作者: 精美食品 時(shí)間: 2025-3-21 20:13 作者: 貧困 時(shí)間: 2025-3-22 03:26
Christine A. MacLeanirst sight, this may seem as though we were avoiding the central issue of the conference altogether, but we took our decision in the belief that by trying to discover the processes, cognitive or otherwise, that lie behind animal behavior, we stood the best chance of being able to generate testable h作者: 兇猛 時(shí)間: 2025-3-22 05:31
Alasdair R. MacLean can be trained to follow the commands of their masters, only humans seem to be capable of learning to the extent that they engage in arts and sciences. Nevertheless, certain nonhuman animals, namely, monkeys and so-called pygmies come very close to humans because of their faculty of estimation and 作者: Celiac-Plexus 時(shí)間: 2025-3-22 11:28
Robert S. Coffin theory he notes that it gives rise to various problems and questions. He wonders, for example, whether intentions are universals, that is, general concepts. They seem to be universal insofar as, for instance, the feature of hostility is found in all wolves. Hence, the question arises whether nonhum作者: Ingratiate 時(shí)間: 2025-3-22 16:32 作者: 冥想后 時(shí)間: 2025-3-22 17:10 作者: Concomitant 時(shí)間: 2025-3-22 23:24
Lars Haarr,Nina Langeland inflammation.Details methods for the functional, morphologi.Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our 作者: peak-flow 時(shí)間: 2025-3-23 01:36
Joseph Conner, and appropriate study models, especially animal models, are essential to furthering our understanding of the etiology, pathology, and progression of these endemic diseases. In .Animal Models for Retinal Diseases., recognized experts in the field highlight valuable techniques as well as animal mode作者: expository 時(shí)間: 2025-3-23 05:42 作者: 戰(zhàn)役 時(shí)間: 2025-3-23 11:48
Ben Luisi,Marie Anderson,Graham Hope inflammation.Details methods for the functional, morphologi.Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our 作者: 健壯 時(shí)間: 2025-3-23 15:37
Linda M. Stannard, and appropriate study models, especially animal models, are essential to furthering our understanding of the etiology, pathology, and progression of these endemic diseases. In .Animal Models for Retinal Diseases., recognized experts in the field highlight valuable techniques as well as animal mode作者: 使人入神 時(shí)間: 2025-3-23 19:05 作者: 積云 時(shí)間: 2025-3-24 02:00
Anne Phelan,J. Barklie Clements, and appropriate study models, especially animal models, are essential to furthering our understanding of the etiology, pathology, and progression of these endemic diseases. In .Animal Models for Retinal Diseases., recognized experts in the field highlight valuable techniques as well as animal mode作者: 神圣將軍 時(shí)間: 2025-3-24 05:20
Jordan G. Spivack inflammation.Details methods for the functional, morphologi.Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our 作者: 字的誤用 時(shí)間: 2025-3-24 09:19 作者: overbearing 時(shí)間: 2025-3-24 14:12 作者: Frequency 時(shí)間: 2025-3-24 17:22 作者: 剝皮 時(shí)間: 2025-3-24 21:15 作者: BOOM 時(shí)間: 2025-3-25 02:50
Terry J. Hill,Carolyn Shimeld inflammation.Details methods for the functional, morphologi.Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our 作者: 提煉 時(shí)間: 2025-3-25 05:52
HSV Growth, Preparation, and Assay,quire the virus to be grown and assayed. The following chapter is therefore seen as the fundamental first step before embarking on more intellectually and technically challenging technology. Its importance should not however be underestimated. It never fails to surprize us that people who describe t作者: Licentious 時(shí)間: 2025-3-25 08:15 作者: 有發(fā)明天才 時(shí)間: 2025-3-25 15:44 作者: 禁止,切斷 時(shí)間: 2025-3-25 19:01
HSV Mutagenesis,begin as the virus enters the cell, and continue as the decision for latency or lytic replication is made. Correctly regulated gene expression then allows herpes genes to be expressed in a temporally regulated manner and to subvert the host cell metabolism in favor of virus production. Finally, infe作者: Cumbersome 時(shí)間: 2025-3-25 20:07
Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution ofs. In this respect, mutagenesis and sequence analysis of genes encoded by the virus are of great importance. However, the herpesvirus genomes are large, with sizes ranging between 120 and over 200 kbp and encoding between 70 and 200 genes (. ref. . for a review). This large size hampers handling and作者: 蛤肉 時(shí)間: 2025-3-26 01:58
Construction and Use of Cell Lines Expressing HSV Genes, in strategies for the construction of mutant viruses. More recent applications include the propagation of replication-defective virus products with potential as viral vaccines (.,.) or as vehicles for gene therapy (.,.). The perceived requirements for such systems are low recombination frequencies 作者: Counteract 時(shí)間: 2025-3-26 07:29
Analysis of HSV Polypeptides,roduced, including a host shut off protein (.–.). The inhibitory effect of the protein obviously needs a certain period of time to be fully efficient, but some cellular proteins involved in virus replication still are produced. Examples are those interacting with the regulatory viral proteins V.65 (作者: 縱欲 時(shí)間: 2025-3-26 11:39
Protein Purification,a protein can be used to generate specific polyclonal and monoclonal antibodies for in vivo studies, in vitro the enzymic properties or interactions with nucleic acids or other proteins can be studied in detail and related to in viva function and, ultimately, the purified proteins can be used in str作者: Encapsulate 時(shí)間: 2025-3-26 13:50
Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insectonatal infections. Most adults have antibodies against the oral form of the virus HSV-1 (.), and a significant number are infected with the genital form, HSV-2. Both serotypes establish lifelong latent infections and reactivate periodically to produce recurrent disease (.). After infection, virus-en作者: ERUPT 時(shí)間: 2025-3-26 20:20
Crystallization of Macromolecules for Three-Dimensional Structure Determination,ation, and has been made possible by the combined improvement in techniques of X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and the bulk preparation of biological materials. Most crucial, however, has been the desire of the experimental biologists to follow research problems作者: 百靈鳥 時(shí)間: 2025-3-27 00:57 作者: Ancillary 時(shí)間: 2025-3-27 01:20
Expression of HSV Proteins in Bacteria,s of specific viral proteins for use in both biochemical and functional analysis, and as immunogens for antisera production. Proteins can be expressed either in the full-length native form or as fusion proteins with affinity tails.作者: Lymphocyte 時(shí)間: 2025-3-27 08:26 作者: MILL 時(shí)間: 2025-3-27 11:06
Analysis of HSV-1 Transcripts by RNA-PCR,ure of many HSV-1 RNAs can be obtained without the considerable time and effort that is required to construct and analyze cDNA libraries. Once the 5′ and 3′ ends of an RNA have been mapped precisely, the RNA nucleotide sequence can be deduced simply from the genomic DNA sequence. However, there are 作者: BILIO 時(shí)間: 2025-3-27 15:27 作者: lanugo 時(shí)間: 2025-3-27 18:59
HSV Amplicons in Gene Therapy,s, such as fibroblasts, macrophages, glia, and neurons in different organisms including rodents, monkeys, and human (refs. .–.; reviewed in ref. .). The defective viruses follow their nondefective counterparts in the ability to infect mitotic, as well as postmitotic cells. This makes them potentiall作者: HARP 時(shí)間: 2025-3-28 01:55 作者: Lobotomy 時(shí)間: 2025-3-28 03:41
HSV-Cellular Protein Interactions,diate-early (IE) genes, followed by the early genes, and finally late gene expression (.). Although such a temporal cascade of viral gene expression involves the action of virally encoded regulatory proteins, such factors act, at least in part, by interacting with cellular transcription factors that作者: 仇恨 時(shí)間: 2025-3-28 07:08 作者: 有害處 時(shí)間: 2025-3-28 10:59 作者: ANA 時(shí)間: 2025-3-28 16:04
Herpes Simplex Virus Protocols978-1-59259-594-5Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: immunity 時(shí)間: 2025-3-28 20:08 作者: URN 時(shí)間: 2025-3-28 23:36 作者: 冷峻 時(shí)間: 2025-3-29 06:34 作者: 熱情贊揚(yáng) 時(shí)間: 2025-3-29 09:32 作者: SLAG 時(shí)間: 2025-3-29 12:55
