標(biāo)題: Titlebook: Hypoxia; Methods and Protocol Daniele M. Gilkes Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sprin [打印本頁] 作者: quick-relievers 時間: 2025-3-21 18:34
書目名稱Hypoxia影響因子(影響力)
書目名稱Hypoxia影響因子(影響力)學(xué)科排名
書目名稱Hypoxia網(wǎng)絡(luò)公開度
書目名稱Hypoxia網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Hypoxia被引頻次
書目名稱Hypoxia被引頻次學(xué)科排名
書目名稱Hypoxia年度引用
書目名稱Hypoxia年度引用學(xué)科排名
書目名稱Hypoxia讀者反饋
書目名稱Hypoxia讀者反饋學(xué)科排名
作者: 激勵 時間: 2025-3-21 21:23
,Widerst?nde und ihre Werkstoffe,ls and characteristics based on varying the copy number of HREs in response to cellular hypoxia. We explain how to clone HREs into luciferase reporter constructs in the sense, antisense, and dual directions to measure luciferase expression for functional analyses.作者: 圓桶 時間: 2025-3-22 01:58 作者: 高原 時間: 2025-3-22 05:49
Wie Frauen erfolgreich in Führung gehento hypoxia impact the metabolic phenotype of cancer cells. Such adaptations include increased glucose uptake, increased lactate production, and increased levels of other metabolites that stabilize HIF-1α, leading to a vicious circle of hypoxia-induced tumor growth.作者: 容易生皺紋 時間: 2025-3-22 08:57
1064-3745 ing-edge, .Hypoxia: Methods and Protocols .is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies...?.978-1-0716-3635-0978-1-0716-3633-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AGOG 時間: 2025-3-22 15:59 作者: Lumbar-Spine 時間: 2025-3-22 17:08 作者: mortgage 時間: 2025-3-22 22:02
Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivoand magnetic resonance imaging (MRI) using electromagnetic waves, are ideal..Therefore, as an advanced protocol for this research purpose, we provide ex vivo and in vivo methods to investigate the genetic response of multiple copies of hypoxia response elements (HREs) to tumor hypoxia in terms of in作者: monochromatic 時間: 2025-3-23 03:58 作者: 含沙射影 時間: 2025-3-23 06:23
https://doi.org/10.1007/978-3-322-87701-7l enters oxygen-rich regions within a tissue. Here, we describe a unique approach to permanently mark cells that experience hypoxia with a fluorescent protein switch that is maintained even after a cell is reoxygenated. This method consists of a dual-viral delivery system that can be transduced into any mammalian cell line.作者: 哭得清醒了 時間: 2025-3-23 11:50 作者: PATRI 時間: 2025-3-23 15:20
1064-3745 ation advice from the experts.This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in t作者: Misgiving 時間: 2025-3-23 18:58
https://doi.org/10.1007/978-3-658-36698-8 Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.作者: 共同給與 時間: 2025-3-23 22:40
,Elektrische Mobilit?ts-systeme (Antriebe),ced. Accordingly, assessment of HIF-1α protein levels and HIF transcriptional activity serve as an indirect indicator of hypoxia. In this series of protocols, I describe three methods to probe the HIF pathway.作者: syring 時間: 2025-3-24 02:29
https://doi.org/10.1007/978-3-663-12888-5ich cells are grown, and optical cables are attached to the outside of the cell culture vessels and connected to a computer. Associated software allows for the real-time monitoring of pericellular oxygen during cell culture experiments. This procedure enhances the reproducibility and control of cell culture.作者: Foreshadow 時間: 2025-3-24 09:10
Das Recht des Jahresabschlusses,). While visual assessment is fundamental, various quantitative indices such as tumor-to-muscle ratio have also been proposed. Several novel hypoxia tracers have been invented to compensate for the limitations of [.F]FMISO.作者: mastopexy 時間: 2025-3-24 14:21
,Derzeitiger Stand der Führungspsychologie,ing antibodies such as Hypoxyprobe?. This chapter describes a method for using Hypoxyprobe? to detect hypoxic areas in frozen and FFPE mouse samples by immunofluorescence (IF) and immunohistochemistry (IHC) staining.作者: 哀求 時間: 2025-3-24 16:10 作者: Charlatan 時間: 2025-3-24 20:30
Wie Fremdes vertraut werden kannof essential metabolic enzymes under hypoxic conditions. This method can be applied to study MDSCs function, mimicking the hypoxic environment in vitro. This method can be utilized to investigate the critical metabolic alterations under a given tumor context and help evaluate the efficacy of metabolic-targeted therapies in the long run.作者: 果核 時間: 2025-3-25 02:06
Measuring Pericellular Oxygen Tension for In Vitro Cell Cultureich cells are grown, and optical cables are attached to the outside of the cell culture vessels and connected to a computer. Associated software allows for the real-time monitoring of pericellular oxygen during cell culture experiments. This procedure enhances the reproducibility and control of cell culture.作者: 小樣他閑聊 時間: 2025-3-25 06:02
A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography). While visual assessment is fundamental, various quantitative indices such as tumor-to-muscle ratio have also been proposed. Several novel hypoxia tracers have been invented to compensate for the limitations of [.F]FMISO.作者: 偏見 時間: 2025-3-25 09:23 作者: PSA-velocity 時間: 2025-3-25 13:27 作者: narcotic 時間: 2025-3-25 16:57 作者: appall 時間: 2025-3-25 22:18
Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.作者: Aerophagia 時間: 2025-3-26 03:11
