作者: CREEK 時(shí)間: 2025-3-21 21:58
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules,The kidney is an extremely heterogeneous organ from a morphological and a functional point of view. In spite of this heterogeneity, several methods have been described in an attempt to isolate and culture homogeneous populations of renal epithelial cells, seeking to maintain normal differentiated characteristics of the nephron segment of origin.作者: maintenance 時(shí)間: 2025-3-22 01:52
Joanna PicotIncludes supplementary material: 作者: averse 時(shí)間: 2025-3-22 07:10 作者: Tortuous 時(shí)間: 2025-3-22 09:09
Human Cell Culture Protocols978-1-59259-861-8Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: RLS898 時(shí)間: 2025-3-22 13:48 作者: defeatist 時(shí)間: 2025-3-22 20:22 作者: MOAN 時(shí)間: 2025-3-22 23:02
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters,idermal melanocytes has been attempted since 1957 (.-.), but only since 1982 have pure normal human melanocyte cultures been reproducibly established to yield cells in sufficient quantity for biological, biochemical, and molecular analyses (.). Selective growth of melanocytes, which comprise only 3-作者: 令人不快 時(shí)間: 2025-3-23 03:59
Isolation and Culture of Human Osteoblasts,dal cell rich in organelles involved in the synthesis and secretion of matrix proteins. (.) Osteocyte-an osteoblast with low metabolic activity that has been engulfed in matrix during bone formation and entombed in lacunae. (.) Bone-lining cell-osteoblasts that have avoided entombment in lacunae and作者: Perennial長期的 時(shí)間: 2025-3-23 05:34
Human Osteoclast Culture From Peripheral Blood Monocytes,cells and, therefore, they cannot simply divide and be maintained in culturean osteoclast supply must constantly be replenished. Osteoclasts are large, multinucleated cells formed by the fusion of osteoclast precursors found in the monocyte fraction of blood. The osteoclast is thought to be the only作者: 格子架 時(shí)間: 2025-3-23 12:10
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation,ity (.). The markers of mature articular chondrocytes are type II collagen (COL2A1), other cartilage-specific collagens IX (COL9) and XI (COL11), the large aggregating proteoglycan aggrecan, and link protein (..). Chondrocytes also synthesize a number of small proteoglycans such as biglycan and deco作者: Sad570 時(shí)間: 2025-3-23 15:35 作者: Condense 時(shí)間: 2025-3-23 19:03
Cell Cultures of Autopsy-Derived Fibroblasts,eriments, which would help to elucidate the degenerative processes, cannot be performed with affected tissue. On the other hand, biopsy-derived human brain tissue from patients, as an alternative source for living cell material, is rare and, in autopsy-derived tissue, neuronal cells are generally al作者: 切碎 時(shí)間: 2025-3-23 23:24 作者: 嫻熟 時(shí)間: 2025-3-24 04:30
Purification of Peripheral Blood Natural Killer Cells,r lineages. NK cells are a discrete lymphocyte subset distinct from B and T cells based on both physical and phenotypic characteristics that can be exploited for their purification. Techniques based on differential cell buoyancy [centrifugation on discontinuous density gradients such as Percoll (.)]作者: APRON 時(shí)間: 2025-3-24 10:20
Human Fetal Brain Cell Culture,lls. Because of its cellular complexity, it is important to develop technical approaches that allow isolation and study of nerve and glial cells under conditions in which their environment can be precisely manipulated. Such methods have proven valuable in discovering the cellular and molecular mecha作者: 同時(shí)發(fā)生 時(shí)間: 2025-3-24 11:42
Culturing Human Schwann Cells,d methods used with other species (e.g., rat), simple dissociation of human peripheral nerve tissue has been found to result in both very low cell yields and poor Schwann cell purity (.,.). This is thought to be caused by the increased amount of connective tissue within human nerves and the mechanic作者: Vasoconstrictor 時(shí)間: 2025-3-24 17:04 作者: arbiter 時(shí)間: 2025-3-24 19:39
