標題: Titlebook: Hepatocytes; Methods and Protocol Naoki Tanimizu Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri [打印本頁] 作者: 相持不下 時間: 2025-3-21 17:20
書目名稱Hepatocytes影響因子(影響力)
書目名稱Hepatocytes影響因子(影響力)學(xué)科排名
書目名稱Hepatocytes網(wǎng)絡(luò)公開度
書目名稱Hepatocytes網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Hepatocytes被引頻次
書目名稱Hepatocytes被引頻次學(xué)科排名
書目名稱Hepatocytes年度引用
書目名稱Hepatocytes年度引用學(xué)科排名
書目名稱Hepatocytes讀者反饋
書目名稱Hepatocytes讀者反饋學(xué)科排名
作者: cunning 時間: 2025-3-21 21:18
1064-3745 ation advice from the experts.This volume details experimental protocols to study structure and function of hepatocytes?.in vivo.?and?.in vitro., and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte s作者: Neuropeptides 時間: 2025-3-22 03:19
Misao Himeno,Shin-Wei Chen,Taketomo Kido on “ageing” by mapping the prevalent themes of research, research hotspots, and influential stakeholders. The findings have implications for researchers into ageing as well as policymakers across countries included in this volume and beyond.作者: BURSA 時間: 2025-3-22 05:56 作者: Boycott 時間: 2025-3-22 09:33 作者: 赤字 時間: 2025-3-22 13:49 作者: Surgeon 時間: 2025-3-22 17:20
Akinori Takemura,Kousei Itopment and the fight against poverty and social exclusion remains a priority. This chapter provides some insights into the new challenges for the nation by focusing on the financial security of the elderly as well as some elements of healthy ageing.作者: projectile 時間: 2025-3-22 22:29 作者: Headstrong 時間: 2025-3-23 01:43 作者: 繁殖 時間: 2025-3-23 07:28 作者: CHOIR 時間: 2025-3-23 11:55
Lavinija Matakovic,Arend W. Overeem,Karin Klappe,Sven C. D. van IJzendoornenerational inequality, and (3) gender inequality. Possible implications of the rapid population ageing on these societal inequities and potential ways forward in relation to two of the United Nations Sustainable Development Goals (SDGs) – SDG5 gender equality and SDG10 reduce inequality within countries, are also discussed.作者: CEDE 時間: 2025-3-23 15:28 作者: 不自然 時間: 2025-3-23 20:01
Katherine Shortt,Daniel P. Heruthering gap structures are selected as the research objects, including the insulator parallel gaps, transmission line-tower body or window gaps and complex gaps for helicopter live-line work. The proposed SVM model is applied to predict their discharge voltages.作者: Repetitions 時間: 2025-3-24 01:44 作者: Servile 時間: 2025-3-24 02:54 作者: addition 時間: 2025-3-24 10:09 作者: Anemia 時間: 2025-3-24 12:55
Evaluation of Urea Cycle Activity by Metabolic Flux Analysis Using Mass Spectrometry,s conducting specific, objective, and quantitative measurement of the activated state of the target metabolic pathway regardless of external disturbing factors. This section describes the technical background and methodology of performing metabolic flux analysis of the urea cycle by mass spectrometry.作者: exigent 時間: 2025-3-24 15:31 作者: fledged 時間: 2025-3-24 20:00 作者: BADGE 時間: 2025-3-25 00:10 作者: 蛙鳴聲 時間: 2025-3-25 04:58 作者: gratify 時間: 2025-3-25 08:05 作者: 阻擋 時間: 2025-3-25 13:31 作者: heterodox 時間: 2025-3-25 19:25
Candice Ashmore-Harris,Gilbert O. Fruhwirthr of the manga’s Germany-centric plots were included in the 39 stories selected for animation. The television episodes faithfully reproduced the original illustrations and dialogues created a decade earlier, and were aimed at the same mature audience for which the manga had been written.作者: 我悲傷 時間: 2025-3-25 23:09
Yinhua Jin,Teni Anbarchian,Roel Nusserate-based cultivation methods. In this book chapter, we discuss the formulation and commercialization of AMF biofertilizers, including the attributes that endanger them. One critical challenge in the commercialization of AMF formulations is quality assurance during every step of the process.作者: Arctic 時間: 2025-3-26 03:07
Norihisa Ichinohe,Naoki Tanimizu,Toshihiro Mitakansumption. A small effort has been made here to attract entrepreneurs to enter agribusiness and contribute to maintaining sustainability along with money making. This chapter deals with the technological details of mass production and the cost-benefit ratio to establish a small-scale Bt production unit.作者: blithe 時間: 2025-3-26 04:56 作者: 狼群 時間: 2025-3-26 10:21
