標(biāo)題: Titlebook: Homologous Recombination; Methods and Protocol Andrés Aguilera,Aura Carreira Book 2021 Springer Science+Business Media, LLC, part of Spring [打印本頁(yè)] 作者: 富裕 時(shí)間: 2025-3-21 17:08
書(shū)目名稱Homologous Recombination影響因子(影響力)
作者: 阻塞 時(shí)間: 2025-3-21 22:51 作者: CORE 時(shí)間: 2025-3-22 03:00 作者: subordinate 時(shí)間: 2025-3-22 07:51 作者: Vital-Signs 時(shí)間: 2025-3-22 11:30 作者: neolith 時(shí)間: 2025-3-22 13:29 作者: follicle 時(shí)間: 2025-3-22 20:01
Fabio Vanoli,Rohit Prakash,Travis White,Maria Jasin作者: 終止 時(shí)間: 2025-3-23 00:55 作者: minimal 時(shí)間: 2025-3-23 05:23
Anastasiya Epshtein,Lorraine S. Symington,Hannah L. Klein作者: 事情 時(shí)間: 2025-3-23 07:25
Lydia R. Heasley,Nadia M. V. Sampaio,Juan Lucas Argueso作者: Infusion 時(shí)間: 2025-3-23 12:35 作者: 薄膜 時(shí)間: 2025-3-23 16:17 作者: Innocence 時(shí)間: 2025-3-23 19:29 作者: 洞察力 時(shí)間: 2025-3-23 22:34
Nancy Wong,Sam John,André Nussenzweig,Andrés Canelaauch, da? neben der Darstellung der neuen Technologien selbst eine zumindest skizzenhafte Betrachtung eben dieser r?umlichen und st?dtebaulichen Trends bzw. ihrer Bestimmungsfaktoren vonn?ten erscheint. Die Problematik verlangt darüber hinaus jedoch eine etwas ausführlichere thematische Begründung.作者: 大罵 時(shí)間: 2025-3-24 02:48 作者: Campaign 時(shí)間: 2025-3-24 09:45 作者: eczema 時(shí)間: 2025-3-24 13:21 作者: Defense 時(shí)間: 2025-3-24 17:39
END-seq: An Unbiased, High-Resolution, and Genome-Wide Approach to Map DNA Double-Strand Breaks andunrepaired, these DSBs can lead to genome instability which serves as a major driver to tumorigenesis and other pathologies. Consequently, localizing DSBs and understanding the dynamics of break formation and the repair process are of great interest for dissecting underlying mechanisms and in the de作者: 侵蝕 時(shí)間: 2025-3-24 23:03 作者: 厭惡 時(shí)間: 2025-3-25 00:41 作者: 鎮(zhèn)痛劑 時(shí)間: 2025-3-25 07:16 作者: 放肆的你 時(shí)間: 2025-3-25 08:49
Biochemical Analysis of D-Loop Extension and DNA Strand Displacement Synthesis, forks and eroded telomeres are restored. The central step in homologous recombination is homology-dependent pairing between a single-stranded DNA tail with an intact duplex molecule to generate a displacement-loop (D-loop), followed by DNA synthesis within the D-loop platform. This chapter describe作者: Occlusion 時(shí)間: 2025-3-25 11:46 作者: Resistance 時(shí)間: 2025-3-25 18:22
Monitoring Homologous Recombination Activity in Human Cells,(HR), operating only in S/G2 cell-cycle phases and nonhomologous end joining (NHEJ) which is operative throughout the cell cycle. Because HR is a template-directed repair, it is generally less prone to errors and/or translocations than NHEJ..The HR pathway involves several effector proteins and regu作者: 不適 時(shí)間: 2025-3-25 23:24 作者: Culmination 時(shí)間: 2025-3-26 01:45
Branch Migration Activity of Rad54 Protein,ctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they 作者: 大量 時(shí)間: 2025-3-26 05:49
Holliday Junction Resolution,he reciprocal exchange of single strands between two DNA molecules. Given their ability to effectively bridge two sister chromatids or homologous chromosomes, cells have implemented various pathways to ensure their timely removal. One of them is the nucleolytic processing of the Holliday junctions b作者: somnambulism 時(shí)間: 2025-3-26 12:31 作者: 煩躁的女人 時(shí)間: 2025-3-26 14:47