Isolation and Culture of Human Alveolar Epithelial Cells,lium differ drastically from those of the more distal, alveolar epithelium. This chapter will concentrate on the isolation and culture of human alveolar epithelial cells that line the peripheral gas exchange region of the lung. Whereas immortalized cell lines emanating mostly from the different cell作者: Temporal-Lobe 時(shí)間: 2025-3-24 23:30 作者: gerrymander 時(shí)間: 2025-3-25 06:56 作者: Distribution 時(shí)間: 2025-3-25 08:16 作者: 可能性 時(shí)間: 2025-3-25 12:24
Glomerular Epithelial and Mesangial Cell Culture and Characterization,nderstanding of the development of glomerular disease at the cellular level. Advances in our knowledge of the pathogenic mechanisms have made it clear that the response of intrinsic glomerular cells to external stimuli plays an important role in glomerular injury (.,.). Glomerular cells from several作者: Emasculate 時(shí)間: 2025-3-25 18:43
eory has become very large and shows the enormous progress that has been made. The mainstream literature is focused on frequentist statistical methods for - timating model parameters and evaluating model t. However, the Bayesian methodology has shown great potential, particularly for making further 作者: 消音器 時(shí)間: 2025-3-25 20:47
Claire Linge response models to handle more complex assessment data.CompThe modeling of item response data is governed by item response theory, also referred to as modern test theory. The eld of inquiry of item response theory has become very large and shows the enormous progress that has been made. The mainstr作者: LOPE 時(shí)間: 2025-3-26 02:34 作者: 嫌惡 時(shí)間: 2025-3-26 04:51 作者: 充足 時(shí)間: 2025-3-26 08:28
Katherine A. Buckley,Benjamin Y. Y. Chan,William D. Fraser,James A. Gallagher response models to handle more complex assessment data.CompThe modeling of item response data is governed by item response theory, also referred to as modern test theory. The eld of inquiry of item response theory has become very large and shows the enormous progress that has been made. The mainstr作者: 晚來的提名 時(shí)間: 2025-3-26 12:45
Mary B.eory has become very large and shows the enormous progress that has been made. The mainstream literature is focused on frequentist statistical methods for - timating model parameters and evaluating model t. However, the Bayesian methodology has shown great potential, particularly for making further 作者: 破布 時(shí)間: 2025-3-26 19:45 作者: Osteoporosis 時(shí)間: 2025-3-26 22:05 作者: 溫順 時(shí)間: 2025-3-27 03:53 作者: 無思維能力 時(shí)間: 2025-3-27 07:45 作者: foodstuff 時(shí)間: 2025-3-27 10:10 作者: 冰河期 時(shí)間: 2025-3-27 16:42
M. Leslie Fulcher,Sherif Gabriel,Kimberlie A. Burns,James R. Yankaskas,Scott H. Randellted volumes, the book forms a solid unity, with nearly 100 p.This book is an edited volume, the goal of which is to provide an overview of the current state-of-the-art in statistical methods applied to problems in structural bioinformatics (and in particular protein structure prediction, simulation,作者: 紋章 時(shí)間: 2025-3-27 18:55 作者: NUDGE 時(shí)間: 2025-3-27 22:28
Pierre Chailler,Daniel Ménardted volumes, the book forms a solid unity, with nearly 100 p.This book is an edited volume, the goal of which is to provide an overview of the current state-of-the-art in statistical methods applied to problems in structural bioinformatics (and in particular protein structure prediction, simulation,作者: multiply 時(shí)間: 2025-3-28 05:28 作者: FIN 時(shí)間: 2025-3-28 08:20 作者: 脆弱么 時(shí)間: 2025-3-28 13:58
Heather M. Wilson,Keith N. Stewartted volumes, the book forms a solid unity, with nearly 100 p.This book is an edited volume, the goal of which is to provide an overview of the current state-of-the-art in statistical methods applied to problems in structural bioinformatics (and in particular protein structure prediction, simulation,作者: 過份好問 時(shí)間: 2025-3-28 16:05
