標題: Titlebook: In Vitro Mutagenesis Protocols; Michael K. Trower Book 19961st edition Humana Press 1996 [打印本頁] 作者: Espionage 時間: 2025-3-21 16:47
書目名稱In Vitro Mutagenesis Protocols影響因子(影響力)
書目名稱In Vitro Mutagenesis Protocols影響因子(影響力)學科排名
書目名稱In Vitro Mutagenesis Protocols網(wǎng)絡公開度
書目名稱In Vitro Mutagenesis Protocols網(wǎng)絡公開度學科排名
書目名稱In Vitro Mutagenesis Protocols被引頻次
書目名稱In Vitro Mutagenesis Protocols被引頻次學科排名
書目名稱In Vitro Mutagenesis Protocols年度引用
書目名稱In Vitro Mutagenesis Protocols年度引用學科排名
書目名稱In Vitro Mutagenesis Protocols讀者反饋
書目名稱In Vitro Mutagenesis Protocols讀者反饋學科排名
作者: COW 時間: 2025-3-21 21:22 作者: probate 時間: 2025-3-22 02:25
Christian Hagemeier of the bond between them and their alleged vanguard party, the Volkskammer, the Volkspolizei, and the Volksarmee lost their power to the . and for a few brief weeks in October–November 1989, the East German people were drunk with the feeling of their own power and freedom.作者: 殘廢的火焰 時間: 2025-3-22 06:14
Susan J. Dale,Ian R. Felixharsh or unsafe conditions. In short, the labour of the ‘.’ (convicts) is ‘needed for degrading and particularly heavy work which no one, under socialism, would wish to perform’ (Solzhenitsyn, 1976: 560). Thus, it appears that slavery has been successfully reintroduced into Russia in the aftermath o作者: Indigence 時間: 2025-3-22 11:57
Scott A. Lesleyharsh or unsafe conditions. In short, the labour of the ‘.’ (convicts) is ‘needed for degrading and particularly heavy work which no one, under socialism, would wish to perform’ (Solzhenitsyn, 1976: 560). Thus, it appears that slavery has been successfully reintroduced into Russia in the aftermath o作者: JOG 時間: 2025-3-22 16:42
Derhsing Lai,Sidney Pestka in other than brief journalistic accounts; easily accessible English language literature on Soviet corruption, on the other hand, is relatively abundant. Before discussing top level corruption in these countries, however, the key concept of ‘nomenklatura’ has first to be introduced.作者: 公式 時間: 2025-3-22 18:50 作者: excursion 時間: 2025-3-22 23:22 作者: MAIM 時間: 2025-3-23 01:31 作者: 含糊 時間: 2025-3-23 06:15
Li Zhu,Ann E. Holtzslate goes forward as the ‘bloc of Communist and non-party candidates’, and since at least the Second World War it has never received less than 99 per cent support. A process of recall exists by which deputies can be replaced if an error appears to have been made in their selection (Art. 107). Usual作者: 拉開這車床 時間: 2025-3-23 12:24 作者: FECK 時間: 2025-3-23 16:21
Gerard J. A. Rouwendal,Emil J. H. Wolbert,Lute-Harm Zwiers,Jan Springer units of comparison becomes somewhat more arbitrary. Should we compare the communist states, for instance, with the major capitalist states, which the communist states have pledged themselves to overtake but which are still, by and large, at a more advanced stage of social and economic development?作者: 凌辱 時間: 2025-3-23 19:37 作者: 寡頭政治 時間: 2025-3-24 01:20
Robert J. Pogulis,Abbe N. Vallejo,Larry R. Peasethe only thing that has withered away is the . that the state should wither away). The communist states, on the contrary, have generally been large, powerful and authoritarian institutions, in which the rights and liberties of the citizen, at least in Western terms, have been systematically represse作者: Hirsutism 時間: 2025-3-24 03:44
Ashok Aiyar,Yan Xiang,Jonathan Leisbject to strict civilian control (as official pronouncements never tire in declaring) yet the country’s foremost civilian recently arranged to acquire, in his seventieth year, the military rank of marshal. The army uses the latest in nuclear and electronic technology yet the press routinely describe作者: overwrought 時間: 2025-3-24 07:11
Ivan Mikaelian,Alain Sergeantlker, 1980, p. 12). It is, of course, too soon to assess what the actual impact of state reforms will be, as opposed to their intention, not least since many have only just been implemented and it is reasonable to assume that more reforms are on the way. However, it is possible, by considering recen作者: plasma 時間: 2025-3-24 12:20 作者: Cloudburst 時間: 2025-3-24 17:24 作者: fixed-joint 時間: 2025-3-24 20:40
Site-Directed Mutagenesis Using Positive Antibiotic Selection,owing to such factors as incomplete in vitro DNA polymerization, primer displacement by the DNA polymerase used to synthesis the second strand, and in vivo host-directed mismatch repair mechanisms that favor the repair of the nonmethylated newly synthesized mutant strand (.). Several improvements ha作者: LIMN 時間: 2025-3-25 01:34
Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template,he PCR products. Alternatively, PCR amplification is used to create recombinant plasmid circles with discrete, cohesive single-stranded ends (.). This method also requires purification steps to remove excess primers. These circles, formed without the use of restriction enzyme digestion or ligation, 作者: Sinus-Node 時間: 2025-3-25 05:50
