標(biāo)題: Titlebook: High-Resolution Imaging of Cellular Proteins; Methods and Protocol Steven D. Schwartzbach,Omar Skalli,Thomas Schikors Book 2016 Springer Sc [打印本頁] 作者: 門牙 時間: 2025-3-21 16:35
書目名稱High-Resolution Imaging of Cellular Proteins影響因子(影響力)
書目名稱High-Resolution Imaging of Cellular Proteins影響因子(影響力)學(xué)科排名
書目名稱High-Resolution Imaging of Cellular Proteins網(wǎng)絡(luò)公開度
書目名稱High-Resolution Imaging of Cellular Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱High-Resolution Imaging of Cellular Proteins被引頻次
書目名稱High-Resolution Imaging of Cellular Proteins被引頻次學(xué)科排名
書目名稱High-Resolution Imaging of Cellular Proteins年度引用
書目名稱High-Resolution Imaging of Cellular Proteins年度引用學(xué)科排名
書目名稱High-Resolution Imaging of Cellular Proteins讀者反饋
書目名稱High-Resolution Imaging of Cellular Proteins讀者反饋學(xué)科排名
作者: 狂熱語言 時間: 2025-3-21 21:13 作者: Friction 時間: 2025-3-22 02:20 作者: Oscillate 時間: 2025-3-22 06:39 作者: 陶器 時間: 2025-3-22 09:05 作者: Eulogy 時間: 2025-3-22 15:51
hlich war das Bestreben, die Bewegung von K?rpern zu beschreiben, vor mehr als 400 Jahren die Geburtsstunde der Physik..Die Bewegung und die damit zusammenh?ngenden Konzepte der Kraft und der Masse bilden den Gegenstand der Mechanik. Im Rahmen der Mechanik werden wir uns zun?chst der Kinematik zuwen作者: commodity 時間: 2025-3-22 19:43
Harumi Harada,Ryuichi Shigemotontalphysik.Erweitert und verbessert durch Einbezug moderner .Das Arbeitsbuch zu ?Physik - für Studierende der Naturwissenschaften und Technik“ von Paul A. Tipler und Gene Mosca enth?lt alle Aufgaben der achten deutschsprachigen Ausgabe sowie deren ausführliche L?sungen..Mit über 1200 Aufgaben - daru作者: 流逝 時間: 2025-3-22 23:53
Expression of Epitope-Tagged Proteins in Mammalian Cells in Culturetein of interest. Thus, only proteins for which antibodies were available could be visualized. Epitope tagging allows the detection of all proteins with existing sequence information, irrespective of the availability of antibodies directed against them. This technique involves the generation of DNA 作者: AIL 時間: 2025-3-23 02:40
Antibody Production with Synthetic Peptidesclonal antibodies routinely with titers higher than 20,000. Peptide antigens do not function as immunogens unless they are conjugated to proteins. Production of high quality antipeptide antibodies is dependent upon peptide sequence selection, the success of peptide synthesis, peptide–carrier protein作者: canvass 時間: 2025-3-23 06:23
Production and Purification of Polyclonal Antibodieslls in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclon作者: pulmonary-edema 時間: 2025-3-23 11:45
Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab′)2, and Streptavells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.作者: 并入 時間: 2025-3-23 17:02 作者: jettison 時間: 2025-3-23 19:57 作者: 楓樹 時間: 2025-3-23 22:43 作者: fulcrum 時間: 2025-3-24 04:37 作者: Monotonous 時間: 2025-3-24 09:48
Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imagingn vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applica作者: 健壯 時間: 2025-3-24 13:38 作者: 平 時間: 2025-3-24 15:50 作者: WATER 時間: 2025-3-24 19:12 作者: DEVIL 時間: 2025-3-25 02:34
Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissuestructural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more 作者: 黑豹 時間: 2025-3-25 05:15
Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast ,g offers an excellent way to fix cellular structure. However, its use for immunolabeling has remained limited because of the low frequency of labeling due to loss of protein antigenicity or accessibility. Here we present a protocol for immunogold labeling of the yeast . that gives specific and multi作者: Hiatus 時間: 2025-3-25 09:00
Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Ug systems using lectin microarrays, and glycoprotein analysis by the isotope-coded glycosylation site-specific tagging method. With these methodologies, glycan structures and biological functions are being elucidated. In the study of glycan function as well as disease diagnosis, it is important to e作者: 殺菌劑 時間: 2025-3-25 14:35 作者: Robust 時間: 2025-3-25 17:01 作者: GUILT 時間: 2025-3-25 22:09 作者: Indict 時間: 2025-3-26 02:53 作者: Eviction 時間: 2025-3-26 04:17 作者: agenda 時間: 2025-3-26 09:29
