標(biāo)題: Titlebook: Histones; Methods and Protocol Benoit Guillemette,Luc R. Gaudreau Book 2017 Springer Science+Business Media New York 2017 eukaryotic cells. [打印本頁] 作者: 喜悅 時(shí)間: 2025-3-21 17:14
書目名稱Histones影響因子(影響力)
書目名稱Histones影響因子(影響力)學(xué)科排名
書目名稱Histones網(wǎng)絡(luò)公開度
書目名稱Histones網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Histones被引頻次
書目名稱Histones被引頻次學(xué)科排名
書目名稱Histones年度引用
書目名稱Histones年度引用學(xué)科排名
書目名稱Histones讀者反饋
書目名稱Histones讀者反饋學(xué)科排名
作者: 赤字 時(shí)間: 2025-3-22 00:03
Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLChigh-performance liquid chromatography. This method allows the preparation of micrograms to milligram amounts of in vitro-assembled nucleosomes. Finally, we demonstrate the efficiency of this method for the structural analysis of nucleosomes in the analytical ultracentrifuge.作者: Insufficient 時(shí)間: 2025-3-22 03:34 作者: 徹底檢查 時(shí)間: 2025-3-22 06:12 作者: 細(xì)節(jié) 時(shí)間: 2025-3-22 12:48 作者: armistice 時(shí)間: 2025-3-22 13:00
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro,oth in vivo and in vitro. Here, we describe an assay to measure the histone H2A.Z exchange activity of recombinant human p400 on immobilized mononucleosomes in vitro. The assay can be adapted to other histone exchange complexes/catalytic subunits purified from any species.作者: hermetic 時(shí)間: 2025-3-22 19:14 作者: Panacea 時(shí)間: 2025-3-22 22:08 作者: LITHE 時(shí)間: 2025-3-23 01:52
Immunofluorescence of Histone Proteins,nformation can be gathered from indirect immunofluorescence, where the location and cell cycle relationships can be readily identified. Here we present an immunofluorescence protocol that is adapted for the use in histone modifications.作者: 推遲 時(shí)間: 2025-3-23 05:57 作者: Canopy 時(shí)間: 2025-3-23 12:33
Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Compl for purification of native chromatin from yeast cells (Chapter .). Here, we present the methods to obtain highly purified native chromatin modifying complexes from ., based on Tandem Affinity Purification (TAP). We also present possible applications and useful functional assays that can be performed using these yeast native reagents.作者: ALIAS 時(shí)間: 2025-3-23 17:03
Production and Purification of Antibodies Against Histone Modifications,ions of canonical histones. Our protocols can be applied to obtain antibodies that occur in histone variants and proteins other than histones. In addition, some of our protocols are compatible with the production of monoclonal or recombinant antibodies.作者: angina-pectoris 時(shí)間: 2025-3-23 18:52
A Method for Large-Scale Screening of Random Sequence Libraries to Determine the Function of Unstruhod typically identifies sequences that, while being totally different from the wild type, retain essential features allowing yeast to live. This collection of proteins with functional synthetic sequences can subsequently be used in phenotypic tests or genetic screens in order to discover genetic interaction.作者: 光滑 時(shí)間: 2025-3-23 23:58 作者: promote 時(shí)間: 2025-3-24 04:47
Histone Purification from ,,osttranslational modifications that regulate nucleosome interactions, position, and stability..We describe a protocol allowing the robust purification of histones from the yeast .. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.作者: 注入 時(shí)間: 2025-3-24 08:35
Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast,ing sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns both at individual loci and genome-wide. Here we provide a detailed protocol for ChIP of histone modifications associated with active transcription in fission yeast (.).作者: 發(fā)出眩目光芒 時(shí)間: 2025-3-24 11:46 作者: 喃喃訴苦 時(shí)間: 2025-3-24 16:22 作者: 潛移默化 時(shí)間: 2025-3-24 20:55 作者: ethnology 時(shí)間: 2025-3-25 01:22
978-1-4939-8254-7Springer Science+Business Media New York 2017作者: Psa617 時(shí)間: 2025-3-25 07:20 作者: 文件夾 時(shí)間: 2025-3-25 07:58 作者: 相信 時(shí)間: 2025-3-25 12:30