Site-Directed Mutagenesis In Vitro by Megaprimer PCR,uct (A-M1) is purified and used as one of the primers (hence the name “megaprimer”) in the second round of PCR along with the other flanking primer (B). The wild-type cloned gene is used as template in both PCR reactions. The final PCR product (A-M1-B) containing the mutation can be used in a variet作者: Indict 時間: 2025-3-25 10:54 作者: Hla461 時間: 2025-3-25 12:57
Li Zhuevy?del?Aguila?Marchena.?is Senior Professor and Chair of the?Department of Management Sciences at?the Pontificia Universidad Católica?del?Perú.?He has published extensively on Marx, political philosophy, and applied ethics..978-3-030-82896-7978-3-030-82894-3Series ISSN 2524-7123 Series E-ISSN 2524-7131 作者: MENT 時間: 2025-3-25 19:29
In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method,parental (wild-type) plasmid. The USE strategy employs two oligonucleotide primers: one primer (the mutagenic primer) produces the desired mutation, whereas the second primer (the selection primer) mutates a restriction site unique to the plasmid for the purpose of selection.作者: exceed 時間: 2025-3-25 20:41
Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template, given DNA sequence with high efficiency. I have used this procedure successfully many times and most recently to map protein interaction sites within the activation domain of the transcription factor E2F (.).作者: 他去就結束 時間: 2025-3-26 02:59
A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA,. Limitations of these methods include the availability of restriction sites for subcloning and the instability of large inserts in M13 vectors (.), the low fidelity of . polymerase, the cost of multiple primers in the PCR protocols, and the low mutant yields with the double-stranded plasmid method.作者: 泛濫 時間: 2025-3-26 04:51
Ordered Deletions Using Exonuclease III,.). ExoIII digests one strand of double-stranded DNA by removing nucleotides from 3′ ends if the end is blunt or has a 5′ protrusion. A 3′ protrusion of 4 bases or more is resistant to ExoIII digestion.作者: 無彈性 時間: 2025-3-26 11:38 作者: AMPLE 時間: 2025-3-26 12:45 作者: congenial 時間: 2025-3-26 18:54
PCR-Based Site-Directed Mutagenesis, mutagenesis reaction (.). By using this method, a series of site-directed mutations may be undertaken that only require a single primer for each desired change, and furthermore, no reiterative transformation steps are necessary.作者: 玉米 時間: 2025-3-27 00:37
Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template,cient than traditional methods for site-directed mutagenesis (.). However, because synthesis of two complementary primers is required for each mutation, the overlap extension is not the method of choice for extensive mutagenesis of a target sequence.作者: 簡略 時間: 2025-3-27 04:20 作者: 空洞 時間: 2025-3-27 08:03 作者: Rotator-Cuff 時間: 2025-3-27 12:07
Site-Directed Mutagenesis Using Overlap Extension PCR, other complementary regions, which form extensive secondary structures within single-stranded DNA, which often severely decrease the ability to extend annealed primers containing the mutation of interest.作者: 積極詞匯 時間: 2025-3-27 17:14
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates,cialized vectors, and convenient restriction sites or subcloning of the sequence of DNA to be mutated into a bacteriophage vector like M13 to produce and recover single-stranded DNA. These manipulations present major limitations to the routine use of these methods because they are time consuming and tedious.作者: 得罪 時間: 2025-3-27 20:49
Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach, (.,.) that has been improved to make it quicker and more convenient (.). Selection for the desired mutation takes place in vitro, allowing a high efficiency of mutagenesis to be achieved. Typically, an efficiency of greater than 85% mutants can be obtained with a range of types of mutation.作者: 神圣將軍 時間: 2025-3-28 00:49
Double-Stranded DNA Site-Directed Mutagenesis,and their interactions. Several protocols have been successfully employed to generate such mutants. Here I describe a “l(fā)inker-scanning” method that I have used to systematically mutate the murine major histocompatibility complex (MHC) class II Eα gene promoter (., ref. .).作者: 努力趕上 時間: 2025-3-28 06:10
Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis,.). Several techniques have been employed to enhance the frequency of clones containing the desired mutation by selecting against the parental strand through biological means or by in vitro manipulations (.–.).作者: 沙漠 時間: 2025-3-28 08:31
