作者: Immunoglobulin 時(shí)間: 2025-3-21 21:17 作者: 雜色 時(shí)間: 2025-3-22 03:26
HPV DNA Detection and Typing in Cervical Scrapes,en be detected by alkaline phosphatase conjugated anti-digoxigenin polyclonal antibodies and substrate followed by optical density reading. Subsequently, EIA-positive PCR products can be typed by a reverse line blot genotyping procedure that, using a miniblotter device, enables typing of up to 39 sa作者: Trabeculoplasty 時(shí)間: 2025-3-22 07:14
,Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures,mortalized HFK cell line that supports the complete HPV life cycle has further allowed investigators to study wild-type or mutant papillomaviral genomes that do not confer immortalization. In this chapter, we describe the methodologies that permit the study of the HPV life cycle in this HFK cell lin作者: Ischemic-Stroke 時(shí)間: 2025-3-22 10:24
ients do not receive the most effective therapy. Finally, various new treatment been developed, including medical treat- modalities have ment directed at endocrine pathways in the prostatic cells, balloon dilatation, spirals, temporary or permanent stents, C). nd the application of heat in hyperther作者: 確定方向 時(shí)間: 2025-3-22 15:25
Ethel-Michele de Villiers PhD,Corinna Whitley,Karin Gunstn a modified Foley-type catheter, could effectively heat periurethral tissues to a depth of about 0.5–1.0 cm (Chap. 13). When the applicator was installed intraurethrally and the feedback thermoregulation was set at 46°C, a cylindrical volume approximately 4 cm in length and 0.5 cm outward from the 作者: 人類 時(shí)間: 2025-3-22 17:28 作者: Melatonin 時(shí)間: 2025-3-22 21:19
Kiyofumi Egawakidney of an experimental animal recognize that its load has been doubled abruptly? What is the signal for renal hypertrophy? Benign hypertrophy of the prostate is quite different from compensatory hypertrophy of the kidney. It is now known that benign prostatic hypertrophy (BPH) is not a hypertrophy but a be978-1-4612-5478-2978-1-4612-5476-8作者: Assemble 時(shí)間: 2025-3-23 03:27 作者: 精致 時(shí)間: 2025-3-23 07:33
Woei Ling Peh PhD,John Doorbar PhDkidney of an experimental animal recognize that its load has been doubled abruptly? What is the signal for renal hypertrophy? Benign hypertrophy of the prostate is quite different from compensatory hypertrophy of the kidney. It is now known that benign prostatic hypertrophy (BPH) is not a hypertrophy but a be978-1-4612-5478-2978-1-4612-5476-8作者: monogamy 時(shí)間: 2025-3-23 12:07
Rashmi Seth,John Rippin,Li Guo,David Jenkins MD, FRCPathce: Education in and beyond the Age of Reason is designed to disturb the reader. Education constitutes us as subjects; we owe our existence to its violent inscriptions. Those who refuse or rebel against our educational present must begin by objecting to the subjects we have become..978-1-137-59392-4978-1-137-27286-7作者: debase 時(shí)間: 2025-3-23 16:54
Svetlana Vinokurova,Nicolas Wentzensen,Magnus von Knebel Doeberitzce: Education in and beyond the Age of Reason is designed to disturb the reader. Education constitutes us as subjects; we owe our existence to its violent inscriptions. Those who refuse or rebel against our educational present must begin by objecting to the subjects we have become..978-1-137-59392-4978-1-137-27286-7作者: 斥責(zé) 時(shí)間: 2025-3-23 19:31 作者: 容易懂得 時(shí)間: 2025-3-24 00:19 作者: Addictive 時(shí)間: 2025-3-24 03:23 作者: 600 時(shí)間: 2025-3-24 07:11
Regina Wilson BS,Laimonis A. Laimins PhDe Risikoabw?gung (unbehandelte Krankheitsbefunde vs. Behandlungsrisiko) und letztlich einer Kostenabw?gung. Das Spektrum der von Patienten mit klinischer BPH angegebenen Symptome ist breit und wird im allgemeinen in 2 Gruppen getrennt: obstruktive und irritative Symptome (Tabelle 4.1). Beide Symptom作者: FAWN 時(shí)間: 2025-3-24 14:05 作者: 安慰 時(shí)間: 2025-3-24 18:33
N. Sanjib Banerjee PhD,Louise T. Chow PhD,Thomas R. Broker PhDagnostischen Wert bei BPH zuordneten.[11]. Letztlich hat auch der Nachweis einer fehlenden Korrelation zwischen Symptomatik oder Prostatagr??e mit der urodynamisch nachweisbaren Obstruktion zu einer neuen Definition der Aussagef?higkeit der Urethrozystoskopie bei BPH geführt.作者: 競選運(yùn)動(dòng) 時(shí)間: 2025-3-24 19:32
