作者: OTHER 時(shí)間: 2025-3-21 21:44 作者: crescendo 時(shí)間: 2025-3-22 01:08
Systems-Level Immune Monitoring by Mass Cytometry immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unpr作者: entail 時(shí)間: 2025-3-22 05:05
Immune Monitoring of Cancer Patients by Multi-color Flow Cytometryo these novel treatments. Multicolor flow cytometry is a powerful tool that enables tumor immunologists to characterize the immune system of patients before and in response to immunotherapy. We present here a protocol for purifying human peripheral blood mononuclear cells and staining them with a se作者: Dysplasia 時(shí)間: 2025-3-22 11:31
High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometryand other diseases. So far, methodologies for recovery of HLA class I and II peptides for subsequent mass spectrometry-based analysis have been a major limitation. In this chapter we describe a detailed protocol for a high-throughput, reproducible, and sensitive immunoaffinity-purification of HLA-I 作者: GEST 時(shí)間: 2025-3-22 15:40 作者: 我說不重要 時(shí)間: 2025-3-22 19:47
Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumorsom tumors is usually performed in two steps. The details of the procedure differ between different laboratories, but the general concept remains the same. In the first step, small fragments of a tumor are placed in culture medium containing one or more T cell stimulating growth factors. Here the mos作者: dainty 時(shí)間: 2025-3-22 22:05
Establishment of Two Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Toolottom of plastic flasks. Melanoma cells can be cultured with a considerable degree of success, and, depending on the further use of the cells obtained in the culture, methodologies have to be adjusted to obtain reliable results. Although there are many melanoma continuous cell lines, in vitro 2D and作者: 不可知論 時(shí)間: 2025-3-23 01:29 作者: Pruritus 時(shí)間: 2025-3-23 07:30
Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometryt these cells face major limitations, like the predetermination of the given HLA type. The herein described protocol provides a means of overcoming many of the obstacles associated with the use of multimers and other common approaches in this field. In order to be able to detect rare cells which are作者: Ergots 時(shí)間: 2025-3-23 10:52 作者: 壓碎 時(shí)間: 2025-3-23 14:55
Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assayc region of antibodies that at the same time bind specifically to cell surface antigens. ADCC stimulates the secretion of perforin, granzymes, and cytokines leading to lysis of the malignant cells. Natural killer (NK) cells express the CD16 receptor and can therefore be activated by ADCC to kill tum作者: 商議 時(shí)間: 2025-3-23 20:41 作者: 怒目而視 時(shí)間: 2025-3-23 22:55 作者: badinage 時(shí)間: 2025-3-24 03:14
Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-injection of Patim. The in vitro environment can lack the three-dimensional structure of the tumor, other cell types present within the tumor microenvironment, and important growth factors. Humanized mouse models allow researchers to study primary tumor cells in a more natural environment. With further development o作者: abysmal 時(shí)間: 2025-3-24 10:23 作者: 未完成 時(shí)間: 2025-3-24 11:09 作者: 不溶解 時(shí)間: 2025-3-24 16:49
ting original records into small groups of at least . elements and, therefore, preserving .-anonymity. Usually, in order to avoid information loss, when records are large, i.e., the number of attributes of the data set is large, this data set is split into smaller blocks of attributes and microaggre作者: 改進(jìn) 時(shí)間: 2025-3-24 19:10 作者: chapel 時(shí)間: 2025-3-24 23:33
Claudia Wickenhauser,Daniel Bethmann,Zipei Feng,Shawn M. Jensen,Carmen Ballesteros-Merino,Chiara Mas. In this context, crowdsourcing (or crowd computing) is becoming a common solution to provide answers to complex problems by automatically coordinating the potential of machines and human beings working together. Several challenges still separate crowdsourcing from its generalized acceptance by ind作者: FLASK 時(shí)間: 2025-3-25 03:57 作者: Hyperalgesia 時(shí)間: 2025-3-25 08:57 作者: Ischemia 時(shí)間: 2025-3-25 13:22
Fabio Marino,Chloe Chong,Justine Michaux,Michal Bassani-Sternbergly equipped classrooms in which new pedagogical approaches based on collaborative learning, project-based learning and personalized learning are being used, call for the need to rethink the classroom physical space. Despite of the existence of some new classroom physical spaces aiming to answer this作者: Friction 時(shí)間: 2025-3-25 16:33
Uta Sandy Tretbar,Michael Friedrich,Maria-Filothei Lazaridou,Barbara Seliger people. From the 1960’s, the rights of the children started to be recognized through the UN Convention on the Rights of the Child. Article 12 of the convention states that children should have the opportunity to express their views in matters that affect them, like urban planning. Our work started 作者: 貪心 時(shí)間: 2025-3-25 20:52
eas and wishes of the citizens at every stage of the public policy process. In addition to the changing approach, the crisis in the field of representative democracy has also made it common for citizens to participate in the public policy process through direct citizen participation tools. Although 作者: receptors 時(shí)間: 2025-3-26 01:03 作者: chiropractor 時(shí)間: 2025-3-26 04:58