Arnob Dutta,Jerry L. Workmane intraplate earthquakes during 1913–1988. These numbers do not include earthquake swarms, which account for another 838 events. Most of the remainder (304 events) are actually plate boundary earthquakes that have been erroneously located in intraplate regions. A significant number occur in recent y作者: Carcinoma 時(shí)間: 2025-3-25 16:54 作者: infinite 時(shí)間: 2025-3-25 20:08 作者: OGLE 時(shí)間: 2025-3-26 01:18 作者: 發(fā)炎 時(shí)間: 2025-3-26 06:07
Frederic Jourquin,Vincent Géliconomic and political development of the country. While it is generally believed that a dichotomy exists between politics and religion, the situation on the ground shows that there is no separation. This study examines the history of the relationship between Pentecostal churches and politics before 作者: Affection 時(shí)間: 2025-3-26 09:11 作者: fender 時(shí)間: 2025-3-26 12:51
Alessandro Cuomo,Monica Soldi,Tiziana Bonaldi Ph.D.the syntactic structure of the sentence in a direct way. In contrast, semantic interpretations of sentences of natural languages, for example, English, have, in transformational grammars, taken place on translations of those sentences into some language similar to the languages of formal logic. It i作者: 起波瀾 時(shí)間: 2025-3-26 19:20
Simone Sidoli,Benjamin A. Garcia the 3rd of December 1977. Its main body consists of revised versions of most of the papers presented on that occasion. Three supplementary papers (those by Gabbay and Sma by) are included because they seem to be of particular interest in their respective fields. The area covered by the work of scho作者: 尖牙 時(shí)間: 2025-3-26 22:43 作者: bronchiole 時(shí)間: 2025-3-27 01:14
Hilmar Strickfaden,Michael J. Hendzele functioning of a single neuron are quite well understood, the collective behavior of a system of many neurons is an extremely intriguing subject. Crucial ingredient of this complex behavior is the plasticity property of the network, namely the capacity to adapt and evolve depending on the level of作者: 浮雕 時(shí)間: 2025-3-27 07:56
Catherine A. Hazzalin,Louis C. Mahadevan hydrophobic force, the water-mediated interaction deemed to provide an impetus for the organization of living matter. Hydrophobic forces can be traced back to the dynamic and structural changes undergone by water at biological surfaces. Since biological surfaces are quite heterogeneous with regard 作者: 飛行員 時(shí)間: 2025-3-27 10:16
Jean Mbogning,Jason C. Tannyo the emergence of a new interdisciplinary field, called Physical Biology, with the aim of reaching a better understanding of the biological mechanisms at molecular and cellular levels. Statistical Mechanics in particular plays an important role in the development of this new field...For this reason作者: 熒光 時(shí)間: 2025-3-27 16:51 作者: pessimism 時(shí)間: 2025-3-27 18:21
Mylène Brunelle,Sébastien Rodrigue,Pierre-étienne Jacques,Nicolas Gévryt each daughter cell contains a complete set of genes. The duplication process is called DNA replication and it starts at one defined site on the DNA molecule called the origin of replication (.) [1]. In addition to chromosomal DNA, bacteria often also contain plasmid DNA. Plasmids are extra-chromos作者: Engulf 時(shí)間: 2025-3-27 22:39
Vanessa Menil-Philippot,Christophe Thiriet week of gestation. A functioning foeto-placental unit is necessary for most of the increased oestrogen production of pregnancy and for the provision of glucocorticoids and aldosterone to the foetus. Neither the foetus nor the placenta alone have the necessary enzyme systems for the synthesis of the作者: 采納 時(shí)間: 2025-3-28 05:40 作者: 會(huì)犯錯(cuò)誤 時(shí)間: 2025-3-28 09:45
week of gestation. A functioning foeto-placental unit is necessary for most of the increased oestrogen production of pregnancy and for the provision of glucocorticoids and aldosterone to the foetus. Neither the foetus nor the placenta alone have the necessary enzyme systems for the synthesis of the作者: inspiration 時(shí)間: 2025-3-28 13:00
