標題: Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 2016Latest edition Springer Science+Business Media New York 2016 Derivation of ce [打印本頁] 作者: Flippant 時間: 2025-3-21 16:52
書目名稱Human Embryonic Stem Cell Protocols影響因子(影響力)
作者: GIBE 時間: 2025-3-21 22:57
Jeffrey Fergus,Rene Quintanilla,Uma Lakshmipathymodel of an application into a fully-functional C# project, including designing the user interface, implementing the business logic, and integrating with a relational database for data storage. Along the way, you will explore the .NET Framework, the creation of a Windows-based user interface, a web-作者: 合唱隊 時間: 2025-3-22 02:39 作者: 乳白光 時間: 2025-3-22 07:15
Tomonori Tsuyama,Nobuaki Shiraki Ph.D.,Shoen Kume Ph.D.rom Visual Studio except IDE. EWDK also allows to use MASM64 (Microsoft Macro-Assembly) and C# compilers. ..With the aid of the MSBuild System, one can compile Visual Studio Projects (.vcxproj) and Solutions (.sln) without even using Visual Studio! Similarly, MinGW compilers can be deployed from 7z/作者: 減弱不好 時間: 2025-3-22 10:46
Sravanti Kusuma,Sharon Gerechtefine variables and make decisions.Work with arrays and loops, pointers and references, strings, and more.Write your own functions, types, and operators.Discover the essentials of object-oriented programming.Use overloading, inheritance, virtual functions and polymorphism.Write generic function temp作者: chalice 時間: 2025-3-22 14:18
Peter S. Vestentoft,Christian B. Br?chner,Niels Lynnerup,Claus Yding Andersen,Kjeld M?llg?rd essential support for C++23. After completing this book, you will have the knowledge and skills needed to build your first C++ applications.?.What You Will Learn.Begin programming with the C++23 standard.Carry out modular programming in C++.Work with arrays and loops, pointers and references, strin作者: EXALT 時間: 2025-3-22 19:31 作者: Amnesty 時間: 2025-3-22 22:16
1064-3745 man Embryonic Stem Cell Protocols, Third Edition. serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting..978-1-4939-4998-4978-1-4939-2668-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 裂口 時間: 2025-3-23 04:30 作者: 笨拙的你 時間: 2025-3-23 06:36 作者: 點燃 時間: 2025-3-23 09:45
,Characterizing Pluripotent Stem Cells Using the TaqMan? hPSC ScorecardTM Panel,onments. Recently, genetic and epigenetic expression signatures were used to define and confirm cell states, thus providing a surrogate molecular assay that can potentially replace complex in vivo cellular assays such as teratoma formation..In this chapter, we describe a molecular assay for rapid ch作者: 狂熱文化 時間: 2025-3-23 17:11
Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix,eder layers and animal ingredients, thereby limiting the risk of pathogenic contamination and additionally, facilitating their manipulation. Herein we present an improved version of our previous protocol for extracting ECM from human foreskin fibroblast using a different buffer. Our new hypotonic sh作者: 透明 時間: 2025-3-23 20:35
Genetic Manipulation of Human Embryonic Stem Cells,ethodologies that have been applied for the genetic manipulation of HESCs and their applications. Detailed protocols for generating clones of genetically modified HESCs by transfection, electroporation, and infection will be described, with special emphasis on the important technical details that ar作者: parsimony 時間: 2025-3-24 02:07
Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 Sy, Nat Biotechnol 31:227–229, 2013; Friedland et al., Nat Methods 10(8):741–743, 2013; DiCarlo et al., Nucleic Acids Res 41:4336–4343, 2013; Cong et al., Science 339:819–823, 2013) has greatly accelerated the development of human genome manipulation at the molecular level. This chapter describes the 作者: gerontocracy 時間: 2025-3-24 04:22 作者: 橫截,橫斷 時間: 2025-3-24 07:06
Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based of an entire colony is accomplished using 3D image processing software such as Mimics. or Amira.. An extended version of this technique even allows for a high-magnification 3D-reconstruction of, e.g., hepatic stem cells in developing liver. These techniques combined allow for both a 2- and a 3-dime作者: 廣告 時間: 2025-3-24 11:08
Cara K. Bradley,Julia Schaft,Tammie K. Roy,Biljana Dumevska,Teija T. Peuranterface, implementing the business logic, and integrating with a relational database for data storage. Along the way, you will explore the .NET Framework, the creation of a Windows-based user interface, a web-978-1-4302-3530-9978-1-4302-3531-6作者: 吹氣 時間: 2025-3-24 16:48
Andres Sanz-Garcia,Miodrag Stojkovic,Carmen Escobedo-Luceanterface, implementing the business logic, and integrating with a relational database for data storage. Along the way, you will explore the .NET Framework, the creation of a Windows-based user interface, a web-978-1-4302-3530-9978-1-4302-3531-6作者: 委派 時間: 2025-3-24 22:28 作者: 規(guī)范就好 時間: 2025-3-25 02:58 作者: Confirm 時間: 2025-3-25 06:11 作者: 翅膀拍動 時間: 2025-3-25 08:00
Vlasta Lungova,Ciara Leydon,Susan Thibeaulthe C++20 language.?.What You Will Learn.Begin programming with the C++20 standard.Carry out modular programming in C++.Work with arrays and loops, pointers and references, strings, and more.Write your own funct978-1-4842-5884-2作者: 吸氣 時間: 2025-3-25 15:43 作者: orient 時間: 2025-3-25 16:41 作者: anticipate 時間: 2025-3-25 22:57
https://doi.org/10.1007/978-1-4939-2668-8Derivation of cells; Epithelial cells; HESCs; Pluripotent cell differentiation; Regenerative medicine; St作者: 放牧 時間: 2025-3-26 02:20 作者: 草本植物 時間: 2025-3-26 07:26 作者: CYT 時間: 2025-3-26 10:38
Kursad TurksenIncludes cutting-edge methods and protocols involving human embryonic stem cells.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the e作者: LAY 時間: 2025-3-26 15:22 作者: venous-leak 時間: 2025-3-26 18:43
