標(biāo)題: Titlebook: Host-Fungus Interactions; Methods and Protocol Alexandra C. Brand,Donna M. MacCallum Book 2012 Springer Science+Business Media, LLC 2012 Fu [打印本頁(yè)] 作者: expenditure 時(shí)間: 2025-3-21 17:31
書目名稱Host-Fungus Interactions影響因子(影響力)
作者: 記成螞蟻 時(shí)間: 2025-3-21 22:33 作者: Creditee 時(shí)間: 2025-3-22 02:22 作者: 呼吸 時(shí)間: 2025-3-22 04:48 作者: Diluge 時(shí)間: 2025-3-22 08:51
Megan D. Lenardon,André Nantelinclude audit location, audit attendees, time zones for participants, geographical locations, cultural considerations, and technology to be used for remote audits and technological capabilities of the auditee and various participants..Audit attendees should be strategically aligned to provide the in作者: 彩色的蠟筆 時(shí)間: 2025-3-22 13:49 作者: HALL 時(shí)間: 2025-3-22 19:48
Min Su Kim,Seo-Young Kim,Kwang-Woo Jung,Yong-Sun Bahn content with images and videos to accompany the text.Shares.This successor volume builds upon the first book with additional chapters meant to inspire readers to take action towards a healthier planet. It focuses on closing the gap between scientific insights on pressing environmental issues that d作者: 法律的瑕疵 時(shí)間: 2025-3-22 22:09
Lorina G. Baker,Jennifer K. Lodge content with images and videos to accompany the text.Shares.This successor volume builds upon the first book with additional chapters meant to inspire readers to take action towards a healthier planet. It focuses on closing the gap between scientific insights on pressing environmental issues that d作者: 誘惑 時(shí)間: 2025-3-23 02:21
Iran Malavazi,Gustavo Henrique Goldmanses on closing the gap between scientific insights on pressing environmental issues that do not often reach the general public, and putting that scientific knowledge in the hands of everyday people who can use these insights to take action against climate change, pollution, and biodiversity loss. Th作者: 猜忌 時(shí)間: 2025-3-23 06:28
Fabrice N. Gravelat,David S. Askew,Donald C. Sheppardal healthcare. It discusses key components of comprehensive sexual healthcare, emphasising the need for inclusivity, confidentiality and culturally sensitive approaches that address the well-being of individuals..The chapter examines barriers that hinder sexual health coverage in LMICs, highlighting作者: Camouflage 時(shí)間: 2025-3-23 12:37 作者: critique 時(shí)間: 2025-3-23 17:53 作者: –吃 時(shí)間: 2025-3-23 21:00 作者: 彎彎曲曲 時(shí)間: 2025-3-24 01:07
Jo?o F. Menino,Agostinho J. Almeida,Fernando Rodriguesden Age of Italian Cinema.” On the one hand it was a culmination of the auteur cinema, but on the other also a genuinely new step in genre cinema. In one year, 1960, Fellini’s L., Antonioni’s L.T. A. and Visconti’s R.R. H. B. arrived on the screen, followed in the next years and in the 1970s by othe作者: 發(fā)怨言 時(shí)間: 2025-3-24 06:05 作者: exceed 時(shí)間: 2025-3-24 08:51 作者: patriarch 時(shí)間: 2025-3-24 11:30
Lorina G. Baker,Jennifer K. Lodgeal part of research in the History of Science, guided by specific historiographical thesis. For a long time, ‘theoreticism’ had characterized historian’s interest. In the second half of the twentieth century alongside a na?ve empiricism widespread especially in scientific circles, the theory-ladenne作者: Confidential 時(shí)間: 2025-3-24 18:31 作者: 虛構(gòu)的東西 時(shí)間: 2025-3-24 22:49
s and program directors (PD) are a helpful resource, and medical students should meet with them once the decision is made to pursue a surgical specialty. Ideally, you will have experiences and accomplishments from your pre-clinical years that will enhance your application; however, we see many succe作者: Hirsutism 時(shí)間: 2025-3-25 01:12 作者: CANT 時(shí)間: 2025-3-25 06:20
Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in ,ng . gene and 200-bp flanking repeats that is useful for disruption of . genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the . gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function.作者: 并排上下 時(shí)間: 2025-3-25 09:38 作者: 刪除 時(shí)間: 2025-3-25 12:00 作者: FUME 時(shí)間: 2025-3-25 16:39
