標(biāo)題: Titlebook: In Vitro Mutagenesis; Methods and Protocol Andrew Reeves Book 2017 Springer Science+Business Media New York 2017 gene and genome editing.fa [打印本頁(yè)] 作者: 服裝 時(shí)間: 2025-3-21 17:20
書(shū)目名稱(chēng)In Vitro Mutagenesis影響因子(影響力)
作者: 陰謀 時(shí)間: 2025-3-21 22:10
In Vitro Mutagenesis978-1-4939-6472-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: NAG 時(shí)間: 2025-3-22 01:36 作者: ACME 時(shí)間: 2025-3-22 06:33
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/463027.jpg作者: bisphosphonate 時(shí)間: 2025-3-22 09:41 作者: exigent 時(shí)間: 2025-3-22 16:30
All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells transient puromycin selection, to validate the mutation efficiency by Surveyor nuclease assay, and to perform off-target analyses. We show that our protocol enables highly efficient multiplex genome engineering even in hard-to-transfect HepG2 cells.作者: Eructation 時(shí)間: 2025-3-22 18:10 作者: lavish 時(shí)間: 2025-3-22 22:06
In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Varig predictions from multiple databases can facilitate the process. In this chapter, we describe the general steps of obtaining comprehensive predictions and annotations for large numbers of variants from dbNSFP, the first and probably the most widely used database of its kind.作者: Decibel 時(shí)間: 2025-3-23 04:24
In Silico Methods for Analyzing Mutagenesis Targetsamics are then demonstrated to evaluate the energy landscape, or potential of mean force, along the chosen reaction coordinate. The potential of mean force identifies variations of the predominant structures among mutants that may affect function.作者: 變白 時(shí)間: 2025-3-23 07:27 作者: 壓迫 時(shí)間: 2025-3-23 12:50 作者: Apogee 時(shí)間: 2025-3-23 14:31 作者: cluster 時(shí)間: 2025-3-23 22:03
Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Sne the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.作者: 懲罰 時(shí)間: 2025-3-24 00:24
Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Riceic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by . transformation.作者: 殺菌劑 時(shí)間: 2025-3-24 05:22
Generation of Stable Knockout Mammalian Cells by TALEN-Mediated Locus-Specific Gene Editingnd generation of TALENs using serial and hierarchical digestion and ligation steps, and using the synthesized TALEN pairs to achieve locus-specific targeted gene editing in mammalian cell lines using a modified clonal selection strategy in an easy and cost-efficient manner.作者: organism 時(shí)間: 2025-3-24 10:23
Use of Group II Intron Technology for Targeted Mutagenesis in ,utants in the obligate, intracellular bacterial pathogen .. The methods employed for intron targeting, mutant selection, and mutant verification will be outlined including available selection markers, gene targeting strategies, and potential pitfalls.作者: 戰(zhàn)勝 時(shí)間: 2025-3-24 12:44
Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned TechnCs injection (ICAHCI), which provides a new route to obtain genetically modified mice. In this chapter, we describe the procedures for AG-haESCs culturing, enrichment of haploid cells by FACS, genomic manipulation in DKO-AG-haESCs by CRISPR/Cas9 and generation of live SC mice with gene-modified DKO-AG-haESCs.作者: 意外的成功 時(shí)間: 2025-3-24 15:42
Mutagenesis and Genome Engineering of Epstein–Barr Virus in Cultured Human Cells by CRISPR/Cas9artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells.作者: 善變 時(shí)間: 2025-3-24 20:04
Development of CRISPR/Cas9 for Efficient Genome Editing in ,ntation. The technical details of the strategy, including CRISPR plasmid construction, target construct generation, parasite transfection and positive clone identification are also provided. These methods are easy to customize to any gene of interest (GOI) and will greatly accelerate studies on this important pathogen.作者: Expiration 時(shí)間: 2025-3-25 00:03 作者: Hyperplasia 時(shí)間: 2025-3-25 07:03 作者: 他一致 時(shí)間: 2025-3-25 08:08 作者: 使饑餓 時(shí)間: 2025-3-25 14:45
more than 50,000 trained and certified individuals worldwid.Measurement in human services means one thing: how well the effort serves clients. But the data doesn’t exist in a vacuum and must be communicated clearly between provider and client, provider and management, and across systems. During the作者: ADORE 時(shí)間: 2025-3-25 17:12
