標(biāo)題: Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Microbial Quantitati Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2017 Springer-Ve [打印本頁(yè)] 作者: DEIFY 時(shí)間: 2025-3-21 18:50
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作者: Nonflammable 時(shí)間: 2025-3-22 00:10 作者: Inordinate 時(shí)間: 2025-3-22 02:31 作者: Latency 時(shí)間: 2025-3-22 07:08 作者: GRATE 時(shí)間: 2025-3-22 08:50 作者: 煩憂 時(shí)間: 2025-3-22 13:32 作者: 壓倒性勝利 時(shí)間: 2025-3-22 18:12 作者: Encephalitis 時(shí)間: 2025-3-23 01:01 作者: BLAZE 時(shí)間: 2025-3-23 03:56
Christoph C. Tebbe,Anja B. Dohrmann,Michael Hemkemeyer,Astrid N?therf biodiversity.Characterizes the emergence and self-organiza.Species are not functionally independent. From a long-term perspective, only ecosystem with a fully integrated nutrient cycle is alive. The lack of trophic autonomy should be considered one of the key factors that ensure and maintain biodi作者: 歹徒 時(shí)間: 2025-3-23 06:27 作者: 變白 時(shí)間: 2025-3-23 10:16
Alex J. Dumbrell,Robert M. W. Ferguson,Dave R. Clarknce of which have just lately been fully realized. Today we know that "biological diversity is a global asset of incalculable value to present and future generations" (Kofi Annan). Biodiversity is spread unequally over the world: in fact, the main share of biological resources worldwide is harboured作者: 膽汁 時(shí)間: 2025-3-23 14:40
Yolanda Blanco,Mercedes Moreno-Paz,Jacobo Aguirre,Victor Parroly depends on genetic change by recombination which — in the higher forms of life — occurs during sexual reproduction and by natural selection, reducing the genetically produced large numbers of individuals. None of the extant species and their individuals can exist without hundreds of interrelation作者: 總 時(shí)間: 2025-3-23 21:01 作者: 抱負(fù) 時(shí)間: 2025-3-23 22:11 作者: 圣人 時(shí)間: 2025-3-24 03:07 作者: Palter 時(shí)間: 2025-3-24 09:33 作者: hurricane 時(shí)間: 2025-3-24 11:02 作者: 騷擾 時(shí)間: 2025-3-24 17:54
Real-Time PCR Approaches for Analysis of Hydrocarbon-Degrading Bacterial Communities,ed greatly. However, quantification of particular environmental microbes by “endpoint PCR” techniques has typically been inaccurate due to inherent limitations and biases introduced during amplification. Such problems were overcome in the 1990s following the development of “real-time PCR” methods th作者: Fretful 時(shí)間: 2025-3-24 22:00 作者: Encumber 時(shí)間: 2025-3-25 02:25
Denaturing Gradient Gel Electrophoresis (DGGE) for Microbial Community Analysis,namics. The method is rapid and affordable, allowing multiple samples to be processed simultaneously. This protocol provides a background to the theory and progress in DGGE techniques and offers a detailed step-by-step procedure for laboratories employing DGGE for the analysis of microbial communiti作者: 角斗士 時(shí)間: 2025-3-25 07:09
Microbial Community Profiling: SSCP and T-RFLP Techniques,inting/profiling the diversity of microbial communities by distinction of similar-sized DNA fragments, amplified from directly extracted environmental DNA with PCR primers, hybridizing to phylogenetically conserved regions of microbial genomes. Since their introduction to microbial ecology, both met作者: concise 時(shí)間: 2025-3-25 07:43
Clone Libraries of Ribosomal RNA Gene Sequences for Characterization of Microbial Communities, expensive and time-consuming than community fingerprinting techniques, sequence analysis of clone libraries provides a high level of phylogenetic resolution due to long read lengths generated by Sanger sequencing. In this chapter, we provide the principles and methodologies of clone library constru作者: 小隔間 時(shí)間: 2025-3-25 14:44 作者: ALIBI 時(shí)間: 2025-3-25 17:22
Multiplex Fluorescent Antibody Microarrays and Antibody , for Microbial and Biomarker Detection in ntal samples. An FSMI consists basically in two steps: (i) specific binding of antigens (or any recognizable analyte in a sample) to the immobilized antibodies (.) on a microarray and (ii) revealing specific antigen-antibody binding by fluorescently labelled antibodies (.). Because multiplexing immu作者: 冒號(hào) 時(shí)間: 2025-3-25 20:38
Studying Protistan Communities in Hydrocarbon-Contaminated Environments,tly, much of our knowledge regarding the distribution, taxonomic diversity, and activity of protists relied on classical observation and cultivation methods. The application of molecular biological approaches which are based on the analysis of phylogenetic marker genes, such as the 18S ribosomal RNA作者: 指耕作 時(shí)間: 2025-3-26 01:58
1949-2448 ooting advice.Features practical laboratory guidelines to pr.This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell sepa作者: 殺子女者 時(shí)間: 2025-3-26 07:40
Introduction to Microbial Quantitation, Community Profiling, and Array Approaches,ive experimental design and analysis. Microbial community analysis is in a boom era and the protocols in this volume reflect the wide range of methods available for use by microbiologists to better understand microbial communities in environmental samples.作者: 別名 時(shí)間: 2025-3-26 08:37
Microbial Biomass and Community Composition Analysis Using Phospholipid Fatty Acids,as gas chromatography and mass spectroscopy. The quantitative nature of the resulting data allows for the application of statistics that authenticate microbial biomass and composition differences across an environment or between treatments.作者: 愛花花兒憤怒 時(shí)間: 2025-3-26 14:56