Detecting Hypoxia-Inducible Factor Levels and Activityced. Accordingly, assessment of HIF-1α protein levels and HIF transcriptional activity serve as an indirect indicator of hypoxia. In this series of protocols, I describe three methods to probe the HIF pathway.作者: Immortal 時間: 2025-3-26 07:26 作者: 北極人 時間: 2025-3-26 10:46 作者: 桉樹 時間: 2025-3-26 14:28
https://doi.org/10.1007/978-3-642-99604-7 xenografted tumors. Thus, the generation of stably transfected transgenic tumor cell lines, the in vitro calibration of the genetically encoded sensors, the surgical procedures for orthotopic xenografting of tumors in mice, and workflows for the respective sample preparation and microscopy are outlined.作者: 去世 時間: 2025-3-26 20:49
https://doi.org/10.1007/978-3-658-43206-5aging) to rapidly image NQO1 activity in hypoxic and non-hypoxic cancer cells and tumors developed in live mouse xenograft models. The specificity of the dye for NQO1 in all three procedures was verified, and the methods should be useful for both in vitro and in vivo studies.作者: 信徒 時間: 2025-3-27 00:45
Genetically Encoded Reporters to Monitor Hypoxia xenografted tumors. Thus, the generation of stably transfected transgenic tumor cell lines, the in vitro calibration of the genetically encoded sensors, the surgical procedures for orthotopic xenografting of tumors in mice, and workflows for the respective sample preparation and microscopy are outlined.作者: 緊張過度 時間: 2025-3-27 03:03
Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Canceaging) to rapidly image NQO1 activity in hypoxic and non-hypoxic cancer cells and tumors developed in live mouse xenograft models. The specificity of the dye for NQO1 in all three procedures was verified, and the methods should be useful for both in vitro and in vivo studies.作者: 新奇 時間: 2025-3-27 05:30 作者: 分發(fā) 時間: 2025-3-27 13:13 作者: precede 時間: 2025-3-27 15:58
Mapping the Fate of Hypoxic Cells Using an Irreversible Fluorescent Switches its effect on cancer, normal processes like embryogenesis, or other pathologies such as ischemia, depend on hypoxia-regulated mechanisms. Given the degradable nature of HIF-1/2α in the presence of oxygen, defining the role of hypoxia in modeling biological processes becomes challenging when a cel作者: 音樂等 時間: 2025-3-27 21:39 作者: 包庇 時間: 2025-3-27 21:57
A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro processes, including hematopoiesis, angiogenesis, metabolic changes, cell growth, and apoptosis. Hypoxia-inducible factor-1 (HIF-1) binds to hypoxia response elements (HREs) to selectively induce the expression of various genes in response to hypoxia. Therefore, HREs have been used to develop hypox作者: 博愛家 時間: 2025-3-28 05:49 作者: separate 時間: 2025-3-28 08:39 作者: 徹底檢查 時間: 2025-3-28 12:30
Measuring Pericellular Oxygen Tension for In Vitro Cell Culturelled in conventional cell culture, although chambers that control oxygen in the surrounding gas phase are commercially available. In both cases, it is valuable to understand the pericellular oxygen tension (i.e., the oxygen concentration that cells experience) in cultures. Herein we describe a proce作者: 創(chuàng)作 時間: 2025-3-28 14:59 作者: 破布 時間: 2025-3-28 19:50 作者: 傳染 時間: 2025-3-29 00:30 作者: Outwit 時間: 2025-3-29 05:22 作者: 玉米棒子 時間: 2025-3-29 09:49 作者: Pamphlet 時間: 2025-3-29 13:20 作者: 莊嚴(yán) 時間: 2025-3-29 17:22
Measurement of Metabolic Alteration in Immune Cells Under Hypoxiad expansion. Myeloid-derived suppressor cells (MDSCs) are heterogenous immature myeloid cells in the TME, which play an essential role in immune evasion by subverting T/NK cell-mediated killing. The immunosuppressive function of MDSCs is tightly regulated to the metabolic pathways, in which hypoxia 作者: 發(fā)誓放棄 時間: 2025-3-29 22:17
https://doi.org/10.1007/978-3-642-99604-7processes, tissue maintenance, and the development of pathologies. Fluorescence imaging using genetically encoded reporters enables hypoxia and oxygen imaging with cellular resolution. Thereby unrestricted visualization of hypoxic cells and regions essentially relies on the availability of oxygen-in作者: CRAB 時間: 2025-3-30 00:34 作者: 閑逛 時間: 2025-3-30 07:48
https://doi.org/10.1007/978-3-322-87701-7es its effect on cancer, normal processes like embryogenesis, or other pathologies such as ischemia, depend on hypoxia-regulated mechanisms. Given the degradable nature of HIF-1/2α in the presence of oxygen, defining the role of hypoxia in modeling biological processes becomes challenging when a cel作者: 柏樹 時間: 2025-3-30 11:00
https://doi.org/10.1007/978-3-658-43206-5diagnostic, and therapeutic settings. The scant expression of the antioxidant enzyme NQO1 in normal tissues and its great abundance in malignant counterparts due to the increased redox stress and hypoxia is one such example. Previously, we described a potent nontoxic probe that remains nonfluorescen作者: 談判 時間: 2025-3-30 12:46 作者: LVAD360 時間: 2025-3-30 18:42
https://doi.org/10.1007/978-3-658-36698-8 phosphorescent oxygen probes is a highly sensitive, noninvasive optical technique for the assessment of molecular oxygen in living cells and tissues. Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in co
