標題: Titlebook: Imaging Gene Expression; Methods and Protocol Yaron Shav-Tal Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer [打印本頁] 作者: GERD847 時間: 2025-3-21 18:29
書目名稱Imaging Gene Expression影響因子(影響力)
書目名稱Imaging Gene Expression影響因子(影響力)學科排名
書目名稱Imaging Gene Expression網(wǎng)絡(luò)公開度
書目名稱Imaging Gene Expression網(wǎng)絡(luò)公開度學科排名
書目名稱Imaging Gene Expression被引頻次
書目名稱Imaging Gene Expression被引頻次學科排名
書目名稱Imaging Gene Expression年度引用
書目名稱Imaging Gene Expression年度引用學科排名
書目名稱Imaging Gene Expression讀者反饋
書目名稱Imaging Gene Expression讀者反饋學科排名
作者: antedate 時間: 2025-3-21 23:09 作者: Mosaic 時間: 2025-3-22 03:56
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Siresented here will allow the user to investigate mRNA transfer of most transcripts in any co-culture system. In addition, we present modifications that improve nanotube preservation during the smFISH procedure.作者: instructive 時間: 2025-3-22 07:58 作者: 有限 時間: 2025-3-22 11:47
Programmable Chromosome Painting with Oligopaintswith sequential FISH to label entire genomes with a single denaturation step. Here, we outline a protocol and considerations to scale the Oligopaint technology for fluorescent labeling of whole chromosomes.作者: MUTED 時間: 2025-3-22 13:08
Book 2019Latest editionhese updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, an作者: FLIC 時間: 2025-3-22 18:05 作者: insurrection 時間: 2025-3-22 23:51 作者: induct 時間: 2025-3-23 01:46 作者: N斯巴達人 時間: 2025-3-23 07:59 作者: 吊胃口 時間: 2025-3-23 10:14
Live-Cell Imaging of Long Noncoding RNAs Using Molecular Beaconsd on molecular beacons (MBs), which are a class of fluorogenic oligonucleotide-based probes with the capacity to convert RNA target hybridization into a measurable fluorescence signal. In this chapter, we describe the detailed protocol of using MBs to illuminate lncRNA transcripts at the single-molecule level in living cells.作者: 遵循的規(guī)范 時間: 2025-3-23 15:06
Visualization of Single mRNAs in Live Neuronse neurons. Then, GFP-labeled RNAs in live neurons can be detected by epifluorescence microscopy, and their moving pathways can be analyzed using single-particle tracking software. For these processes, we introduce protocols for neuron culture, transfection, imaging, and particle tracking methods.作者: KEGEL 時間: 2025-3-23 21:48
Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysisndividual mRNA particles. It colocalizes tracks and applies the colocalization information to quantify the number of intact transcripts and degradation intermediates. Based on the analysis of control data, the workflow further determines detection efficiencies and uses them to correct RNA particle numbers.作者: acrophobia 時間: 2025-3-24 00:32
Detection of mRNAs Anchored to the Nuclear Envelope During Export Inhibition in Living Cells examine their intracellular distribution and dynamics both in the nucleoplasm and at the nuclear periphery. We use this method to identify and count the number of static mRNPs anchored to the nuclear envelope under different conditions of mRNA export inhibition.作者: Conduit 時間: 2025-3-24 04:00 作者: Pruritus 時間: 2025-3-24 08:09 作者: 侵害 時間: 2025-3-24 12:08
Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP Systemogical question triggered the development of the MS2-MCP (MS2-Capsid Protein) system to tag mRNAs and image their life cycle using widefield fluorescence microscopy. The last two decades have evolved toward improving the qualitative and quantitative characteristics of the MS2-MCP system. Here, we pr作者: 慟哭 時間: 2025-3-24 17:20 作者: Ballad 時間: 2025-3-24 20:10 作者: 我不死扛 時間: 2025-3-25 01:17 作者: 震驚 時間: 2025-3-25 04:46
Lighting Up Gene Activation in Living , Embryosdeally suited to study the establishment of gene expression patterns during development. Recent improvements in RNA labeling technologies and confocal microscopy allow?for visualizing gene activation and quantifying transcriptional dynamics in living . embryos. Here we review the available tools for作者: Goblet-Cells 時間: 2025-3-25 10:47
Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysison have been identified, we still lack a basic understanding of the principles that regulate the spatiotemporal dynamics of mRNA turnover within single cells. To overcome this limitation, we have developed the TREAT biosensor, which allows for discrimination of intact reporter transcripts and stabil作者: hankering 時間: 2025-3-25 14:46
Imaging rRNA Methylation in Bacteria by MR-FISHuts. These bulk methods require the RNA from ~10. to 10. cells to be pooled to generate sufficient material for analysis. Here we describe a method—methylation-sensitive RNA in situ hybridization (MR-FISH)—that assays rRNA methylation in bacteria on a cell-by-cell basis, using methylation-sensitive 作者: BOAST 時間: 2025-3-25 18:50
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Sianotubes, which are long thin protrusions that are produced by numerous cell types and can connect cells that can be up to hundreds of microns apart. Potentially, mRNAs might also transfer via extracellular vesicles (EVs). Here we describe a method to detect transferred mRNA in cocultures of two dif作者: 先行 時間: 2025-3-25 23:37 作者: d-limonene 時間: 2025-3-26 01:26 作者: 維持 時間: 2025-3-26 06:26
Programmable Chromosome Painting with Oligopaintsst to conventional sources of probe, Oligopaints are computationally designed, synthesized on microarrays, and amplified by PCR. This approach allows for precise control over the sequences they target, which can range from a few kilobases to entire chromosomes with the same basic protocol. We have u作者: chemoprevention 時間: 2025-3-26 09:29 作者: 單片眼鏡 時間: 2025-3-26 13:22 作者: 完成才會征服 時間: 2025-3-26 18:53 作者: intuition 時間: 2025-3-26 22:00
Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analfluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.作者: 形容詞 時間: 2025-3-27 05:05
Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaggoing cell division. The few TFs that were observed to remain bound to chromosomes have been termed mitotic bookmarkers and were predicted to play important roles in reestablishing transcription after mitosis. Using live-cell imaging of endogenous TFs in mouse embryonic stem cells, we discovered tha作者: 激勵 時間: 2025-3-27 08:07 作者: Incompetent 時間: 2025-3-27 10:59
erapy, and chemotherapy leading to an overall survival (OS) of 70–80%, but survivors suffer from severe side effects. Based on gene expression, MB is divided in four different molecular subgroups—WNT, SHH, Group 3 (G3), and Group 4 (G4)—which differ in terms of clinics, prognosis, genetic alteration作者: paradigm 時間: 2025-3-27 16:37
the extensive invasion and diffusion of tumor cells anywhere within the host brain tissue, which makes almost impossible the complete surgical removal. Furthermore, although the blood-brain barrier (BBB) is typically disrupted at the core of GBM, it can be structurally conserved at the tumor periphe作者: Bureaucracy 時間: 2025-3-27 19:59
Maria Vera,Evelina Tutucci,Robert H. Singerthe extensive invasion and diffusion of tumor cells anywhere within the host brain tissue, which makes almost impossible the complete surgical removal. Furthermore, although the blood-brain barrier (BBB) is typically disrupted at the core of GBM, it can be structurally conserved at the tumor periphe作者: 饑荒 時間: 2025-3-27 22:31
Yachen Ying,Shiqi Mao,Christopher J. Krueger,Antony K. Chenlected through death certificates. Comparison between mortality rates (number of deaths/100000 inhabitants per year) of various countries in the periods 1951-1958 [1118] and 1967-1973 [124, 125] has shown a persistent geographic variability and a clear tendency to increase with time. The annual age-作者: 清醒 時間: 2025-3-28 04:39 作者: 預知 時間: 2025-3-28 07:22
