標(biāo)題: Titlebook: Human Respiratory Syncytial Virus; Methods and Protocol Ralph A. Tripp,Patricia A. Jorquera Book 2016 Springer Science+Business Media New Y [打印本頁] 作者: deflate 時間: 2025-3-21 17:28
書目名稱Human Respiratory Syncytial Virus影響因子(影響力)
作者: temperate 時間: 2025-3-21 21:11 作者: 民間傳說 時間: 2025-3-22 01:58
Hayat Caidi,Jennifer L. Harcourt,Lia M. Hayneskroskopischen Untersuchungen an einigen . und . [. u. .; . u. . (1 u. 2)] nur aussagen, da? die losgel?sten Heterocysten sehr wahrscheinlich stoffwechselphysiologisch inaktiv und keimungsunf?hig sind. Ihre Substruktur ist n?mlich weitgehend desorganisiert. Die Bildung von Heterocysten scheint von de作者: 松緊帶 時間: 2025-3-22 06:35 作者: semiskilled 時間: 2025-3-22 11:39
Samadhan J. Jadhao,Larry J. Anderson M.D. Untersucht sind . sp. nov., . sp. nov., . und . Die acht bisher untersuchten Chrysochromulina-Arten zeigen eine weitgehende übereinstimmung in der Zellstruktur, bis auf kleine Abweichungen. So sind die Pyrenoide von . und . periplastidial, bei . und . intraplastidial. Die Struktur des Haptonemas is作者: declamation 時間: 2025-3-22 15:00 作者: regale 時間: 2025-3-22 17:59 作者: Outwit 時間: 2025-3-23 00:12
ochtemperaturwerkstoff mu? zunderbest?ndig sein und eine hohe Warmfestigkeit und gute Temperaturwechselbest?ndigkeit aufweisen. Die derzeit verwendeten sogenannten Superlegierungen auf Nickel-Chrom- und Kobalt-Chrom-Basis erlauben Arbeitstemperaturen bis h?chstens 870°. Die Verwendung der hochschmel作者: 熟練 時間: 2025-3-23 04:03
Human Respiratory Syncytial Virus: An Introduction,promised. Over the last decade many important mechanisms contributing to RSV infection, replication, and disease pathogenesis have been revealed; however, there is still insufficient knowledge which has in part hampered vaccine development. Considerable information is accumulating regarding how RSV 作者: configuration 時間: 2025-3-23 07:54
RSV Growth and Quantification by Microtitration and qRT-PCR Assays,virulence of the infection. Here, we describe a simple method, based on limiting dilution, for the removal of defective interfering particles from RSV. This method results in a high-titer viral preparation from both HEp-2 and Vero cell lines. We evaluated two concentrations of sucrose to stabilize t作者: 愛了嗎 時間: 2025-3-23 12:26
Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays,scribed. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV 作者: 增減字母法 時間: 2025-3-23 15:26 作者: 小淡水魚 時間: 2025-3-23 21:07 作者: 不怕任性 時間: 2025-3-24 00:05
Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection,and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as co作者: 輕而薄 時間: 2025-3-24 04:26 作者: 性學(xué)院 時間: 2025-3-24 09:34
In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airwayy adapted to infection of human hosts. Rodent models, such as mice and cotton rats, are semi-permissive to RSV infection and do not faithfully reproduce hallmarks of RSV disease in humans. Furthermore, immortalized airway-derived cell lines, such as HEp-2, BEAS-2B, and A549 cells, are poorly represe作者: 兇殘 時間: 2025-3-24 11:16 作者: forecast 時間: 2025-3-24 15:26
Use of Minigenome Systems to Study RSV Transcription,e, we describe the RSV minigenome assay for determining transcription by the viral polymerase in the absence of infection. We detail two different methods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quan作者: intangibility 時間: 2025-3-24 22:33
Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics,in. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed custom-made gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for binding to RSV M protein作者: Loathe 時間: 2025-3-24 23:48
