標(biāo)題: Titlebook: Imaging Living Cells; Rosario Rizzuto,Cristina Fasolato Book 1999 Springer-Verlag Berlin Heidelberg 1999 Calcium.Calcium Dyes.Confocal Mic [打印本頁] 作者: 延展 時(shí)間: 2025-3-21 18:56
書目名稱Imaging Living Cells影響因子(影響力)
書目名稱Imaging Living Cells影響因子(影響力)學(xué)科排名
書目名稱Imaging Living Cells網(wǎng)絡(luò)公開度
書目名稱Imaging Living Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Imaging Living Cells被引頻次
書目名稱Imaging Living Cells被引頻次學(xué)科排名
書目名稱Imaging Living Cells年度引用
書目名稱Imaging Living Cells年度引用學(xué)科排名
書目名稱Imaging Living Cells讀者反饋
書目名稱Imaging Living Cells讀者反饋學(xué)科排名
作者: 火光在搖曳 時(shí)間: 2025-3-21 23:46 作者: Latency 時(shí)間: 2025-3-22 03:45 作者: HACK 時(shí)間: 2025-3-22 07:30
are given for every protocolImproved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together作者: Triglyceride 時(shí)間: 2025-3-22 10:30
Confocal Calcium Imaging of a number of individual cells. Under these circumstances, the only significant fluorescence is from the cell monolayer, and using a confocal microscope gives no significant improvement in performance.作者: 天賦 時(shí)間: 2025-3-22 16:35
Targeting, Expressing and Calibrating Recombinant Aequorin excellent signal to noise ratio, and are widely employed, for example, for the monitoring of gene expression in transfected cells or organisms, and the fluorescent proteins. Among the latter, green fluorescent protein (GFP) of ., which has recently attracted a huge interest, will be discussed in other sections of this book.作者: Fresco 時(shí)間: 2025-3-22 17:10 作者: Antioxidant 時(shí)間: 2025-3-23 00:43
Imaging Green Fluorescent Proteins in Mammalian Cellsiments, with many different applications that for reason of brevity we will not be able to discuss. In this chapter, we will essentially describe how to look at Green Fluorescent Protein (GFP) and optimize its fluorescence signal.作者: 漫步 時(shí)間: 2025-3-23 04:00 作者: ARBOR 時(shí)間: 2025-3-23 06:46 作者: Cerebrovascular 時(shí)間: 2025-3-23 11:16 作者: 嚴(yán)厲批評(píng) 時(shí)間: 2025-3-23 17:30 作者: JAMB 時(shí)間: 2025-3-23 21:55 作者: prediabetes 時(shí)間: 2025-3-24 01:46 作者: tattle 時(shí)間: 2025-3-24 03:07
Strategies for Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-Sensitivolled instrumentation for acquiring data from living tissue at fast rates has in turn made it possible to acquire ultra low light level data from these probes and to analyse either images or temporal changes in light emission, or both.作者: Cholecystokinin 時(shí)間: 2025-3-24 07:26
High Resolution 3-D Imaging of Living Cells by Image Restorationoach integrates sensitive CCD cameras, wide field epifluorescence microscopes and a computational method, image restoration, to obtain 3-D images with a lateral resolution of 100 nanometers, which is better than the resolution that can be achieved by either confocal fluorescence microscopy or conven作者: WAG 時(shí)間: 2025-3-24 11:27
Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes: General Principles ions were essential for the normal contraction of the frog heart. In 1957 Hodgkin and Keynes used .Ca. to trace the movements of the ion in the squid giant axon, and since that time many investigators have employed isotopes to examine Ca. fluxes in cells, tissues, or whole organs. These studies pro作者: fixed-joint 時(shí)間: 2025-3-24 17:17
Confocal Calcium Imagings of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca.].) use monolayers of cells in culture. After loading a calcium indicator dye into the cells, we place the cells on a fluorescence microscope and measure the signal from each作者: 省略 時(shí)間: 2025-3-24 22:40 作者: moribund 時(shí)間: 2025-3-25 03:10 作者: 慷慨援助 時(shí)間: 2025-3-25 03:55
Quantitative Calcium Imaging in Brain Slicesand enables to study visually-identified cell types. Since the majority of cells in a brain tissue slice preserve their structure and their synaptic contacts, this preparation is particularly useful to elucidate the properties of the synaptic interaction. Using Ca. imaging techniques, many intriguin作者: insurgent 時(shí)間: 2025-3-25 09:14
