標(biāo)題: Titlebook: Heterologous Protein Production in CHO Cells; Methods and Protocol Paula Meleady Book 2017 Springer Science+Business Media LLC 2017 CRISPR. [打印本頁] 作者: MIFF 時(shí)間: 2025-3-21 16:03
書目名稱Heterologous Protein Production in CHO Cells影響因子(影響力)
書目名稱Heterologous Protein Production in CHO Cells影響因子(影響力)學(xué)科排名
書目名稱Heterologous Protein Production in CHO Cells網(wǎng)絡(luò)公開度
書目名稱Heterologous Protein Production in CHO Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Heterologous Protein Production in CHO Cells被引頻次
書目名稱Heterologous Protein Production in CHO Cells被引頻次學(xué)科排名
書目名稱Heterologous Protein Production in CHO Cells年度引用
書目名稱Heterologous Protein Production in CHO Cells年度引用學(xué)科排名
書目名稱Heterologous Protein Production in CHO Cells讀者反饋
書目名稱Heterologous Protein Production in CHO Cells讀者反饋學(xué)科排名
作者: Heretical 時(shí)間: 2025-3-21 23:41
Heterologous Protein Production in CHO Cells978-1-4939-6972-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Myosin 時(shí)間: 2025-3-22 03:28 作者: Chauvinistic 時(shí)間: 2025-3-22 06:35
Paula MeleadyIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: SOB 時(shí)間: 2025-3-22 11:35 作者: brachial-plexus 時(shí)間: 2025-3-22 15:19 作者: AVID 時(shí)間: 2025-3-22 17:52
Eric Baek,Soo Min Noh,Gyun Min Leeactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: 領(lǐng)袖氣質(zhì) 時(shí)間: 2025-3-23 00:07
Zeynep Betts Ph.D.,Alan J. Dicksonactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: 中子 時(shí)間: 2025-3-23 04:40
Clair Gallagher,Paul S. Kellyactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: LAST 時(shí)間: 2025-3-23 06:06 作者: MAZE 時(shí)間: 2025-3-23 11:57
Orla Coleman,Michael Henry,Martin Clynes,Paula Meleadyactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: liaison 時(shí)間: 2025-3-23 14:50
Yuzhou Fan,Helene Faustrup Kildegaard,Mikael R?rdam Andersenactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: 偽善 時(shí)間: 2025-3-23 20:38
Sara Carillo,Stefan Mittermayr,Amy Farrell,Simone Albrecht,Jonathan Bonesactice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: 合唱團(tuán) 時(shí)間: 2025-3-24 01:05
actice in one comprehensive reference book. The chapters are written by well recognized experts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..作者: Mortar 時(shí)間: 2025-3-24 02:44 作者: Exaggerate 時(shí)間: 2025-3-24 09:59 作者: 法官 時(shí)間: 2025-3-24 10:50 作者: AER 時(shí)間: 2025-3-24 18:16 作者: 譏笑 時(shí)間: 2025-3-24 22:24
Alan Costello,Nga Lao,Martin Clynes,Niall Barronperts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..978-3-319-51907-4978-3-319-51908-1Series ISSN 2191-5709 Series E-ISSN 2191-5717 作者: 乳白光 時(shí)間: 2025-3-24 23:37
Lise Marie Grav,Karen Julie la Cour Karottki,Jae Seong Lee,Helene Faustrup Kildegaardperts in these fields. The book is addressed to everyone involved in internal medicine, anesthesia, surgery, pediatrics, intensive care and emergency medicine..978-3-319-51907-4978-3-319-51908-1Series ISSN 2191-5709 Series E-ISSN 2191-5717 作者: 沖突 時(shí)間: 2025-3-25 05:20 作者: sterilization 時(shí)間: 2025-3-25 10:43 作者: 珊瑚 時(shí)間: 2025-3-25 14:42 作者: Frisky 時(shí)間: 2025-3-25 17:54
,Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cecould result in low protein expression, while the inherent properties of the molecule itself may limit its production via mechanisms such as cytotoxicity or inherent instability. Strategies to identify the rate-limiting step and subsequently improve expression and production are discussed here.作者: EXUDE 時(shí)間: 2025-3-25 20:42
Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells,PR-associated protein 9 (Cas9) to knockout engineering target genes in CHO cells. As an example, we refer to the glutamine synthetase (GS)-encoding gene as the knockout target gene, a knockout that increases the selection efficiency of the GS-mediated gene amplification system.作者: 詞匯表 時(shí)間: 2025-3-26 02:26 作者: 債務(wù) 時(shí)間: 2025-3-26 06:01
A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-tional pipeline for the analysis of CHO cell RNA-Seq data from the Illumina platform to identify differentially expressed genes. The example data and bioinformatics workflow required to run this analysis are freely available at ..作者: entrance 時(shí)間: 2025-3-26 10:28
Book 2017s for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful .Methods作者: 不公開 時(shí)間: 2025-3-26 16:32 作者: 抵押貸款 時(shí)間: 2025-3-26 18:27 作者: 不可磨滅 時(shí)間: 2025-3-27 00:02 作者: Keratin 時(shí)間: 2025-3-27 05:05 作者: TOXIN 時(shí)間: 2025-3-27 07:51 作者: 迫擊炮 時(shí)間: 2025-3-27 12:51
Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expretions and assembled by a single overlap-extension PCR reaction. The amplified product is then cloned into a mammalian expression vector suitable for high-titer transient gene expression. This workflow can be applied to any antibody sequence by adapting the specific primer sequences to the antibody of choice.作者: 薄荷醇 時(shí)間: 2025-3-27 17:14
Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture,e stability of the recombinant CHO cells over long-term culture. These procedures include the following; western blotting, ELISA to evaluate protein production, real-time PCR to analyze plasmid and mRNA copy numbers, and fluorescent in situ hybridization (FISH) to assess the location of the inserted plasmid on host cell chromosomes.作者: BYRE 時(shí)間: 2025-3-27 19:20 作者: 6Applepolish 時(shí)間: 2025-3-27 23:28
Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension,incubation at 31 °C with agitation by orbital shaking. We also describe an alternative method in which 90% of the pDNA is replaced by nonspecific (filler) DNA, and the production phase is performed at 31 °C in the presence of 0.25% N, N-dimethylacetamide (DMA).作者: travail 時(shí)間: 2025-3-28 05:33 作者: 歌劇等 時(shí)間: 2025-3-28 09:03
Selection of High-Producing Clones Using FACS for CHO Cell Line Development,powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.作者: inquisitive 時(shí)間: 2025-3-28 13:04
Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses, while estimation of HCP clearance/presence can be achieved by comparing 2D-PAGE images of samples and by undertaking western blotting of 2D-PAGE analyzed samples. Here, we describe the analyses of HCP content using these methodologies.作者: 威脅你 時(shí)間: 2025-3-28 17:18
,Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Ceg the expression bottleneck requires troubleshooting techniques specific for the given molecule and host. The complex array of intracellular processes involved in protein expression includes transcription, protein folding, post-translation processing, and secretion. Challenges in any of these steps 作者: sigmoid-colon 時(shí)間: 2025-3-28 18:45
Glycoengineering of CHO Cells to Improve Product Quality,oteins, especially glycoproteins. These glycoproteins include oligosaccharide or glycan attachments that represent one of the principal components dictating product quality. Especially important are the N-glycan attachments present on many recombinant glycoproteins of commercial interest. Furthermor作者: languid 時(shí)間: 2025-3-29 01:40 作者: Custodian 時(shí)間: 2025-3-29 06:47 作者: Outspoken 時(shí)間: 2025-3-29 11:07
Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells,t production of therapeutic proteins. By establishing stable cell lines that overexpress anti-apoptotic genes or down-regulate pro-apoptotic genes, the final product yields can be enhanced as cells become more resistance to environmental stresses. From the selection of high-expressing clones to veri作者: 使習(xí)慣于 時(shí)間: 2025-3-29 12:02
Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-, Sponge Vectors,rable phenotype in the biotech industry. The use of constitutively expressed “miRNA sponge” vectors in which multiple, tandem miRNA binding sites containing transcripts are transcriptionally regulated by a constitutive promoter for down regulating the levels of endogenous microRNAs in Chinese hamste作者: 故意 時(shí)間: 2025-3-29 16:01 作者: 中子 時(shí)間: 2025-3-29 21:16 作者: 災(zāi)難 時(shí)間: 2025-3-30 03:04 作者: MUMP 時(shí)間: 2025-3-30 05:13 作者: invulnerable 時(shí)間: 2025-3-30 10:43
A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA- biopharmaceutical cell factories with unprecedented resolution. Our understanding of the CHO cell transcriptome, in particular, has rapidly advanced through the application of next-generation sequencing (NGS) technology to characterize RNA expression (RNA-Seq). In this chapter, we present a computa作者: harangue 時(shí)間: 2025-3-30 13:29 作者: 鐵塔等 時(shí)間: 2025-3-30 18:48 作者: breadth 時(shí)間: 2025-3-31 00:25 作者: incubus 時(shí)間: 2025-3-31 04:39
Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells,progressing through clinical trials, solidifying biologics as the most promising class of pharmaceuticals on the market. Several cell lines are available for biopharmaceutical processes but mammalian cells are preferred since they give fewer problems for immunogenicity as they produce human-like pos作者: 粉筆 時(shí)間: 2025-3-31 06:02 作者: languor 時(shí)間: 2025-3-31 11:55
