作者: 溺愛(ài) 時(shí)間: 2025-3-21 21:13
Boye Schnack Nielsen,Julia JonesIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts作者: 細(xì)頸瓶 時(shí)間: 2025-3-22 02:59
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462991.jpg作者: Mindfulness 時(shí)間: 2025-3-22 06:13 作者: 網(wǎng)絡(luò)添麻煩 時(shí)間: 2025-3-22 12:20 作者: choleretic 時(shí)間: 2025-3-22 14:56
RNAscope? Multiplex Detection in Zebrafishization studies. Presented here is a two-day protocol for robust multiplex detection of up to three different RNAs in zebrafish whole-mount embryos using the RNAscope procedure. Application of the protocol offers the simultaneous detection of multiple RNAs with a high signal-to-noise ratio in an intact embryo.作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-22 17:10
Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridizationue to masking of the specific signal across multiple wavelengths. Tissue sample variation can also affect levels of AF. In this chapter we share a method for overcoming the issues caused by sample variation and AF using HALO software on RNAscope in situ hybridization images.作者: COM 時(shí)間: 2025-3-22 22:50
Guidelines for the Optimization and Validation of In Situ Hybridization, appropriate controls, and protocol optimization as well as a central core message of understanding the target, understanding the ISH technique, and using the most appropriate controlling mechanisms to enable reproducible and trustworthy data to be obtained.作者: 模范 時(shí)間: 2025-3-23 01:56
Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope? in Cultured Cplexed and robust method of detecting low-abundance RNAs, with improved signal-to-noise ratio using RNAscope-based RNA-FISH which utilizes a series of amplification steps. We have validated this protocol for investigating the regulation of low-abundance lncRNAs, which would be ideal for in vitro screening in 96-well plates.作者: superfluous 時(shí)間: 2025-3-23 07:46 作者: Sputum 時(shí)間: 2025-3-23 13:45 作者: alliance 時(shí)間: 2025-3-23 17:54
Visualizing Genome Reorganization Using 3D DNA FISH in the reconstructed 3D images. In this way 3D DNA FISH provides a powerful tool to interrogate how the organization of specific genomic loci changes in response to stimuli. This chapter describes protocols which have allowed us to produce consistent data in cultured cells and paraffin-embedded tissue sections.作者: 保守黨 時(shí)間: 2025-3-23 18:36
Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Withinitation with subcellular resolution in an anatomical context and (2) dHCR imaging for digital mRNA absolute quantitation with single-molecule resolution in an anatomical context. Here, we provide protocols for qHCR and dHCR imaging in whole-mount zebrafish, chicken, and mouse embryos.作者: glisten 時(shí)間: 2025-3-24 01:55
Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope?ruses in formalin-fixed paraffin-embedded tissue sections from cassava. The dual-color protocol described can be used as reference for virus/host interaction studies including the visualization of virus nucleic acids and plant endogenous mRNAs. RNAscope provides a specificity and sensitivity of target detection that otherwise cannot be reached.作者: blackout 時(shí)間: 2025-3-24 03:28
Book 2020Latest editionsearchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; method作者: BRINK 時(shí)間: 2025-3-24 08:20
1064-3745 tion advice from the experts.This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multi作者: 流行 時(shí)間: 2025-3-24 14:42 作者: MITE 時(shí)間: 2025-3-24 18:38 作者: Credence 時(shí)間: 2025-3-24 22:36
Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboraty cancers. Multicolor FISH can be used to identify multiple targets, which can be simultaneously detected in individual cells using digital imaging microscopy. In an era of precision medicine there is a requirement to make a precise diagnosis and to have a molecular classification of the tumor that 作者: 閑聊 時(shí)間: 2025-3-25 01:15
Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probesbeen the most popular technique for chromosome identification in plants. Large insert genomic DNA clones, such as bacterial artificial chromosome (BAC) clones, and repetitive DNA sequences have been the most commonly used DNA probes for FISH. However, most of such traditional probes can only be used作者: Project 時(shí)間: 2025-3-25 04:56
Visualizing Genome Reorganization Using 3D DNA FISHfluorescence in situ hybridization (3D DNA FISH) labeled probes complementary to specific loci of interest are hybridized to the genome. The samples are then imaged using fluorescence microscopy, collecting z-stacks through the nuclei, and the relative positions of the hybridized probes are analyzed作者: 遵循的規(guī)范 時(shí)間: 2025-3-25 10:24 作者: 意見(jiàn)一致 時(shí)間: 2025-3-25 12:01 作者: 有惡臭 時(shí)間: 2025-3-25 17:03
Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR ial cells via flow cytometry. Third-generation in situ HCR (v3.0) provides automatic background suppression throughout the protocol, dramatically enhancing performance and ease of use. In situ HCR v3.0 supports analog mRNA relative quantitation via qHCR flow cytometry. Here, we provide protocols for作者: 規(guī)章 時(shí)間: 2025-3-25 21:54 作者: 極為憤怒 時(shí)間: 2025-3-26 02:56 作者: cyanosis 時(shí)間: 2025-3-26 04:53 作者: 浮雕寶石 時(shí)間: 2025-3-26 09:00 作者: neoplasm 時(shí)間: 2025-3-26 15:20
Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope?y technology for probe design and hybridization assay. The method has been extensively used as a basis for sensitive diagnostic assays in the medical field, while applications of this technique in plant sciences are still rare. Here, we describe a multiplex ISH protocol for detection of two plant vi作者: 火光在搖曳 時(shí)間: 2025-3-26 19:23 作者: 含水層 時(shí)間: 2025-3-26 22:43
y in organizations, the concept of communication climate is related to those of voice climate and psychological safety, both of which focus on the risk of speaking up and raising concerns at work. In this book the author ?addresses the scope for criticism, dissent and disagreement, but also for prai作者: 官僚統(tǒng)治 時(shí)間: 2025-3-27 03:23
or sorne realistic parallel cornputations. This chapter is devoted to the relation between communication complexity and the complexity rneasures of Boolean circuits. More precisely, we show which lower bounds can be derived on the complexity rneasures of Boolean circuits cornputing a Boolean functio作者: 賭博 時(shí)間: 2025-3-27 08:25
Julia Jones,William J. Howatit is a more powerful computing model than the Boolean circuit model. While the gates (processors) of VLSI circuits are as simple as the gates of Boolean circuits, the communication structure (the graph describing the connections between processors) of VLSI circuits may contain cycles. This requires作者: BULLY 時(shí)間: 2025-3-27 09:34
Cara Brodieofit organization that advocates for the plant-based movement. Currently, the certification is based in two existing labels, one of which identifies and guarantees that the products that contain it are vegan, while the other does the same for vegetarian products. However, the visual similarity betwe作者: 舔食 時(shí)間: 2025-3-27 16:08
tween countries. In this scenario, it is important to focus on world peace because world peace might influence the growth and prosperity of the entire global community. On the other hand, eye tracking is a relatively new technique for the study of visual attention and perception in poster design. Th作者: Conserve 時(shí)間: 2025-3-27 19:19
Sheila J. M. O’Connor,Kathryn R. Turner,Sharon L. Barrans emerged on the first day of the lockdown decreed in Spain in response to the COVID-19 pandemic. The movement was structured in several phases: the initial phase, .; the intermediate phase, .; and the final phase, .. This text describes how an entrepreneurial initiative that arose almost spontaneous作者: myalgia 時(shí)間: 2025-3-27 22:50
Guilherme T. Braz,Fan Yu,Lívia do Vale Martins,Jiming Jiangks to understand more deeply the importance of written narratives in the marketing field. In this sense, the objective of this article is to explore similarities and identify patterns, phases or stages, variables, indicators, and respective relationships (models) to help marketers to create storytel作者: Lice692 時(shí)間: 2025-3-28 04:59 作者: 裁決 時(shí)間: 2025-3-28 09:18