In Vitro Mutagenesis Protocols978-1-59259-544-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 膽小懦夫 時間: 2025-3-28 12:07 作者: 大量殺死 時間: 2025-3-28 17:36
A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restrictios provide a series of overlapping templates that can be used to generate a composite sequence with a single sequencing primer; in gene and protein functional studies, nested deletions allow researchers to locate and define a variety of functional domains based on regions of deletions.作者: 切碎 時間: 2025-3-28 20:36 作者: Angiogenesis 時間: 2025-3-29 01:20
Site-Directed Mutagenesis Using Positive Antibiotic Selection, of a mismatched oligonucleotide to a DNA template followed by second-strand synthesis by a DNA polymerase. These techniques provide efficient means for incorporating and selecting for the desired mutation (.–.). Oligonucleotide hybridization techniques use single-stranded DNA, usually derived from 作者: Debark 時間: 2025-3-29 05:56 作者: glomeruli 時間: 2025-3-29 09:23
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates,ing vectors. Several methods for performing this technique have appeared in the literature (.–.). They generally require multiple enzymatic steps, specialized vectors, and convenient restriction sites or subcloning of the sequence of DNA to be mutated into a bacteriophage vector like M13 to produce 作者: MEEK 時間: 2025-3-29 12:44
Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template,ed researchers to identify regions necessary for the regulation of gene expression. Also, it was and still is instrumental to learn about the importance of functional domains or even single amino acids of proteins. Of the many useful methods available for site-directed mutagenesis, in this chapter I作者: 議程 時間: 2025-3-29 17:26 作者: 命令變成大炮 時間: 2025-3-29 19:42 作者: Malleable 時間: 2025-3-30 03:50 作者: Preamble 時間: 2025-3-30 06:58 作者: Facilities 時間: 2025-3-30 10:54
Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template,study of structure-function relationships or modification of restriction endonuclease restriction sites. Several novel polymerase chain reaction (PCR)-based methods of in vitro mutagenesis have been described (.–.). Site-specific mutations are created by introducing mismatches into the oligonucleoti作者: VEST 時間: 2025-3-30 14:46 作者: 無辜 時間: 2025-3-30 19:05 作者: 寬宏大量 時間: 2025-3-30 21:54 作者: 花束 時間: 2025-3-31 01:04 作者: Water-Brash 時間: 2025-3-31 08:53 作者: Nonthreatening 時間: 2025-3-31 11:02 作者: CLAM 時間: 2025-3-31 15:47 作者: interpose 時間: 2025-3-31 20:39
Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template,so been widely used as a quick and efficient alternative for introducing mutations into specific DNA sequences. Various general methods for site-directed mutagenesis using the PCR have been described recently (.–.). In the overlap extension method two pairs of primers are used to generate two DNA fr作者: 多嘴多舌 時間: 2025-3-31 23:03
Site-Directed Mutagenesis In Vitro by Megaprimer PCR,le. The method utilizes three oligonucleotide primers and two rounds of PCR performed on a DNA template containing the cloned gene to be mutated (.–.). The basic megaprimer procedure is shown schematically in ., where A and B represent the “flanking” primers that can map either within the cloned gen作者: 良心 時間: 2025-4-1 02:37
Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids, present in the oligonucleotides used to prime the polymerization reactions will be incorporated in the resulting PCR products. Thus, a specific mutation can be introduced by designing mutagenic PCR primers. This principle has been used to introduce mutations into the DNA of bacterial plasmids (.–.)作者: addition 時間: 2025-4-1 09:35
, man a better life.’ Many people adapt themselves to the ruling spirit of corruption, follow the wrong way, do what everybody does. But there are always those who cannot be dishonest, ruthless and base, those who understand that moral decay threatens the existence of society and do not reconcile th作者: uveitis 時間: 2025-4-1 10:46 作者: Arable 時間: 2025-4-1 16:49
Li Zhubandonment of the political dimension of praxis in Marx‘s wo.This book investigates communism in Marx’s writings, incorporating a consideration of?.communist politicity.. The author outlines the arguments by which it is possible to sustain—from Marx—the idea that human emancipation against capital a作者: 攝取 時間: 2025-4-1 19:47 作者: overshadow 時間: 2025-4-1 23:33
Christian Hagemeieregin when . shouting ‘Wir wollen ’raus!’ scuffled with police outside Leipzig’s Nikolai Church nor even when others stormed the Dresden railroad station to try to board the trains carrying refugees to West Germany from the embassies in Prague and Warsaw. Although they may have precipitated the demis
