作者: Omniscient 時間: 2025-3-21 20:33
Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.作者: 節(jié)省 時間: 2025-3-22 03:46
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryong factors influential on self-renewal of hESCs. Also described in this chapter: a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix microarrays. These techniques will provide an unprecedented scale of biological information to illuminate a key to decipher complex signaling networks controlling pluripotency.作者: 神刊 時間: 2025-3-22 07:27 作者: 逃避現(xiàn)實 時間: 2025-3-22 09:12
Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells,by trypsin/ethylenediaminetetraacetic acid-mediated dissociation. We also describe methods we use to characterize hES cells expanded on either human or mouse feeder cells, including alkaline phosphatase staining, immunostaining for cell-surface markers associated with undifferentiated hES cells, and teratoma formation in mice.作者: 投射 時間: 2025-3-22 16:46 作者: flex336 時間: 2025-3-22 20:12
1064-3745 the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-59745-046-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: lymphoma 時間: 2025-3-23 00:27
In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells,ation accounts for at least 70% of the total differentiated progenies. Thus, our system not only provides a source of synchronized neuroectodermal cells, but also offers a paradigm to dissect mechanisms of neural induction and cell lineage specification during early human development.作者: 發(fā)生 時間: 2025-3-23 05:23 作者: dissent 時間: 2025-3-23 06:11 作者: Eosinophils 時間: 2025-3-23 12:21 作者: ANTI 時間: 2025-3-23 15:16 作者: 進步 時間: 2025-3-23 20:40
Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonid that this coculture system can induce dopaminergic differentiation in human embryonic stem cells, and the human embryonic stem cell-derived dopaminergic cells exhibit biochemical and functional properties of mature dopaminergic neurons.作者: POINT 時間: 2025-3-24 01:29
Book 20061st editionular Biology? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.作者: Ejaculate 時間: 2025-3-24 04:00 作者: Abjure 時間: 2025-3-24 06:31 作者: 戰(zhàn)役 時間: 2025-3-24 12:30
Kursad TurksenIncludes supplementary material: 作者: 實施生效 時間: 2025-3-24 18:41
Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/429132.jpg作者: Lacunar-Stroke 時間: 2025-3-24 22:07 作者: BROOK 時間: 2025-3-25 01:23
Designing, Testing, and Validating a Microarray for Stem Cell Characterization,Microarray technology is a powerful tool that allows for simultaneous assessment of the expression of thousands of genes and identification of gene expression patterns associated with specific cell types. Here we describe a protocol using this method to examine stem cells.作者: implore 時間: 2025-3-25 05:02
Massively Parallel Signature Sequencing,nd, therefore, is ideal for whole genome analysis. When applied to expression profiling, it reveals almost every transcript in the sample and provides its accurate expression level. This chapter describes the technology and its application in establishing stem cell transcriptome databases.作者: ostrish 時間: 2025-3-25 08:35
https://doi.org/10.1385/1597450464DNA; biology; cancer; cell; microarray; mitochondria; molecular biology; stem cell; stem cells; tissue; Cell c作者: pulse-pressure 時間: 2025-3-25 11:44 作者: 帶來 時間: 2025-3-25 16:55 作者: 母豬 時間: 2025-3-25 20:50 作者: 易碎 時間: 2025-3-26 03:00 作者: Biguanides 時間: 2025-3-26 06:28 作者: infelicitous 時間: 2025-3-26 10:55
Maisam Mitalipova,Gail Palmariniare so many options, especially in a language like C#. If you want to really get the best from a programming language, you need to know which features work best in which situations and understand their strengths and weaknesses. It is this understanding that makes the difference between coding and co作者: vanquish 時間: 2025-3-26 13:39
Jim McWhir,Davina Wojtacha,Alison Thomsonare so many options, especially in a language like C#. If you want to really get the best from a programming language, you need to know which features work best in which situations and understand their strengths and weaknesses. It is this understanding that makes the difference between coding and co作者: mortgage 時間: 2025-3-26 20:28 作者: BILIO 時間: 2025-3-26 21:06
Michal Amit,Joseph Itskovitz-Eldorany options, especially in a .NET language like C#. If you want to really get the best from a programming language, you need to know which features work best in which situations, and understand their strengths and weaknesses. It is this understanding that makes the difference between coding and codi作者: OPINE 時間: 2025-3-27 04:39 作者: Nucleate 時間: 2025-3-27 09:20
Ronald S. Goldsteinen by an experienced author team in a classic Apress Beginne.Updated with the changes to C#, .Beginning C# 2008 Objects: From Concepts to Code. introduces complete beginners to C# coding practice with a solid methodological foundation written by two critically–acclaimed experts in the field, already作者: pulse-pressure 時間: 2025-3-27 12:51 作者: 鉗子 時間: 2025-3-27 15:25
Xue-Jun Li,Su-Chun Zhangen by an experienced author team in a classic Apress Beginne.Updated with the changes to C#, .Beginning C# 2008 Objects: From Concepts to Code. introduces complete beginners to C# coding practice with a solid methodological foundation written by two critically–acclaimed experts in the field, already作者: 一回合 時間: 2025-3-27 19:14