ment and then by a third part on language disorders. Thus the discussion of language disorders is the ultimate goal of this chapter. “Language-affecting disorders” would be a better, though more cumbersome term—it connotes the disturbance of the language function while avoiding the issue of primacy 作者: endocardium 時(shí)間: 2025-3-28 13:04 作者: hankering 時(shí)間: 2025-3-28 15:16 作者: 隼鷹 時(shí)間: 2025-3-28 21:48
Maayan Schwarzkopf,Harry M. T. Choi,Niles A. Pierceures has broadened the range of benefits and resources now employed for the auditorily impaired. Many of the advances in audiology are spinoffs from allied fields. From medicine, otologic surgeons developed middle ear reconstruction techniques that often restore hearing to normal acuity. Medical tre作者: CYT 時(shí)間: 2025-3-29 01:10
Maayan Schwarzkopf,Harry M. T. Choi,Niles A. Piercenformation and refers to “one or more individuals involved in an act of sending and receiving messages that are disturbed by noise, occur within a context, have some effect, and provide an opportunity for feedback” (De Vito, 1985, p. 3). This definition refers to the informative aspect of communicat作者: 人類的發(fā)源 時(shí)間: 2025-3-29 06:53
nformation and refers to “one or more individuals involved in an act of sending and receiving messages that are disturbed by noise, occur within a context, have some effect, and provide an opportunity for feedback” (De Vito, 1985, p. 3). This definition refers to the informative aspect of communicat作者: 不透氣 時(shí)間: 2025-3-29 09:27 作者: Project 時(shí)間: 2025-3-29 12:08 作者: 逢迎白雪 時(shí)間: 2025-3-29 17:09
Theresa Gross-Thebingons.Analysis based on 21000 surveys with senior communicatio.Exploring the implications of 10 years of data from more than 21,000 communication professionals across Europe, combined with case studies and interviews with senior communication directors from top European companies and organisations, th作者: Arresting 時(shí)間: 2025-3-29 21:40
Samar Sheat,Stephan Winter,Paolo Margariaons.Analysis based on 21000 surveys with senior communicatio.Exploring the implications of 10 years of data from more than 21,000 communication professionals across Europe, combined with case studies and interviews with senior communication directors from top European companies and organisations, th作者: Communal 時(shí)間: 2025-3-30 03:55
ons.Analysis based on 21000 surveys with senior communicatio.Exploring the implications of 10 years of data from more than 21,000 communication professionals across Europe, combined with case studies and interviews with senior communication directors from top European companies and organisations, th作者: Nonflammable 時(shí)間: 2025-3-30 04:46
Charles Pykeons.Analysis based on 21000 surveys with senior communicatio.Exploring the implications of 10 years of data from more than 21,000 communication professionals across Europe, combined with case studies and interviews with senior communication directors from top European companies and organisations, th作者: Comprise 時(shí)間: 2025-3-30 10:20 作者: obnoxious 時(shí)間: 2025-3-30 13:23
Theresa Gross-Thebing’s global and changing markets. The study behind this book, the European Communication Monitor, is known as the most comprehensive provider of reliable data in the communication field worldwide..978-3-319-48859-2978-3-319-48860-8作者: 反叛者 時(shí)間: 2025-3-30 19:15
Samar Sheat,Stephan Winter,Paolo Margaria’s global and changing markets. The study behind this book, the European Communication Monitor, is known as the most comprehensive provider of reliable data in the communication field worldwide..978-3-319-48859-2978-3-319-48860-8作者: LAVA 時(shí)間: 2025-3-31 00:02 作者: Ascribe 時(shí)間: 2025-3-31 04:45 作者: 揭穿真相 時(shí)間: 2025-3-31 05:16
1064-3745 eshooting and avoiding known pitfalls..Cutting-edge and thorough, .In Situ Hybridization Protocols, Fifth Edition. is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field.?.978-1-0716-0625-4978-1-0716-0623-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