Targeted Gene Disruption in , Using Double-Joint PCR with Split Dominant Selectable Markersction of gene disruption cassettes carrying dominant selectable markers, followed by biolistic transformation. However, the conventional overlap PCR method between two flanking regions of the target gene and selectable marker is often inefficient due to the long length of the PCR product and the pre作者: lethal 時(shí)間: 2025-3-25 23:41
Multiple Gene Deletion in , Using the Cre–, Systemelectable markers available for use in making gene deletions or other genetic manipulations in .. Here, we describe the adaptation of the Bacteriophage P1 Cre–. system for use in ., and its application in the excision and reuse of the geneticin drug marker. This tool will allow investigators to make作者: CLAP 時(shí)間: 2025-3-26 01:42 作者: esoteric 時(shí)間: 2025-3-26 07:07
Targeted Gene Deletion in , Using the Hygromycin-Resistance Split-Marker Approach using site-specific recombination. Transformation of ., like many related fungal species, must overcome two major obstacles. First, the cell wall limits the entry of exogenous DNA, and second, a high rate of nonhomologous recombination leads to random ectopic integration of the marker. Here, we des作者: 藝術(shù) 時(shí)間: 2025-3-26 10:53
Gene Disruption in , Using Hygromycin or Phleomycin Resistance Markersarget gene of .. The DNA constructs employed contain either the gene that encodes for hygromycin B or phleomycin resistance, which are present in the pAN7.1 or pAN8.1 plasmid vectors, respectively. Hygromycin B or phleomycin are used to select for transformants at concentrations that inhibit growth 作者: seruting 時(shí)間: 2025-3-26 16:15 作者: Alcove 時(shí)間: 2025-3-26 17:33
RNA Interference in ,he process relies on double-stranded RNA (dsRNA) to target complementary messenger RNA for degradation. Here, we describe two plasmid-based strategies we have developed for RNAi in .. The pFrame vector utilizes the . promoter to enable the constitutive synthesis of hairpin RNA (hpRNA), the stem of w作者: 切碎 時(shí)間: 2025-3-26 23:49
Gene Knockdown in , Using Antisense RNAsms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in . by antisense RNA (aRNA) technology taking advantage of an .-mediated transformation (ATMT) system. T作者: 影響 時(shí)間: 2025-3-27 02:28
Tetracycline-Inducible Gene Expression in ,able promoters are available that enable researchers to shut off or turn on the expression of a target gene in ., usually by growing the cells in inducing or repressing media. In this chapter, we describe a tetracycline-inducible gene expression system (Tet-On) that allows forced expression of endog作者: Physiatrist 時(shí)間: 2025-3-27 05:27
Galactose-Inducible Promoters in , var. ,g gene essentiality in .. Galactose-inducible promoters have been successfully used in . to manipulate heterologous gene expression. Utilizing . galactose-inducible genes in a BLAST search of the sequenced . var. . genome, we found three potential galactose-inducible promoters, P., P., and P. that a作者: 赦免 時(shí)間: 2025-3-27 13:22 作者: Ordeal 時(shí)間: 2025-3-27 17:19 作者: FISC 時(shí)間: 2025-3-27 18:40 作者: Alopecia-Areata 時(shí)間: 2025-3-28 00:50 作者: 憤怒歷史 時(shí)間: 2025-3-28 02:18
1064-3745 d reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Comprehensive and practical, .Host-Fungus Interactions: Methods and Protocols. des978-1-4939-5904-4978-1-61779-539-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Lime石灰 時(shí)間: 2025-3-28 07:27
nd ended up being destined mostly to the colonial market. Hence, the serious crisis experienced by the sector since the late-eighteenth century due to the interruption of trade with America as a result of the revolutionary wars and the subsequent emancipation of those territories. But the drastic re作者: 使無(wú)效 時(shí)間: 2025-3-28 10:55 作者: olfction 時(shí)間: 2025-3-28 14:47 作者: STRIA 時(shí)間: 2025-3-28 20:47
