標(biāo)題: Titlebook: Hemostasis and Thrombosis Protocols; David J. Perry,K. J. Pasi Book 1999 Springer Science+Business Media New York 1999 [打印本頁(yè)] 作者: CRUST 時(shí)間: 2025-3-21 19:52
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作者: HALL 時(shí)間: 2025-3-21 20:41
Amplification of DNA and RNA by PCRof the chapters employ the technique at some point to amplify specific DNA or RNA sequences. This chapter, therefore, provides a brief outline of the techniques and methods for the amplification of both DNA and RNA. It should be remembered that no single protocol will be suitable for all PCR reactio作者: NIB 時(shí)間: 2025-3-22 01:19
Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beadsf the amplification primers and as a result becomes incorporated into PCR product during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobi作者: Maximizer 時(shí)間: 2025-3-22 07:05 作者: Vulnerary 時(shí)間: 2025-3-22 09:48
Detection of DNA by Silver Stainingtages over conventional staining methods, including the ability to detect very small amounts of material, while avoiding the hazards of other detection systems, such as ethidium bromide or radio-isotopes.作者: Gustatory 時(shí)間: 2025-3-22 15:09
Promoter Studies in Hemostasised the production of cloned genomic material encompassing the putative promoter regions of genes involved in the hemostatic process. These recombinant vectors provide the raw material for reporter gene studies and DNA footprinting analysis; two of the three most frequently used in vitro procedures t作者: Brain-Waves 時(shí)間: 2025-3-22 18:29
Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoretions and polymorphisms. The most frequently used screening methods for mutation hunting in bleeding disorders are denaturing gradient gel electrophoresis (DGGE) (.), single-strand conformation polymorphism (SSCP) (.), and chemical mismatch cleavage (.). This chapter gives an introduction to the the作者: Offset 時(shí)間: 2025-3-22 23:06 作者: 致命 時(shí)間: 2025-3-23 03:32 作者: Endearing 時(shí)間: 2025-3-23 05:51 作者: 預(yù)兆好 時(shí)間: 2025-3-23 10:19
Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000? and MDE? Gel Electrophorver, direct sequencing of this amplified material is tedious, time-consuming, and frequently generates large amounts of normal sequence data. Based on the PCR technique, a number of screening methods for detecting DNA mutations have been developed and a number of these are described in this volume. 作者: 叢林 時(shí)間: 2025-3-23 17:19
tion and derivation of the most popular models of the firm. Intended for third-year undergraduate students of business finance, quantitative finance, and financial mathematics, as well as first-year postgraduate students, it is based on the twin pillars of theory and analytics, which merge in a way 作者: nonchalance 時(shí)間: 2025-3-23 20:36 作者: 談判 時(shí)間: 2025-3-23 22:21
there is a trade-off between these two aims. The theory of the choices under uncertainty leads the decision-making process in capital markets. The aim is to analyse the behaviour of the rational investor under uncertainty. Specifically, the aim of the theory is not to define a set of criteria for t作者: attenuate 時(shí)間: 2025-3-24 03:54
David J. Perryloan. There are several types of bonds. However, there are some elements common to all of them. In reality, any bond can be defined on the basis of five main elements: face value, price, coupon, maturity date, issuer. There is a strict relationship between price and risk of the bond. Specifically, t作者: 警告 時(shí)間: 2025-3-24 09:40 作者: 不足的東西 時(shí)間: 2025-3-24 10:58 作者: 慢慢沖刷 時(shí)間: 2025-3-24 16:02 作者: TRAWL 時(shí)間: 2025-3-24 22:14
Helen L. Devereuxraphic, economic, social and technological factors that have influenced and are influencing such changes (mobility drivers). Recent trends of the identified drivers have been analysed to draw possible development scenarios of passenger demand, forecast some likely effects on car mobility during the 作者: FRONT 時(shí)間: 2025-3-25 02:48
David J. Perry,Flora Peyvandirive for internationalisation has produced the immense traffic flows that feed it. The container is one of the many standardisation processes that make the industrialisation of maritime transport of general cargo possible. This form of transportation is used worldwide and therefore requires standard作者: 帽子 時(shí)間: 2025-3-25 05:11
Peter R. Winship,Jonathan R. K. Spraylso known as geo-visualisation, applied to support spatial decision making and planning. InViTo is based on the idea that interacting with data can improve the users knowledge process, while visualisation should contribute to increase intuitive perception. Therefore, through the interactive and visu作者: HUSH 時(shí)間: 2025-3-25 09:40
approach is particularly useful in situation characterized by an inter-connected range of environmental, social and economic issues. Moreover the presence of many actors with different backgrounds and knowledge constitutes a further level of complexity due to the difficulty in interpreting and read作者: Extemporize 時(shí)間: 2025-3-25 12:13
Rainer Schwaab,Winfried Schmidtms based on signature methods for root distribution determination. First, we present some basic results for the computation of stabilizing sets. Second, we provide justification and background for the computation of stabilizing sets. Then, we describe the procedure to compute the stabilizing set for作者: 跟隨 時(shí)間: 2025-3-25 18:56 作者: 宏偉 時(shí)間: 2025-3-25 22:32 作者: BOLT 時(shí)間: 2025-3-26 01:32
