作者: 令人心醉 時(shí)間: 2025-3-21 20:35
Innate Immune Activation978-1-4939-7519-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 出處 時(shí)間: 2025-3-22 01:03 作者: invulnerable 時(shí)間: 2025-3-22 04:35 作者: nepotism 時(shí)間: 2025-3-22 10:50
Dominic De Nardo,Christine M De NardoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: 音的強(qiáng)弱 時(shí)間: 2025-3-22 16:52 作者: explicit 時(shí)間: 2025-3-22 18:15
https://doi.org/10.1007/978-1-4939-7519-8NFB activation; macrophage activation; CRISPR; endosomal TLRs; NFB作者: Hiatal-Hernia 時(shí)間: 2025-3-22 21:38 作者: 啜泣 時(shí)間: 2025-3-23 03:26 作者: 逗留 時(shí)間: 2025-3-23 06:24
Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System,oetic cells with components required for this process, we have adopted a lentiviral system for delivery of the CRISPR/Cas9 components into myeloid cell lines. Here, we detail the process of knocking out genes from pools of cultured myeloid cells using this CRISPR/Cas9 system and describe methods of 作者: FLAGR 時(shí)間: 2025-3-23 13:32 作者: hurricane 時(shí)間: 2025-3-23 14:39 作者: 粘 時(shí)間: 2025-3-23 21:15
Biochemical Isolation of the Myddosome from Murine Macrophages,pathways essential for host defense. Despite their structural divergence, key signaling molecules in diverse innate immune pathways mediate signal transduction by assembling higher-order protein complexes at specific subcellular locations in a stimulus-dependent manner. These protein complexes are c作者: 大吃大喝 時(shí)間: 2025-3-24 01:01 作者: esculent 時(shí)間: 2025-3-24 03:29 作者: 下邊深陷 時(shí)間: 2025-3-24 09:04
Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis,l inflammasome activation or caspase-4, 5, and 11 upon their ligation and activation by cytosolic LPS. Upon a single cleavage between the two domains in Gasdermin-D, the N-terminal domain binds to membrane lipids and lyses cells by forming pores of an inner diameter of 10–14 nm within the membrane. 作者: 缺乏 時(shí)間: 2025-3-24 13:33
Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking,flammasomes. This unique feature of ASC allows its visualization by several imaging techniques and constitutes a reliable and feasible readout for inflammasome activation in cells and tissues. We have previously described step-by-step protocols to generate immortalized cell lines stably expressing A作者: Bricklayer 時(shí)間: 2025-3-24 15:15 作者: 租約 時(shí)間: 2025-3-24 19:44 作者: Brocas-Area 時(shí)間: 2025-3-24 23:31 作者: engrave 時(shí)間: 2025-3-25 03:42 作者: Coronary 時(shí)間: 2025-3-25 10:26
,Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISermed pyroptosis, pro-inflammatory mediators that lack canonical secretory signal sequences, such as interleukin-1β (IL-1β), are released into the extracellular environment. To define whether cell death is required for the release of IL-1β, or if IL-1β can be actively secreted from viable cells, we 作者: macabre 時(shí)間: 2025-3-25 14:05 作者: 蘆筍 時(shí)間: 2025-3-25 19:44
To be able to discover and to extract knowledge from data is a task that many researchers and practitioners are endeavoring to accomplish. There is a lot of hidden knowledge waiting to be discovered – this is the challenge created by today’s abundance of data..Data Mining and Knowledge Discovery Han作者: 捐助 時(shí)間: 2025-3-25 23:15 作者: 走調(diào) 時(shí)間: 2025-3-26 02:27
Thomas Ulas,Joachim L. Schultze,Marc Beyerdicine for example, modeling complex biological systems requires linking knowledge acrossmulti-level of science, fromgenes to disease. Themove to multilevel research requires new strategies; in this discussion we present ., a novel methodology for linking published research findings..The development作者: 記憶法 時(shí)間: 2025-3-26 04:38 作者: nutrition 時(shí)間: 2025-3-26 08:37
Moritz M. Gaidt,Francesca Rapino,Thomas Graf,Veit Hornungrmation and database technologies. With the increasing use of databases the need to be able to digest large volumes of data being generated is now critical. It is estimated that only 5%-10% of commercial databases have ever been analysed [23]. As Massey and Newing [24] indicated that database techno作者: APO 時(shí)間: 2025-3-26 12:56
Orna Ernst,Sharat J. Vayttaden,Iain D. C. Fraser Ph.D.ed from a type of analysis that extracts information from coincidence [ Blaxton andWestphal, 1998]. Sometimes called., this methodology allows a data analyst to discover correlations, or co-occurrences of transactional events. In the classic example, consider the items contained in a customer’s shop作者: Culmination 時(shí)間: 2025-3-26 17:07
Yunhao Tan,Jonathan C. Kaganed from a type of analysis that extracts information from coincidence [ Blaxton andWestphal, 1998]. Sometimes called., this methodology allows a data analyst to discover correlations, or co-occurrences of transactional events. In the classic example, consider the items contained in a customer’s shop作者: ovation 時(shí)間: 2025-3-27 00:16 作者: 甜得發(fā)膩 時(shí)間: 2025-3-27 02:52 作者: Vertebra 時(shí)間: 2025-3-27 07:49 作者: 文字 時(shí)間: 2025-3-27 11:13 作者: Allowance 時(shí)間: 2025-3-27 17:22