Shantanu Ganguly,Aaron P. Mitchelltococcal infections at that time, was responsible for the deaths of 107 people. In early 1937, Sulfanilamide was used in powder and tablet form. Due to increased demand for a liquid form of the medication, the manufacturer, S.E. Massengill Company, decided to develop a liquid form of Sulfanilamide..作者: 收到 時(shí)間: 2025-3-28 23:55
Megan D. Lenardon,André Nantel. In fact, some auditors do not use any tools..The opening meeting of an audit sets the tone for the overall audit. The opening meeting should be focused and provide insight to the auditee as to how the lead auditor and audit team will approach the audit over the specified number of days.作者: oracle 時(shí)間: 2025-3-29 04:19
Iran Malavazi,Gustavo Henrique Goldmanrious environmental issues and solutions that are not often disseminated in popular discourse but are nonetheless important for non-scientific audiences to understand and become familiar with. They allow readers to explore the broad range of concepts and issues and recognize the far-reaching consequ作者: 改變 時(shí)間: 2025-3-29 08:32
Fabrice N. Gravelat,David S. Askew,Donald C. Sheppardfostering awareness about STI prevention. Additionally, the chapter investigates the implications of infertility on sexual health, considering ways it can impact on individuals and relationships..Lastly, the chapter explores the interconnectedness between gender-based violence and sexual health. It 作者: 抓住他投降 時(shí)間: 2025-3-29 11:23
as a charity, it officially stopped its propaganda efforts in 1963. Instead, it promoted eugenics through an annual symposium, joint lectures with other organizations and its publications. Significant amounts were channelled towards research, especially after the founding of the Marie Stopes Resear作者: 落葉劑 時(shí)間: 2025-3-29 18:36
Brian H. Youseff,Chad A. Rappleye as a charity, it officially stopped its propaganda efforts in 1963. Instead, it promoted eugenics through an annual symposium, joint lectures with other organizations and its publications. Significant amounts were channelled towards research, especially after the founding of the Marie Stopes Resear作者: 藐視 時(shí)間: 2025-3-29 22:34 作者: Infraction 時(shí)間: 2025-3-30 02:03
Jo?o F. Menino,Agostinho J. Almeida,Fernando RodriguesLuigi Zampa, and Vittorio De Sica, now often with a more satirical and bitter note. And finally, the 1960s gave birth to a new subgenre: the Italo-Western or Spaghetti-Western, and even the horror film and the sword-and-sandal movies had a strong revival. Thus, Italian film after 1960 was characteri作者: 影響帶來(lái) 時(shí)間: 2025-3-30 07:15
I interpreted the foundations of theoretical physics as constituted by two basic dichotomies: one about two kinds of mathematics, and another about two kinds of logic. Every theory relies on a couple of choices on the two dichotomies. I characterize the couple of choices of Maxwell-Hertz-Lorentz’ f作者: foreign 時(shí)間: 2025-3-30 10:42 作者: Explicate 時(shí)間: 2025-3-30 15:39
Lorina G. Baker,Jennifer K. Lodge purpose of detecting electromagnetic waves only by abandoning electromechanical instrumentation, and giving centrality to the resonator. In recent years Hanson and Amsterdamski provided a historiographical background for D’Agostino’s analysis about the relationships between History of science and H作者: adequate-intake 時(shí)間: 2025-3-30 17:44 作者: cardiac-arrest 時(shí)間: 2025-3-30 21:40 作者: triptans 時(shí)間: 2025-3-31 02:59 作者: 亞當(dāng)心理陰影 時(shí)間: 2025-3-31 06:10 作者: Lumbar-Stenosis 時(shí)間: 2025-3-31 12:37 作者: ULCER 時(shí)間: 2025-3-31 14:26
RNA Interference in ,ble interfering sequence to trigger RNAi in . and describe the steps for subcloning into either vector, transforming . by electroporation, screening clones for RNAi-related phenotypes, and evaluating the efficacy and specificity of gene silencing by RNAi.