Assessment of Hepatocyte Ploidy by Flow Cytometry,se liver through a two-step perfusion system for analysis of cellular ploidy. In an alternative approach, we employ a nuclei isolation protocol to assess nuclear ploidy. Finally, we describe how the use of fluorescent cell markers is compatible with these methods and helps retain information on cellular position within the tissue.作者: nuclear-tests 時間: 2025-3-26 16:33
Quantification of Clonal Expansion of Hepatocytes in Normal and Injured Liver,n be used to answer questions such as which hepatocytes contribute to hepatocyte renewal during homeostasis and during regeneration after injury, as well as how much individual hepatocytes in the different zones of the liver divide.作者: Isometric 時間: 2025-3-26 19:11 作者: 浮雕寶石 時間: 2025-3-27 00:02 作者: 季雨 時間: 2025-3-27 01:23
Hepatocytes978-1-0716-2557-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Robust 時間: 2025-3-27 08:37
Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations,Collagen sandwich culture of hepatocytes retains many in vivo-like properties and is used for many investigations in liver cell biology, and hepatic pharmacology and toxicology. This chapter describes the method of establishing collagen sandwich culture of hepatocytes in a glass bottom dish for image-based studies.作者: enmesh 時間: 2025-3-27 11:58
Feng Chene implied assumption that the parties have not by agreement extended the scope of the Convention. But, as has already been pointed out,. it is not uncommon that the owner and the charterer agree that the Warsaw Rules shall apply to a contract which would not automatically have been governed by them.作者: BUDGE 時間: 2025-3-27 15:54
Yuji Suzuki,Tokio Sasaki,Keisuke Kakisaka,Hiroaki Abe,Yasuhiro Takikawa done by discussing some practical situations in order to find out where the Warsaw Convention, according to our interpretation, applies. The intention is by no means to cover all the possible combinations of circumstances and arrange the material systematically, but only to pick out some situations that seem practical or otherwise interesting.作者: byline 時間: 2025-3-27 21:44
Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells, This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.作者: 煩人 時間: 2025-3-27 23:02
Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver Progenitor Cells and Livearious liver cells. We have already developed culture systems to generate liver progenitor cells (LPCs), hepatocytes, and liver sinusoidal endothelial cells (LSECs) from human induced pluripotent cells (iPSCs). Using those cells, we established a co-culture system to study HBV infection and replication in vitro.作者: 兇兆 時間: 2025-3-28 03:06 作者: infelicitous 時間: 2025-3-28 10:17 作者: subacute 時間: 2025-3-28 14:05 作者: GENRE 時間: 2025-3-28 17:32 作者: 亞當心理陰影 時間: 2025-3-28 22:16
Giuseppe Pettinato in the choice to focus on the Wall as an object since the resistant material quality of a reinforced concrete barrier influences the film’s rhythm more than the galloping pace of the events that overtook east European socialism? Nowadays, it is taken for granted that events must come fast, furious,作者: Expertise 時間: 2025-3-29 02:07
Laura Morán,Marius Maximilian Woitok,Matthias Bartneck,Francisco Javier Cuberore likely to achieve the UN Sustainable Development Goals for older Singaporeans. A central argument developed in this chapter is that these investments in healthcare may address the social protection needs of current retirees, but are unlikely to do so for future cohorts. The chapter concludes by o作者: septicemia 時間: 2025-3-29 03:10 作者: Critical 時間: 2025-3-29 09:21
Dong Fuombined knowledge from the fields of molecular biology and microbiology has opened new avenues in pharmacology and therapeutics and has given rise to new approaches for synthesizing drugs, namely the large-scale synthesis of proteins, peptides, factors, that have been found to have therapeutic poten作者: 嚴厲批評 時間: 2025-3-29 14:13 作者: 偽善 時間: 2025-3-29 17:33 作者: 暫時過來 時間: 2025-3-29 20:00
Generation of In Vivo Traceable Hepatocyte-Like Cells from Human iPSCs,e compatible with further maturation following transgene expression either in vitro or in vivo, with expression of the transgene retained. We detail how transgenic cells can be imaged in vivo. While we provide a protocol for the NIS reporter gene, the cell engineering aspects of this protocol are tr作者: subordinate 時間: 2025-3-30 02:01