Denaturing Gradient Gel Electrophoresis (DGGE) for Microbial Community Analysis,ngerprint bands for sequencing. All required reagents and supplies are listed, as are the sequences of the most commonly used PCR primers for DGGE of bacterial, archaeal, and eukaryotic 16S rRNA gene amplicons.作者: pacific 時(shí)間: 2025-3-26 18:12 作者: Ovulation 時(shí)間: 2025-3-26 20:58 作者: incarcerate 時(shí)間: 2025-3-27 04:25 作者: Foment 時(shí)間: 2025-3-27 07:35
Michael Lappé,Jens Kallmeyerexperimental approaches alongside experimental ones. In a broad perspective, irrespective of the difficulty of testing largely unreproducible phenomena such as historical contingency (Gould 1989), consideration of both evolutionary and contemporary processes is crucial to a better understanding of t作者: 無(wú)情 時(shí)間: 2025-3-27 12:21 作者: infantile 時(shí)間: 2025-3-27 15:59
Yin Shen,Gerrit Voordouwes pest damage by making it difficult for specialist insects to move from host to host (Andow 1991). In this chapter, an example in which botanical diversity poses a problem for an herbivorous insect is reviewed. The nature of the insect’s predicament is described and behavioral strategies employed 作者: 門窗的側(cè)柱 時(shí)間: 2025-3-27 21:28 作者: Inferior 時(shí)間: 2025-3-28 01:29 作者: aviator 時(shí)間: 2025-3-28 04:44
Stefan J. Green,Mary Beth Leigh,Josh D. Neufeldry to herbivores and predators, parasites are much more specialised in terms of feeding and seldom can easily switch from one food source to another..Basically, there is no doubt that indirect mutually beneficial relations (that maintain diversity) are to be found throughout the entire ecological co作者: 慢跑 時(shí)間: 2025-3-28 07:29
Mary Beth Leigh,Lee Taylor,Josh D. Neufeld that these days very few experienced ecologists would doubt that species diversity not only stabilises the functional parameters of a guild (production, total biomass, etc.) but also tends to maximise these parameters in the process of self-arrangement, of course, to the extent possible with the av作者: receptors 時(shí)間: 2025-3-28 14:02
Alex J. Dumbrell,Robert M. W. Ferguson,Dave R. Clarked by the Senate of the University of Bonn in 1995, has played a central role in turning Bonn into a centre for international cooperation and North-South dialogue. The Centre is a product of the Bonn- Berlin agreement of July 1994 which was adopted to offset the effects caused by the Parliament and much of th978-3-642-08370-9978-3-662-06071-1作者: Generator 時(shí)間: 2025-3-28 18:20 作者: 否決 時(shí)間: 2025-3-28 22:27 作者: BLINK 時(shí)間: 2025-3-29 00:23
Christoph C. Tebbe,Anja B. Dohrmann,Michael Hemkemeyer,Astrid N?theropulations, and ecological communities..(c) in evolution, the emergence of biodiversity is determined by heritable variation and the advantage of specialised (more effective) structures over non-specialised one978-3-031-11584-4978-3-031-11582-0作者: anarchist 時(shí)間: 2025-3-29 06:25
1949-2448 turally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganism978-3-662-57081-4978-3-662-52778-8Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: 可卡 時(shí)間: 2025-3-29 10:09
A Cell Extraction Method for Oily Sediments,vent delivers highest cell counts. .-Hexane delivers best results for samples containing less biodegraded oil, whereas methanol turned out to be the most appropriate solvent for samples containing strongly biodegraded oil. The optimal solvent to sample ratio has to be determined prior to analysis fo作者: monopoly 時(shí)間: 2025-3-29 12:51
Introduction to Microplate MPN Enumeration of Hydrocarbon Degraders,degraders, monoaromatic hydrocarbon degraders, and diaromatic hydrocarbon degraders; (3) MPN enumeration of polycyclic aromatic hydrocarbon (PAH) degraders; (4) a radiotracer MPN method based on the conversion of .C-labeled substrate into .CO. which is quantified by autoradiography and digital image作者: inflame 時(shí)間: 2025-3-29 15:33
Primers for dsr Genes and Most Probable Number Method for Detection of Sulfate-Reducing Bacteria inversity and/or numbers of SRB in environmental samples. Although routinely used for research purposes, these methods are not yet used widely in the oil and gas industry to assess the presence of SRB and the success of mitigation measures.作者: Indigence 時(shí)間: 2025-3-29 21:46 作者: 閃光你我 時(shí)間: 2025-3-30 03:33 作者: Abrupt 時(shí)間: 2025-3-30 05:35
Clone Libraries of Ribosomal RNA Gene Sequences for Characterization of Microbial Communities,ormation of competent . cells, and screening of clones to verify the presence of cloned inserts, followed by DNA sequence analyses. Primers useful for phylogenetic analyses of bacterial, archaeal, and fungal communities are provided.作者: obsession 時(shí)間: 2025-3-30 11:10
Microbial Community Analysis by Single-Amplicon High-Throughput Next Generation Sequencing: Data An produce false conclusions. These various analytical steps and considerations can appear daunting to the uninitiated and may be perceived as a hurdle to completing research. In this chapter, we aim to provide the methods and guidance required to overcome this hurdle, and impart the skills required f作者: 遷移 時(shí)間: 2025-3-30 16:24
Multiplex Fluorescent Antibody Microarrays and Antibody , for Microbial and Biomarker Detection in ate antigen-antibody pair and other antibodies printed on the microarray. Then, deconvolution methods allow disentangling the cross-reaction events and discriminate between true cognate antigen-antibody recognition from others due to related antigenic structures. Therefore, the multiplex FSMI implem作者: 牙齒 時(shí)間: 2025-3-30 17:59