In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays, templates. In this chapter, we describe procedures for purification of histones from ., formation of octamers, and reconstitution of nucleosomes that can be further modified by chromatin modifiers. In addition, we describe methods to purify nucleosomes from human cells. Finally, we also describe as作者: forebear 時(shí)間: 2025-3-28 17:10
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro,One such example is H2A.Z, a highly conserved variant of H2A that is incorporated in genomic regulatory regions and contributes to control gene expression. H2A.Z variant exchange involves the removal of H2A-H2B dimers from a preassembled nucleosome and their replacement with H2A.Z-H2B dimers. A spec作者: bonnet 時(shí)間: 2025-3-28 22:40
Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Recstone proteins harbor numerous post-translational modifications, which may differ between species, tissues, and growth conditions and are lacking on recombinant histones. Moreover, the physiological substrate of most enzymes that modify histones is chromatin and the majority of these enzymes need to作者: Hangar 時(shí)間: 2025-3-29 02:07
Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Compland there is an intricate cross-talk between different marks as they are recognized by specific reader modules present in a large number of nuclear factors. In order to precisely dissect these processes in vitro native reagents like purified chromatin and histone modifying/remodeling enzymes are req作者: 惡意 時(shí)間: 2025-3-29 06:24 作者: Benzodiazepines 時(shí)間: 2025-3-29 09:44
Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLCetics demand the availability of methods for a rapid and efficient nucleosome reconstitution to analyze their structural and functional implications. Here we describe a method suitable for this purpose, starting from bacterially expressed histones, solubilized by acid and purified by reversed-phase 作者: Glycogen 時(shí)間: 2025-3-29 13:01
SILAC-Based Quantitative Strategies for Accurate Histone Posttranslational Modification Profiling Ay (MS) has emerged as a versatile technology for the analysis of histones, contributing to the dissection of hPTMs, with special strength in the identification of novel marks and in the assessment of modification cross talks. Stable isotope labeling by amino acid in cell culture (SILAC), when adapte作者: 新娘 時(shí)間: 2025-3-29 19:25 作者: installment 時(shí)間: 2025-3-29 21:24
Production and Purification of Antibodies Against Histone Modifications,ly available antibodies that recognize a remarkable diversity of histone modifications. Unfortunately, many of them fail to work in certain applications or lack the high degree of specificity required of these reagents. The production of affinity-purified polyclonal antibodies against histone modifi作者: Substitution 時(shí)間: 2025-3-30 03:28 作者: 榨取 時(shí)間: 2025-3-30 08:07 作者: magnate 時(shí)間: 2025-3-30 10:39 作者: 凹室 時(shí)間: 2025-3-30 13:05
A Spiking Strategy for ChIP-chip Data Normalization in ,,iants or histone modifications along the genome. Often, the position and the occupancy of these epigenetic marks are to be compared between different experiments. It is now increasingly recognized that such cross-sample comparison is better done using externally added exogenous controls for normaliz作者: 徹底檢查 時(shí)間: 2025-3-30 16:58 作者: MILK 時(shí)間: 2025-3-30 20:46 作者: thalamus 時(shí)間: 2025-3-31 03:55
A Method for Large-Scale Screening of Random Sequence Libraries to Determine the Function of Unstru The approach can be applied to any protein with unstructured peptide sequences for which functions are difficult to decipher, for example the N-terminal tails of histones. The protocol first describes the building and preparation of a large library of random peptides in fusion with a protein of int作者: 疾馳 時(shí)間: 2025-3-31 07:18