,Long-Term Expansion of Murine?Primary Hepatocyte Organoids,cological studies, as well as for applications in regenerative medicine such as cell transplantation for the treatment of metabolic liver diseases. Here, we describe a protocol for the isolation and in vitro culture of primary hepatocytes in a three-dimensional extracellular matrix gel. The inflamma作者: inconceivable 時間: 2025-3-30 05:48
Generation of In Vivo Traceable Hepatocyte-Like Cells from Human iPSCs,r transduction with a lentivirus for gene transfer. Here, we engineer them to express the human sodium iodide symporter, which can be exploited as a radionuclide reporter gene, thereby enabling these cells to be tracked in vivo by single-photon emission computed tomography (SPECT) or positron emissi作者: 鳥籠 時間: 2025-3-30 11:15
Generation of Hepatocyte Organoids from Human iPS Cells, for a number of degenerative diseases. In this chapter, I describe a strategy to mass-produce fully functional hepatocyte organoids using hiPSCs interlaced with human adipose microvascular endothelial cells (HAMEC). Our unique technology employs a two-step strategy, consisting of the scalable gener作者: 值得尊敬 時間: 2025-3-30 14:12 作者: AWL 時間: 2025-3-30 19:32 作者: 預(yù)定 時間: 2025-3-31 00:16 作者: oxidant 時間: 2025-3-31 03:05
Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver Progenitor Cells and Livearious liver cells. We have already developed culture systems to generate liver progenitor cells (LPCs), hepatocytes, and liver sinusoidal endothelial cells (LSECs) from human induced pluripotent cells (iPSCs). Using those cells, we established a co-culture system to study HBV infection and replicat作者: 泄露 時間: 2025-3-31 07:42
In Vitro Assay System to Detect Drug-Induced Bile Acid-Dependent Cytotoxicity Using Hepatocytes,n vitro assay system to detect bile acid-dependent cytotoxicity in hepatocytes. This cell-based system can assess the toxicity of the parent compound, as well as the contribution of metabolite(s). In addition, this system can utilize several types of hepatocytes (primary hepatocytes, hepatoma cell l作者: hemophilia 時間: 2025-3-31 13:03 作者: RADE 時間: 2025-3-31 14:03
Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes,on recombinant adenovirus-mediated infection combined with a pulse-chase regimen and live-cell imaging analysis of fluorescent protein-tagged dipeptidyl peptidase IV (DPPIV) and vesicular stomatitis virus G (VSVG) cargo proteins, coexpressed and accumulated in the endoplasmic reticulum via DPPIV agg作者: Obituary 時間: 2025-3-31 19:35
Assessment of Hepatocyte Ploidy by Flow Cytometry,in the distribution of diploid and polyploid cells in the liver can be indicative of tissue stress or a pathologic state. Here, we describe a method for flow cytometric quantification of ploidy distribution by staining with propidium iodide. We first outline a hepatocyte isolation procedure from mou作者: 攝取 時間: 2025-4-1 00:30
Isolation of Small Hepatocyte-Like Progenitor Cells from Retrorsine/Partial Hepatectomy Rat Livers ers treated with retrorsine (Ret) and a 70% partial hepatectomy (PH). The Ret/PH model has been used widely to analyze the effectiveness of cell transplantation and the mechanisms of LSPC proliferation. Laser microdissection (LMD) is a powerful tool that can excise and collect specific areas of cell作者: innate 時間: 2025-4-1 04:15
Application of EdU-Based DNA Synthesis Assay to Measure Hepatocyte Proliferation In Situ During Livules and pathways as well as the regulatory mechanisms involved in liver regeneration. Periodic measurement of the liver/body mass ratio estimates the rate of liver regeneration, which is often supplemented by evaluating the proportion of proliferating hepatocytes using a synthetic nucleoside analog作者: Jubilation 時間: 2025-4-1 09:18 作者: 津貼 時間: 2025-4-1 14:15 作者: 路標 時間: 2025-4-1 15:45
Identification of Genes Regulating Hepatocyte Injury by a Genome-Wide CRISPR-Cas9 Screen,ene editing can be employed to study directly the functional impact of stable gene knockout, activation, and knockdown. Here, we describe the end-to-end methodology by which we employ genome-wide CRISPR-Cas9 knockout to study drug toxicity using acetaminophen (APAP) in a hepatocellular carcinoma liv