Gabrielle M. Hawksworthted volumes, the book forms a solid unity, with nearly 100 p.This book is an edited volume, the goal of which is to provide an overview of the current state-of-the-art in statistical methods applied to problems in structural bioinformatics (and in particular protein structure prediction, simulation,作者: coagulate 時(shí)間: 2025-3-28 20:50 作者: Heresy 時(shí)間: 2025-3-28 23:05
Cell Cultures of Autopsy-Derived Fibroblasts,advantage of using autopsyderived fibroblasts from deceased patients is that both the validity of the clinical diagnoses and the severity of neuropathological changes can be assessed reliably by subsequent histological investigations of the brain (.).作者: vertebrate 時(shí)間: 2025-3-29 06:15 作者: bronchiole 時(shí)間: 2025-3-29 08:32 作者: Lipoma 時(shí)間: 2025-3-29 11:45 作者: 冒失 時(shí)間: 2025-3-29 17:27 作者: extinguish 時(shí)間: 2025-3-29 20:58 作者: 木質(zhì) 時(shí)間: 2025-3-30 00:05
Book 2005Latest edition protocols follow the successful Methods in Molecular Medicine? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.作者: 辯論的終結(jié) 時(shí)間: 2025-3-30 07:55 作者: BARK 時(shí)間: 2025-3-30 09:28 作者: CAND 時(shí)間: 2025-3-30 13:57
1543-1894 aboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-61737-431-9978-1-59259-861-8Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: Missile 時(shí)間: 2025-3-30 16:52 作者: 滋養(yǎng) 時(shí)間: 2025-3-30 23:42
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters,cts on multiple cellular responses (.). Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to an effective and standardized isolation method, and special TPA-free culture media for selective growth and long-term maintenance of huma作者: 制造 時(shí)間: 2025-3-31 02:34 作者: 吹氣 時(shí)間: 2025-3-31 05:50
Human Myoblasts and Muscle-Derived SP Cells,erentiationspecific proteins. In this chapter, methods are given for the isolation of myoblasts from human muscle tissue using two different techniques: (a) flow cytometry (.) and (b) cell cloning (.,.). Recent reports have also highlighted the existence of highly primitive cells within mouse skelet作者: 接合 時(shí)間: 2025-3-31 11:48
Primary Culture and Differentiation of Human Adipocyte Precursor Cells,rleukin 6 (IL-6), as well as angiotensinogen and plasminogen activator inhibitor-1 (PAI-1) are some of the products secreted by adipocytes (.-.). When the balance between energy intake and expenditure is such that the intake exceeds the need of the body, adipose tissue expands and the individual bec作者: GUILE 時(shí)間: 2025-3-31 15:06
Human Fetal Brain Cell Culture,lack of an alternative source of viable normal human brain cells. Although research that employs human fetal tissue has been limited, what has been done has made a major impact in the development of prophylactics and therapeutics for several important human diseases. For example, the use of fetal ti作者: 一夫一妻制 時(shí)間: 2025-3-31 20:14 作者: CANE 時(shí)間: 2025-4-1 01:03
A New Approach to Primary Culture of Human Gastric Epithelium,wo different approaches, i.e., microsurgi-cal/scraping techniques and enzymatic treatments. The first requires laboratory expertise and, unless gradient fractionation or elutriation is used to purify glandular cells, these are rapidly overgrown during the first 2 d of culture by dividing mucous cell作者: 胰臟 時(shí)間: 2025-4-1 01:50 作者: Dawdle 時(shí)間: 2025-4-1 09:32
Isolation and Culture of Human Hepatocytes,cell membrane and cellular organelles are postulated to reflect the in vivo situation more closely. Hepatocytes represent 60-65% of the number of cells in the liver but occupy some 80% of the liver volume because of their large volume (.). Nonparenchymal cells such as Kuppfer cells, lipocytes, and e