William Bonnez MDe Risikoabw?gung (unbehandelte Krankheitsbefunde vs. Behandlungsrisiko) und letztlich einer Kostenabw?gung. Das Spektrum der von Patienten mit klinischer BPH angegebenen Symptome ist breit und wird im allgemeinen in 2 Gruppen getrennt: obstruktive und irritative Symptome (Tabelle 4.1). Beide Symptom作者: 阻撓 時(shí)間: 2025-3-24 23:25
Janet L. Brandsma PhDagnostischen Wert bei BPH zuordneten.[11]. Letztlich hat auch der Nachweis einer fehlenden Korrelation zwischen Symptomatik oder Prostatagr??e mit der urodynamisch nachweisbaren Obstruktion zu einer neuen Definition der Aussagef?higkeit der Urethrozystoskopie bei BPH geführt.作者: paleolithic 時(shí)間: 2025-3-25 06:40 作者: 新鮮 時(shí)間: 2025-3-25 10:09
Peter C. Angeletti PhDstrointestinale St?rungen auf – ?hnlich denen, die bei der Achalasie bekannt sind. Der Kandidatengen-Ansatz hat vielversprechende Hinweise nahegelegt, wenngleich bis zum heutigen Zeitpunkt noch keine schlüssige Verlinkung zu spezifischen Genen etabliert werden konnte. Welche Teilaspekte hierbei ents作者: Exhilarate 時(shí)間: 2025-3-25 12:10 作者: 橢圓 時(shí)間: 2025-3-25 16:07
Methods in Molecular Medicinehttp://image.papertrans.cn/h/image/429349.jpg作者: 混亂生活 時(shí)間: 2025-3-25 22:53
https://doi.org/10.1385/1592599826DNA; PCR; Southern Blot; Virus; cell lines; gene expression; genes; infection; infections; infectious; sacchar作者: N防腐劑 時(shí)間: 2025-3-26 02:17
Histochemical Analysis of Cutaneous HPV-Associated Lesions,ous human papillomavirus-associated lesions. Every step of the staining procedure, from sampling of the specimens to microscopic examination of the stained sections, is detailed with reference to the recent achievements in this field.作者: DEBT 時(shí)間: 2025-3-26 05:59 作者: Pcos971 時(shí)間: 2025-3-26 11:28
The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease,ails use of the cottontail rabbit papillomavirus (CRPV)-laboratory rabbit model. The protocols describe how to infect rabbits with CRPV DNA or CRPV virus to induce papillomas. They also describe the design and analysis of genetic pathogenesis experiments, prophylactic and therapeutic intervention experiments, and malignant progression experiments.作者: Exaggerate 時(shí)間: 2025-3-26 15:36 作者: Conclave 時(shí)間: 2025-3-26 17:38
1543-1894 ing-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle,作者: Efflorescent 時(shí)間: 2025-3-26 23:59 作者: 易于交談 時(shí)間: 2025-3-27 01:12
Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypodology of retrovirus-mediated gene transfer, which can transduce the viral gene or genes into an entire population of PHKs. In this chapter, we describe the strategies and methods of retrovirus-mediated gene transfer into keratinocytes grown into organotypic cultures.作者: Vasoconstrictor 時(shí)間: 2025-3-27 08:45 作者: 六邊形 時(shí)間: 2025-3-27 12:48
Identification of HPV Variants,A→G mutation of the E6 ORF, using restriction fragment length polymorphism assays . In addition, we describe approaches for DNA sequencing and analysis. Such methods are likely to be of particular interest to those involved in epidemiological investigations of virus transmission and pathogenicity studies.作者: cutlery 時(shí)間: 2025-3-27 16:37 作者: 改正 時(shí)間: 2025-3-27 21:15 作者: jarring 時(shí)間: 2025-3-27 22:40
Replication and Encapsidation of Papillomaviruses in ,,east provide a potentially high-efficiency means to produce large quantities of infectious virus in a short time frame. Fourth, assembly of HPV virus in yeast allows encapsidation of mutant genomes, since previous studies have shown that no viral ORF is required for replication of full-length HPV in yeast.作者: ingrate 時(shí)間: 2025-3-28 02:35
Identification of New Papillomavirus Types,ere. The CP, FAP, and GP primers are used for PCR amplification, followed by cloning and sequencing of the amplicons. Sequence comparisons and the interpretation of DNA sequence identities are discussed. Details of defining a new papillomavirus type and of the recently approved taxonomic classification system for the . are given.作者: 協(xié)議 時(shí)間: 2025-3-28 06:32
Detection and Quantitation of HPV Gene Expression Using Real-Time PCR,sed to investigate the expression of HPV 16 early genes in HG or LG precancer are demonstrated. Detecting the expression of early HPV genes in conjunction with the Pap smear may improve the specificity of identifying LG precancers that are associated with high risk of progression.作者: 疼死我了 時(shí)間: 2025-3-28 11:29
Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers,ression of p16. is induced by the viral oncoprotein E7 and distinguishes dysplastic lesions from benign changes. Integration of human papillomavirus DNA into the host genome is mainly found in high-grade dysplastic lesions and invasive cancers, and points to an increased progression potential.作者: 毀壞 時(shí)間: 2025-3-28 17:53
,Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture,cycle. In this chapter, we describe in detail how to (1) culture keratinocytes, (2) electroporate primary keratinocytes with HPV DNA, (3) detect episomal HPV genomes by Southern (DNA) blotting, (4) grow organotypic raft cultures, (5) isolate HPV, and (6) perform in vitro infectivity testing.作者: 直言不諱 時(shí)間: 2025-3-28 20:05 作者: Senescent 時(shí)間: 2025-3-29 00:46 作者: ingestion 時(shí)間: 2025-3-29 04:57 作者: 字形刻痕 時(shí)間: 2025-3-29 08:15 作者: 無彈性 時(shí)間: 2025-3-29 11:24 作者: LAVE 時(shí)間: 2025-3-29 16:50
Book 2006study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze pa作者: 甜得發(fā)膩 時(shí)間: 2025-3-29 23:13
Identification of New Papillomavirus Types,f these infections to a multiplicity of clinical manifestations. The cloning and sequencing of PCR-amplified products has, to date, resulted in the identification of more than 300 putative “new” papillomavirus types. The methods used to identify these unknown papillomavirus sequences are described h作者: micronutrients 時(shí)間: 2025-3-30 00:25 作者: 注意到 時(shí)間: 2025-3-30 06:12
Histochemical Analysis of Cutaneous HPV-Associated Lesions,ous human papillomavirus-associated lesions. Every step of the staining procedure, from sampling of the specimens to microscopic examination of the stained sections, is detailed with reference to the recent achievements in this field.作者: defray 時(shí)間: 2025-3-30 09:45 作者: 能量守恒 時(shí)間: 2025-3-30 16:10
Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections,muno-detection varies for different proteins and is dependent on antigen availability. Epitope exposure is sometimes necessary, because the antigen may become masked after formalin fixation and paraffin embedding of the infected tissue. Visualization of both nucleic acid and protein targets can be d作者: arousal 時(shí)間: 2025-3-30 19:00 作者: 輕浮女 時(shí)間: 2025-3-30 21:46
Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers,rvical cancer development, but there is a high percentage of healthy women infected with HPV that never develop a lesion. Only a small percentage of low-grade dysplasias finally grow out to invasive cancer. Several biomarkers can be used to identify lesions at risk for malignant progression. Overexp作者: consent 時(shí)間: 2025-3-31 01:01 作者: 加劇 時(shí)間: 2025-3-31 06:45 作者: obstruct 時(shí)間: 2025-3-31 11:48 作者: Humble 時(shí)間: 2025-3-31 14:08 作者: 做事過頭 時(shí)間: 2025-3-31 18:15 作者: Incisor 時(shí)間: 2025-3-31 21:49
,Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose,of HPV virions, isolated from tissues, to study viral pathogenesis has been complicated due to the low numbers of HPV virions synthesized and inefficient infection of cells in tissue culture. As an alternative approach, genetic methods have been developed to study the papillomavirus life cycle in it作者: 結(jié)果 時(shí)間: 2025-4-1 03:22 作者: 大量 時(shí)間: 2025-4-1 06:45 作者: 沙漠 時(shí)間: 2025-4-1 13:17 作者: Obverse 時(shí)間: 2025-4-1 17:25
The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease,ails use of the cottontail rabbit papillomavirus (CRPV)-laboratory rabbit model. The protocols describe how to infect rabbits with CRPV DNA or CRPV virus to induce papillomas. They also describe the design and analysis of genetic pathogenesis experiments, prophylactic and therapeutic intervention ex作者: NAVEN 時(shí)間: 2025-4-1 20:31
Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology,at both HPV type 16 (HPV-16) and type 31b (HPV-31b) productively replicate in a trophoblast cell line, 3A. Trophoblasts are an important cell type, as these are the cells of the placenta that directly contact maternal tissues. There are a number of studies that suggest that HPV infection may be foun
