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標題: Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19992nd edition Humana Press 1999 DNA.ELISA.Filtration.Organelle.PCR.cell [打印本頁]

作者: 一再    時間: 2025-3-21 19:24
書目名稱Immunocytochemical Methods and Protocols影響因子(影響力)




書目名稱Immunocytochemical Methods and Protocols影響因子(影響力)學科排名




書目名稱Immunocytochemical Methods and Protocols網(wǎng)絡公開度




書目名稱Immunocytochemical Methods and Protocols網(wǎng)絡公開度學科排名




書目名稱Immunocytochemical Methods and Protocols被引頻次




書目名稱Immunocytochemical Methods and Protocols被引頻次學科排名




書目名稱Immunocytochemical Methods and Protocols年度引用




書目名稱Immunocytochemical Methods and Protocols年度引用學科排名




書目名稱Immunocytochemical Methods and Protocols讀者反饋




書目名稱Immunocytochemical Methods and Protocols讀者反饋學科排名





作者: 持續(xù)    時間: 2025-3-21 21:48
Overview of Cell Fixatives and Cell Membrane Permeantsroteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.
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Biotinylation of Antibodiespplied to the sample, and the detection is accomplished by using labeled avidin. Avidin with a variety of labels are available commercially, including fluorescent, enzyme, iodine, ferritin, or gold labels.
作者: 保全    時間: 2025-3-22 09:57
Book 19992nd editioncroscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization..
作者: 蟄伏    時間: 2025-3-22 15:12
1064-3745 , light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization..978-1-59259-213-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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作者: considerable    時間: 2025-3-23 07:24
Conjugation of Fluorochromes to Antibodiesed. The primary antigenic site is thus revealed. A major advantage of the indirect method is the enhanced sensitivity. In addition, a labeled secondary antibody can be used to locate any number of primary antibodies raised in the same animal species without the necessity of labeling each primary antibody.
作者: extract    時間: 2025-3-23 10:58
Overview of Antibody Use in Immunocytochemistry the antigenic sites (direct method) (.) (. Chapter 15). Later, the more sensitive and versatile indirect method was introduced (.) (. Chapters 16–18). In this method, the specific antibody, bound to the antigen, was detected with a secondary reagent, usually another antibody that had been tagged wi
作者: 粗鄙的人    時間: 2025-3-23 16:02
Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration charge, the surface distribution of polar side chains, and the size of the polypeptide, as well as the pH and temperature of the solution. Immunoglobulins precipitate at 40–50% ammonium sulfate saturation depending somewhat on the species and subclass (.). The desired saturation is brought about ei
作者: 安裝    時間: 2025-3-23 19:47

作者: Agnosia    時間: 2025-3-24 01:17
Purification of Antibodies Using Protein A-Sepharose and FPLCfor isolating immune complexes or immunoglobulins from a crude solution (.,.). Protein A has also been useful for separating Fc fragments from Fab fragments after proteolytic di gestion. The advantages of protein A are mainly its stability and specificity. Protein A is stable over a wide pH range (p
作者: Palate    時間: 2025-3-24 03:57

