作者: 苦澀 時(shí)間: 2025-3-21 21:05 作者: 冒失 時(shí)間: 2025-3-22 02:07
High-Voltage Fast-Switching Gate Driversulated in an intracellular signaling network. Protein hydroxylation, which is a posttranslational modification catalyzed by oxygen-dependent enzymes, is a crucial regulator of protein-protein interactions. Under low oxygen conditions, the activity of many hydroxylases is inhibited, which results in 作者: PANG 時(shí)間: 2025-3-22 06:20 作者: 極端的正確性 時(shí)間: 2025-3-22 11:44 作者: AWRY 時(shí)間: 2025-3-22 13:23 作者: Painstaking 時(shí)間: 2025-3-22 18:48 作者: Evolve 時(shí)間: 2025-3-22 23:17
Wider den Purismus der Vernunft alterations in NAD(P)H/NAD(P). and intracellular ATP levels, and increased utilization of glutamine as the major precursor for fatty acid synthesis. In this chapter, we describe in detail various physiological assays that have been adopted to study the metabolic shift propagated by exposure to hypo作者: synovitis 時(shí)間: 2025-3-23 02:45 作者: Ethics 時(shí)間: 2025-3-23 08:59 作者: 舉止粗野的人 時(shí)間: 2025-3-24 00:43
https://doi.org/10.1007/978-3-663-01633-5s in an increase of red blood cell (RBC) production and delivery of more oxygen to tissues. Upon rapid return to normoxia, hypoxia-induced polycythemia is overcorrected by neocytolysis, a transient destruction of preferentially young RBCs bearing low catalase (downregulated by hypoxia-stimulated mic作者: 啞巴 時(shí)間: 2025-3-24 02:54
https://doi.org/10.1007/978-3-663-05587-7hod to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and作者: 青少年 時(shí)間: 2025-3-24 10:08 作者: 有害 時(shí)間: 2025-3-24 11:28 作者: Allodynia 時(shí)間: 2025-3-24 16:21 作者: 打擊 時(shí)間: 2025-3-24 23:04
Kinetic Analysis of HIF Prolyl Hydroxylases,tion rate, and inhibitory properties of the isoenzymes in vitro. Here we describe an assay measuring the substrate hydroxylation-coupled decarboxylation of radioactive 2-oxoglutarate to radioactive carbon dioxide as a fast, efficient, and diverse method to analyze the enzyme kinetics of HIF-P4Hs.作者: 聰明 時(shí)間: 2025-3-25 02:48
Mass Spectrometry and Bioinformatic Analysis of Hydroxylation-Dependent Protein-Protein Interactionulated in an intracellular signaling network. Protein hydroxylation, which is a posttranslational modification catalyzed by oxygen-dependent enzymes, is a crucial regulator of protein-protein interactions. Under low oxygen conditions, the activity of many hydroxylases is inhibited, which results in 作者: 潛伏期 時(shí)間: 2025-3-25 04:19
Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay,ition of dynamic and high-throughput data on HIF transcriptional activity during hypoxia and pharmacological activation of HIF. The sensitivity of the assay allows for the secreted luciferase to be consecutively sampled (as little as 1% of the total supernatant) over an extended time period, thus al作者: Finasteride 時(shí)間: 2025-3-25 10:42
Fluorescence Lifetime Imaging Microscopy (FLIM) as a Tool to Investigate Hypoxia-Induced Protein-Prr fluorophore in close proximity to an appropriate acceptor fluorophore transfers emission energy to the acceptor, resulting in a shorter lifetime of the donor fluorescence. When the respective FRET donor and acceptor are fused with two proteins of interest, a reduction in donor lifetime, as detecte作者: Dealing 時(shí)間: 2025-3-25 14:58
Transcriptional Profiling Using RNA-Seq to Study Hypoxia-Mediated Gene Regulation,he transcription factors known as hypoxia-inducible factors (HIFs). HIF1, a heterodimer of hypoxia-stabilized subunit HIF-1alpha and a constitutively expressed subunit HIF-1beta, serves as a key transcription factor that regulates gene expressions which are involved in cell growth, metabolism, and p作者: EPT 時(shí)間: 2025-3-25 19:53 作者: Omniscient 時(shí)間: 2025-3-25 23:10
Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells, alterations in NAD(P)H/NAD(P). and intracellular ATP levels, and increased utilization of glutamine as the major precursor for fatty acid synthesis. In this chapter, we describe in detail various physiological assays that have been adopted to study the metabolic shift propagated by exposure to hypo作者: Painstaking 時(shí)間: 2025-3-26 03:07 作者: 腐敗 時(shí)間: 2025-3-26 08:01 作者: defenses 時(shí)間: 2025-3-26 09:52 作者: 高興去去 時(shí)間: 2025-3-26 16:36 作者: Conduit 時(shí)間: 2025-3-26 18:36 作者: BLAZE 時(shí)間: 2025-3-26 22:37 作者: 未完成 時(shí)間: 2025-3-27 01:24
Hypoxic Treatment of Zebrafish Embryos and Larvae,hod to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and作者: 侵害 時(shí)間: 2025-3-27 08:52
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.作者: contrast-medium 時(shí)間: 2025-3-27 13:26 作者: 口味 時(shí)間: 2025-3-27 16:33 作者: candle 時(shí)間: 2025-3-27 20:36
https://doi.org/10.1057/978-1-349-94969-4ables us to understand the comprehensive regulation of gene expression by specific factors or environmental stimuli. Here, we describe the complete process of studying hypoxia-mediated gene expression by using RNA-Seq, including the hypoxic treatment of cells, RNA isolation, RNA quality check, cDNA library preparation, and library quality check.作者: 過(guò)去分詞 時(shí)間: 2025-3-28 01:57 作者: nitric-oxide 時(shí)間: 2025-3-28 06:02