Julien Moretti,Nicolas Vabret,J. Magarian Blanderts, and actions resulting in the intervention of VAR in European soccer leagues referees. The game of soccer at the elite level is characterised by intense competitions, a high level of intensity, technical, and tactical skills coupled with a long duration of play. Referees are required to officiate作者: 中古 時(shí)間: 2025-3-27 21:07 作者: MEAN 時(shí)間: 2025-3-27 22:24 作者: 情節(jié)劇 時(shí)間: 2025-3-28 03:22 作者: 被詛咒的人 時(shí)間: 2025-3-28 07:43
Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking,linking of ASC followed by immunoblotting. These methods allow for the discrimination of inflammasome-activated versus non-activated cells, the identification of lineage-specific inflammasome activation in complex cell mixtures, and sorting of inflammasome-activated cells for further analysis.作者: legacy 時(shí)間: 2025-3-28 12:18 作者: BROOK 時(shí)間: 2025-3-28 15:56 作者: Colonnade 時(shí)間: 2025-3-28 20:26 作者: 中國(guó)紀(jì)念碑 時(shí)間: 2025-3-28 23:05
Bioinformatic Assessment of Macrophage Activation by the Innate Immune System,f CD14. monocytes into macrophages followed by in vitro stimulations to identify stimulation-specific gene modules. These gene modules can be used for experimental validation, as well as further bioinformatic analysis to determine key pathways or upstream transcription factors.作者: 淺灘 時(shí)間: 2025-3-29 06:28
Generation of Innate Immune Reporter Cells Using Retroviral Transduction, of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.作者: 挑剔小責(zé) 時(shí)間: 2025-3-29 09:00 作者: gentle 時(shí)間: 2025-3-29 13:02
Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis,The inter-domain cleavage of Gasdermin-D is a reliable marker for the activation of inflammatory caspases and cell pyroptosis. Here, we describe the methods for examining Gasdermin-D cleavage by activated inflammatory caspases in vitro and upon inflammasome activation in vivo.作者: 橡子 時(shí)間: 2025-3-29 18:16 作者: 缺陷 時(shí)間: 2025-3-29 22:36 作者: admission 時(shí)間: 2025-3-30 02:53
Olaf-Oliver Wolz,Manfred Koegl,Alexander N. R. Weberg evolutionary algorithms to data mining is that evolutionary algorithms are robust search methods which perform a global search in the space of candidate solutions (rules or another form of kn978-3-642-07763-0978-3-662-04923-5Series ISSN 1619-7127 Series E-ISSN 2627-6461 作者: 有角 時(shí)間: 2025-3-30 06:25
Roland Felix Dreier,José Carlos Santos,Petr Broz Ph.D.作者: 口味 時(shí)間: 2025-3-30 10:52
Stephanie A. Conos Ph.D,Lisa M. Lindqvist Ph.D.,James E. Vince Ph.D.作者: FID 時(shí)間: 2025-3-30 12:45
,Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1,ersion from malignant B-lineage cells to monocytes/macrophages is caused by the inducible nuclear translocation of a C/EBPα transgene, BLaER1 cells. Using this model, we were able to characterize a novel inflammasome signaling entity that could not have been uncovered in the murine system or THP-1 c作者: FID 時(shí)間: 2025-3-30 17:13
Biochemical Isolation of the Myddosome from Murine Macrophages,ereby leading to NF-?B and AP-1 activation, and the subsequent expression of pro-inflammatory cytokines. Herein, we provide a detailed protocol for studying myddosome assembly in murine bone marrow-derived macrophages (BMDMs).作者: 消息靈通 時(shí)間: 2025-3-30 23:23
Examining Myddosome Formation by Luminescence-Based Mammalian Interactome Mapping (LUMIER),-receptor complex and its role in innate immunity, such as its regulation by posttranslational modification. Compared to structural and biochemical analyses, luminescence-based mammalian interactome mapping (LUMIER) is a straightforward, automatable, quantifiable, and versatile technique to study pr作者: 主動(dòng)脈 時(shí)間: 2025-3-31 01:18 作者: 山間窄路 時(shí)間: 2025-3-31 05:31 作者: 難管 時(shí)間: 2025-3-31 11:56 作者: 創(chuàng)作 時(shí)間: 2025-3-31 17:03
h in-depth descriptions of data mining applications in various interdisciplinary industries including finance, marketing, medicine, biology, engineering, telecommunications, software, and security..Data Mining and Knowledge Discovery Handbook, Second Edition. is designed for research scientists, lib作者: 與野獸博斗者 時(shí)間: 2025-3-31 18:17 作者: Anticlimax 時(shí)間: 2025-4-1 01:11
Thomas Ulas,Joachim L. Schultze,Marc Beyery manifestations of these diseases across multiple biological and behavioral levels (i.e., genotypes and phenotypes at levels from molecular expression through complex syndromes). Phenomics – the study of phenotypes on a genome-wide scale – requires close collaboration among specialists in multiple 作者: 羞辱 時(shí)間: 2025-4-1 04:30
Paul J. Baker,Seth L. Masters Ph.D.y, artificial intelligence, pattern recognition, and causal relationship discovery, as well as signal processing. It also provides a framework for integrating plant operators and supervisors into the design of process monitoring and control systems.978-1-4471-1137-5978-1-4471-0421-6Series ISSN 1430-9491 Series E-ISSN 2193-1577 作者: 彎彎曲曲 時(shí)間: 2025-4-1 06:14
Moritz M. Gaidt,Francesca Rapino,Thomas Graf,Veit Hornungto help in decision making (Figure 2.1). A widely accepted definition is given by Fayyad et al. [25] in which KDD is defined as the non-trivial process of identifying valid, novel, potentially useful, and ultimately understandable patterns in data. The definition regards KDD as a complicated process作者: DEFER 時(shí)間: 2025-4-1 12:56 作者: effrontery 時(shí)間: 2025-4-1 14:29
