標(biāo)題: Titlebook: Hepatitis B Virus; Methods and Protocol Haitao Guo,Andrea Cuconati Book 2017 Springer Science+Business Media LLC 2017 DNA and RNA viruses.c [打印本頁] 作者: 聯(lián)系 時(shí)間: 2025-3-21 19:59
書目名稱Hepatitis B Virus影響因子(影響力)
書目名稱Hepatitis B Virus影響因子(影響力)學(xué)科排名
書目名稱Hepatitis B Virus網(wǎng)絡(luò)公開度
書目名稱Hepatitis B Virus網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Hepatitis B Virus被引頻次
書目名稱Hepatitis B Virus被引頻次學(xué)科排名
書目名稱Hepatitis B Virus年度引用
書目名稱Hepatitis B Virus年度引用學(xué)科排名
書目名稱Hepatitis B Virus讀者反饋
書目名稱Hepatitis B Virus讀者反饋學(xué)科排名
作者: 拋物線 時(shí)間: 2025-3-21 21:16 作者: SENT 時(shí)間: 2025-3-22 00:33 作者: 小畫像 時(shí)間: 2025-3-22 06:52 作者: mutineer 時(shí)間: 2025-3-22 12:01
Yinyan Sun,Yonghe Qi,Bo Peng,Wenhui Liexamination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice.Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.作者: Cpap155 時(shí)間: 2025-3-22 14:59
Yi Ni,Stephan Urbanexamination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice.Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.作者: LEVY 時(shí)間: 2025-3-22 20:29 作者: agglomerate 時(shí)間: 2025-3-22 22:51 作者: 蝕刻 時(shí)間: 2025-3-23 02:22
Ching-Chun Yang,Hung-Cheng Li,Chiaho Shihexamination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice.Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.作者: 輕浮思想 時(shí)間: 2025-3-23 09:17 作者: 維持 時(shí)間: 2025-3-23 16:50
Thomas Tu,Allison R. Jilbertexamination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice.Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.作者: 使服水土 時(shí)間: 2025-3-23 19:32 作者: phase-2-enzyme 時(shí)間: 2025-3-24 00:44
Lena Allweiss,Marc Lütgehetmann,Maura Dandriexamination of disease etiology, pathogenesis, and pathophysiology and their correlation to clinical practice.Forefront knowledge of the mediators and mechanisms of anaphylaxis is covered with an emphasis on how new discoveries shape our current and emerging therapies.作者: 合乎習(xí)俗 時(shí)間: 2025-3-24 05:51 作者: stress-test 時(shí)間: 2025-3-24 06:48
Intracytoplasmic Transport of Hepatitis B Virus Capsids,..Here, we describe protocols that can be applied to investigations of the HBV capsid with the cytoplasmic transport systems. We have chosen to present two independent experimental approaches, which allow avoiding artifacts. Aside of the specific capsid detection system, the protocols can be applied to any other viral structure.作者: 使激動(dòng) 時(shí)間: 2025-3-24 11:33 作者: 無彈性 時(shí)間: 2025-3-24 16:38
1064-3745 ation advice from the experts.Includes supplementary materia.This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). .Hepatitis B Virus: Methods and Protocols. details protocols and techniques ranging from cell culture studies to in vivo and 作者: LEVER 時(shí)間: 2025-3-24 20:25
Ran Yan,Dawei Cai,Yuanjie Liu,Haitao Guo Ph.D.ositiv aufgreifen und um eine neue Wendung komplettieren: Mittels der n?heren Explikation des avantgardistischen bzw. genauer: des dadaistischen Anarchie-Begriffs lassen sich die entsprechenden, dazugeh?rigen ?sthetischen Werke (hier: die literarischen, sprach-künstlerischen Arbeiten) neu lesen und einer Lektüre unterziehen.作者: Interlocking 時(shí)間: 2025-3-25 02:24
Quan-Xin Long,Jie-Li Hu,Ai-Long Huangsowie die vereinfachende, skizzenhafte Darstellungsweise dieser Gem?lde, die die Ausstellungsbesucher überraschten. Dieser Bruch mit der akademischen Malerei veranlasste den Kritiker Louis Vauxcelles (1870–1943) dazu, die Künstler als ?Fauves? zu bezeichnen.作者: molest 時(shí)間: 2025-3-25 05:49 作者: Spangle 時(shí)間: 2025-3-25 10:08 作者: 仔細(xì)檢查 時(shí)間: 2025-3-25 12:13 作者: 脖子 時(shí)間: 2025-3-25 17:02
Hydrodynamic HBV Transfection Mouse Model,n to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.作者: TRAWL 時(shí)間: 2025-3-25 20:38
Cally D. Goddard,Lale Bildrici-Ertekin,Xiaohe Wang,Andrea Cuconati Ph.D.it befreit sich von den Zw?ngen der Repr?sentation und des ?guten Geschmacks?, um das Absto?ende, aber zugleich Begehrte zu ?sthetisieren. Diese Formen ahmen keine Vorbilder nach, sie repr?sentieren keine ihnen ?u?ere Realit?t, sondern etablieren sich als Trugbilder, die Verwirrung im Geiste und im Leiblichen hervorrufen.作者: 廚房里面 時(shí)間: 2025-3-26 00:18
Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay,immunoassay (EIA) and hybridization, respectively. Such convenient experimental procedure is called HBV particle assay and described in detail in this chapter. The particle gel assay can be used to study viral and host regulations of HBV virus morphogenesis and egress, and for antiviral assessment of HBV inhibitors as well.作者: 可卡 時(shí)間: 2025-3-26 04:58
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Char Two alternative approaches are used to render the cloned HBV genome replication competent: release and circularization of the 3.2-kb HBV genome prior to each transfection experiment or conversion of the monomeric clone into a tandem dimer version.作者: 艦旗 時(shí)間: 2025-3-26 09:39
Book 2017tocols. details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular traffickin作者: Flatus 時(shí)間: 2025-3-26 16:40 作者: Cholecystokinin 時(shí)間: 2025-3-26 16:50
Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/425698.jpg作者: 松緊帶 時(shí)間: 2025-3-27 00:34
Jessica C. Casciano,Michael J. BouchardBevor auf die theoretischen Grundlagen für die Analyse des h?ndlertypenbezogenen Marketing eingegangen wird, soll der Zusammenhang des nachfolgenden Theorieteils als Grundlage für die Erarbeitung der h?ndlerspezifischen Marketingstrategien kurz dargestellt werden.作者: 直覺好 時(shí)間: 2025-3-27 05:09 作者: Arresting 時(shí)間: 2025-3-27 09:02 作者: Geyser 時(shí)間: 2025-3-27 12:10 作者: 母豬 時(shí)間: 2025-3-27 14:31
978-1-4939-8277-6Springer Science+Business Media LLC 2017作者: Trypsin 時(shí)間: 2025-3-27 21:03
Hepatitis B Virus978-1-4939-6700-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: DUST 時(shí)間: 2025-3-28 00:34
Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications,ve protocols for 96-well format sandwich ELISA assays for this purpose, in which there are many options for customization of antibodies used and other parameters. These protocols can be adapted to use for animal serum samples, compound library screening, and other purposes.作者: 數(shù)量 時(shí)間: 2025-3-28 04:14
Book 2017 introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Hepatitis B Virus: Methods and Protocols. is a valuable tool fo作者: 失敗主義者 時(shí)間: 2025-3-28 06:15
NTCP-Reconstituted In Vitro HBV Infection System,eneration and selection of single cell clones of HepG2-NTCP; production of infectious HBV virion stock through DNA transfection of recombinant plasmid that enables studying primary clinical HBV isolates; and assessing the infection with immunostaining of HBV antigens and Southern blot analysis of HB作者: 領(lǐng)先 時(shí)間: 2025-3-28 13:45 作者: Insulin 時(shí)間: 2025-3-28 16:35 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-28 19:02
A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA,nuclease, T7EI. Assays employing this enzyme entail initial amplification of DNA encompassing the targeted region. Thereafter the amplicons are denatured and reannealed to allow hybridization between indel-containing and wild-type sequences. Heteroduplexes that contain mismatched regions are suscept作者: AORTA 時(shí)間: 2025-3-28 23:21
Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA Using Inverse Nested PCRBV-associated liver disease, suggesting that hepatocytes with a survival or growth advantage undergo high levels of clonal expansion even in the absence of oncogenic transformation. Here we describe inverse nested PCR (invPCR), a highly sensitive method that allows detection, sequencing, and enumera作者: breadth 時(shí)間: 2025-3-29 06:42 作者: 宴會(huì) 時(shí)間: 2025-3-29 11:07
Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytsis and the role of HBx in HBV replication. Here, we describe a single-cell Ca.-imaging protocol that we use to investigate HBV and HBx effects on the level of cytosolic Ca.. We specifically outline two methods that we use to evaluate HBV and HBx regulation of cytosolic Ca. levels in cultured primar作者: FOLD 時(shí)間: 2025-3-29 11:29 作者: OMIT 時(shí)間: 2025-3-29 17:34 作者: LURE 時(shí)間: 2025-3-29 21:15
Yanli Qin,Yong-Xiang Wang,Jiming Zhang,Jisu Li,Shuping Tongerdings nicht trennscharf differenzieren, weshalb sie in der Forschungsliteratur und auch im vorliegenden Beitrag synonym verwendet werden: ?Anarchie? und ?Anarchismus? und ihre jeweiligen Adjektive sind eng miteinander verschr?nkte Beschreibungskategorien sowohl innerhalb eines (gesellschafts) poli作者: Disk199 時(shí)間: 2025-3-30 03:23
