作者: Negligible 時(shí)間: 2025-3-22 00:11 作者: 假裝是我 時(shí)間: 2025-3-22 02:48 作者: 劇毒 時(shí)間: 2025-3-22 06:56 作者: deactivate 時(shí)間: 2025-3-22 09:39 作者: 英寸 時(shí)間: 2025-3-22 12:54
Wenyan Wang,Bruce A. Malcolmh was to last almost to the end of the war in Europe in summer 1945. Naples was liberated in October 1943, Rome in June 1944, Florence in August, while the general rising of the Italian partisans that drove the Germans out of the cities of the Po valley, was brought to a victorious conclusion on 25 作者: conjunctivitis 時(shí)間: 2025-3-22 18:31 作者: 發(fā)展 時(shí)間: 2025-3-23 01:14 作者: Popcorn 時(shí)間: 2025-3-23 05:20 作者: Cardiac-Output 時(shí)間: 2025-3-23 07:29 作者: ACME 時(shí)間: 2025-3-23 09:45
Lucio Gama,Gerda E. Breitwieseritics in the world outside them. As we suggested in the first chapter, however, comparative communism should properly be thought of as a subfield of comparative politics rather than as a substitute for that method of inquiry, and if we are interested in examining the performance of the communist sta作者: 預(yù)感 時(shí)間: 2025-3-23 16:10
David M. Young,Ruben G. Kok,L. Nicholas Ornston go beyond influencing state activities, and secure a tangible input into the activities of business organizations as well. The educative, productive and protective aspects of communitarian citizenship need to be supported by businesses, not just in terms of their compliance with legislation, but al作者: 迅速飛過(guò) 時(shí)間: 2025-3-23 18:04
Ross N. Nazar,P. D. Abeyrathne,Robert V. A. Intinese-knit communities which are neither possible to recreate, nor indeed desirable, as they could be highly oppressive. Philosophical discussions of the relationships between individuals and communities have not been of much help in clarifying this matter as they have remained at generally an abstract作者: Extemporize 時(shí)間: 2025-3-23 23:08
Dario E. Lehoux,Roger C. Levesquethe power of culture over nature, and hostility between them. There is a way of framing existence that addresses the respect we have learned for nature and the mystery of our sense of it. Nature not only comprises humanity, living things, and objects but also the way human beings act in producing th作者: mettlesome 時(shí)間: 2025-3-24 04:20 作者: adroit 時(shí)間: 2025-3-24 10:34
Jennifer A. Maynard,Gang Chen,George Georgiou,Brent L. Iversont from each other as Britain’s and the USA’s have in practice included a range of welfare provisions, some services being directly provided by the state whilst others have been provided through the private market or through voluntary organisations. Typically, for the personal social services, the mo作者: 同來(lái)核對(duì) 時(shí)間: 2025-3-24 12:47 作者: 激怒某人 時(shí)間: 2025-3-24 16:53 作者: 陰謀 時(shí)間: 2025-3-24 20:05 作者: d-limonene 時(shí)間: 2025-3-25 00:20
Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity,.) developed a general method for the introduction of specific changes in DNA sequence, which involves hybridization of a synthetic oligonucleotide (ON) containing the desired mutation to a single-stranded DNA (ssDNA) target template. Following hybridization, the oligonucleotide is extended with a D作者: 隱語(yǔ) 時(shí)間: 2025-3-25 04:41 作者: Neonatal 時(shí)間: 2025-3-25 10:16 作者: Analogy 時(shí)間: 2025-3-25 12:59
Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids,xtensively explored, as illustrated by several chapters in this volume. In particular, the relative ease with which DNA fragments of 2–3 kb can be generated facilitated the development of the highly efficient procedure known as “recombination PCR” (RPCR), the principles of which were first described作者: Lipoma 時(shí)間: 2025-3-25 17:42 作者: 嚙齒動(dòng)物 時(shí)間: 2025-3-25 22:23 作者: 吵鬧 時(shí)間: 2025-3-26 03:01
Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA,have been described, these methods include such drawbacks as the possibility of generating undesired secondary mutations, because of the low fidelity of some of the thermostable DNA polymerases, the need for four or more specific oligonucleotide primers, and the use of sophisticated, individually op作者: 轉(zhuǎn)換 時(shí)間: 2025-3-26 04:54 作者: 偽善 時(shí)間: 2025-3-26 11:10 作者: prosthesis 時(shí)間: 2025-3-26 13:20
Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction, a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After the advent of polymerase chain reaction . mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic Kunkel’s method (.).作者: 榮幸 時(shí)間: 2025-3-26 18:14 作者: curriculum 時(shí)間: 2025-3-27 00:59 作者: Migratory 時(shí)間: 2025-3-27 03:18 作者: Electrolysis 時(shí)間: 2025-3-27 05:40
High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction,Often, it is desirable to subject the protein or gene of interest to scanning mutagenesis. The genetic analysis of scores of single or multiple point mutations contributes to a better understanding of . elements in gene expression (.). In the case of proteins, cysteine (Cys) (.,.) and alanine scanni作者: Malfunction 時(shí)間: 2025-3-27 09:59 作者: ostrish 時(shí)間: 2025-3-27 16:44
Random Transposon Mutagenesis of Large DNA Molecules in ,, reasons. Because of their large size (up to 300 kbp), unique restriction enzyme sites are usually not available. Even if they are, modifications based on restriction digest and ligation are mastered only by scientists with extensive experience in handling large DNA molecules. Moreover, sequence inf作者: flourish 時(shí)間: 2025-3-27 20:03
Random Chromosomal Gene Disruption Using Cassette Mutagenesis,sequence information (e.g., over the past 5 yr there have been 30 published microbial genomes and over 100 microbial genome sequencing projects), there is a growing need for reliable methods of random DNA mutagenesis to allow for functional genomics work. The authors were studying ., the first organ作者: 使閉塞 時(shí)間: 2025-3-27 22:46 作者: 承認(rèn) 時(shí)間: 2025-3-28 06:06 作者: 軟弱 時(shí)間: 2025-3-28 07:14 作者: Assemble 時(shí)間: 2025-3-28 11:17 作者: Admire 時(shí)間: 2025-3-28 16:05
In Vitro Scanning-Saturation Mutagenesis,). However, a stumbling block to the wider application of these techniques is that each desired mutation must be introduced into a gene, cloned into bacteria, and the protein produced then purified (.). This is a resource and time intensive process that precludes routine use.作者: Hyaluronic-Acid 時(shí)間: 2025-3-28 20:36
1064-3745 r biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventiona作者: 清唱?jiǎng)?nbsp; 時(shí)間: 2025-3-28 22:54 作者: 廢墟 時(shí)間: 2025-3-29 06:50
High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction,ng mutagenesis (.) are popular means for investigating the structure and function of proteins and the interactions between proteins. Cys-scanning mutagenesis is particularly useful, since the introduction of Cys allows targeted chemical modification with a wide variety of sulfhydryl-reactive reagents (.,.).作者: Ingredient 時(shí)間: 2025-3-29 09:16 作者: GLEAN 時(shí)間: 2025-3-29 13:11 作者: 痛得哭了 時(shí)間: 2025-3-29 17:26 作者: Campaign 時(shí)間: 2025-3-29 22:27
Book 20022nd editiontechnique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers...Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today‘s molecular biologists a rich compendium of reliable and powerful tec作者: 巧辦法 時(shí)間: 2025-3-30 01:53
Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity,of Kunkel (.), Eckstein (.), and Deng (.,.) employ negative selection against the wild-type DNA strand, in which the parental DNA is selectively degraded, either by growth in an alternate host strain, or by digestion with a nuclease or restriction enzyme. The methods of Lewis and Thompson (.) and Bo作者: Obstacle 時(shí)間: 2025-3-30 04:27
Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids,d by recombination in vivo following cotransformation of the two fragments into competent recA.. cells. Since both DNA strands carry the desired mutation as directed by the mutagenic primers, the recovery of mutant clones should, in theory, approach 100%, although, in practice, mutant yields, rangin作者: thyroid-hormone 時(shí)間: 2025-3-30 11:47
Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product,utant products at a frequency of less than 10%. The reason for this low efficiency may stem from the nucleotide sequence of the target DNA, which may acquire stable secondary structures, such as stem-loops. These nucleotide structures may interfere with the annealing of the mutagenic primers, and co作者: 狂亂 時(shí)間: 2025-3-30 14:02