Imaging of Cytosolic Sodium and pH Using SBFI and BCECF, tight regulation of other key ions is vital to the survival and normal function of cells. In particular, a considerable amount of their energy (20–70%) is expended via the plasma membrane Na./K.ATPase to ensure that the cytosolic Na. concentration ([Na.].) is relatively low compared with that in t作者: embolus 時(shí)間: 2025-3-25 13:21
Common Methods for Measuring Apoptotic Cell Death by Flow Cytometryn and in the control of immune response.. In some cases, it consists in an ordered sequence of cellular events that start with the transcription of specific genomic sequences, synthesis of specific proteins, and activation of nucleases that cut DNA in the oligonucleosomal subunits. In other models t作者: Criteria 時(shí)間: 2025-3-25 19:20 作者: GIDDY 時(shí)間: 2025-3-25 20:07
Targeting, Expressing and Calibrating Recombinant Aequorinbeen responsible for the large expansion in the use of protein probes in cell biology. Two groups of reporter proteins are currently employed, derived from the wide variety of bioluminescent organisms: the chemiluminescent proteins (e.g. the different types of luciferases), which are endowed with an作者: 數(shù)量 時(shí)間: 2025-3-26 00:52
Imaging Ca2+ in Small Mammalian Cells with Recombinant Targeted Aequoriniving cells. Chief amongst these are: (i) zero toxicity; (ii) near-zero buffering of Ca.; (ii) low mobility; (iv) enormous dynamic range (0.1–100 μM); (v) ability to express the protein as a recombinant chimaera, and thus achieve targeting to subcellular domains where changes in [Ca.] would otherwis作者: Custodian 時(shí)間: 2025-3-26 06:09 作者: 謙卑 時(shí)間: 2025-3-26 11:34 作者: Maximizer 時(shí)間: 2025-3-26 13:20
The New Green Fluorescent Protein Mutants and their Applicationsology. Briefly, GFP has been successfully used (i) as a fluorescent gene reporter., which offers several advantages over the chemiluminescent enzyme luciferase, (ii) as a morphological probe of different intracellular compartments,. and (iii) as a marker allowing the detection in intact cells of sev作者: Bouquet 時(shí)間: 2025-3-26 20:12 作者: antiandrogen 時(shí)間: 2025-3-26 21:59 作者: 發(fā)炎 時(shí)間: 2025-3-27 03:44
isms of the mind, encompassing a wide spectrum of results and analyses. The book should appeal to scientists and graduate students in the fields of neuroscience, neurology, psychiatry, physiology, psychology, physics and philosophy. Its goals are the development of an empirical-analytical construct,作者: Sigmoidoscopy 時(shí)間: 2025-3-27 07:42 作者: Presbycusis 時(shí)間: 2025-3-27 11:45
Walter A. Carrington,Kevin E. Fogarty,Lawrence M. Lifshitz,Richard A. Tuftls for neuromodulation and for clinical neurorehabilitation purposes. They are particularly appealing in clinical contexts where physical movement is impaired, for instance following stroke. The intrinsic advantage of Brain-Computer Interfaces (BCIs) over alternate rehabilitation strategies is that 作者: 暫時(shí)別動(dòng) 時(shí)間: 2025-3-27 16:23
can facilitate motor recovery after severe stroke. However, improvements are heterogeneous across stroke patients. Thus, it is important to identify biomarkers that predict BCI training-related motor recovery and to find novel approaches to enhance training efficacy. Based on our most recent results作者: 清真寺 時(shí)間: 2025-3-27 21:47
Aldebaran M. Hofer,Wim J. J. M. Scheenenvement-related cortical potential (MRCP) as a subject imagines a dorsiflexion task and triggering an electrical stimulator to generate a single stimulus to the target nerve. This system has been tested on healthy subjects to demonstrate that an artificially generated signal (the peripheral afferent 作者: 多產(chǎn)子 時(shí)間: 2025-3-27 22:41
Stephen Bolsover conditions over the past decade. When compared to scalp electroencephalography (EEG), cortical recordings have demonstrated superior spatial resolution and consequently a greater potential for cognitive command output. Traditional cortical arrays require direct implantation into the brain via open 作者: ONYM 時(shí)間: 2025-3-28 03:44 作者: Crumple 時(shí)間: 2025-3-28 09:19
Adam Zweifach,Markus Hoth,Richard S. LewisresearchThe Annual BCI Research Awards are international prizes that recognize the top new projects in brain–computer interface (BCI) research.?This book contains concise descriptions of projects nominated for the 2020 BCI Research Award and interviews with nominees. Each article is authored by the 作者: Aprope 時(shí)間: 2025-3-28 12:17