作者: ULCER    時間: 2025-3-24 10:12
Processing of Cytological Specimens a dilute medium are best suited for the preparation of cytospins through cytocentrifugation. Cell suspensions that exist in a high-viscosity medium, are best suited to be tested as swab preparations (.). The constant among these preparations is that the whole cell is present on the slide surface. F
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作者: ambivalence    時間: 2025-3-24 18:46
Processing of Tissue Specimensmple immersion fixation. It should be mentioned, though, that depositing large organs like whole brains in buckets of fixative may provide fine cellular detail but will probably result in the loss of some labile brain proteins desired for study (.). The individual who actually obtains the specimen i
作者: 等待    時間: 2025-3-24 20:20
Direct Immunofluorescent Labeling of Cellsfluorescence is weak since only one labeled primary antibody binds to each antigen. The indirect labeling technique adds one more incubation and wash step, but it has the advantage of amplifying the fluorescent signal because several fluorescently labeled secondary antibodies can bind to each primar
作者: 有惡臭    時間: 2025-3-25 00:03
Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cellshighly useful diagnostic tools in clinical medicine. The identification of surface antigenic molecules using antibodies can be performed by several methods, including immunofluorescence microscopy, enzyme and discrete marker microscopy, enzyme and radioactive labeling of mass cultures, and flow cyto
作者: 牽連    時間: 2025-3-25 04:11
hor to convey his personal enthusiasm and love for his subject. These volumes on classical physics,finally available in English, complement Greiner‘s texts on quantum physics, most of which have been available to English-speaking audiences for some time. The complete set of books will thus provide a
作者: 提名    時間: 2025-3-25 10:12
oximation isotropic and homogeneous – characterized by a . frequency spectrum with a temperature of about .K. This radiation is a messenger of a primordial era in which the Universe was made up mainly of dissociated charged particles and radiation, in thermal equilibrium with each other. This balanc
作者: 羽飾    時間: 2025-3-25 14:05
Su-Yau Mao,Lorette C. Javois,Ute M. Kentoximation isotropic and homogeneous – characterized by a . frequency spectrum with a temperature of about .K. This radiation is a messenger of a primordial era in which the Universe was made up mainly of dissociated charged particles and radiation, in thermal equilibrium with each other. This balanc
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作者: Congregate    時間: 2025-3-26 14:14
Gary L. Bratthauermagnetictheory, allthematerialcanbereasonablycoveredintwosemesters. Neophytes should probable spenda semester on the ?rst four or ?ve chapters as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave
作者: Priapism    時間: 2025-3-26 20:05
Gary L. Bratthauermagnetictheory, allthematerialcanbereasonablycoveredintwosemesters. Neophytes should probable spenda semester on the ?rst four or ?ve chapters as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave
作者: abject    時間: 2025-3-26 21:14
Laszlo Krenacs,Tibor Krenacs,Mark Raffeldmagnetictheory, allthematerialcanbereasonablycoveredintwosemesters. Neophytes should probable spenda semester on the ?rst four or ?ve chapters as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave
作者: wall-stress    時間: 2025-3-27 04:47
J. Michael Mullinsmagnetictheory, allthematerialcanbereasonablycoveredintwosemesters. Neophytes should probable spenda semester on the ?rst four or ?ve chapters as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave
作者: crumble    時間: 2025-3-27 08:22
Lorette C. Javoismagnetictheory, allthematerialcanbereasonablycoveredintwosemesters. Neophytes should probable spenda semester on the ?rst four or ?ve chapters as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave
作者: 交響樂    時間: 2025-3-27 10:39

作者: STIT    時間: 2025-3-27 16:54
Su-Yau Maors as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave978-90-481-6707-4978-1-4020-2700-0Series ISSN 0168-1222 Series E-ISSN 2365-6425
作者: Rustproof    時間: 2025-3-27 21:10
Su-Yau Maors as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave978-90-481-6707-4978-1-4020-2700-0Series ISSN 0168-1222 Series E-ISSN 2365-6425
作者: 親密    時間: 2025-3-27 22:08
rs as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave978-90-481-6707-4978-1-4020-2700-0Series ISSN 0168-1222 Series E-ISSN 2365-6425
作者: 案發(fā)地點    時間: 2025-3-28 03:30
Melissa A. Melanrs as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave978-90-481-6707-4978-1-4020-2700-0Series ISSN 0168-1222 Series E-ISSN 2365-6425
作者: 防水    時間: 2025-3-28 06:36
Gary L. Bratthauerrs as well as, depending on their mathematical background, the Appendices B to F. For a shorter or more elementary course, the material on spherical waves, wave978-90-481-6707-4978-1-4020-2700-0Series ISSN 0168-1222 Series E-ISSN 2365-6425
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作者: 顯赫的人    時間: 2025-3-29 02:09
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462124.jpg
作者: 婚姻生活    時間: 2025-3-29 04:19

作者: 多余    時間: 2025-3-29 10:44
ill analyze are the ., which form a particular complete set of solutions of Maxwell’s equations in ., or in ., that is to say, in the absence of charged sources throughout space .for all .. The phenomenological relevance of these solutions is self-evident: The major part of the energy provided by th
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Ute M. Kentlectromagnetic waves. In this chapter we address another fundamental problem of classical electrodynamics: the determination of the electromagnetic field generated by an arbitrary distribution of moving charges, which amounts to solve Maxwell’s equations in the presence of a generic four-current ..
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作者: 憤世嫉俗者    時間: 2025-3-30 14:05

作者: 無政府主義者    時間: 2025-3-30 18:02
d astronomer (1564-1642) This book is a second edition of “Classical Electromagnetic Theory” which derived from a set of lecture notes compiled over a number of years of teaching elect- magnetic theory to fourth year physics and electrical engineering students. These students had a previous exposure
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作者: ARM    時間: 2025-3-31 06:46
Gary L. Bratthauerer (1564-1642) This book is a second edition of “Classical Electromagnetic Theory” which derived from a set of lecture notes compiled over a number of years of teaching elect- magnetic theory to fourth year physics and electrical engineering students. These students had a previous exposure to electr
作者: Anticoagulant    時間: 2025-3-31 10:51
Gary L. Bratthauerd astronomer (1564-1642) This book is a second edition of “Classical Electromagnetic Theory” which derived from a set of lecture notes compiled over a number of years of teaching elect- magnetic theory to fourth year physics and electrical engineering students. These students had a previous exposure




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