A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellulaion of druggable cellular protein that are essential for RSV replication. In this regard experimental strategies that are able to screen relevant proteins from the vast array of proteins in the cellular milieu will facilitate the identification of potential drug targets. In this chapter we describe 作者: GEST 時間: 2025-3-25 06:44 作者: febrile 時間: 2025-3-25 10:47
Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus,the common cold and can go undiagnosed. However, infants as well as the elderly are at risk for developing severe cases, which can lead to high morbidity and mortality rates especially if there are underlying health issues. Despite many years of effort, no vaccine or specific treatments exist and RS作者: ATP861 時間: 2025-3-25 11:54
In Vivo Assessment of Airway Function in the Mouse Model,nvasive methods. Noninvasive methods can be used to monitor respiratory function in mice without the involvement of restraint, anesthesia, or surgery. The methods allow for multiple animals to be monitored simultaneously and can be used in longitudinal studies requiring repeated measurements on the 作者: albuminuria 時間: 2025-3-25 16:10 作者: perpetual 時間: 2025-3-26 00:02
978-1-4939-8117-5Springer Science+Business Media New York 2016作者: 和音 時間: 2025-3-26 03:01
Human Respiratory Syncytial Virus978-1-4939-3687-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 收集 時間: 2025-3-26 06:50
https://doi.org/10.1007/978-1-4939-3687-8virus growth; isolation; RSV virus; immune response; RSV infection; lower respiratory tract infections; in作者: 砍伐 時間: 2025-3-26 11:43
Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay,One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.作者: 漂浮 時間: 2025-3-26 14:50
Ralph A. Tripp,Patricia A. JorqueraIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 不能妥協(xié) 時間: 2025-3-26 20:46
Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/429424.jpg作者: Fortuitous 時間: 2025-3-27 00:33
as Madrider Symposium ?Plant-Water Relationships in Arid and Semi-arid Conditions“; auf einige der darin enthaltenen Gedanken wird noch eingegangen werden. Ein weiteres Symposium befa?te sich mit den Methoden der pflanzlichen ??kophysiologie“ (Montpellier 1962).作者: 瑣事 時間: 2025-3-27 03:41
Patricia A. Jorquera,Lydia Anderson,Ralph A. Tripp Ph.D.n und Text informieren kurz und sehr durchdacht. — Eine Zusammenstellung der bei Farnpflanzen bisher festgestellten chemischen Daten gibt das Handbuch von . in systematischer Anordnung. Leider sind die Farne chemisch oft noch recht wenig untersucht; einige ausgenommen, die als Heilpflanzen benutzt w作者: 無價值 時間: 2025-3-27 08:37
Hayat Caidi,Jennifer L. Harcourt,Lia M. Haynesn. Letztere bilden entweder unter Wachstum, Fragmentation und Sporulation die sporogene Phase oder nach Fusion wieder das Heterocystenstadium. Der Entwicklungsablauf in der Heterocystenphase ist von der Belichtung und vom Vorhandensein von Glucose im Kulturmedium [. u. . (1); . u. .] abh?ngig. In Du作者: Adulterate 時間: 2025-3-27 13:26
Patricia A. Jorquera Ph.D.,Ralph A. Tripp Ph.D. durch passive Ionendiffusionen entlang dem elektrochemischen Potentialgef?lle ebenfalls beschrieben werden, wobei die nach der Michaelis-Menten-Gleichung geforderten Geraden auch erhalten werden. Die Erh?hung der K-Aufnahme aus KC1 mit steigender Au?enkonzentration kann danach entweder durch verst?作者: 小隔間 時間: 2025-3-27 16:48
Samadhan J. Jadhao,Larry J. Anderson M.D.Schuppen l??t sich nur im Elektronenmikroskop analysieren. ?hnliche Schuppen konnten auch für die farblose . nachgewiesen werden, was systematisch wichtig ist. Bezüglich der Gei?elinsertion, der Beziehung des Zellkerns zur Gei?elwurzel und zu den Piastiden, der Struktur der Mitochondrien und des Gol作者: 含水層 時間: 2025-3-27 20:01