Production and Purification of Polyclonal Antibodieslls in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.作者: 情感 時間: 2025-3-26 16:00
Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab′)2, and Streptavells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.作者: voluble 時間: 2025-3-26 19:54
Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interactions and Assessmn example to provide a step-by-step BiFC protocol using a Nikon A1 confocal microscope and NIS-Elements imaging software. The protocol given below can be readily adapted for use with other confocal microscopes or imaging software.作者: 偽造 時間: 2025-3-27 00:37
Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteinsc vesicle endocytosis in response to extracellular stimulation in dissociated neuronal cultures of hippocampal neurons obtained from rats—also applicable to mice—using pHluorin-tagged vesicular glutamate transporter-1 as a reporter.作者: Cardioplegia 時間: 2025-3-27 01:26
Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cellsg embedded in an epoxy resin for ultrathin sectioning. The protocol also includes strategies for optimizing the balance between ultrastructure and antigen preservation, steps to minimize nonspecific antibody binding, and steps to optimize antibody penetration.作者: 防止 時間: 2025-3-27 07:59
1064-3745 ation advice from the experts.Includes supplementary materia.This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron 作者: 防止 時間: 2025-3-27 12:21 作者: handle 時間: 2025-3-27 17:20 作者: LASH 時間: 2025-3-27 20:46
Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organellactive transport. These two techniques have been extensively utilized to probe the cell biology of neurons. A practical outline of FRAP and FRAPA in cultured neurons is presented, including the preparation of the neurons and their infection with adeno-associated viral vectors. Considerations in planning such experiments are provided.作者: 減震 時間: 2025-3-27 23:50 作者: rheumatism 時間: 2025-3-28 05:00 作者: 粗鄙的人 時間: 2025-3-28 07:42 作者: Ventilator 時間: 2025-3-28 10:25
Book 2016s used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluoresc作者: unstable-angina 時間: 2025-3-28 15:50 作者: 搖晃 時間: 2025-3-28 22:27 作者: ARY 時間: 2025-3-28 23:38
Localizing Proteins in Fixed , and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy作者: 光明正大 時間: 2025-3-29 05:11 作者: HUMP 時間: 2025-3-29 09:42
Yaara Tevet,Daniel Gitlere zur Bearbeitung der Kleinstlernfragen 3. Kleinstlernfragen zum Ersten Kapitel I 3 16 4. Wichtige Hinweise zur Bearbeitung der Lernfragen 5. Lernfragen ????????? 17 26 6. Wichtige Hinweise zur Bearbeitung der Kontrollfragen und Aufgaben ????? 7. Kontrollfragen mit L?sungen . . . . . . . . . . . . .作者: hurricane 時間: 2025-3-29 13:55
Evan P. S. Pratt,Jake L. Owens,Gregory H. Hockerman,Chang-Deng Huo bleibt ein gro?er Teil der kinetischen Energie aller Sto?partner erhalten. W?hrend des kurzen Moments, in dem die Billardkugeln aufeinandertreffen, wird die kinetische Energie kurzzeitig in Form einer elastischen Deformation gespeichert. Sto?prozesse, die so verlaufen, bezeichnet man daher auch al作者: 橫條 時間: 2025-3-29 17:29 作者: 中世紀(jì) 時間: 2025-3-29 20:19 作者: 預(yù)感 時間: 2025-3-30 02:23 作者: chisel 時間: 2025-3-30 04:43
Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture in cells provide different types of information depending on the subsequent detection approaches. Using immunofluorescence and immunoelectron microscopy with anti-tag antibodies, relative to known markers of cellular organelles, can provide information on the subcellular localization of the tagged 作者: Hemiplegia 時間: 2025-3-30 10:59 作者: 旁觀者 時間: 2025-3-30 13:55 作者: intimate 時間: 2025-3-30 17:31 作者: 尖酸一點(diǎn) 時間: 2025-3-30 21:28
Harumi Harada,Ryuichi Shigemotochenphysik..Studierende k?nnen hier physikalisches Probleml?sen mit Blick auf klassische Standardexperimente, aber auch?moderne Anwendungen und aktuelle Entwicklungenüben und erlernen – und zwar mit Spa? und Er978-3-662-58918-2978-3-662-58919-9作者: 鋼盔 時間: 2025-3-31 01:49 作者: 漫步 時間: 2025-3-31 06:19
Bao-Shiang Lee,Jin-Sheng Huang,Lasanthi P. Jayathilaka,Jenny Lee,Shalini Gupta.作者: sigmoid-colon 時間: 2025-3-31 09:57