Intrachromosomal Recombination in Yeast,regions of the genome that are not repetitive, spontaneous recombination rates are too low to be detected by simple screening and require reporters where a recombination product can be selected. This chapter describes commonly used types of reporters where a gene is duplicated as direct repeats and 作者: inundate 時(shí)間: 2025-3-26 17:37
Genome-Wide Analysis of Mitotic Recombination in Budding Yeast,athway that guides the repair reaction by using a homologous DNA template. The budding yeast . is an excellent model system with which to study this repair mechanism and the resulting patterns of genomic change resulting from it. In this chapter, we describe an approach that utilizes whole-genome se作者: Feckless 時(shí)間: 2025-3-26 22:19 作者: OTTER 時(shí)間: 2025-3-27 04:53
DNA Double-Strand Break-Induced Gene Amplification in Yeast, optimize production of proteins and metabolites. Currently, available methods can provide high gene-expression levels but fail to achieve accurate gene dosage. Moreover, strains generated using these methods often suffer from genetic instability resulting in loss of gene copies during prolonged cul作者: 南極 時(shí)間: 2025-3-27 08:39
Measuring Chromosome Pairing During Homologous Recombination in Yeast,r. Here we describe a method to measure pairing of DNA loci during homologous recombination (HR) at a site-specific double-strand break (DSB) in .. This method utilizes a chromosome tagging system in diploid yeast cells to visualize both the DNA at the break site and the homologous DNA that serves a作者: ANTE 時(shí)間: 2025-3-27 10:36
Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes,ociated with infertility, pregnancy miscarriage, and congenital disease. This chapter presents optimized protocols for the analysis of meiotic crossovers at the cytological level in spermatocytes and oocytes from mouse. The first approach employs immunocytology to detect MLH1, a DNA mismatch-repair 作者: Grating 時(shí)間: 2025-3-27 17:20
r fest: ?Das Gespenst der technologischen Arbeitslosigkeit ... ist wieder da. Aber es gibt gute Gründe zu glauben, da? es sich diesmal nicht zurückziehen wird“. (Leontief 1983). In einer Studie über die Auswirkungen der Automation auf die Arbeitskr?fte, die von Leontief und seinen Mitarbeitern in eb作者: 礦石 時(shí)間: 2025-3-27 19:50
Sonia I. Barroso,Andrés Aguileraneue Entwicklungen hervorgehoben, die als Umbruch in der betrieblichen Rationalisierungsstrategie interpretiert werden. Für einen solchen Umbruch stehen etwa die ?Neuen Produktionskonzepte“ (Kern/Schumann 1984), der ?Neue Rationalisierungstyp“ (Altmann u.a. 1986) oder die ?Systemische Rationalisieru作者: analogous 時(shí)間: 2025-3-27 23:51 作者: ALE 時(shí)間: 2025-3-28 04:28
Erika Casari,Elisa Gobbini,Michela Clerici,Maria Pia Longhese und somit auch zu einer erh?hten Inanspruchnahme von Leistungen des Gesundheitswesens. Die Bev?lkerungsgr??e und -entwicklung ist die wohl wichtigste Einflu?gr??e auf die Morbidit?t einer Bev?lkerung. Der Bedeutung der Entwicklung einer Bev?lkerung und ihrer Gr??e auf den Gesundheitszustand wird du作者: 個(gè)阿姨勾引你 時(shí)間: 2025-3-28 06:36 作者: 招致 時(shí)間: 2025-3-28 12:36
Yan Yun,Masaru Ito,Sumit Sandhu,Neil Huntertlicher, zivilrechtlicher und ?ffentlich-rechtlicher VorschrDieses Handbuch beleuchtet den Internetversandhandel mit illegalen Arzneimitteln über illegale Online-?Apotheken“, der sich zu einem auff?lligen Wachstumsmarkt entwickelt hat. Durch die hohen Gewinnmargen und das niedrige Entdeckungsrisiko 作者: Harbor 時(shí)間: 2025-3-28 16:43 作者: Highbrow 時(shí)間: 2025-3-28 20:07 作者: echnic 時(shí)間: 2025-3-29 00:22