1064-3745 ults.Contains key notes and implementation advice from the e.The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume c作者: 放肆的你 時間: 2025-3-27 00:57
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR,t how to apply the RT-PCR technique, protocols for its performance, experimental setup, and software analysis, as of the gene expression of hESC lines in consecutive passages for long-term culture surveillance.作者: Calibrate 時間: 2025-3-27 03:05 作者: –scent 時間: 2025-3-27 06:28 作者: 殺蟲劑 時間: 2025-3-27 10:50
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonenewal of HESCs. We also describe a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix Microarrays. These techniques will provide us unprecedented scale of biological information that would illuminate a key to decipher complex signaling networks controlling pluripotency.作者: 無法治愈 時間: 2025-3-27 13:53
Study of Gap Junctions in Human Embryonic Stem Cells,ommon characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap junctions in hESC, from expression of gap junction proteins to functional study of GJIC in hESC proliferation, apoptosis, colony growth, and pluripotency.作者: 的闡明 時間: 2025-3-27 20:13 作者: uveitis 時間: 2025-3-28 01:10 作者: 感情脆弱 時間: 2025-3-28 06:06 作者: 自愛 時間: 2025-3-28 07:06
Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate,m self-renewal of hPSCs in defined, xeno-free medium with dissociated single-cell passaging. Here, we describe a modified culture system with similar performance to efficiently expand hPSCs under defined, xeno-free conditions using a non-biological synthetic substrate.作者: 尖 時間: 2025-3-28 12:38 作者: 友好關系 時間: 2025-3-28 15:03
Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Eliminati(d) 8 to d10. Methionine deprivation results in elimination of undifferentiated cells from the culture with no significant loss of definitive endoderm cells, as compared to those cultured under complete condition throughout the whole culture period.作者: Arteriography 時間: 2025-3-28 19:14 作者: 起來了 時間: 2025-3-29 02:46
Book 2016Latest editionnd expert tips on troubleshooting and avoiding known pitfalls..Thorough and practical, .Human Embryonic Stem Cell Protocols, Third Edition. serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting..作者: Gerontology 時間: 2025-3-29 04:07 作者: 廚師 時間: 2025-3-29 10:41
Book 2016Latest editionl biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful .Methods in Molecular Biology. series format, chapters inclu作者: Folklore 時間: 2025-3-29 13:39 作者: 新手 時間: 2025-3-29 15:46 作者: Altitude 時間: 2025-3-29 20:24 作者: malapropism 時間: 2025-3-30 03:43 作者: 控訴 時間: 2025-3-30 08:00
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonxpand undifferentiated HESCs, and the latter can be used for the preparation of pure HESCs RNA samples, or for screening factors influential on self-renewal of HESCs. We also describe a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix Microarrays. These techniqu作者: Concrete 時間: 2025-3-30 10:43
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR,ve quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeeping control with the addition of a calibrator that serves as an internal control to compare different lots of reactions during the time. The main aspects will include a minimal numb作者: insidious 時間: 2025-3-30 13:32
Study of Gap Junctions in Human Embryonic Stem Cells,iological events. GJIC is required for mouse embryonic stem cell maintenance and proliferation and various studies suggest that functional GJIC is a common characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap jun作者: Diskectomy 時間: 2025-3-30 18:45 作者: ORE 時間: 2025-3-30 21:30 作者: GIST 時間: 2025-3-31 03:51
Genetic Manipulation of Human Embryonic Stem Cells, genetic modifications while remaining pluripotent, and can be selectively propagated, allowing the clonal expansion of genetically altered cells in culture. Since the first isolation of ES cells in mice, many effective techniques have been developed for gene delivery and manipulation of ES cells. T作者: Hiatus 時間: 2025-3-31 08:52
Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 Syced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861–872, 2007) provides a renewable source of patient autologous cells that not only retain identical genetic information but also give rise to many cell types of the body including neurons and glia. Meanwhile, the rap作者: acetylcholine 時間: 2025-3-31 10:54
Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentive cells. Here we describe a multicolor flow cytometry technique that uses five distinct cell surface antigens to isolate four live populations with different surface antigen profiles. These profiles were used to help distinguishing between neural and nonneural (the lens) ectoderm derivatives withi作者: orient 時間: 2025-3-31 14:09 作者: 察覺 時間: 2025-3-31 21:11 作者: 統(tǒng)治人類 時間: 2025-3-31 22:01 作者: 收藏品 時間: 2025-4-1 01:59
Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosahy larynx without engendering a significant and unacceptable risk to vocal fold function. Here, we describe the procedure to create an engineered vocal fold tissue construct consisting of the scaffold composed of the collagen 1 gel seeded with human fibroblasts and simple epithelial progenitors seed作者: Ingenuity 時間: 2025-4-1 06:46
Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based ress transcription factors OCT4, SOX2, and NANOG, and surface markers SSEA-4 and TRA-1-60 and TRA-1-81 and their ability to differentiate into derivatives of the three germ layers show the differentiating potential. Studies suggest a certain microheterogeneity of the hESC colonies, in which not all