Samadhan J. Jadhao,Larry J. Anderson M.D.n und Text informieren kurz und sehr durchdacht. — Eine Zusammenstellung der bei Farnpflanzen bisher festgestellten chemischen Daten gibt das Handbuch von . in systematischer Anordnung. Leider sind die Farne chemisch oft noch recht wenig untersucht; einige ausgenommen, die als Heilpflanzen benutzt w作者: CRUDE 時間: 2025-3-27 23:32 作者: grotto 時間: 2025-3-28 04:00
en Angaben ist Zirkonborid als Düsenwerkstoff allen anderen Materialien überlegen. Besonders gute Zunderbest?ndigkeit haben einige Silicide, insbesondere das Molybd?ndisilicid. Durch Sintern hergestellte Formstücke aus diesem Material k?nnen an Luft bis auf 1700° erhitzt werden, ohne da? sie sich me作者: breadth 時間: 2025-3-28 10:06 作者: 錯 時間: 2025-3-28 11:09 作者: Heart-Rate 時間: 2025-3-28 14:38
Christopher C. Stobart,Anne L. Hotard,Jia Meng,Martin L. Moore作者: 來這真柔軟 時間: 2025-3-28 22:32 作者: 群居男女 時間: 2025-3-29 00:01 作者: 厭倦嗎你 時間: 2025-3-29 06:24
Cory J. Knudson,Kayla A. Weiss,Megan E. Stoley,Steven M. Varga作者: 使饑餓 時間: 2025-3-29 10:39
Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importanceic CK2α inhibitor 4,5,6,7-tetrabromobenzotriazole, revealing a similar reduction in viral titers as CK2α specific siRNA. This demonstrates that siRNA can be used to characterize critical host cell–RSV protein–protein interactions, and establishes CK2α as a future druggable target.作者: Alopecia-Areata 時間: 2025-3-29 14:40 作者: 多樣 時間: 2025-3-29 15:33
Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus,sessed with high resolution. While we focus on the T lymphocyte response in the lung, this technique can be readily adapted to examine various leukocytic cell types in the lung following RSV infection.作者: GRILL 時間: 2025-3-29 22:16 作者: languid 時間: 2025-3-30 00:37 作者: 使痛苦 時間: 2025-3-30 05:58 作者: Longitude 時間: 2025-3-30 09:30
Reverse Genetics of Respiratory Syncytial Virus,rently no available vaccines. Here, we describe the methods for recovery of recombinant RSV viruses using a bacterial artificial chromosome and methods related to procurement and expansion of stocks of RSV mutants.作者: anticipate 時間: 2025-3-30 13:11
Use of Minigenome Systems to Study RSV Transcription,hods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quantitative PCR method for determination of specific effects on the transcription of individual viral genes and the polar transcription gradient of RSV.作者: observatory 時間: 2025-3-30 16:56
Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus,ity and mortality rates especially if there are underlying health issues. Despite many years of effort, no vaccine or specific treatments exist and RSV is still the leading cause of infant hospitalizations worldwide. Here, we describe methods to infect two widely used small animal models: laboratory mice and cotton rats.作者: generic 時間: 2025-3-30 23:12 作者: 感情脆弱 時間: 2025-3-31 04:14 作者: canvass 時間: 2025-3-31 06:17
Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cell disease and for developing vaccines and access to purified, expressed proteins is important to RSV research and diagnostics. We describe methods to produce recombinant RSV F and G proteins in human cells and purify these proteins using Ni Sepharose affinity chromatography.作者: 雪上輕舟飛過 時間: 2025-3-31 09:38
Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficieyeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines, especially lines secreting neutralizing antibodies.