Ciaran M. Lee,Haibao Zhu,Timothy H. Davis,Harshahardhan Deshmukh,Gang Baoearly between provider and client, provider and management, and across systems. During the past decade, innovative communimetric measures have helped more than 50,000 professionals worldwide in health care, justice, and business settings deliver findings that enhance communication on all sides. Now,作者: 祖?zhèn)?nbsp; 時(shí)間: 2025-3-25 21:43 作者: 無(wú)能力 時(shí)間: 2025-3-26 03:56
Rongfang Xu,Pengcheng Wei,Jianbo Yangearly between provider and client, provider and management, and across systems. During the past decade, innovative communimetric measures have helped more than 50,000 professionals worldwide in health care, justice, and business settings deliver findings that enhance communication on all sides. Now,作者: 改良 時(shí)間: 2025-3-26 06:23
Tetsushi Sakuma,Takuya Sakamoto,Takashi Yamamotoearly between provider and client, provider and management, and across systems. During the past decade, innovative communimetric measures have helped more than 50,000 professionals worldwide in health care, justice, and business settings deliver findings that enhance communication on all sides. Now,作者: climax 時(shí)間: 2025-3-26 08:44 作者: profligate 時(shí)間: 2025-3-26 13:59 作者: 方便 時(shí)間: 2025-3-26 17:06
Zhenyu He,Kehkooi Keehe foreign policy crisis of the Ruhr occupation and the internal crisis of rampant inflation. The Cuno government’s decision to resort to passive resistance resulted in a ‘patriotic strike’, in the sabotage of railways, the cutting of electric cables, mass arrests by the occupying authorities, and o作者: Ardent 時(shí)間: 2025-3-26 21:37
Charlotte E. Key,Derek J. Fisherhe foreign policy crisis of the Ruhr occupation and the internal crisis of rampant inflation. The Cuno government’s decision to resort to passive resistance resulted in a ‘patriotic strike’, in the sabotage of railways, the cutting of electric cables, mass arrests by the occupying authorities, and o作者: Accord 時(shí)間: 2025-3-27 03:02 作者: Lacerate 時(shí)間: 2025-3-27 07:02
Brian A. Dow,Esha Sehanobish,Victor L. Davidsonea have been published, a comprehensive booklength study has not appeared thus far. The present volume is an initial step in that direction. It is, in the main, a political survey which has taken account of social and economic factors only when the par- ticular focus of the book demanded it. Since m作者: mutineer 時(shí)間: 2025-3-27 09:37 作者: 獨(dú)裁政府 時(shí)間: 2025-3-27 14:31
Troy C. Messina the Far East that today is called South-east Asia, they had to come to terms with varied and widespread sentiments and movements of nationalism that had already taken root there over a number of years. Both the concept of a distinctive South-east Asian region, comprising Thailand, Malaysia, Singapo作者: 瑣碎 時(shí)間: 2025-3-27 19:21
Nurit Haspel,Filip Jagodzinskiaw and Soviet Society, the Communist regime is founded to a large degree on the economic dependence of all citizens on the State, as an universal monopolist and a single employer. It is impossible to support such a regime by means of coercion only. Communism tries therefore to impress people with it作者: 開(kāi)始發(fā)作 時(shí)間: 2025-3-27 23:43
Nengding Wang,Egon A. Ozer, man a better life.’ Many people adapt themselves to the ruling spirit of corruption, follow the wrong way, do what everybody does. But there are always those who cannot be dishonest, ruthless and base, those who understand that moral decay threatens the existence of society and do not reconcile th作者: bibliophile 時(shí)間: 2025-3-28 03:26
Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Secision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and h作者: 不規(guī)則的跳動(dòng) 時(shí)間: 2025-3-28 08:21 作者: synovial-joint 時(shí)間: 2025-3-28 14:21
Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Riceg) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this ch作者: RODE 時(shí)間: 2025-3-28 16:23 作者: 都相信我的話(huà) 時(shí)間: 2025-3-28 21:13
CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium (hPSCs). In addition to facilitating hPSC-based disease studies, the application of genome engineering in hPSCs has also opened up new avenues for cell replacement therapy. To improve consistency and reproducibility of hPSC-based studies, and to meet the safety and regulatory requirements for clini作者: RAFF 時(shí)間: 2025-3-29 02:59
Development of CRISPR/Cas9 for Efficient Genome Editing in ,ears, the RNA-guided Cas9 nucleases derived from the prokaryotic type 2 CRISPR (clustered regularly interspaced short palindromic repeats) systems have drastically improved our ability to engineer the genomes of a variety of organisms including .. In this chapter, we describe detailed protocols for 作者: Forsake 時(shí)間: 2025-3-29 05:14