Site-Directed Mutagenesis by Polymerase Chain Reaction,tion enzyme marker site is introduced into the target gene by silent mutations, to allow fast and convenient screening for the presence of the mutation (.,.,.). Because random mutagenesis and directed evolution are being widely used for protein engineering (.), a method for random mutagenesis by PCR作者: 乞討 時(shí)間: 2025-3-30 17:49
Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene Aftction (PCR) amplification. Observed changes in phenotype can be correlated to changes in nucleotide sequence, and thus permit a general survey of how changes in structure influence function in the gene product. Alternatively, nucleotide substitution can be used to alter the substrate specificity or 作者: Congregate 時(shí)間: 2025-3-30 21:39
Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis,Tn mutants, defective in virulence, which cannot be maintained in vivo. Attenuated mutants are selected and retested to confirm attenuation; disrupted genes are cloned via the Tn marker, and the inactivated gene confirmed by DNA sequencing. Modifications of STM, allowing rapid and easy identificatio作者: 混合,攙雜 時(shí)間: 2025-3-31 01:17
Random Chromosomal Gene Disruption Using Cassette Mutagenesis,plementation of transformation-deficient mutants derived by chemical mutagenesis (.), a “poison-DNA” selection method (.), mini-transposon (Tn)10 mutagenesis (.), Tn. mutagenesis (.), and other genetic selection techniques (.). In order to identify the remaining transformation genes by insertional m作者: 彩色的蠟筆 時(shí)間: 2025-3-31 05:57
1961; Mote, 1965; Churchward, 1966; Clarke, 1967; Gilison, 1968; Jacobs, 1970; Hill, 1972, 1973, 1976a; Friedgut, 1979; Zaslavsky and Brym, 1978, 1983; White, forthcoming). In Eastern Europe, where elections offer a potentially more fruitful field for research, treatment has been piecemeal: only Po作者: 沙文主義 時(shí)間: 2025-3-31 12:46
Priya Handa,Swapna Thanedar,Umesh Varshneyistice was announced the King and the government fled from Rome to seek Allied protection and were settled in Bari. The Germans reacted to the Armistice by occupying the non-liberated part of the country, and, having rescued Mussolini, they set him up in a puppet regime: the Republic of Salò.作者: 儲(chǔ)備 時(shí)間: 2025-3-31 16:22 作者: 密碼 時(shí)間: 2025-3-31 18:40
Fusheng Li,James I. Mullinsnning of 1944. Stockholm, Lisbon, Ankara and Istanbul were among the places where emissaries of both Mihai Antonescu and the democratic opposition paved the way for Romania’s volte-face. The main architects of the subtle and complex structure of diplomatic and military plans aiming at extricating Ro作者: ULCER 時(shí)間: 2025-4-1 00:33
Yang-Gyun Kim,Stefan Maasistice was announced the King and the government fled from Rome to seek Allied protection and were settled in Bari. The Germans reacted to the Armistice by occupying the non-liberated part of the country, and, having rescued Mussolini, they set him up in a puppet regime: the Republic of Salò.作者: 易于出錯(cuò) 時(shí)間: 2025-4-1 02:00 作者: 懸掛 時(shí)間: 2025-4-1 08:46 作者: enchant 時(shí)間: 2025-4-1 13:55
Grace DeSantis,J. Bryan Jones well as the appearance of psychology careers in Argentina, which altered the dynamics of the “psy” field. Both political and disciplinary changes led to the division of Argentine Pavlovism into an orthodox and a heterodox group, with different clinical proposals and ideological positions.作者: lavish 時(shí)間: 2025-4-1 17:13
Xueni Chen,Weimin Liu,Ileana Quinto,Giuseppe Scala shows how the efforts of this group contributed to the diffusion of communist scientific ideas and practices in South America as part of a transnational circuit of communist scholars and intellectuals that included France, Spain and the USA, which fostered scientific exchange and politicized scienc作者: 腫塊 時(shí)間: 2025-4-1 21:57
Jesper Br?s-Poulsen,Jane N?hr,Leif Kongskov Larsens, in the early 1990s, a classification that was less easy to defend, and we have accordingly identified the states concerned as ‘communist and postcommunist’ in Table 1.1. All of these states remained profoundly influenced by Marxism-Leninism; some of them, however, had formed largely non-communist