Transcriptional Profiling Using RNA-Seq to Study Hypoxia-Mediated Gene Regulation,ables us to understand the comprehensive regulation of gene expression by specific factors or environmental stimuli. Here, we describe the complete process of studying hypoxia-mediated gene expression by using RNA-Seq, including the hypoxic treatment of cells, RNA isolation, RNA quality check, cDNA library preparation, and library quality check.作者: 好色 時(shí)間: 2025-3-28 08:18 作者: 有效 時(shí)間: 2025-3-28 12:17 作者: 大罵 時(shí)間: 2025-3-28 17:38
https://doi.org/10.1007/978-3-663-05587-7devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and can be applied to other fish and amphibian species.作者: badinage 時(shí)間: 2025-3-28 18:46 作者: 使顯得不重要 時(shí)間: 2025-3-29 02:31
https://doi.org/10.1007/978-3-658-43713-8ging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.作者: Infect 時(shí)間: 2025-3-29 06:50 作者: Ardent 時(shí)間: 2025-3-29 09:12 作者: PALSY 時(shí)間: 2025-3-29 12:37 作者: GEM 時(shí)間: 2025-3-29 17:21
Wider den Purismus der Vernunftxic conditions in pancreatic cell culture model that includes glucose uptake, glutamine uptake, and lactate release by pancreatic cancer cell lines. We have also elaborated the assays to evaluate the ratio of NAD(P)H/NAD(P). and intracellular ATP estimation using the commercially available kit to assess the metabolic state of cancer cells.作者: 蝕刻 時(shí)間: 2025-3-29 20:22
Die sozialistische Sicht der Ehe,y used approach for understanding the mechanisms of hypoxic sensing by the carotid body. Here, we describe the detailed methodology for recording action potential signals from the carotid sinus nerve from in vivo and ex vivo carotid bodies from rats.作者: 偶像 時(shí)間: 2025-3-30 03:01
Wolfgang Kr?nert,Paul Piotrowskistigate biological processes, regulating cellular adaptations. Here we describe detailed protocols for hypoxia exposure of pregnant rodent models and low oxygen exposure of trophoblast stem cells, utilizing gas-regulated chamber systems. The presentation also includes phenotypic analyses of the manipulated animal models and cells.作者: assail 時(shí)間: 2025-3-30 06:23
https://doi.org/10.1007/978-3-663-01633-5roRNA(miR)-21) caused by reactive oxygen species (ROS) from expanded mitochondria. In order to study molecular mechanism of neocytolysis, it is critical to differentiate life span of young and old RBCs and to measure the hematological changes before and after hypoxia treatment. Here we describe the methodological aspects of these measurements.作者: mendacity 時(shí)間: 2025-3-30 10:30
Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells,xic conditions in pancreatic cell culture model that includes glucose uptake, glutamine uptake, and lactate release by pancreatic cancer cell lines. We have also elaborated the assays to evaluate the ratio of NAD(P)H/NAD(P). and intracellular ATP estimation using the commercially available kit to assess the metabolic state of cancer cells.作者: hegemony 時(shí)間: 2025-3-30 16:15 作者: Regurgitation 時(shí)間: 2025-3-30 19:50 作者: 碎石頭 時(shí)間: 2025-3-30 21:17
Evaluation of Erythrocyte Changes After Normoxic Return from Hypoxia,roRNA(miR)-21) caused by reactive oxygen species (ROS) from expanded mitochondria. In order to study molecular mechanism of neocytolysis, it is critical to differentiate life span of young and old RBCs and to measure the hematological changes before and after hypoxia treatment. Here we describe the methodological aspects of these measurements.作者: 音樂(lè)戲劇 時(shí)間: 2025-3-31 00:53 作者: FUME 時(shí)間: 2025-3-31 08:11 作者: obtuse 時(shí)間: 2025-3-31 09:59 作者: 防水 時(shí)間: 2025-3-31 16:13 作者: outrage 時(shí)間: 2025-3-31 18:35
,?Der Nomade setzt seine Wanderung fort?,The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.作者: 必死 時(shí)間: 2025-4-1 01:15
Literaturwissenschaft / KulturwissenschaftImmunohistochemistry (IHC) enables the detection and distribution of proteins in cells of tissues. IHC has become an indispensable approach for studying oxygen sensing by the carotid body (CB). This chapter provides a detailed description of IHC of CB tissue and isolated CB cells.作者: acquisition 時(shí)間: 2025-4-1 01:58
Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells,The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.作者: Nutrient 時(shí)間: 2025-4-1 06:50
Immunohistochemistry of the Carotid Body,Immunohistochemistry (IHC) enables the detection and distribution of proteins in cells of tissues. IHC has become an indispensable approach for studying oxygen sensing by the carotid body (CB). This chapter provides a detailed description of IHC of CB tissue and isolated CB cells.作者: adulterant 時(shí)間: 2025-4-1 13:17
L. Eric HuangIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 背景 時(shí)間: 2025-4-1 15:12 作者: 朝圣者 時(shí)間: 2025-4-1 19:34 作者: 滑動(dòng) 時(shí)間: 2025-4-2 00:59
https://doi.org/10.1007/978-3-662-24697-9me. Herein, we outline protocols optimized to detect binding of Hypoxia-Inducible Factor (HIF)-1α or HIF-2α to putative hypoxia response elements (HREs) within HIF target genes expressed in breast tumor epithelial cells.作者: aggressor 時(shí)間: 2025-4-2 04:06 作者: 問(wèn)到了燒瓶 時(shí)間: 2025-4-2 10:21 作者: 圖畫(huà)文字 時(shí)間: 2025-4-2 14:58
