shí)間: 2025-3-25 19:20 作者: Subdue 時(shí)間: 2025-3-25 20:00
Autophagy Detection in Intestinal Stem Cellsagy in intestinal stem cells (ISCs) is important for ISC homeostasis. Here we describe the detailed methods for detecting autophagy in ISCs by observing autophagosomes in GFP-LC3 transgenic mice and quantifying the p62 protein levels. We also describe methods for detecting mitophagy in these cells, 作者: 不開心 時(shí)間: 2025-3-26 01:50
Single-Cell Transcriptional Profiling of the Intestinal Epitheliumution. By profiling the set of expressed transcripts in each cell, single-cell transcriptomics has allowed for the cataloging of the cellular constituents of multiple organs and tissues, both in health and disease. In addition, these technologies have provided mechanistic insights into cellular func作者: 袋鼠 時(shí)間: 2025-3-26 07:11 作者: 昏暗 時(shí)間: 2025-3-26 09:46
Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance ofem cells. Here, we describe a transient gene expression method for the production of these proteins from human embryo kidney 293 (HEK293) cells cultivated in suspension using orbitally shaken bioreactors. Plasmid DNA was delivered into cells using the cationic polymer polyethylenimine (PEI). The 7-d作者: Grandstand 時(shí)間: 2025-3-26 13:08
In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenancend associated diseases. Herein, we present a method for renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial cell proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific ste作者: QUAIL 時(shí)間: 2025-3-26 17:14 作者: Conquest 時(shí)間: 2025-3-26 21:31
Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derivedn their usability is the need for donor tissue fragments or pluripotent stem cells to generate the organoids. Here, we describe an approach to generate intestinal organoids from fibroblasts, a new source. We used direct reprogramming technology to generate cells with the properties of fetal intestin作者: 北極人 時(shí)間: 2025-3-27 02:01 作者: FORGO 時(shí)間: 2025-3-27 05:50 作者: atopic-rhinitis 時(shí)間: 2025-3-27 11:40
Kodandaramireddy Nalapareddy,Hartmut Geigere analysis of the impact of electoral institutions on political outcomes. Before delving into the empirical evaluation of electoral participation, it is worthwhile pausing to consider the normative interpretation of aggregate voting levels. Electoral participation is generally understood as being re作者: Biguanides 時(shí)間: 2025-3-27 13:59
Chia-Wei Cheng,Omer H. Yilmaz,Maria M. Mihaylovastablished democracies, most of which have historically been located in Western Europe.. Research has also been undertaken on the comparative study of electoral regulations such as nomination procedures and campaign financing.. Yet it is not obvious that the effects of given electoral institutions i作者: Dendritic-Cells 時(shí)間: 2025-3-27 17:51
Irina A. Okkelman,Jens Puschhof,Dmitri B. Papkovsky,Ruslan I. Dmitrievw entrants into the electoral arena to penetrate the support bases of existing political organizations. Stability and change in party systems have long fascinated political scientists, but the nature of the research questions they have asked has been conditioned by the cases they have taken as their作者: 繁榮地區(qū) 時(shí)間: 2025-3-27 22:48 作者: glucagon 時(shí)間: 2025-3-28 03:43 作者: 柳樹；枯黃 時(shí)間: 2025-3-28 07:56
stablished democracies, most of which have historically been located in Western Europe.. Research has also been undertaken on the comparative study of electoral regulations such as nomination procedures and campaign financing.. Yet it is not obvious that the effects of given electoral institutions i作者: STRIA 時(shí)間: 2025-3-28 13:39 作者: Living-Will 時(shí)間: 2025-3-28 15:28
Maxim Norkin,Claudia Capdevila,Ruben I. Calderon,Tianhong Su,Maria Trifas,Paloma Ordó?ez-Morán,Kellety rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: Iniquitous 時(shí)間: 2025-3-28 22:21
ty rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: 創(chuàng)新 時(shí)間: 2025-3-29 01:35
David L. Hacker,Paloma Ordó?ez-Moránty rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: LATHE 時(shí)間: 2025-3-29 04:42
Tomohiro Mizutani,Hans Cleversty rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: Hamper 時(shí)間: 2025-3-29 11:03
Simon Vales,Holly M. Poling,Nambirajan Sundaram,Michael A. Helmrath,Maxime M. Mahety rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: 疏遠(yuǎn)天際 時(shí)間: 2025-3-29 12:18
Chathruckan Rajendra,Tomas Wald,Kevin Barber,Jason R. Spence,Faranak Fattahi,Ophir D. Kleinty rather than electrostriction, conduction, electromechanical effects, or the motion of conductors in the field of space charges. Two distinct types of polymers can be piezoelectric. Amorphous polymers are piezo- and pyroelectric by virtue of a nonequilibium but kinetically stable net dipole orient作者: Graves’-disease 時(shí)間: 2025-3-29 18:11 作者: 典型 時(shí)間: 2025-3-29 20:53 作者: 頌揚(yáng)國(guó)家 時(shí)間: 2025-3-30 01:14
Pierre Dessen,Joerg Huelsken,Paloma Ordó?ez-Moránbiotechnology and other branches. It is characterized by specific methods of experimental investigations based on recording charge transfer, polarization and depolarization of dielectrics and involves original techniques and physico-mathematical aids where notions that exist at the interface of seve作者: 期滿 時(shí)間: 2025-3-30 06:16 作者: META 時(shí)間: 2025-3-30 11:51 作者: spondylosis 時(shí)間: 2025-3-30 16:05 作者: 可行 時(shí)間: 2025-3-30 17:53
Laura E. Sanman,Ina W. Chen,Jake M. Bieber,Curtis A. Thorne,Lani F. Wu,Steven J. Altschuler is a matter of rule of the people, the more people who participate in a political system, the more democratic it may be held to be (provided that participation has a meaningful impact on democratic outcomes). In this sense a high level of mass participation can be seen as a necessary — if not suffi
