標(biāo)題: Titlebook: Hematopoietic Stem Cells; Methods and Protocol Louis M. Pelus,Jonathan Hoggatt Book 2023 Springer Science+Business Media, LLC, part of Spri [打印本頁(yè)] 作者: 復(fù)雜 時(shí)間: 2025-3-21 16:24
書(shū)目名稱(chēng)Hematopoietic Stem Cells影響因子(影響力)
作者: disrupt 時(shí)間: 2025-3-21 23:36 作者: Esalate 時(shí)間: 2025-3-22 01:15
Detection of DNA Damage in Hematopoietic Stem Cellsall, the Comet and Halo assays are powerful means of directly measuring DNA damage. We recommend the below methods for detecting damage in hematopoietic stem cells, but the methods can easily be adjusted for measuring damage in any type of single cells in suspension.作者: degradation 時(shí)間: 2025-3-22 07:08
Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immuarly under stress, offers the potential for therapeutic intervention. We have utilized Chromatin immunoprecipitation (ChIP) in HSPCs to understand epigenetic regulation in response to ionizing radiation. This technique can be applied reliably to rare hematopoietic cells and offers a powerful tool to explore epigenetic regulation in HSPCs.作者: Exclude 時(shí)間: 2025-3-22 10:18 作者: crucial 時(shí)間: 2025-3-22 15:43 作者: 無(wú)法治愈 時(shí)間: 2025-3-22 19:22
Intravital Microscopy for Hematopoietic Studieshether specific niches exist for distinct developing hematopoietic lineages. Here, we describe our protocol and techniques used to visualize transplanted HSCs in the mouse calvarium, using combined confocal and two-photon IVM.作者: 重疊 時(shí)間: 2025-3-22 22:07 作者: 闡釋 時(shí)間: 2025-3-23 05:09 作者: CRUMB 時(shí)間: 2025-3-23 09:03 作者: 改正 時(shí)間: 2025-3-23 13:32
Saipriya Ayyar,Isabel Beermannt supporting our third methodological choice, the multiple correspondence analysis (MCA) technique (Section 3.3). Finally, Section 3.4 develops the MCA technique and illustrates it with a numerical case study on Vietnam.作者: Dictation 時(shí)間: 2025-3-23 17:02
MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Nicheir identification and characterization is described. In addition, a protocol for MSC and HSPC coculture assay is presented. This MSC-HSPC coculture assay can be used for ex vivo expansion of HSPC. Furthermore, this assay is also useful for qualitative analysis of MSCs capable of supporting hematopoiesis.作者: 慢慢流出 時(shí)間: 2025-3-23 20:04 作者: 記憶 時(shí)間: 2025-3-24 00:23
Reza Shahbazi,Patricia Lipson,Karthikeya S. V. Gottimukkala,Daniel D. Lane,Jennifer E. Adairns for the groups. It could serve as a baseline for evaluating the fit of more specific models. In principle, it allows for assessment of the dependence, but it may be inefficient compared to a fully parametric or semi-parametric estimate.作者: 向下五度才偏 時(shí)間: 2025-3-24 03:16 作者: 喚醒 時(shí)間: 2025-3-24 08:56
Book 2023ecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell func作者: Osteoarthritis 時(shí)間: 2025-3-24 11:23 作者: Myocarditis 時(shí)間: 2025-3-24 18:33
Detection of DNA Damage in Hematopoietic Stem Cells measurements with single-cell resolution. Though these techniques are prone to have variability, they can be robust tools for quantifying DNA damage when planned and executed carefully. Here, we present both assays and highlight each technique’s advantages and challenges in measuring DNA damage in 作者: 多節(jié) 時(shí)間: 2025-3-24 19:19 作者: 挫敗 時(shí)間: 2025-3-25 02:28 作者: 組裝 時(shí)間: 2025-3-25 07:18
Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cellsl animal model used to study hematopoietic stem and progenitor cell (HSPC) biology, with the capability of serving as a step for the translation of the basic research concepts from small animals to humans. Lentiviral vectors are currently the standard gene delivery vehicles for transduction of HSPCs作者: 愉快么 時(shí)間: 2025-3-25 11:14
Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cellsl models such as nonhuman primates (NHPs). Similarity in physiology, size, and life expectation as well as cross-reactivity of most reagents and medications allows for the development of treatment strategies with rapid translation to clinical applications. Especially after the adverse events of HSC 作者: 使痛苦 時(shí)間: 2025-3-25 11:45 作者: painkillers 時(shí)間: 2025-3-25 17:56
Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotypee array of different cell-cell interactions and cell signaling events. The overwhelming diversity of the components that can regulate hematopoiesis, especially when factoring in how the cell surface and intracellular protein expression profiles of hematopoietic stem/progenitor cells and niche compon作者: 遣返回國(guó) 時(shí)間: 2025-3-25 22:04
Hematopoietic Stem Cell Identification Postirradiationocesses, as well as the development of treatments to prevent hematopoietic damage or enhance recovery after radiation exposure, often require analysis of bone marrow cells early after irradiation. While flow cytometry methods are well characterized for identification and analysis of bone marrow popu作者: FLINT 時(shí)間: 2025-3-26 03:35
Intravital Microscopy for Hematopoietic Studiesas stromal cell components. Together they form the BM microenvironment or HSC niche. HSCs critically depend on signaling from these niches to function and survive in the long term. Significant advances in imaging technologies over the past decade have permitted the study of the BM microenvironment i作者: 可互換 時(shí)間: 2025-3-26 08:20
Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biologyoietic stem cells (HSCs) that give rise to all blood cells in the body. It is also the site of origin for malignant blood cells such as leukemia and multiple myeloma, as well as a frequent site of metastasis for many solid tumors including prostate and breast cancer. The following chapter describes 作者: 睨視 時(shí)間: 2025-3-26 11:04
MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Nichen ex vivo culture system that recapitulates the marrow microenvironment is important to understanding the niche’s regulatory role on HSPC function and improving ex vivo HSPC expansion for clinical transplantation. Herein, a procedure for ex vivo expansion of MSCs from human bone marrow cells and the作者: 慢跑 時(shí)間: 2025-3-26 15:07
Isolation of Thymus Stromal Cells from Human and Murine Tissueon. The thymic stroma secretes the factors that recruit and sustain T cell progenitors, and they also partake in the shaping of a functional and tolerant T cell receptor repertoire. The damage incurred to the thymic stromal compartment by bone marrow conditioning regimens as well as by the natural a作者: 公司 時(shí)間: 2025-3-26 17:26
Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantationm cells (HSC) from experimental mouse and human donor groups. These groups could include, but are not limited to, genetically altered populations (gene knockout/knockin models), ex vivo manipulated cell populations, or in vivo modulated cell populations. The basic fundamentals of this process involv作者: scotoma 時(shí)間: 2025-3-26 21:08
s include ever more sophisticated modeling options. The goal of this book is to familiarize readers with a wide range of commonly used models, and thereby to enable them to become critical consumers of current empirical research, and to conduct their own empirical analyses. The focus of the book is 作者: 蜈蚣 時(shí)間: 2025-3-27 03:00
ary text to an applied microeconomics field course (e. g. arThe availability of microdata has increased rapidly over the last decades, and standard statistical and econometric software packages for data analysis include ever more sophisticated modeling options. The goal of this book is to familiariz作者: 魯莽 時(shí)間: 2025-3-27 06:16
Jonathan Hoggatt,Louis M. Peluson of policies to combat it. The monetary approach is not sufficient to capture the multiple aspects of poverty: a multidimensional analysis is also needed. The main objective of this research is therefore to construct a composite indicator of poverty using a basic needs approach. The analysis shows作者: Autobiography 時(shí)間: 2025-3-27 09:38