st: "No diagrams will be found in this work. The methods that I explain in it require neither constructions nor geometrical or mechanical arguments, but only the algebraic operations inherent to a regular and uniform process. Those who love Analysis will, with joy, see mechanics become a new branch 作者: 高爾夫 時(shí)間: 2025-3-26 07:02
Chike Ononye,P. Vincent Jenkinsl." Indeed, Lagrange made the following boast: "No diagrams will be found in this work. The methods that I explain in it require neither constructions nor geometrical or mechanical arguments, but only the algebraic operations inherent to a regular and uniform process. Those who love Analysis will, w作者: 終端 時(shí)間: 2025-3-26 08:47 作者: Recessive 時(shí)間: 2025-3-26 13:22 作者: 易于 時(shí)間: 2025-3-26 20:39
Chike Ononye,P. Vincent JenkinsIn this chapter, we consider the . optimal synthesis of digital PI and PID controllers. We present the computation of ., the set of stabilizing digital PI or PID controllers for a given plant, satisfying an . norm bound of . on the error transfer function.作者: 繞著哥哥問(wèn) 時(shí)間: 2025-3-26 23:09 作者: 舊病復(fù)發(fā) 時(shí)間: 2025-3-27 03:49 作者: 樹(shù)木中 時(shí)間: 2025-3-27 05:32
Direct Sequencing of PCR ProductsSequencing is an increasingly important part of the analysis of a polymerase chain reaction (PCR) product and is used in many areas of hemostasis. The protocols described in this chapter have been used to sequence many different PCR templates and provide high-quality, reproducible sequence data.作者: 多山 時(shí)間: 2025-3-27 11:49
Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern BlottingIntron 22 of the factor VIII gene contains a 9.5kb region of DNA that is repeated on at least two other locations telomeric to, and at least 500 kb from, the gene. These regions are termed intron 22 homologous regions (int22h) (.) and contain the Factor VIII associated gene (F8A).作者: 鈍劍 時(shí)間: 2025-3-27 14:10
Methods in Molecular Medicinehttp://image.papertrans.cn/h/image/425624.jpg作者: 嬰兒 時(shí)間: 2025-3-27 21:10
Isolation of DNA and RNAmples for DNA isolation can be stored at ?50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats—the interface between the red cells and the plasma that is seen following centrifugation of whole blood. This latter method also allows isolation of the plasma fraction.作者: 松果 時(shí)間: 2025-3-27 23:21 作者: defile 時(shí)間: 2025-3-28 02:54 作者: 鄙視 時(shí)間: 2025-3-28 09:09 作者: 鍵琴 時(shí)間: 2025-3-28 13:03
Hemostasis and Thrombosis Protocols978-1-59259-248-7Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 江湖郎中 時(shí)間: 2025-3-28 16:50
David J. Perryl is still under intensive discussion. In this context, starting with the Modigliani and Miller theories, whose studies are considered the starting point of the modern theory of capital structure, the cost of equity, debt and company capital are estimated.作者: PLUMP 時(shí)間: 2025-3-28 22:22
Rainer Schwaab,Winfried Schmidt LTI systems with P, PI, and PID controllers, and first-order controllers without delay. Finally, we present the computation of the PID stabilizing set which assigns closed-loop poles with real parts less than ., for prescribed ..作者: 斜谷 時(shí)間: 2025-3-29 01:59
David J. Perry LTI systems with P, PI, and PID controllers, and first-order controllers without delay. Finally, we present the computation of the PID stabilizing set which assigns closed-loop poles with real parts less than ., for prescribed ..作者: Emg827 時(shí)間: 2025-3-29 05:21
Amplification of DNA and RNA by PCRns and that optimization of the reaction is necessary whenever a new PCR reaction is undertaken. Further information on PCR can be found in one of the many textbooks on the subject that are available.作者: Musket 時(shí)間: 2025-3-29 09:53
Promoter Studies in Hemostasiso study promoter function. The third of these methods to be described in this chapter, band shift or electrophoretic mobility shift assay (EMSA), normally uses synthetically produced double-stranded oligonucleotide sequences corresponding to specific areas of interest within the promoter region.作者: construct 時(shí)間: 2025-3-29 12:04
Book 1999ffect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assessed by meth作者: tendinitis 時(shí)間: 2025-3-29 17:35 作者: 恩惠 時(shí)間: 2025-3-29 20:33 作者: 禁止 時(shí)間: 2025-3-30 01:23 作者: choroid 時(shí)間: 2025-3-30 04:18
Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis ofigration during denaturing gel electrophoresis (.). Third, chemicals can be employed that bind to the mismatch site, allowing immunochemical labeling of the products for identification (.). All these techniques have their limitations and no one method will detect all mutations (.–.).作者: 津貼 時(shí)間: 2025-3-30 10:59
Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000? and MDE? Gel Electrophor in which one strand is derived from the normal allele and the other from the mutant or altered allele. The heteroduplexes can exhibit a variety of changes in their physical properties and can be readily detected by a variety of techniques. In the many cases, such heteroduplexes can be detected without the use of radiolabeling.作者: encomiast 時(shí)間: 2025-3-30 14:51
1543-1894 ers that affect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assess作者: 食料 時(shí)間: 2025-3-30 16:48 作者: FAR 時(shí)間: 2025-3-30 20:56