arkedly higher firearm homicide rate in the U.S. compared to other industrialized countries. In the U.S., firearm homicide rates are 12 to 16 times higher than for the average of 25 other industrialized countries, a statistic that has been related to U.S. laws pertaining to gun ownership (Dodge et al., 2006).作者: CAND 時(shí)間: 2025-3-29 17:34
Christine A. MacLeanerationally defined and distinguished by some sort of experiment, however far-fetched. In view of the title given to our group, we took it as our brief to look at the real tasks which animals perform in nature to see if they tell us anything about the rules or algorithms which animals actually use.作者: 彎曲道理 時(shí)間: 2025-3-29 23:29
Alasdair R. MacLeant’s account is not based on empirical research, unlike modern theories of animal psychology, his commentary shows interesting parallels to contemporary views and it is undoubtedly a substantial and important contribution to medieval zoology.作者: LATE 時(shí)間: 2025-3-30 01:48
Robert S. Coffinerceptual contents? Differently speaking, does the sheep see wolves rather than grey furry things? If it does, can it also combine various concepts and form propositions which are true or false? Questions as these puzzled many thirteenth-century thinkers, and John Blund was one of the first to provide an answer to them.作者: insomnia 時(shí)間: 2025-3-30 04:09
In Vitro Systems to Analyze HSV Transcript Processing,tional (IE63) levels to regulate early and late gene expression. Throughout infection, mRNA is synthesized using cellular RNA poly-merase II, which is modified by the action of an immediate early protein (.).作者: Glucocorticoids 時(shí)間: 2025-3-30 11:07 作者: FOIL 時(shí)間: 2025-3-30 16:02 作者: 溫和女人 時(shí)間: 2025-3-30 20:21
Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions,n vitro and infected with HSV-1 or to isolated nuclei of infected cells, to nuclear or cytoplasmic fractions, and, in some instances, to purified extracts of infected eukaryotic cells or of prokaryotic cells expressing a viral gene.作者: 一窩小鳥 時(shí)間: 2025-3-30 21:14 作者: larder 時(shí)間: 2025-3-31 03:57
Book 1998pefully, it will also provide information for those with experience in the field, as well as those embarking on techniques that are new to them. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely us作者: 洞察力 時(shí)間: 2025-3-31 07:31 作者: Hyperplasia 時(shí)間: 2025-3-31 09:11 作者: Apoptosis 時(shí)間: 2025-3-31 17:05
HSV Mutagenesis,ctious progeny virions are assembled and released. Exploring the function of the many herpes-encoded proteins and the mechanisms to control their expression during these processes thus requires a fine dissection of the herpes genome, so as to allow protein-coding regions to be linked with function and control DNA regions to be identified.作者: infantile 時(shí)間: 2025-3-31 21:12 作者: Minutes 時(shí)間: 2025-4-1 00:23 作者: 黑豹 時(shí)間: 2025-4-1 04:16 作者: 不足的東西 時(shí)間: 2025-4-1 09:54 作者: DEAF 時(shí)間: 2025-4-1 13:17
Lars Haarr,Nina Langeland in the successful .Neuromethods. series, the chapters provide authoritative reviews of the most commonly used approaches in the field...Vital and easy to use, .Animal Models for Retinal Diseases. serves to sup978-1-4939-5750-7978-1-60761-541-5Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: malapropism 時(shí)間: 2025-4-1 15:03
Ben Luisi,Marie Anderson,Graham Hope in the successful .Neuromethods. series, the chapters provide authoritative reviews of the most commonly used approaches in the field...Vital and easy to use, .Animal Models for Retinal Diseases. serves to sup978-1-4939-5750-7978-1-60761-541-5Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: Yourself 時(shí)間: 2025-4-1 18:54
Jordan G. Spivack in the successful .Neuromethods. series, the chapters provide authoritative reviews of the most commonly used approaches in the field...Vital and easy to use, .Animal Models for Retinal Diseases. serves to sup978-1-4939-5750-7978-1-60761-541-5Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: Uncultured 時(shí)間: 2025-4-2 00:31
John A. Blaho,Bernard Roizman in the successful .Neuromethods. series, the chapters provide authoritative reviews of the most commonly used approaches in the field...Vital and easy to use, .Animal Models for Retinal Diseases. serves to sup978-1-4939-5750-7978-1-60761-541-5Series ISSN 0893-2336 Series E-ISSN 1940-6045