Generation of Stable Knockout Mammalian Cells by TALEN-Mediated Locus-Specific Gene Editingely effective and specific knockout strategy in both cultured cells and animal models. The current chapter describes a protocol for the construction and generation of TALENs using serial and hierarchical digestion and ligation steps, and using the synthesized TALEN pairs to achieve locus-specific ta作者: 態(tài)學(xué) 時(shí)間: 2025-3-29 10:09
Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned Technle pronucleus from zygotes. These cells, termed AG-haESCs, can be used in place of sperm to produce the so-called semi-cloned (SC) mice. Importantly, AG-haESCs carrying .-DMR and .-DMR knockouts (DKO-AG-haESCs) can efficiently and stably support the generation of SC mice via intracytoplasmic AG-haES作者: 彎腰 時(shí)間: 2025-3-29 14:24
Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genesve tools for genome editing, targeted mutagenesis and gene therapy applications. Herein, we present strategies where homing endonuclease open reading frames (HEases ORFs) are interrupted with group II intron sequences. The ultimate goal is to achieve in vivo expression of HEases that can be regulate作者: 組成 時(shí)間: 2025-3-29 19:25
Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells invented to fulfill these purposes, including zinc finger nucleases, TALEN, and CRISPR/Cas9. Although all of these systems can target DNA sequence with high efficiency, they also exert off-target effects and genotoxicity. The off-target effects might not hinder their usage in animal models because 作者: 粘土 時(shí)間: 2025-3-29 22:55
Use of Group II Intron Technology for Targeted Mutagenesis in ,trategies for bacterial pathogens. This chapter will discuss the application of a targeted, intron-based insertional mutagenesis method for creating mutants in the obligate, intracellular bacterial pathogen .. The methods employed for intron targeting, mutant selection, and mutant verification will 作者: surmount 時(shí)間: 2025-3-30 01:42
In Silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein–Cofactor and Proteinrotein under study. It is also very useful to have structures of multiple related proteins in order to determine whether or not particular amino acid residues are conserved in the structures either in the active site of an enzyme at the surface of a protein or at a putative protein–protein interface作者: eulogize 時(shí)間: 2025-3-30 07:25 作者: 熔巖 時(shí)間: 2025-3-30 08:47 作者: 注視 時(shí)間: 2025-3-30 14:27
Methods for Detecting Critical Residues in Proteinsdomain motions, and highly conserved residues on functional interfaces, which play a role in binding and interaction with other proteins. Detecting these regions facilitates the analysis and simulation of protein rigidity and conformational changes, and aids in characterizing protein–protein binding作者: Gorilla 時(shí)間: 2025-3-30 18:59
A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identifichroughput next generation sequencing to screen for changes in the composition of a pool of transposon mutants after exposure to selective conditions. One commonly used process for generating transposon insertion sequencing libraries is called INSeq that works with mutant pools produced using a modif作者: 羞辱 時(shí)間: 2025-3-30 23:46 作者: 槍支 時(shí)間: 2025-3-31 03:49 作者: FUME 時(shí)間: 2025-3-31 05:40 作者: 怕失去錢(qián) 時(shí)間: 2025-3-31 11:06
y to translate findings into practical action...This must-have volume offers readers the tools for understanding—and applying—this cutting-edge innovation by providing:.....The theoretical base for communimetri978-0-387-92821-0978-0-387-92822-7作者: Intellectual 時(shí)間: 2025-3-31 13:40 作者: 符合國(guó)情 時(shí)間: 2025-3-31 21:00
Bang Shen,Kevin Brown,Shaojun Long,L. David Sibleyy to translate findings into practical action...This must-have volume offers readers the tools for understanding—and applying—this cutting-edge innovation by providing:.....The theoretical base for communimetri978-0-387-92821-0978-0-387-92822-7作者: 尊嚴(yán) 時(shí)間: 2025-4-1 00:23
1064-3745 nd cutting-edge,?.In Vitro Mutagenesis: Methods and Protocols.?aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to th978-1-4939-8211-0978-1-4939-6472-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: tenosynovitis 時(shí)間: 2025-4-1 03:05 作者: 討厭 時(shí)間: 2025-4-1 06:56