ethodology aims to be practical with the use of computationaPoverty is a paradoxical state. Recognizable in the eld for any sensitive observer who travels in remote rural areas and urban slums and meets marginalized people in a given society, poverty still remains a challenge to conceptual formaliza作者: IRATE 時(shí)間: 2025-3-27 17:01
Saipriya Ayyar,Isabel Beermane and on a first methodological choice in Section 3.1, Section 3.2 presents a quick review of the main methodologies used to build a composite indicator of poverty (CIP). Our second methodological choice takes us to a short presentation of different variants of factorial approaches and to the argume作者: paleolithic 時(shí)間: 2025-3-27 21:34
Reza Shahbazi,Patricia Lipson,Karthikeya S. V. Gottimukkala,Daniel D. Lane,Jennifer E. Adairtional assumptions, but it does, of course, require some structural assumptions, namely, independence of groups and identical multivariate distributions for the groups. It could serve as a baseline for evaluating the fit of more specific models. In principle, it allows for assessment of the dependen作者: 勤勉 時(shí)間: 2025-3-28 00:47 作者: Reclaim 時(shí)間: 2025-3-28 03:07 作者: 反應(yīng) 時(shí)間: 2025-3-28 07:47 作者: Incumbent 時(shí)間: 2025-3-28 10:37
Myriam L. R. Haltalli,Cristina Lo Celsoution provides a sketch of the strategy we have adopted toward the brain-behavior relation, notes its main tenets and applies them to a new and very specific experiment that uses large scale SQuID arrays to determine how the human brain times individual actions to environmental events. A second pape作者: Between 時(shí)間: 2025-3-28 16:18 作者: Chivalrous 時(shí)間: 2025-3-28 20:38 作者: happiness 時(shí)間: 2025-3-29 01:40
Karin Gustafsson,David T. Scaddenoncerned with the analysis of EEG and MEG data. In these experiments, electric and/or magnetic fields are measured by means of arrays of sensors. The measurements consist in recording time-series of the corresponding fields. The spatial resolution is given by the separation of the sensors on the sca作者: 神刊 時(shí)間: 2025-3-29 03:49 作者: LAY 時(shí)間: 2025-3-29 11:10
Scott H. Cooper,Maegan L. Capitano,Hal E. Broxmeyery of plants undoubtedly played a major role in the long-standing popularity of caffeine-containing products, especially nonalcoholic beverages and foods (coffee, tea, soft drinks, cocoa and chocolate products, etc.). More than 60 plant species throughout the world have been identified as containing 作者: Osteoarthritis 時(shí)間: 2025-3-29 15:28
Book 2023tocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Hematopoietic Stem Cells: Methods and Protocols. serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it..作者: 鐵砧 時(shí)間: 2025-3-29 17:12
nditional probability models and marginal probability e?ects are introduced here as the unifying principle for modeling, estimating and interpreting microdata relationships. We consider the limitation to m- imum likelihood sensible, from a pedagogical point of view if the book is to be used in a sem作者: 惡臭 時(shí)間: 2025-3-29 23:40
Myriam L. R. Haltalli,Cristina Lo Celso of description for the paradigmatic case of bimanual coordination. Our collective goal in these three papers is to set the stage for a principled move from phenomenological laws at the behavioral level to the specific neural mechanisms that underlie them. With respect to the history of science our 作者: 解凍 時(shí)間: 2025-3-30 01:25 作者: 缺陷 時(shí)間: 2025-3-30 04:34 作者: SEVER 時(shí)間: 2025-3-30 09:04
ifying principle for modeling, estimating and interpreting microdata relationships. We consider the limitation to m- imum likelihood sensible, from a pedagogical point of view if the book is to be used in a sem978-3-540-29607-2作者: Scintigraphy 時(shí)間: 2025-3-30 14:28 作者: 過(guò)份艷麗 時(shí)間: 2025-3-30 17:36
the work of Cowan, Feldman, and Wilson (see sect. 2.2). The networks are described by systems of nonlinear integral equations. Therefore the paper can also be r978-3-540-09966-6978-3-642-45517-9Series ISSN 0341-633X Series E-ISSN 2196-9981