NTCP-Reconstituted In Vitro HBV Infection System,in human hepatoma HepG2 cells (HepG2-NTCP) renders these cells susceptible for HBV infection. The HepG2-NTCP stably transfected cell line provides a much-needed and easily accessible platform for studying the virus. HepG2-NTCP cells could also be used to identify chemicals targeting key steps of the作者: neurologist 時(shí)間: 2025-3-30 07:23 作者: CLAM 時(shí)間: 2025-3-30 11:30 作者: 思想 時(shí)間: 2025-3-30 16:06
Intracytoplasmic Transport of Hepatitis B Virus Capsids,o be transported through the cytoplasm to the nuclear periphery, followed by nuclear entry. These steps have to take place in a coordinated manner to allow delivery of the genome into the nucleus. Due to the viscosity of the cytoplasm, the intracytoplasmic translocation has to be active and directed作者: Outwit 時(shí)間: 2025-3-30 19:35 作者: 前兆 時(shí)間: 2025-3-31 00:07 作者: 最初 時(shí)間: 2025-3-31 04:14
A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA,iral infections such as persistant hepatitis B virus (HBV). The replication intermediate of HBV comprising covalently closed circular DNA (cccDNA) is stable and resistant to available licensed antiviral agents. Advancing gene editing as a means of introducing targeted mutations into cccDNA thus pote作者: TOXIN 時(shí)間: 2025-3-31 08:08
Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA Using Inverse Nested PCRworldwide. Many of the steps that occur during progression from the normal liver to cirrhosis and/or HCC are unknown. Integration of HBV DNA into random sites in the host cell genome occurs as a by-product of the HBV replication cycle and forms a unique junction between virus and cellular DNA. Analy作者: NEG 時(shí)間: 2025-3-31 11:52 作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-31 16:18 作者: 一夫一妻制 時(shí)間: 2025-3-31 17:43 作者: 辭職 時(shí)間: 2025-3-31 22:55 作者: Filibuster 時(shí)間: 2025-4-1 03:57
In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors,e interferon α and nucleos(t)ide analogs have insufficient efficiency, are of long duration and can be accompanied by systemic side effects. Though HBV RNaseH is essential for viral replication, it is unexploited as a drug target against HBV. RNaseH inhibitors that actively block viral replication w作者: 思想 時(shí)間: 2025-4-1 08:13
Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay, infectious virions, genome-free virion, envelope-only subviral particles, and nonenveloped naked capsids. Based on their different physical and chemical properties, the enveloped and nonenveloped particles can be separated by the native agarose gel electrophoresis and transferred onto a positively 作者: Maximize 時(shí)間: 2025-4-1 13:21
Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications,ve protocols for 96-well format sandwich ELISA assays for this purpose, in which there are many options for customization of antibodies used and other parameters. These protocols can be adapted to use for animal serum samples, compound library screening, and other purposes.作者: 不開心 時(shí)間: 2025-4-1 18:11
Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of encing was previously the classic approach for quasispecies analysis, but this method was also time-consuming and laborious. Ultra-deep sequencing has been widely used in viral quasispecies research, especially for low-frequency mutation detection. Here we present a multiple samples deep sequencing 作者: Aerophagia 時(shí)間: 2025-4-1 22:06
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Char hepatoma cell lines can establish their biological properties to shed light on their different pathogenic potentials, yet very few clinical HBV isolates have been functionally characterized so far. The technical challenges include faithful amplification of the full-length HBV genome from clinical s作者: Munificent 時(shí)間: 2025-4-2 00:12
Hydrodynamic HBV Transfection Mouse Model,re delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles 作者: cunning 時(shí)間: 2025-4-2 06:43
is book fills the gaps in our understanding of the identification of triggers, recognition of clinical presentations, understanding of the natural history of these reactions, and selection of treatment strategies including those focused on cellular and molecular targets.The book provides a detailed