Jae Youn Shim,Byung Hun Lee,Hye Yoon Parkst of the cases were published only after the advent of the Nissl stain for ganglion cells. Here our knowledge has been enriched particularly by Bielschowsky and Henneberg; Herxheimer; Pick and Bielschowsky; Schmincke (1909, 1910, 1914); Courville (1930); Christensen (1937), and above all by Foerste作者: TERRA 時間: 2025-3-28 12:05 作者: 我還要背著他 時間: 2025-3-28 17:55
Franka Voigt,Jan Eglinger,Jeffrey A. Chaocare management.Easy-to-read format.This concise book serves as a practical primer for the care of patients with primary brain tumors. ..?..Divided into two major sections, the first provides chapters with key information for each major brain tumor type.?This includes basic information about etiolog作者: gout109 時間: 2025-3-28 19:41 作者: Addictive 時間: 2025-3-29 00:00 作者: Embolic-Stroke 時間: 2025-3-29 06:57
Azra Lari,Farzin Farzam,Pierre Bensidoun,Marlene Oeffinger,Daniel Zenklusen,David Grunwald,Ben Montp used method of brain tumor classification is that of the World Health Organization (WHO), most recently revised in 2000 (.), which is based on microscopic examination of tissue by a pathologist. The WHO classification divides nervous system tumors into many nosological entities (Table 1) and assign作者: hematuria 時間: 2025-3-29 07:25 作者: achlorhydria 時間: 2025-3-29 14:08
croscopic and microscopic features and which up to the present were insufficiently defined. In the WHO classification (p. 18), however, terminology and subclassification appear to be very logical. This classification is based upon the predominant cell type but also includes mixed and anaplastic form作者: Desert 時間: 2025-3-29 15:43 作者: grieve 時間: 2025-3-29 21:42
Roopali Pradhan,Kundan Senguptacorrelations..Specifically focuses on art-based therapy (use.This book analyzes and discusses in detail art therapy, a specific tool used to sustain health in affective developments, rehabilitation, motor skills and cognitive functions.?Art therapy is based on the assumption that the process of maki作者: 罐里有戒指 時間: 2025-3-30 03:28
Kathrin S. Heinz,M. Cristina Cardosod. Mind is interpreted by the brain. AIcmaeon of Croton (5th Century B. c. ) The ground is shifting under the traditional approaches to problems in the philosophy of mind. Earlier doctrines concerning the independence of cognition from the brain now appear untenable. P. S. Churchland (20th Century A作者: Costume 時間: 2025-3-30 07:02
Sascha Conic,Dominique Desplancq,Alexia Ferrand,Nacho Molina,Etienne Weiss,László Torad. Mind is interpreted by the brain. AIcmaeon of Croton (5th Century B. c. ) The ground is shifting under the traditional approaches to problems in the philosophy of mind. Earlier doctrines concerning the independence of cognition from the brain now appear untenable. P. S. Churchland (20th Century A作者: 單調(diào)女 時間: 2025-3-30 11:37 作者: 玩笑 時間: 2025-3-30 15:17 作者: Bmd955 時間: 2025-3-30 16:41
Jorge Trojanowski,Anne Rademacher,Fabian Erdel,Karsten Rippe ofthe mind. Mind is interpreted by the brain. AIcmaeon of Croton (5th Century B. c. ) The ground is shifting under the traditional approaches to problems in the philosophy of mind. Earlier doctrines concerning the independence of cognition from the brain now appear untenable. P. S. Churchland (20th作者: 柔聲地說 時間: 2025-3-31 00:28
Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analfluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.作者: STYX 時間: 2025-3-31 02:20
https://doi.org/10.1007/978-1-4939-9674-2FISH; Drosophila; mRNAs; Endoplasmic Reticulum; ; RNA Pol II; CRISPR; gene expression; Biological Microscopy作者: Indict 時間: 2025-3-31 06:03 作者: 記憶法 時間: 2025-3-31 10:08
Imaging Gene Expression978-1-4939-9674-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Budget 時間: 2025-3-31 14:07
Yaron Shav-TalIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