Lisheng Wang,Chantal Cerdan,Pablo Menendez,Mickie Bhatiadatabase oriented programs using T-SQL and C#..Assuming only basic knowledge of C#, .Beginning C.#. 5.0 Databases. teaches all the fundamentals of database technology and database programming readers need to quickly become highly proficient database users and application developers..A comprehensive 作者: 煩擾 時間: 2025-3-28 01:24
Pablo Menendez,Lisheng Wang,Chantal Cerdan,Mickie Bhatiadatabase oriented programs using T-SQL and C#..Assuming only basic knowledge of C#, .Beginning C.#. 5.0 Databases. teaches all the fundamentals of database technology and database programming readers need to quickly become highly proficient database users and application developers..A comprehensive 作者: 隱士 時間: 2025-3-28 04:48 作者: Indebted 時間: 2025-3-28 07:37 作者: ciliary-body 時間: 2025-3-28 13:04 作者: 清洗 時間: 2025-3-28 17:13 作者: anticipate 時間: 2025-3-28 19:20
Human Embryonic Stem Cells,tement and promise in the field of regenerative medicine. However, along with great enthusiasm came hot contro-versy for stem cell research and researchers alike because available hES cell lines were isolated from “excess” embryos from in vitro fertilization clinics. Despite ethical and political de作者: vascular 時間: 2025-3-28 23:21
Blastocyst Culture for Deriving Human Embryonic Stem Cells,wn from frozen-thawed d 1-3 surplus embryos left over from infertility clinics and donated for stem cell research with informed patient consent. Knowledge, therefore, of the culture conditions and embryo scoring systems that are used to generate high-quality blastocysts are crucial. This chapter des作者: Ingenuity 時間: 2025-3-29 03:52 作者: 自然環(huán)境 時間: 2025-3-29 08:29
Derivation and Maintenance of Human Embryonic Stem Cells,dologies for their isolation and maintenance to exhaust the potential use of these unique cells in cell-based therapy and developmental research. To date, there are more than 50 reported well-characterized hESC lines worldwide. hESCs are traditionally isolated from the blastocysts on mouse embryonic作者: hauteur 時間: 2025-3-29 12:41
Isolation and Characterization of Human Embryonic Stem Cells,ed, involving mechanical or enzymatic passaging using either collagenase or trypsin. This chapter describes detailed protocols that have been used for the derivation, maintenance, and characterization of hES cells in vitro along with protocols to test their differentiation potential in vivo. When us作者: Intend 時間: 2025-3-29 17:52 作者: 嬉耍 時間: 2025-3-29 22:16
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditionsratology, and cellbased therapies. To allow their continuous growth as undifferentiated cells, isolation and culturing were traditionally conducted on mouse embryonic fibroblast feeder layers, using medium supplemented with fetal bovine serum. However, these conditions allow possible exposure of the作者: 幸福愉悅感 時間: 2025-3-30 02:57 作者: 不整齊 時間: 2025-3-30 05:04 作者: Fabric 時間: 2025-3-30 11:17 作者: forecast 時間: 2025-3-30 12:46 作者: 佛刊 時間: 2025-3-30 20:31
In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells, colony culture. ES cell colonies are detached intact from the fibroblast feeder layer using dispase or collagenase. The ES cell aggregates, after 4-6 d in suspension culture, are adhered to the culture surface and form colonies of monolayer in a chemically defined medium. Under this culture conditi作者: 赦免 時間: 2025-3-30 22:06 作者: 羅盤 時間: 2025-3-31 03:38 作者: 無彈性 時間: 2025-3-31 06:22
Genetic Manipulation of Human Embryonic Stem Cells by Transfection, genetic modifications while remaining pluripotent, and can be selectively propagated, allowing the clonal expansion of genetically altered cells in culture. Since the first isolation of ES cells in mice, many effective techniques have been developed for gene delivery and manipulation of ES cells. T作者: 量被毀壞 時間: 2025-3-31 12:01
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonr of which is used to maintain or expand undifferentiated hESCs, and the latter can be used for preparation of pure hESCs RNA samples, or for screening factors influential on self-renewal of hESCs. Also described in this chapter: a protocol and tips for conducting gene chip analysis focusing on wide作者: 有法律效應 時間: 2025-3-31 15:40 作者: 不成比例 時間: 2025-3-31 19:54
Human Embryonic Stem Cells, protocols for the potential future clinical application of hES cells. After the scientific and political obstacles are overcome, it is anticipated that the hES cell field will make a tremendous difference in conditions, such as burn traumas and diabetic foot ulcers, as well a number of degenerative作者: 無能的人 時間: 2025-3-31 22:49
Transplantation of Human Embryonic Stem Cells to the Chick Embryo, and integrate into the tissues of the chick embryo. Some of the hESCs differentiate and the type of embryonic microenvironment that the implanted cells were exposed to modified their differentiation. Therefore, this hESC-chick embryo system has potential for complementing studies in rodents and in 作者: 一條卷發(fā) 時間: 2025-4-1 03:43
Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells,inafter CD45.PFV) and are solely responsible for hematopoietic cell fate. Human ESC-derived hematopoietic cells have similar colony and cellular morphologies to those derived from committed adult hematopoietic tissues, and also show repopulating capacity in immune deficient mice after intrabone marr作者: Acclaim 時間: 2025-4-1 09:18 作者: 沒有貧窮 時間: 2025-4-1 11:59 作者: engender 時間: 2025-4-1 17:34