Holliday Junction Resolution,ranslational modifications like phosphorylation. Here, we describe a protocol to purify . Yen1 resolvase in two different phosphorylation states (high and low) and to set up a biochemical assay to compare their ability to process a synthetic, oligonucleotide-based Holliday junction structures.作者: Dictation 時(shí)間: 2025-3-29 06:04
Genome-Wide Analysis of Mitotic Recombination in Budding Yeast, with limited experience in computational methods to conceptually understand and execute the steps of genome-wide LOH analysis as well as to adapt and apply them to their own specific studies and experimental models.作者: 呼吸 時(shí)間: 2025-3-29 10:00 作者: 含糊其辭 時(shí)間: 2025-3-29 13:08 作者: interrogate 時(shí)間: 2025-3-29 17:27 作者: HALO 時(shí)間: 2025-3-29 21:47
Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in ,dditional assay to follow resection involves recombination between two direct repeats by single-strand annealing (SSA), since SSA requires extensive resection to expose two single-strand repeats for annealing. The kinetics of resection and SSA repair are then measured using Southern blots.作者: forthy 時(shí)間: 2025-3-30 02:31
Biochemical Analysis of D-Loop Extension and DNA Strand Displacement Synthesis,s biochemical assays for (1) D-loop formation and DNA synthesis within the D-loop and (2) DNA strand displacement synthesis to test the role of DNA helicases (e.g., Pif1) in repair DNA synthesis. These mechanistic assays are valuable for elucidating the molecular details of HR.作者: 無(wú)目標(biāo) 時(shí)間: 2025-3-30 07:11 作者: 名字 時(shí)間: 2025-3-30 11:12 作者: Anthrp 時(shí)間: 2025-3-30 12:52 作者: accordance 時(shí)間: 2025-3-30 16:57
,Detection of DNA Double-Strand Breaks by γ-H2AX Immunodetection,tion of DSBs with different diseases and its potential to kill cells. DSB levels can be obtained by measuring levels of γ-H2AX in extracts of cell populations or by counting foci in individual nuclei. In this chapter some techniques to detect γ-H2AX are described.作者: 別炫耀 時(shí)間: 2025-3-30 23:06
Quantifying DNA End Resection in Human Cells,thods. This assay, excluding the time for making the stable cell line expressing the restriction enzyme .SI fused to the estrogen receptor hormone-binding domain (ER-.SI), can be completed within 3?days.作者: avarice 時(shí)間: 2025-3-31 03:38
Intrachromosomal Recombination in Yeast,n the two defective copies can result in a wild-type gene and a prototrophic phenotype for the cell. Methods to use these types of reporters to determine recombination rates between the two gene copies are described, and their use in monitoring both increased and decreased recombinations is discussed.作者: paragon 時(shí)間: 2025-3-31 07:34 作者: Aboveboard 時(shí)間: 2025-3-31 09:48 作者: ALOFT 時(shí)間: 2025-3-31 13:53
Branch Migration Activity of Rad54 Protein,ctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.作者: 減至最低 時(shí)間: 2025-3-31 17:38
Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis,logous recombination. A method is described using an ectopic homologous donor sequence to repair an HO endonuclease-induced DSB. These gene conversion events can occur with or without crossing-over, the products of which are distinguished as different-sized restriction endonuclease fragments. The method of Southern blotting is described in detail.作者: 過(guò)于平凡 時(shí)間: 2025-3-31 22:32 作者: 不斷的變動(dòng) 時(shí)間: 2025-4-1 02:59
978-1-0716-0646-9Springer Science+Business Media, LLC, part of Springer Nature 2021作者: emission 時(shí)間: 2025-4-1 07:55 作者: 改正 時(shí)間: 2025-4-1 11:14