Nataly Cruz Rodríguez,Johanna Lineros,Carol Stefany Rodríguez,Lina María Martínez,Josefa Antonia Rod, in a predictable way has pushed policymakers to collaborate with psychology methods and theories. Accordingly, in the recent policy design of public authorities, there is an increasing interest in the nudge approach, which is considered a less costly, more liberal, more citizen-focused alternative作者: 干涉 時(shí)間: 2025-3-26 12:20
Jeroen Melief,Stina Wickstr?m,Rolf Kiessling,Yago Pico de Coa?aheir services. The ultimate goal of a smart city is to make cities healthier and sustainable for its residents, remove bureaucratic barriers before access to public services, introduce innovative solutions to city-related problems, and consider citizens’ wishes and expectations. Smart cities that in作者: employor 時(shí)間: 2025-3-26 14:48 作者: 剛開始 時(shí)間: 2025-3-26 18:57 作者: 臭了生氣 時(shí)間: 2025-3-26 22:16
Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumorsctors. This second expansion step is usually around 2?weeks in length. Here will be described a REP that uses soluble anti-CD3 antibodies for TCR triggering, irradiated peripheral blood mononuclear cells (PBMC) as feeder cells, and IL-2 as the T cell growth factor. Furthermore, the described protoco作者: reception 時(shí)間: 2025-3-27 04:04
Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometryo be characterized. Furthermore, this method simultaneously detects CD4+ and CD8+ T cells that produce cytokines upon encounter with antigen and thereby provides a functional insight into the behavior of the responding T cells. In our case, we have investigated the pro- or anti-inflammatory cytokine作者: 土產(chǎn) 時(shí)間: 2025-3-27 09:05
Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro further be utilized to study the effects of immune checkpoint inhibitors in vitro and ex vivo. We focus on the differences between canonical NK cells and the newly identified subset of NK cells termed “adaptive NK cells.” These cells are induced by cytomegalovirus (CMV) in CMV-seropositive individu作者: 懸崖 時(shí)間: 2025-3-27 12:24 作者: FUME 時(shí)間: 2025-3-27 15:25
Shi Yong Neo,Aine O’Reilly,Yago Pico de Coa?agies, alongside new policies for smarter, proactive citizens – making them a valuable resource for researchers and policymakers alike..978-3-319-87052-6978-3-319-61322-2Series ISSN 2190-3018 Series E-ISSN 2190-3026 作者: 是他笨 時(shí)間: 2025-3-27 17:57
Alexander Martens,Graham Pawelec,Christopher Shipp作者: 出沒 時(shí)間: 2025-3-28 00:17 作者: modifier 時(shí)間: 2025-3-28 04:47 作者: 沙發(fā) 時(shí)間: 2025-3-28 07:11 作者: GRE 時(shí)間: 2025-3-28 11:41 作者: 反叛者 時(shí)間: 2025-3-28 18:06
called . (.). With ., the problem of .-anonymity loss is mitigated by mixing all the values in the original microdata file into a single non-attributed data set using a set of simple pre-processing steps and then, microaggregating all the mixed values together. Our experiments show that, using real 作者: 畏縮 時(shí)間: 2025-3-28 22:42 作者: Coronary 時(shí)間: 2025-3-29 02:40
Claudia Wickenhauser,Daniel Bethmann,Zipei Feng,Shawn M. Jensen,Carmen Ballesteros-Merino,Chiara Maspossible obstacles to be removed. In this paper, we discuss some of these issues, provide solutions to improve the quality in systems based on the use of crowdsourcing and present a real industrial problem where we use the crowd to leverage the work capacity of geographically distributed human being作者: Awning 時(shí)間: 2025-3-29 05:18 作者: 可卡 時(shí)間: 2025-3-29 10:08 作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-3-29 12:20
Uta Sandy Tretbar,Michael Friedrich,Maria-Filothei Lazaridou,Barbara Seligerpromoting awareness and critical thinking. Several user evaluations are performed during the development cycles, including expert evaluations, usability and field tests of the final prototype. Based on collected data and lessons learned, results are discussed in relation to participation and learnin作者: 袋鼠 時(shí)間: 2025-3-29 16:28
effective citizen participation. As a result, this chapter suggests increasing and supporting e-participation opportunities, designing scientific research to overcome the challenges in front of direct citizen participation, and increasing the examples in the literature regarding the design of the di作者: 雪崩 時(shí)間: 2025-3-29 22:18 作者: Ancillary 時(shí)間: 2025-3-30 03:16 作者: 軍火 時(shí)間: 2025-3-30 06:38
Jeroen Melief,Stina Wickstr?m,Rolf Kiessling,Yago Pico de Coa?arowdfunding, open government data, and free internet access - support for distance learning. This chapter concludes that it is essential for local communities to develop collective behavior to cope with a pandemic, and as technology users citizens roles are critical. Further developing digital healt作者: Gleason-score 時(shí)間: 2025-3-30 10:53 作者: 積習(xí)難改 時(shí)間: 2025-3-30 12:33
Book 2019, .Immune Checkpoint Blockade: Methods and Protocols. serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies..作者: mitten 時(shí)間: 2025-3-30 18:56 作者: 隨意 時(shí)間: 2025-3-30 21:03 作者: 僵硬 時(shí)間: 2025-3-31 03:33
Yago Pico de Coa?aIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 結(jié)束 時(shí)間: 2025-3-31 06:11
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462041.jpg作者: ASTER 時(shí)間: 2025-3-31 13:12
https://doi.org/10.1007/978-1-4939-8979-9Tumor immunology; Immunotherapy; Antitumoral immune responses; Functional assays; Cancer cell lines; Tran作者: 過份好問 時(shí)間: 2025-3-31 17:24
Double Immunohistochemistry and Digital Image Analysishan one target protein in the same tissue section. This chapter provides general guidelines for double IHC staining of formalin-fixed, paraffin-embedded tissue sections. Chromogenic substrates are chosen based on their excellent contrast and compatibility with the subsequent digital image analysis.