Olga Garaschuk,Arthur Konnerthtouch to noxious stimuli. These are essential components for body protection as well as for restoring the sense of embodiment. Despite the considerable advances that have been made in designing suitable sensors and restoring tactile perceptions, pain perception dynamics and how to decode them using 作者: Flustered 時(shí)間: 2025-3-28 15:55
Alec W. M. Simpson,Ram V. Sharmaifficulty is a challenging task. Brain-computer interfaces would be suitable for such difficulty adaptations since they capture both conscious and subconscious aspects of workload, but have seen little use in rehabilitation. This chapter reviews previous work on passive brain–computer interfaces and作者: deface 時(shí)間: 2025-3-28 20:15
Ildo Nicoletti,Roberta Mannucci,Francesco Di Virgilioifficulty is a challenging task. Brain-computer interfaces would be suitable for such difficulty adaptations since they capture both conscious and subconscious aspects of workload, but have seen little use in rehabilitation. This chapter reviews previous work on passive brain–computer interfaces and作者: GIBE 時(shí)間: 2025-3-29 00:25 作者: 奇思怪想 時(shí)間: 2025-3-29 03:30
induced by musculoskeletal pain. The EEG activity patterns of participants with chronic pain (tennis elbow) were differentiated from those of healthy, age and sex matched controls during real-time movement performance. Our results showed a dominance of power in the alpha frequency range only that w作者: fixed-joint 時(shí)間: 2025-3-29 09:37 作者: exquisite 時(shí)間: 2025-3-29 13:31 作者: COUCH 時(shí)間: 2025-3-29 17:49 作者: 乳白光 時(shí)間: 2025-3-29 23:46
Marta Murgia,Rosario Rizzutolso a way to rehabilitate some motor or cognitive functions. Brain plasticity can help restore normal brain functions by inducing brain activity. In fact, voluntary event-related desynchronization (ERD) in upper alpha and beta electroencephalogram (EEG) activity bands is associated with different ne作者: 600 時(shí)間: 2025-3-30 02:44
Francesca De Giorgi,Rosario Rizzuto event-related potential (ERP) phase-locked to the event. However, it was unclear whether the more sophisticated face-evoked potentials can also be modulated by related mental tasks under the oddball paradigm. This study investigated ERP responses to image stimuli of objects, neutral faces, and emot作者: syncope 時(shí)間: 2025-3-30 07:08
Jim Haseloffmeans of communication and they have represented the target population for brain–computer interface (BCI) research in the last 15 years. Although different paradigms have been tested as well as different physiological signals have been used, to date no documented CLIS patient was able to control a B作者: arbiter 時(shí)間: 2025-3-30 10:19 作者: aerobic 時(shí)間: 2025-3-30 16:09
https://doi.org/10.1007/978-3-642-60003-6Calcium; Calcium Dyes; Confocal Microscopy; GFP; Path-Clamp Imaging; cells; cytoplasm; gene expression; prot作者: Range-Of-Motion 時(shí)間: 2025-3-30 19:46 作者: obscurity 時(shí)間: 2025-3-30 22:51
Rosario Rizzuto,Cristina FasolatoFirst manual describing novel labelling strategies * Easy to follow step-by-step instructions * Practical hints and experimental strategies are given for every protocol作者: Cantankerous 時(shí)間: 2025-3-31 01:06
Springer Lab Manualshttp://image.papertrans.cn/i/image/461666.jpg作者: RODE 時(shí)間: 2025-3-31 07:29
Imaging Whole Organs — Single cell [Ca2+]i measurements in the perfused liverovessels of a perfused lung,. omega neuron in a conscious cricket brain,. and pyramidal neurons in anaesthetized rat brain.. Additional insight into . [Ca.]. regulatory mechanisms have been uncovered in tissue preparations which maintain the functional unit of the intact organ such as, pyramidal neu作者: 震驚 時(shí)間: 2025-3-31 13:03
Imaging of Cytosolic Sodium and pH Using SBFI and BCECFrs and amino acids via co-transporters,. and to the rapid changes in membrane potential which propagate electrochemical signals in excitable cells.. These latter depolarisations ultimately lead to elevation of [Ca.]. in the presynaptic terminals, neuromuscular junctions and cell bodies via the openi作者: Perineum 時(shí)間: 2025-3-31 13:43
